• IMMUNE NETWORK
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• v.6 no.1
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• pp.6-12
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• 2006

Background: The Hypothalamo-Pituitary-Adrenal (HPA) axis is an important regulator for the body's stress response. As a primary stress responsive system, HPA-axis secretes various neurotransmitters, hormones, and cytokines, which regulates the immune system. Natural killer (NK) cell which is plays an important role in the innate immune response, is specially decreased their numbers and loose cytolytic activity in response to stress. However, the effect of HPA-axis secreted proteins on NK cell activity has not been defined. Herein, we studied the effect of adrenal secreted adiponectin on NK cell cytotoxicity. Adiponectin which is well-known metabolic control protein, plays important roles in various diseases, including hypertension, cardiovascular diseases, inflammatory disorders, and cancer. Methods: Signal sequence trap was used to find stress novel secretory protein from HP A-axis. Selected adiponectin was treated mouse mature primary NK cells and then examined the effect of adiponectin to NK cell cytotoxicity and cytokine expression level. Results: We found that adiponectin which is secreted from adrenal gland, suppress IL-2 induced NK cell cytotoxicity. And also investigated cytolytic cytokines are suppressed by adiponectin. Conclusion: These data suggest that adiponectin inhibites NK cell cytotoxicity via suppression of cytotoxicity related target gene.

• Journal of Veterinary Science
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• v.21 no.3
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• pp.42.1-42.22
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• 2020

Regenerative medicine using stem cells from various sources are emerging treatment modality in several refractory diseases in veterinary medicine. It is well-known that stem cells can differentiate into specific cell types, self-renew, and regenerate. In addition, the unique immunomodulatory effects of stem cells have made stem cell transplantation a promising option for treating a wide range of disease and injuries. Recently, the medical demands for companion animals have been rapidly increasing, and certain disease conditions require alternative treatment options. In this review, we focused on stem cell application research in companion animals including experimental models, case reports and clinical trials in dogs and cats. The clinical studies and therapeutic protocols were categorized, evaluated and summarized according to the organ systems involved. The results indicate that evidence for the effectiveness of cell-based treatment in specific diseases or organ systems is not yet conclusive. Nonetheless, stem cell therapy may be a realistic treatment option in the near future, therefore, considerable efforts are needed to find optimized cell sources, cell numbers and delivery methods in order to standardize treatment methods and evaluation processes.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.33 no.6
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• pp.435-442
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• 2009

Three-dimensional heat transfer characteristics for natural convection flows are numerically investigated in the doubly-inclined cubical-cavity according to the variation of a newly defined orientation angle �� of the hot wall surface from horizontal plane at moderate Rayleigh numbers. Numerical simulations of laminar flows are conducted in the range of Rayleigh numbers($10^4{\leq}Ra{\leq}10^5$) and $0^{\circ}{\leq}{\alpha}90^{circ}$ with a solution code(PowerCFD) employing unstructured cell-centered method. Comparisons of the average Nusselt number at the cold face are made with benchmark solutions and experimental results found in the literature. It is found that the average Nusselt number at the cold wall has a maximum value around the specified orientation ${\alpha}$ at each Rayleigh number. Special attention is also paid to three-dimensional thermal characteristics in natural convection according to new orientation angles at Ra��= $1{\times}10^5$, in order to investigate a new additional heat transfer characteristic found in the range of above Ra = $6{\times}10^4$.

• Food Science and Biotechnology
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• v.15 no.6
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• pp.866-870
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• 2006

The maintenance of physiological activity during dilution is very critical for the accurate enumeration of Vibrio spp. in marine samples. We investigated the effect of various diluents on the recovery of Vibrio parahaemolyticus using the direct plate counting and most probable number (MPN) methods. The effects of NaCl (0.85 and 3%) and pH (from 6.6 to 7.4) in diluents based on distilled water or phosphate buffered saline (PBS) were evaluated with three V. parahaemolyticus strains. PBS-3% NaCl (pH 6.6), as opposed to PBS, was the most effective diluent at maintaining viable cell numbers up to 2 log CFU/g during dilution for direct plate counting using on thiosulfate citrate bile salts sucrose (TCBS) selective agar, as well as minimizing the difference in cell numbers between TCBS and non-selective nutrient agar. It also increased counts of V parahaemolyticus inoculated into oysters relative to PBS (p<0.01), suggesting that PBS-3% NaCl (PH 6.6) can reduce the problem of underestimating V. parhaemolyticus counts using PBS alone.

• Journal of Korean Medical classics
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• v.18 no.2 s.29
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• pp.190-204
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• 2005

The purpose of this research is to discover changes of WBC and ANC numbers before and after applying Oriental medicine therapy to blood cancer patients. After that, appropriate music therapy method was well planned and carried for patients. Demonstration and music were conducted according to Ohaeng theory. The oriental music therapy was conducted three hours everyday by listening to music and we made patients participate in playing the instrument for one hour in two times a week. The result was verified in three ways by checking and comparing numbers of WBC, ratio of ANC and New Trophil before and after the experiment. In addition to that verification, we analyzed patients' survey and their response after treatment. The result was that WBC and ANC were efficient as p=0.0419, p=0.0262 each and the ratio of New trophil was not efficient in p=0.999, but partially increased.

• Fisheries and Aquatic Sciences
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• v.15 no.3
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• pp.233-239
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• 2012

Hematological analysis can provide crucial information for monitoring the health of fish. However, there is no current information available regarding hematological changes in olive flounder following infection by Vibrio scophthalmi. In this study, hematological and biochemical alterations were determined in olive flounder infected by the high virulence strain (HVS) and low virulence strain (LVS) of V. scophthalmi. Survival in serum, skin mucus, and macrophages of olive flounder was also compared between the HVS and LVS. The results demonstrated that the hematocrit value in infected fish declined from 23.4% at 0 h to 18.0% at 168 h post infection. The total protein concentration in fish infected with the HVS was significantly higher than in fish infected with the LVS and a non-infected control. Lysozyme activity was significantly different between infected and control fish. The HVS survived in serum and cell numbers increased substantially, while cell numbers of the LVS in serum decreased. These changes in hematological characteristics in fish infected by V. scophthalmi can be used as an effective and sensitive index to monitor the physiological and pathological conditions of fish. The survival and reproduction of V. scophthalmi in host serum, skin mucus, and macrophages play a major role in systemic infection and can serve as a virulence indicator for different strains.

• Journal of the Korean Society of Combustion
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• v.16 no.2
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• pp.23-32
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• 2011

To investigate cell formation in hydrocarbon/hydrogen/carbon monoxide-air premixed flames, the outward propagation and cellular instabilities were experimentally studied in a constant pressure combustion chamber at room temperature and elevated pressures. Unstretched laminar burning velocities and Markstein lengths of the mixtures were obtained by analyzing high-speed schlieren images. In this study, hydrodynamic and diffusional- thermal instabilities were evaluated to examine their effects on flame instabilities. The experimentally-measured unstretched laminar burning velocities were compared to numerical predictions using the PREMIX code. Effective Lewis numbers of premixed flames with methane addition decreased for all of the cases; meanwhile, effective Lewis numbers with propane addition increased for lean and stoichiometric conditions and increased for rich and stoichiometric cases for hydrogen-enriched flames. With the addition of propane, the propensity for cell formation significantly was diminished, whereas cellular instabilities for hydrogen-enriched flames were promoted. However, similar behavior of cellularity was obtained with the addition of methane to the reactant mixtures.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.2
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• pp.434-440
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• 2005

This study was conducted to find out the effect of Bojungikgitanggamibang(BIT) on growth, learning and memory of rats. The effects of BIT on learning and memory performance were examined in normal or memory impaired mice by using water maze task. Memory was impaired by 192 saporin. Body weight and growth of bone and tail of sample group were not significant compared with those of control groups. Acquisition test of water maze revealed that acqusitive ability of sample group significantly improved on 4,5th day compared with control group, while retentive ability of sample group was not significant. ChAT cell numbers of medial septum of sample group was significant compared with control group, and so was those of CA1, CA2 parts of hippocampus. On ChAT cell numbers of hippocampus, in CA2 part. These results suggest that BIT has an improving effect on the impaired learning through the effects on memory registration and retrieval.

• Journal of the Korea Furniture Society
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• v.29 no.1
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• pp.1-7
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• 2018

A study was carried out to observe the 1% aqueous safranine solution flow speed in longitudinal and radial directions of softwood Metasequoia glyptostroboides, diffuse-porous wood Anthocephalus cadamba and ring-porouswood Fraxinus rhynchophylla. In radial direction, ray cells and in longitudinal direction, tracheids, vessel and wood fiber were considered for the measurement of liquid penetration speed at less than 12% moisture contents (MC). The length, lumen diameter, pit diameter, end wall pit diameter and the numbers of end wall pits determined for the flow rate. The liquid flow in the those cells was captured via video and the capillary flow rate in the ones were measured. Vessel in hardwood species and tracheids in softwood was found to facilitate prime role in longitudinal penetration. Anatomical features like the length and diameter, end-wall pit numbers of ray parenchyma were found also responsible fluid flow differences. On the other hand, vessel and fiber structure affected the longitudinal flow of liquids. Therefore, the average liquid penetration depth in longitudinal tracheids of Metasequoia glyptostroboides was found the highest among all cells considered in Anthocephalus cadamba and Fraxinus rhynchophylla In radial direction, ray parenchyma of Metasequoia glyptostroboides was found the highest depth and the one of Fraxinus rhynchophylla was the lowest. The solution was penetrated lowest depth in the wood fiber of Fraxinus rhynchophylla. The large vessel of Fraxinus rhynchophylla was found the lowest depth among the vessels. The solutin was penetrated to the wood fiber of Anthocephalus cadamba higher than the one of Fraxinus rhynchophylla.

• Journal of the Korea Furniture Society
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• v.28 no.1
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• pp.88-93
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• 2017

A study was carried out to observe the 1% aqueous safranine solution flow speed in longitudinal and radial directions of softwood Larix kaempferi (Lamb.)Carriere, diffuse-porous wood Betula davurica Pall.. and ring-porouswood Castanea crenata S.etZ. In radial direction, ray cells and in longitudinal direction, tracheids, vessel and wood fiber were considered for the measurement of liquid penetration speed at less than 12% moisture contents (MC). The length, lumen diameter, pit diameter, end wall pit diameter and the numbers of end wall pits determined for the flow rate. The liquid flow in the those cells was captured via video and the capillary flow rate in the ones were measured. Vessel in hardwood species and tracheids in softwood was found to facilitate prime role in longitudinal penetration. Anatomical features like the length and diameter, end-wall pit numbers of ray parenchyma were found also responsible fluid flow differences. On the other hand, vessel and fiber structure affected the longitudinal flow of liquids. Therefore, the average liquid penetration depth in longitudinal tracheids of Larix kaempferi was found the highest among all cells considered in Betula davurica and Castanea crenata, In radial direction, ray parenchyma of Larix kaempferi was found the highest depth and the one of Betula davurica was the lowest. The solution was penetrated lowest depth in the wood fiber of Castanea crenata. The large vessel of Castanea crenata was found the lowest depth among the vessels. The solutin was penetrated to the wood fiber of Betula davurica higher than the one of Castanea crenata.