Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Evaluation of Probiotics as an Alternative to Antibiotic on Growth Performance, Nutrient Digestibility, Occurrence of Diarrhea and Immune Response in Weaning Pigs

항생제 대체제로서 생균제가 이유자돈의 성장능력 및 영양소 이용률, 설사 빈도, 면역 반응에 미치는 영향

  • Jang, Young-Dal (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Oh, Hee-Kyung (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Piao, Long-Guo (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Hyun-Bong (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Yun, Jin-Hyeon (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Yoo-Yong (School of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University)
  • 장영달 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 오희경 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 박용국 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 최현봉 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 윤진현 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원) ;
  • 김유용 (서울대학교 농업생명과학대학 농생명공학부, 서울대학교 농업생명과학연구원)
  • Received : 2008.12.24
  • Accepted : 2009.02.19
  • Published : 2009.02.01
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Abstract

This study was conducted to determine the effects of probiotics supplementation on growth performance, nutrient digestibility, occurrence of diarrhea and immune response in weaning pigs. Treatments were 1) NC (basal diet), 2) PC (basal diet + 0.12% avilamycin) and 3) A (basal diet + 0.2% Aspergillus oryzae), 4) B (basal diet + 0.2% Lactobacillus casei), 5) C (basal diet + 0.2% Bacillus subtilis), 6) D (basal diet + 0.2% Lactobacillus crispatus). A total of 120 pigs ($7.16\pm0.01$ kg average body weight, weaned at $23\pm3$days of age) were assigned to 6 treatments, 5 replicates and 4 pigs per pen in a randomized complete block (RCB) design. During the whole experimental period, body weight (P<0.01), average daily gain (ADG; P<0.01), and average daily feed intake (ADFI; P<0.05) of treatment PC were higher than other treatments. However, the probiotics treatments tended to increase ADG and G:F ratio compared to treatment NC. The G:F ratio in treatment A (Aspergillus oryzae) was similar to treatment PC during the whole experimental period (P<0.05). The supplementation of probiotics in the diet of weaning pig did not change nutrient digestibility (dry matter, crude protein, crude fat and ash) and nitrogen retention of weaning pigs. In blood urea nitrogen (BUN) concentration, treatment B had lower value than other treatments at 2 and 4 weeks (P<0.05). Treatments PC and C tended to decrease diarrhea score than other treatments (P=0.18). At 3h after lipopolysaccharide (LPS) injection, treatments NC and PC had higher count of $CD^{4+}$ T-cells than probiotics treatments, and treatment C showed the lowest value (P<0.01). There were no differences on count of $CD^{8+}$ T-cells and $CD^{4+}:CD^{8+}$ ratio among all treatments (P>0.10). These results suggest that the dietary probiotics are likely able to improve the growth performance, occurrence of diarrhea and immune response although they do not have similar effects like antibiotics in weaning pigs.

본 실험은 이유자돈 120두를 이용하여 생균제의 첨가가 이유자돈의 성장 능력, 영양소 소화율, 혈중 요소태 질소, 설사 빈도, 면역 반응에 미치는 영향에 대해 조사하였다. 이유자돈 사료에 항생제 대체제인 생균제의 첨가는 전 사양 기간에 걸쳐 사양 성적이 항생제 첨가구(PC)에 비해 유의적으로 낮게 나타났다. 항생제 첨가구(PC)를 제외하고 비교하였을 때에는 비록 유의적인 차이는 발생하지 않았지만 생균제 첨가구가 항생제 무첨가구(NC)에 비해 일당증체량, 사료효율에서 높은 경향을 보였으며, 이는 항생제 첨가 효과에는 미치지 못하지만 항생제 대체제로서 그 가능성을 보인 것이라고 하겠다. 특히 생균제 A 첨가구는 0~5 주차 사료 효율(P<0.05)에서 항생제 첨가구(PC)와 비슷한 성적을 나타내어, 항생제 대체제로서 이유자돈의 성장을 개선 시킬 가능성이 가장 높다고 하겠다. 본 실험에서는 5주동안 이유자돈의 사료에 생균제를 첨가하여 사양성적을 보았는데, 생균제의 급여기간을 좀 더 늘리면 생균제의 처리효과가 더욱 뚜렷하게 나타날 수도 있으리라 생각된다. 이유자돈 사료에 생균제의 첨가가 건물, 조단백질, 조지방, 조회분 소화율에 어떠한 영향도 미치지 않았으며(P>0.10), 질소 축적율은 생균제 첨가구에서 항생제 첨가구(PC)에 비해 높게 나타났으나 일관된 효과를 보이지는 않았다. 혈중 요소태 질소 농도에서는 2주차와 4주차에서 유의적인 차이가 나타났다(P<0.05). 또한 생균제 B 첨가구가 다른 처리구에 비해 혈중 요소태 질소 농도가 가장 낮게 나타났다. 설사 발생 빈도 측정 결과, 항생제 첨가구(PC)에서 설사 발생 빈도가 유의적으로 낮게 나타났으며, 생균제 C 첨가구에서 다음으로 낮게 나타났다(P=0.18). 면역 반응 실험 결과, $CD^{4+}$ T-cell 수의 경우 생균제 C 첨가구에서 다른 처리구에 비해 가장 낮게 나타났으며(P<0.01), $CD^{8+}$ T-cell 수와 $CD^{4+}:CD^{8+}$ 비율의 경우에는 모든 처리구에서 유의적인 차이가 발생하지 않았다(P>0.10). 결론적으로 이유자돈사료 내 생균제의 첨가가 이유자돈의 성장 능력, 질소축적율, 혈중 요소태 질소, 설사 빈도, 및 면역 능력에 일정한 개선 효과를 갖고 있지만, 항생제 첨가 효과에는 미치지 못한다고 하겠다. 본 실험을 통해 이유자돈 사료에 생균제의 첨가는 항생제 대체제로서 일정 부분 개선효과가 있는 것으로 사료된다.

Keywords

References

  1. Adami, A. and Cavazzoni, V. 1999. Occurrence of selected bacterial groups in the faeces of piglets fed with Bacillus coagulans as probiotic. J Basic Microbiol. 39(1):3-9. https://doi.org/10.1002/(SICI)1521-4028(199903)39:1<3::AID-JOBM3>3.0.CO;2-O
  2. AOAC. 1995. Official Methods of Analysis (16th Ed.). Association of Official Analytical Chemist. Washingtons, DC, USA
  3. Bergner, H. 1977. Protein evaluation and protein metabolism. FEBS 11th Meeting. Copenhagen 1977, 149-160.
  4. Bonomi, A. 1992. Probiotics in pig breeding. Results from the use of Bacillus subtilis and Bacillus licheniformis. Experimental contribution. Rivista-Della-Societa-Italiana-Di-Scienza-Dell’ 'Alimentazione. 21(4):481-499.
  5. Breves, G., Walter, C., Burmester, M. and Schroder, B. 2000. In vitro studies on the effects of Saccharomyces boulardii and Bacillus cereus var. toyoi on nutrient transport in pig jejunum. J Anim Physiol Anim Nutr. 84(12):9-20. https://doi.org/10.1046/j.1439-0396.2000.00277.x
  6. Cai, Y. and Zimmerman, D. R. 1996. Lysine requirement of the lactating sow determined by using plasma urea nitrogen as a rapid response criterion. J. Anim Sci. 74, 1056-1062. https://doi.org/10.2527/1996.7451056x
  7. Cho, K. H., Lee, U. T., Yang, C. K., Yu, I. W., Kim, Y. S. and Yoon, Y. D. 1992. The effect of Lactobacillus casei (TSC-66) for growth promotion in piglets (Korean). Kor J Vet Pub Health. 16(1):49-53.
  8. Coma, J., Zimmerman, D. R. and Carrion, D. 1996. Lysine requirement of the lactating sow determined by using plasma urea nitrogen as a response criterion. J. Anim. Sci. 74, 1056-1062. https://doi.org/10.2527/1996.7451056x
  9. Dugas, B., Mercenier, A., Lenoir-Wijnkoop, I., Arnaud, C., Dugas, N. and Postaire, E. 1999. Immunity and probiotics. Immunol. Today 20:387-390. https://doi.org/10.1016/S0167-5699(99)01448-6
  10. Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66:365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  11. Gardiner, G., Stanton, C., Lynch, P. B., Collins, J. K., Fitzgerald, G. and Ross, R. P. 1999. Evaluation of Cheddar cheese as a food carrier for delivery of a probiotic strain to the gastrointestinal tract. J Dairy Sci. 82(7):1379-1387. https://doi.org/10.3168/jds.S0022-0302(99)75363-4
  12. Hahn, J. D., Gahl, M. J., Giesemann, M. A., Holsgraefe, D. P. and Fodge, D. W. 1995. Diet type and feed form effects on the performance of finishing swine fed the ${\beta}-mannanase$ enzyme product Hemicell. J. Anim. Sci. 73(Suppl. 1):175. (Abstr.)
  13. Hale, O. M. and Newton, G. L. 1979. Effects of a nonviable lactobacillus species fermentation product on performance of pigs. J. Anim. Sci. 48:770-775. https://doi.org/10.2527/jas1979.484770x
  14. Hatori, Y., Noguchi, G., Itoh, M. and Ishibashi, T. 1994. Effects of dietary protein levels on performance, plasma amino acid concentrations and biochemical components of female growing pigs. Anim. Sci. Tech. 65, 942-949.
  15. Havenaar, R. and Huis in’t Veld, J. 1992. Probiotics: A general view. In:wood ed. The lactic acid bacteria in health and disease. London Elsevier Applied Science, 209-224.
  16. Kornegay, E. T., Wood, C. M., Ball, G. G. and Risley, C. R. 1990. Use of lactobacillus acidophilus for growing and finishing pigs. VA Polytech. Inst. State Univ. Anim. Sci. Res. Rep. 9:13
  17. Kornegay, E. T., Rhein-Welker, D., Lindemann, M. D. and Wood, C. M. 1995. Performance and nutrient digestibility in weanling pigs as influenced by yeast culture additions to starter diets containing dried whey or one of two fiber sources. J. Anim Sci. 73:1381-1389. https://doi.org/10.2527/1995.7351381x
  18. Kyriakis, S. C., Tsiloyiannis, V. K., Vlemmas, J., Sarris, K., Tsinas, A. C., Alexopoulos, C. and Jansegers, L. 1999. The effect of probiotic LSP 122 on the control of post-weaning diarrhoea syndrome of piglets. Res. Vet. Sci. 67:223-228. https://doi.org/10.1053/rvsc.1999.0308
  19. Manner, K. and Spieler, A. 1997. Probiotics in piglets-An alternative to traditional growth promoters. Micoecol. Ther. 26:243-256.
  20. NRC. 1998. Nutrient Requirements of Swine (10th Ed.). National Academy press, Washington, D. C.
  21. Perdigon G., Roy Fuller and Raul Raya, 2001 Lactic Acid Bacteria and their Effect on the Immune System Current Issues in Intestinal Microbiology 2(1):27-42.
  22. Pollman, D. S., Danielson, D. M. and Peo, E. R. 1980. Effects of microbial feed additives on performance of starter and growing-finishing pigs. J. Anim. Sci. 51:577-581 https://doi.org/10.2527/jas1980.513577x
  23. Quintero Moreno A, Huerta Leidenz N, Parra de Solano N, Rincon Urdaneta E, Aranguren Mendez JA, De Solano NP. Effect of probiotics and sex on growth and carcass characteristics of pigs. Rev Cientif Facul De-Ciencias Vet Univers Zulia. 1996; 6(1):5-12.
  24. SAS. 2005. SAS User’s Guide: Statistics, SAS Inst, Inc., Cary, NC
  25. Tkachev, E. Z. and Gvyzin, O. L. 1995. Digestive and metabolic functions of the gastrointestinal tract of piglets. Russ Agr Sci. 4:8-11.
  26. Whang, K. Y. and Easter, R. A. 2000. Blood urea nitrogen as an index of feed efficiency and lean growth potential in growing-finishing swine. Asian-Aust. J. Anim. Sci. 13:811-816. https://doi.org/10.5713/ajas.2000.811
  27. Xuan, Z. N., Kim, J. D., Heo, K. N., Jung, H. J., Lee, J. H., Han, Y. K., Kim, Y. Y. and Han, In K. 2001. Study on the development of probiotics complex for weaned pigs. Asian-Aust. J. Anim. Sci. 14:1425-1428. https://doi.org/10.5713/ajas.2001.1425
  28. Zani, J. L. and Dacruz, F. W. 1998. Dossantos AF, Gilturnes C. Effect of probiotic CenBiot on the control of diarrhoea and feed efficiency in pigs. J Appl Microbiol. 84(1):68-71. https://doi.org/10.1046/j.1365-2672.1997.00309.x
  29. 길동용, 임종선, 전경철, 김법균, 김경수, 김유용. 2004. 지속적인 생균제의 첨가가 돼지의 성장, 영양소 이용률, 혈중 요소태 질소 및 면역 능력에 미치는 영향. 한국동물자원과학회지 46(1):39-48. https://doi.org/10.5187/JAST.2004.46.1.039
  30. 임희석, 김병한, 백인기. 2004. 사료내 $Natufermen^{\circleR}$의 첨가가 이유자돈의 생산성에 미치는 영향. 한국동물자원과학회지 46(6):981-988.

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