• 산업경영시스템학회지
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• 제17권32호
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• pp.177-186
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• 1994

Economic Lot size decision is studied on this thesis foe the muti-product small batch production system. Even though economic lot size decision has been studied for the MRP system. this could be applied at the industry under the multi-product small batch production system because of very complicate and manager's lack of understand. Therefore, this technique is applied at the industry in order to minimize ordering cosy based on optimal quantity and period, and holding cost according to optimize inventory level under the muti-product small batch production system. After that, lot size decision technique is compared with lot size decision technique which has been used for analyzing and emphasizing productivity

• 한국식물병리학회지
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• 제12권3호
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• pp.302-306
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• 1996

항균물질을 생산하는 방선균 KGT-37를 인삼밭 토양에서 분리하여 배양액으로부터 항균물질을 분리하고 silica gel column chromatography, preparative TLC, HPLC로 정제하였다. \ulcornerH-\ulcornerH COSY, HMQC, HMBC등 NMR 분석결과 KGT-37이 생산하는 항균물질은 cyclic depsipeptide계의 항생물질인 echinomycin으로 동정되었으며 이 연구에서 지금까지 assign되지 않았던 \ulcornerH과\ulcornerC의 NMR signal이 완전히 assign되었다. 이 항균활성물질은 감자더뎅이병균인 Streptomyces scabies과 Corynebacterium lilium을 포함하는 그람 양성 세균에 특히 강한 항균활성을 보였으며, 도열병균 및 점무늬낙엽병균 등 병원곰팡이에도 항균활성을 나타내었다.

• Natural Product Sciences
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• 제19권3호
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• pp.269-274
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• 2013

Nine terpenoids, spinasterone (1), simiarenol (2), phytol (3), lupeol (4), ${\alpha}$-amyrin (5), $1{\beta},4{\beta}$-dihydroxyeudesman-11-ene (6), 3,7-dihydroxyhumula-4,8(15),10(E)-triene (7), 2,6-dihydroxyhumula-3(12),7(13), 9E-triene (8), 23-hydroxybetulin (9) were isolated from the aerial parts of Aster yomena M. Their structures were identified based on 1D and 2D NMR, including $^1H-^1H$ COSY, HSQC, HMBC and NOESY spectroscopic analyses. Compounds 1 - 9 were isolated from this plant for the first time.

• Natural Product Sciences
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• 제26권2호
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• pp.176-181
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• 2020

Two new compounds, 3-methyl-but-2-en-1-yl-1-O-β-xylopyranosyl-(1"→2')-O-β-glucopyranoside (1) and 1-O-β-glucopyranosyl-6-hydroxy-2-methyl-hep-2-enoic acid (2), along with sixteen known terpenoids were isolated from the peels of Citrus unshiu Markov. Their structures were elucidated based on extensive NMR analyses (¹H NMR, ¹³C NMR, DEPT, COSY, HMQC, and HMBC) and high-resolution mass spectrometry. In addition, all isolates (1 - 18) were tested their effects on nitric oxide (NO) production in RAW264.7 cells. Limonin (15) showed to inhibit LPS-induced NO production in a concentration-dependent manner without cytotoxicity.

• 한국양식학회지
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• 제15권3호
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• pp.139-143
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• 2002

해면동물 P. incrustans 시료를 거제도 해금강 연안에서 SCUBA 다이빙에 의하여 채집하였으며, 채집한 시료를 메탄올로 반복해서 추출한 후에 다시 dichloromethane으로 반복 추출하였다 얻어진 유기 조추출물을 n-butanol과 물을 이용하여 분배한 후 n-butanol 층은 다시 15% 메탄올 수용액층과 n-hexane으로 분배하였다. n-Hexane 분배 분획에 대한 silica flash chromatography와 이렇게 얻어진 크로마토그래피 분획에 대한 반복적인 HPLC에 의해서 saringosterol을 분리할 수 있었으며, 이차원적 핵자기 공명 분광기법에 의하여 이 화합물을 구성하는 탄소원자들의 위치를 정확히 지정할 수 있었다. 이 스테로이드는 아직까지 우리나라에서 채집한 해양생물에서는 분리된 적이 없으며 이 화합물의 탄소 위치에 대한 정확한 지정도 처음으로 이루어졌다.

• Ocean and Polar Research
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• 제38권4호
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• pp.287-294
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• 2016

Marine sponges have been a remarkably rich source of pharmacologically active and structurally diverse natural products. As a part of our continuing search for novel secondary metabolites of biomedical importance from marine invertebrate, we encountered the sponge Lipastrotethya sp. from Chuuk, Micronesia. The crude organic extract of this animal exhibited considerable cytotoxicity against the K562 cell line. Guided by the $^1H$ NMR analysis, flash chromatography of the crude extract followed by HPLC yielded a new triterpene glycoside, along with ten known saponins of the sarasinoside class. The structure of this new compound was determined by combined spectroscopic methods such as COSY, HSQC and HMBC experiment. Among these metabolites, six compounds exhibited moderate cytotoxicity against ACHN, MDA-MB-231, NCI-H23 and NUGC-3 cell lines.

• Ocean and Polar Research
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• 제39권2호
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• pp.115-124
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• 2017

The objective of this study is to evaluate the antioxidant activity of the fruits of Ligustrum japonicum. The crude extract was successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by means of solvent polarity. The crude extract and its solvent fractions were evaluated for their antioxidant effect by four different assay systems: scavenging power on peroxynitrite and intralcellular ROS produced in HT-1080 cells; DNA oxidation inhibition; ferric reducing antioxidant power (FRAP). The n-BuOH fraction exhibiting potent antioxidant activity was further purified by C18 silica gel column chromatography and RP-HPLC to give tyrosol (1) and salidroside (2). The structure of isolated compounds was determined by extensive 2 D NMR experiments such as $^1H$ COSY, NOESY, HSQC and HMBC as well as by comparison with the published spectral data.

• Natural Product Sciences
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• 제24권1호
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• pp.54-58
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• 2018

A phytochemical investigation of Allium macrostemon Bunge (Liliaceae) afforded the new pregnane steroidal glycoside, named allimacroside F (1), along with three known glycosides, benzyl-O-${\alpha}-{\text\tiny{L}}$-rhamnopyranosyl-($1{\rightarrow}6$)-${\beta}-{\text\tiny{D}}$-glucopyranoside (2), phenylethyl-O-${\alpha}-{\text\tiny{L}}$-rhamnopyranosyl-($1{\rightarrow}6$)-${\beta}-{\text\tiny{D}}$-glucopyranoside (3), (Z)-3-hexenyl-O-${\alpha}-{\text\tiny{L}}$-rhamnopyranosyl-($1{\rightarrow}6$)-${\beta}-{\text\tiny{D}}$-glucopyranoside (4). The identification and structural elucidation of a new compound (1) was carried out based on spectral data analyses ($^1H-NMR$, $^{13}C-NMR$, $^1H-^1H$ COSY, HSQC, HMBC, and NOESY) and HR-FAB-MS.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2003년도 제23차 추계총회 및 국제학술심포지움
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• pp.134.2-135
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• 2003

지초(Lithospermum efthrorhizon)뿌리는 예로부터 한방약제로 이용되어져 왔으며, 지초 뿌리에 함유된 naphtoquinon계 색소물질인 shikonin은 항암, 항균, 항바이러스, 항염증 등의 효과가 있다고 보고되었다. 그러나 지초뿌리에 함유된 기능성 물질에 관한 연구는 미비한 실정이다. 이에 본 연구에서는 지초뿌리에서 기능성 물질의 탐색 및 기능성 해명연구를 위하여 지초뿌리를 ethanol로 추출한 후 이 추출물을 n-hexane, EtOAc, MeOH로 순차적으로 추출하여 MeOH추출물을 얻었다. 이 MeOH추출물을 silica gel adsorption column chromatography, ODS column chromatography, Sephadex LH-20 column chromatography로 순차 정제한 후 Shodex Asahipak column을 이용한 GPC-HPLC에 의해 활성 물질을 분리하였다. 분리된 물질들은 1H-NMR, 13C-NMR, COSY, HSQC, HMBC, FAB-MS 등의 기기분석을 통해 당 관련 화합물인 것으로 판명되었다.

• Archives of Pharmacal Research
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• 제19권5호
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• pp.406-410
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• 1996

The flowers of Chrysanthemum boreale afforded handelin, a unique guaianolide dimer and a mixture of n-hydrocarbons and n-hydrocarbon alcohols in addition to b-sitosterol and b-sitosterol glucoside. Detailed analysis of the $^{1}H$- and $^{13}C$-NMR spectra of handelin was carried out by the application of two-dimensional $^{1}H-^{1}H$-COSY and $^{1}H$- $^{13}C$ multiple-bond, multiple-quantum spectroscopic correlation techniques. Handelin was inactive in the in vitro anti-tumor activity.