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Studies of Xanthium strumarium Extract Suppressing Th17-cell Differentiation and Anti-dermatitic Effect in BMAC-induced Atopy Dermatitis of NC/Nga Mice (창이자 추출물이 아토피 피부염 유발 생쥐의 비장 세포 Th17의 세포분화 억제에 따른 아토피 피부 상태에 미치는 영향)

  • Kim, Kum-Lan;Choe, Tae-Boo
    • KSBB Journal
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    • v.24 no.4
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    • pp.383-392
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    • 2009
  • Xanthii fructus which is well known as "Chang-ihjah" in Korea is the dried fruit of Xanthium strumarium L. (or Xanthium sibiricum PATR. Ex WIDD., Asteraceae. XS). Water extract of this fruit has been used for treatment of various inflammatory diseases such as tympanitis, allergic rhinitis, or ozena as alternative therapy material usually by oral administration in far Eastern countries including Korea. In this study, the effect of XS extract (XS-E) or XS-30% acetone fraction layer (XS-30% AFL) on the differentiation of $CD4^+$ T cells isolated from NC/Nga mouse and the production of IL-17 was investigated. The experimental results showed that $100\;{\mu}g$/mL of XS-E could decrease the production of IL-17 by $CD4^+$ Th17 cells by 2 fold and only $20\;{\mu}g$/mL of XS-30% AFL could inhibit 3.5 fold. The amount of IL-17A and IL-22 mRNA determined by real-time PCR was decreased remarkably when XS-E or XS-30% AFL was treated on $CD4^+$ Th17 cells(p<0.01, p<0.001). The amount of IL-17A protein determined by ELISA was also decreased remarkably(p<0.05, p<0.001). To study the effect of XS-E or XS-30% AFL on the proliferation of Th17 cells, $CD4^+$ T cells of a NC/Nga mouse was firstly differentiated by rIL-6/TGF-$\beta$ and then stimulated by rIL-23. The control group of Th17 cells were doubled every each day, while those of XS-E or XS-30% AFL treated group were shown to be delayed remarkably by these extracts. In conclusion, XS can inhibit the differentiation of Th17 cells of NC/Nga mouse and the production of IL-17 successfully, which may be a beneficial result for the treatment of atopic skin dermatitis.

Mitigation of Carbon Dioxide and Heavy Metals by Urban Greenspace (도시녹지의 이산화탄소 및 중금속 저감)

  • Park, Joo-Young;Ju, Jin-Hee;Yoon, Yong-Han
    • Journal of Environmental Policy
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    • v.9 no.1
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    • pp.137-154
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    • 2010
  • These objectives of this study were to compute heavy metal and accumulation carbon dioxide fixing quantity from urban green space(street trees and urban parks) in Cheong-ju city and Chungju-city and thus to estimate the effect of urban green space for improving the urban environment. The results are summarized below. 1. Results of the total accumulation of the carbon dioxide fixing quantity of street trees, Cheong-ju city and Chungju-city street tree was 1, 230,000kg-C, 1,270,000 kg-C, respectively. Total accumulation carbon dioxide fixing quantity of Balssan urban park had a 25,000kg-C in Cheong-ju city, Degami sports park had a 6,400kg-C in Chungju-city. 2. Results of heavy metal for street trees, fell in the order Zn > Cu > Cr > Ni >, the highest accumulated heavy metal was Zn, and the lowest was Ni. Total heavy metal concentration according to land-use area, was observed in order, for residental areas(157.26 mg/kg) > industrial areas(141.71 mg/kg) > commercial areas(118.55 mg/kg) > and greenspace areas(61.95 mg/kg) in Cheong-ju city. 3. Total heavy metal concentration for street trees fell in the order of commercial areas(84.48 mg/kg) > residental areas(83.70 mg/kg) > and greenspace(48.23 mg/kg) according to land-use area in Chungju-city. Comparatively, Cheong-ju city had more total heavy metal concentration than Chungju-city. 4. Heavy metal for soil that planted street trees was observed in order of Zn > Cu > Pb( > Ni > Cr > As > Cd), and Zn was highest, and Cd was lowest. Total heavy metal concentration for soil fell in the order commercial area(91.82mg/kg) > industrial area(85.96mg/kg) > residental area(67.55mg/kg) > greenspace(43.13mg/kg) according to land-use area in Cheong-ju city. 5. Heavy metal for soil that planted street trees was observed in order of Zn > Pb > Cu( > Ni > Cr > As > Cd, and Zn was highest. Total heavy metal concentration for soil fell in the order commercial area(87.66mg/kg) > greenspace(72.73mg/kg) $${\geq_-}$$ residental area(70.10mg/kg) in Chungju-city.

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Quantification of Thermal Insulation by Clothing Items and Analysis of Influencing Factors (단일의복의 보온력 정량화와 영향 요인)

  • Baek, Yoon Jeong;Hwang, Soo Kyung;Lee, Hyo Hyun;Park, Joonhee;Kim, Do-Hee;Lee, Joo-Young
    • Journal of the Korean Society of Clothing and Textiles
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    • v.42 no.1
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    • pp.172-182
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    • 2018
  • The purpose of the present study was to quantify the thermal insulation of garments by item and examine factors influencing clothing insulation. A total of 769 garments in clo unit were collected and classified into 12 categories: blouses/shirts (95 items, BS), T-shirts/sweaters (62 items, TS), vest (23 items, VT), cardigans (23 items, CD), jackets/coats (75 items, JC), sport outerwear (including padding jackets)(48 items, SO), trousers (23 items, TR), skirts (56 items, SK), dresses (28 items, DS), underwear (150 items, UW), sleepwear (50 items, SW), and personal protective clothing (59 items, PPC). The results showed that clothing insulation was $0.21{\pm}0.01clo$ for the BS, $0.22{\pm}0.01clo$ for TS, $0.12{\pm}0.00clo$ for VT, $0.23{\pm}0.02clo$ for CD, $0.40{\pm}0.02clo$ for JC, $0.49{\pm}0.03clo$ for SO, $0.21{\pm}0.01clo$ for TR, $0.18{\pm}0.01clo$ for SK, $0.34{\pm}0.03clo$ for DS, $0.09{\pm}0.01clo$ for UW, $0.42{\pm}0.03clo$ for SW, and $0.56{\pm}0.03clo$ for PPC (p<.001). The most influential factors among the seven factors for thermal insulation of garments were clothing weight and covering area; however, the explanatory powers of two factors differed according to clothing categories. The covering area had more significant impact on clothing insulation in cardigans, jackets/coats, trousers, and dresses than clothing weight. Covering areas and clothing weight were the most influential factors in the following categories: blouses/shirt, T-shirts/sweaters, skirts, sleepwear and personal protective clothing. The garment weight was the most important factor for thermal insulation for the sport outerwear.

Etiologic and Immunologic Characteristics of Thoroughbred Horses with Bacterial Infectious Upper Respiratory Disease at the Seoul Race Park

  • Ryu, Seung-Ho;Koo, Hye-Cheong;Park, Young-Kyung;Kim, Jun-Man;Jung, Woo-Kyung;Davis, William C.;Park, Yong-Ho;Lee, Chang-Woo
    • Journal of Microbiology and Biotechnology
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    • v.19 no.9
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    • pp.1041-1050
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    • 2009
  • Equine respiratory disease is a common cause of poor performance and training interruptions. The higher incidence rate of infectious upper respiratory disease (IURD) in thoroughbred racehorses at the Seoul Race Park coincided with the frequent stabling season, shorter stabling periods, and younger ages in this study. Incidence rates were also correlated with significantly lower proportions of cells expressing MHC class II-, CD2 antigen-, $CD4^+$- or $CD8^+$-T lymphocyte-, and B lymphocyte in IURD patients compared with healthy control groups in the summer and fall and in 2-and-3-year-old groups. The data suggested that movement and new environments may have resulted in immunosuppression and inappropriate responses to respiratory pathogens in IURD patients. The IURD incidence decreased with age, perhaps by the acquisition of immunity, and study results suggested that immunologic protection was associated with IURD, particularly in young thoroughbred racehorses. Streptococci isolates were identified in 11 of 72 IURD horses, and 3 of these isolates were identified as Streptococcus. equi subsp. equi. S. equi subsp. zooepidemicus was isolated from 2 of 23 IURD horses in the spring (8.7%), 5 of 23 in the summer (21.7%), and 1 of 6 in winter (16.7%). S. equi subsp. zooepidemicus (5%) was also identified in 3 of 61 isolates from clinically normal horses. Racetracks should implement anti-IURD protective measures by assessing the capacity of equine immunologic protection at the Park and by limiting the introduction of specific respiratory pathogens (such as S. equi subsp. equi) by preventing the access of infected but subclinical horses with a specified respiratory pathogen-free certification system prior to Park entry.

Determination of bromate in bottled mineral water in Korea (먹는 샘물 중 bromate 분석 및 분포 특성)

  • Ahn, Hye-Sil;Kim, Hyun-Koo;Park, Ju-Hyun;Jung, Dong-Il;Kim, Tae-Seung
    • Analytical Science and Technology
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    • v.23 no.2
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    • pp.155-160
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    • 2010
  • This study was performed to compare international analytical methods of bromate and applied to determine bromate in bottled mineral water in Korea. Bromate in bottled mineral water was eluted by 10~50 mM potassium hydroxide (KOH) and determined by ion chromatograph-suppressed conductivity detector (ICCD). Sample was purified with on guard Ba-, Ag- and H-cartridges and $0.2\;{\mu}m$ membrane filter. The method detection limit (MDL) and the limit of quantitation (LOQ) of bromate were 0.1 and $0.5\;{\mu}g/L$, respectively. The calibration curve showed good lineality above 0.9998 in the ranges of the $0.5\sim40\;{\mu}g/L$. Bromate from 33 samples among total 157 bottled mineral water samples was detected in the concentration range of $0.5\sim6.5\;{\mu}g/L$. The detected concentrations were within bottled mineral water quality criteria ($10\;{\mu}g/L$) in Korea.

Spatial Distribution Patterns of a Newly Invaded Honeybee Pest, Aethina tumida Murray, 1867 (Coleoptera: Nitidulidae) in an Apiary Where it was First Detected (꿀벌 외래 해충, 작은벌집밑빠진벌레(Aethina tumida Murray, 1867)의 초기 발견 봉장 내 공간 분포 특성)

  • Hong, Seokmin;Jung, Chuleui
    • Journal of Apiculture
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    • v.32 no.3
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    • pp.163-170
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    • 2017
  • Small hive beetle (SHB, Aethina tumida Murray (Coleoptera: Nitidulidae) is a honeybee pest infesting combs and stores inside the hive. Contamination of the SHB on Apis mellifera colonies were firstly noticed on September 23, 2016, in Miryang City, Gyeongnam Province in Korea. After that, on October 5, 2016, we investigated the spatial distribution of SHB inside the hive and outside soil within the apiary. Total of 169 beehives were observed. We found all stages of SHB inside or outside of hives. 61% of hives infested with the adult SHB still had live honeybees whereas hives containing larval SHB did not have bees, implying colony destruction In hives with live bees, infestation density was higher as bee population became higher. Coefficient of dispersions (CD) showed significant clumped distribution of infestation among hives. CDs were much higher inside hive than outside soil. Our results indicates SHB could result in honeybee colony collapse it not managed properly. Also even during October, there are new infestation into honeybee colonies and every stage of SHB could still be able to develop. Further detailed analysis of this insect' adaptation in Koran environment could help guide the management strategies of the invaded new pest of honeybee.

Utilization Evaluation of Numerical forest Soil Map to Predict the Weather in Upland Crops (밭작물 농업기상을 위한 수치형 산림입지토양도 활용성 평가)

  • Kang, Dayoung;Hwang, Yeongeun;Yoon, Sanghoo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.23 no.1
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    • pp.34-45
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    • 2021
  • Weather is one of the important factors in the agricultural industry as it affects the price, production, and quality of crops. Upland crops are directly exposed to the natural environment because they are mainly grown in mountainous areas. Therefore, it is necessary to provide accurate weather for upland crops. This study examined the effectiveness of 12 forest soil factors to interpolate the weather in mountainous areas. The daily temperature and precipitation were collected by the Korea Meteorological Administration between January 2009 and December 2018. The Generalized Additive Model (GAM), Kriging, and Random Forest (RF) were considered to interpolate. For evaluating the interpolation performance, automatic weather stations were used as training data and automated synoptic observing systems were used as test data for cross-validation. Unfortunately, the forest soil factors were not significant to interpolate the weather in the mountainous areas. GAM with only geography aspects showed that it can interpolate well in terms of root mean squared error and mean absolute error. The significance of the factors was tested at the 5% significance level in GAM, and the climate zone code (CLZN_CD) and soil water code B (SIBFLR_LAR) were identified as relatively important factors. It has shown that CLZN_CD could help to interpolate the daily average and minimum daily temperature for upland crops.

Evaluation of Probiotics as an Alternative to Antibiotic on Growth Performance, Nutrient Digestibility, Occurrence of Diarrhea and Immune Response in Weaning Pigs (항생제 대체제로서 생균제가 이유자돈의 성장능력 및 영양소 이용률, 설사 빈도, 면역 반응에 미치는 영향)

  • Jang, Young-Dal;Oh, Hee-Kyung;Piao, Long-Guo;Choi, Hyun-Bong;Yun, Jin-Hyeon;Kim, Yoo-Yong
    • Journal of Animal Science and Technology
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    • v.51 no.1
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    • pp.25-32
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    • 2009
  • This study was conducted to determine the effects of probiotics supplementation on growth performance, nutrient digestibility, occurrence of diarrhea and immune response in weaning pigs. Treatments were 1) NC (basal diet), 2) PC (basal diet + 0.12% avilamycin) and 3) A (basal diet + 0.2% Aspergillus oryzae), 4) B (basal diet + 0.2% Lactobacillus casei), 5) C (basal diet + 0.2% Bacillus subtilis), 6) D (basal diet + 0.2% Lactobacillus crispatus). A total of 120 pigs ($7.16\pm0.01$ kg average body weight, weaned at $23\pm3$days of age) were assigned to 6 treatments, 5 replicates and 4 pigs per pen in a randomized complete block (RCB) design. During the whole experimental period, body weight (P<0.01), average daily gain (ADG; P<0.01), and average daily feed intake (ADFI; P<0.05) of treatment PC were higher than other treatments. However, the probiotics treatments tended to increase ADG and G:F ratio compared to treatment NC. The G:F ratio in treatment A (Aspergillus oryzae) was similar to treatment PC during the whole experimental period (P<0.05). The supplementation of probiotics in the diet of weaning pig did not change nutrient digestibility (dry matter, crude protein, crude fat and ash) and nitrogen retention of weaning pigs. In blood urea nitrogen (BUN) concentration, treatment B had lower value than other treatments at 2 and 4 weeks (P<0.05). Treatments PC and C tended to decrease diarrhea score than other treatments (P=0.18). At 3h after lipopolysaccharide (LPS) injection, treatments NC and PC had higher count of $CD^{4+}$ T-cells than probiotics treatments, and treatment C showed the lowest value (P<0.01). There were no differences on count of $CD^{8+}$ T-cells and $CD^{4+}:CD^{8+}$ ratio among all treatments (P>0.10). These results suggest that the dietary probiotics are likely able to improve the growth performance, occurrence of diarrhea and immune response although they do not have similar effects like antibiotics in weaning pigs.

A Study on Migration of Heavy Metals from Kitchen Utensils Including Glassware, Ceramics, Enamel, Earthenware and Plastics (유리제 등 조리기구 중 중금속 이행에 관한 연구)

  • Choi, Jae-Chon;Park, Se-Jong;Goh, Hyeah;Lee, Ju Yeun;Eom, Mi Ok;Kim, Meehye
    • Journal of Food Hygiene and Safety
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    • v.29 no.4
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    • pp.334-339
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    • 2014
  • The purpose of our study was to investigate the migration level of lead (Pb), cadmium (Cd), antimony (Sb), arsenic (As), hexavalent chromium ($Cr^{6+}$) and mercury (Hg) from cookwares into food simulants and to evaluate the safety of each heavy metals. The test articles for heavy metals were glassware, ceramics, enamel, earthenware, polypropylene and polyethylene cookwares for Pb and Cd, enamel for Sb, earthenware for As, polyethylene and polypropylene cookwares for $Cr^{6+}$ and Hg. All the article samples of 391 intended for contact with foods were purchased in domestic markets. Pb, Cd, Sb and As were analyzed by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), $Cr^{6+}$ by UV visible spectrophotometer and Hg by mercury analyzer. The migration levels of heavy metals in all the samples were within the migration limits of Ministry of Food and Drug Safety (MFDS). As a result of safety evaluation, our results showed that the estimated daily intakes (EDI, mg/kg bw/day) were $9.12{\times}10^{-6}$ and $8.83{\times}10^{-7}$ for Pb and Cd from ceramics and $1.19{\times}10^{-5}$, $1.23{\times}10^{-5}$ and $7.52{\times}10^{-6}$ for Pb, Cd and Sb from enamel. Tolerable daily intakes (TDI, mg/kg bw/day) were established respectively as 0.0036, 0.00081, 0.0021, and 0.0006 for Pb, Cd, As and Hg by JECFA (Joint FAO/WHO Expert Committee on Food Additives), as 0.0060 for Sb by WHO (World Health Organization). When comparing with TDIs, the EDIs accounted for 0.25% and 0.11% for Pb and Cd from ceramics and 0.33%, 1.52% and 0.13% for Pb, Cd and Sb from enamel.

Distribution of Heavy metals in Soil at Iksan 2nd Industrial Complex Area (익산 제 2공단 토양의 중금속 함량 분포 조사)

  • Kim, Seong-Jo;Baek, Seung-Hwa;Moon, Kwang-Hyun;Jang, Kwang-Ho;Kim, Su-Jin;Lee, Seung-Hyeon
    • Korean Journal of Environmental Agriculture
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    • v.18 no.3
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    • pp.250-258
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    • 1999
  • The purpose of this study was to compare heavy metal concentrations in uncontaminated soil with those in soil influenced by industrial activities, and to investigate the relationship between change of heavy metal content and the kind of industry at the Iksan 2nd Industrial Complex that has started since 1995. Soils sampled in 0-3 cm and 3-6 cm soil depth, respectively were analized for content of Cd, Cu, Ni, Pb and Zn. The content of Cd in soil layer of 0 to 3 cm was 0.07-4.37ppm range, average concentration was 0.516ppm and 3-6 cm was 0.07-8.52ppm range, average concentration was 0.380ppm. Area of the chemicals, dyes and metal products manufacturing were higher than the other manufacturing area in Industrial Complex. The content of Cu in soil layer of 0 to 3 cm was 0.61-42.62ppm range, average concentration was 11.087ppm and 3-6 cm was 0.16-35.45ppm range, average concentration was 7.578ppm. Area of the metal products manufacturing were higher than the other manufacturing area in Industrial Complex. The content of Ni in soil layer of 0 to 3 cm was 0.19-15.93ppm range, average concentration was 5.525ppm and 3-6 cm was 0.39-15.59ppm range, average concentration was 5.310ppm. Area of the metal and chemical products manufacturing were higher than the other manufacturing area in Industrial Complex. The content of Pb in soil layer of 0 to 3 cm was 3.10-55.75ppm range, average concentration was 23.543ppm and 3-6 cm was 3.35-46.55ppm range, average concentration was 19.198ppm. Area of the chemicals and metal products manufacturing were higher than the other manufacturing area in Industrial Complex. The content of Zn in soil layer of 0 to 3 cm was 26.50-943.00ppm range, average concentration was 158.329ppm and 3-6 cm was 35.45-882.45ppm range, average concentration was 127.914ppm. Area of the chemicals and metal products manufacturing were higher than the other manufacturing area in Industrial Complex. As the result, this study was to compare Cd, Cu, Ni, Pb, Zn average concentration in uncontaminated soil of world with those in soil, that Cu, Ni were uncontaminated concentration level, Cd was somewhat higher compare with the concentration level of world, Pb and Zn were very higher. Soil contaminated degree of Iksan 2nd Industrial Complex was known a difference by type of industrial activities(chemical, dyes and metal of products)

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