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Clinical, Hematological, and Biochemical Alterations in Olive Flounder Paralichthys olivaceus Following Experimental Infection by Vibrio scophthalmi

  • Qiao, Guo (Department of Aquaculture, National Fisheries Research & Development Institute) ;
  • Park, Soo Il (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Xu, De-Hai (US Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit)
  • Received : 2012.07.06
  • Accepted : 2012.08.21
  • Published : 2012.09.30

Abstract

Hematological analysis can provide crucial information for monitoring the health of fish. However, there is no current information available regarding hematological changes in olive flounder following infection by Vibrio scophthalmi. In this study, hematological and biochemical alterations were determined in olive flounder infected by the high virulence strain (HVS) and low virulence strain (LVS) of V. scophthalmi. Survival in serum, skin mucus, and macrophages of olive flounder was also compared between the HVS and LVS. The results demonstrated that the hematocrit value in infected fish declined from 23.4% at 0 h to 18.0% at 168 h post infection. The total protein concentration in fish infected with the HVS was significantly higher than in fish infected with the LVS and a non-infected control. Lysozyme activity was significantly different between infected and control fish. The HVS survived in serum and cell numbers increased substantially, while cell numbers of the LVS in serum decreased. These changes in hematological characteristics in fish infected by V. scophthalmi can be used as an effective and sensitive index to monitor the physiological and pathological conditions of fish. The survival and reproduction of V. scophthalmi in host serum, skin mucus, and macrophages play a major role in systemic infection and can serve as a virulence indicator for different strains.

Keywords

References

  1. Aldrin JF, Messager JL and Laurencin FB. 1982. La biochimie clinique en aquaculture. Interet et perspective. CNEXO Actes Colloq 14, 291-326.
  2. Alexander JB and Ingram GA. 1992. Non-cellular and non-specific defense mechanisms of fish. Annu Rev Fish Dis 2, 249-279. https://doi.org/10.1016/0959-8030(92)90066-7
  3. Blaxhall PC. 1972. The haematological assessment of the health of freshwater fish. J Fish Biol 4, 593-604. https://doi.org/10.1111/j.1095-8649.1972.tb05704.x
  4. Booth NJ, Elkamel A and Thune RL. 2006. Intracellular replication of Edwardsiella ictaluri in channel catfish macrophages. J Aquat Anim Health 18, 101-108. https://doi.org/10.1577/H05-025.1
  5. Bordas MA, Balebona MC, Zorrilla I, Borrego JJ and Morinigo MA. 1996. Kinetics of adhesion of selected fish-pathogenic Vibrio strains of skin mucus of gilt-head sea bream Sparus aurata L. Appl Environ Microbiol 62, 3650-3654.
  6. Clark Z. 2003. Diabetes mellitus in a 6-month-old Charolais heifer calf. Can Vet J 44, 921-922.
  7. Ellis AE. 2001. The immunology of teleosts. In: Fish Pathology. 3rd ed. Robert RJ, ed. W.B. Saunders, London, GB, pp.133-150.
  8. Ghanem MM and Abdel-Hamid OM. 2010. Clinical, haematological and biochemical alterations in heat intolerance (panting) syndrome in Egyptian cattle following natural foot-and-mouth disease (FMD). Trop Anim Health Prod 42, 1167-1173. https://doi.org/10.1007/s11250-010-9543-0
  9. Ghiraldelli L, Martins ML, Yamashita MM and Jeronimo GT. 2006. Ectoparasites influence on the haematological parameters of Nile tilapia and carp cultured in the State of Santa Catarina, South Brazil. J Fish Aquat Sci 1, 270-276. https://doi.org/10.3923/jfas.2006.270.276
  10. Han HJ, Kim DH, Lee DC, Kim SM and Park SI. 2006. Pathogenicity of Edwardsiella tarda to olive flounder Paralichthys olivaceus (Temminck & Schlegel). J Fish Dis 29, 601-609. https://doi.org/10.1111/j.1365-2761.2006.00754.x
  11. Hrubec TC, Cardinale JL and Smith SA. 2000. Hematology and plasma chemistry reference intervals for cultured tilapia Oreochromis hybrid. Vet Clin Pathol 29, 7-12. https://doi.org/10.1111/j.1939-165X.2000.tb00389.x
  12. Hudson L and Hay FC. 1989. Practical Immunology. Blackwell, London, GB, p. 507.
  13. Jamalzadeh HR, Keyvan A, Ghomi MR and Gherardi F. 2009. Comparison of blood indices in healthy and fungal infected Caspian salmon Salmo trutta caspius. Afr J Biotechnol 8, 319-322.
  14. Jo MR, Kim MC and Song CB. 2006. Development of a rapid diagnosis kit for Vibrios associated with the farmed olive flounder Paralichthys olivaceus in Jeju Island. J Fish Pathol F3.
  15. Kori-Siakpere O, Ake JEG and Idoge E. 2005. Haematological characteristics of the African snake head Parachanna obscura. Afr J Biotechnol 4, 527-530.
  16. Lee DC. 2005. Effect of Edwardsiella tarda ECPs on immune function in olive flounder Paralichthys olivaceus. Ph.D. Dissertation, Pukyong National University, Busan, KR.
  17. Leung KY, Yeap IV, Lam TJ and Sin YM. 1994. Serum resistance as a good indicator for virulence in Aeromonas hydrophila strains isolated from diseased fish in south-east Asia. J Fish Dis 18, 511-518.
  18. Martins ML, Mourino JLP, Amaral GV, Vieira FN, Dotta G, Jatoba AMB, Pedrotti FS, Jeronimo GT, Buglione-Neto CC and Pereira- Jr G. 2008. Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. Braz J Biol 68, 657-661. https://doi.org/10.1590/S1519-69842008000300025
  19. Nieto TP, Toranzo AE and Barja JL. 1984. Comparison between the bacterial flora associated with fingerling rainbow trout cultured in two different hatcheries in the north-west of Spain. Aquaculture 42, 193-206. https://doi.org/10.1016/0044-8486(84)90100-5
  20. Pruzanski W, Leers WD and Wardlaw AC. 1973. Bactericidal and bacteriolytic activity of leukemic sera. Cancer Res 33, 2048-2053.
  21. Qiao G. 2011. Microbiological and pathogenic characteristics of Vibrio scophthalmi isolated from olive flounder Paralichthys olivaceus. Ph.D. Dissertation, Pukyong National University, Busan, KR.
  22. Qiao G, Lee DC, Woo SH, Li H, Xu DH and Park SI. 2012a. Microbiological characteristics of Vibrio scophthalmi isolates from diseased olive flounder Paralichthys olivaceus. Fish Sci 78, 853-863. https://doi.org/10.1007/s12562-012-0502-8
  23. Qiao G, Li H, Xu DH and Park SI. 2012b. Modified a colony forming unit microbial adherence to hydrocarbons assay and evaluated cell surface hydrophobicity and biofilm production of Vibrio scophthalmi. J Bacteriol Parasitol 3, 130.
  24. Ram Bhaskar B and Srinivasa Rao K. 1989. Influence of environmental variables on haematology and compendium of normal haematological ranges of milkfish, Chanos chanos (Forskal), in brackishwater culture. Aquaculture 83, 123-136. https://doi.org/10.1016/0044-8486(89)90066-5
  25. Robertsen B, Rostad G, Engstad R and Raa J. 1990. Enhancement of non-specific disease resistance in Atlantic salmon Salmo salar L. by a glucan from Saccharomyces cerevisiae cell walls. J Fish Dis 13, 391-400. https://doi.org/10.1111/j.1365-2761.1990.tb00798.x
  26. Sankaran K and Gurnani S. 1972. On the variation in the catalytic activity of lysozyme in fishes. Indian J Biochem Biophys 9, 162-165.
  27. Secombes CJ. 1990. Isolation of salmonid macrophages and analysis of their killing activity. In: Techniques in Fish Immunology. Stolen JS, Fletcher TC, Anderson DP, Robertson BS and van Muiswinkel WB, eds. SOS Publications, Fair Haven, NJ, US, pp.137-154.
  28. Wang YG, Zhang Z and Qing L. 2004. The main diseases of cultured turbot Scophthalmus maximus and their prevention and treatment. Mar Fish Res 25, 61-68.
  29. Wiklund T and Dalsgaard I. 2002. Survival of Flavobacterium psychrophilum in rainbow trout Oncorhynchus mykiss serum in vitro. Fish Shellfish Immunol 12, 141-153. https://doi.org/10.1006/fsim.2001.0360
  30. Won KM and Park SI. 2008. Pathogenicity of Vibrio harveyi to cultured marine fishes in Korea. Aquaculture 285, 8-13. https://doi.org/10.1016/j.aquaculture.2008.08.013

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