• Journal of Ginseng Research
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• 제45권6호
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• pp.683-694
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• 2021

Background: Ginsenoside Rg1 (Rg1) has been well documented to be effective against various cardiovascular disease. The aim of this study is to evaluate the effect of Rg1 on mechanical stress-induced cardiac injury and its possible mechanism with a focus on the calcium sensing receptor (CaSR) signaling pathway. Methods: Mechanical stress was implemented on rats through abdominal aortic constriction (AAC) procedure and on cardiomyocytes and cardiac fibroblasts by mechanical stretching with Bioflex Collagen I plates. The effects of Rg1 on cell hypertrophy, fibrosis, cardiac function, [Ca²⁺]_i, and the expression of CaSR and calcineurin (CaN) were assayed both on rat and cellular level. Results: Rg1 alleviated cardiac hypertrophy and fibrosis, and improved cardiac decompensation induced by AAC in rat myocardial tissue and cultured cardiomyocytes and cardiac fibroblasts. Importantly, Rg1 treatment inhibited CaSR expression and increase of [Ca²⁺]_i, which similar to the CaSR inhibitor NPS2143. In addition, Rg1 treatment inhibited CaN and TGF-b1 pathways activation. Mechanistic analysis showed that the CaSR agonist GdCl₃ could not further increase the [Ca²⁺]_i and CaN pathway related protein expression induced by mechanical stretching in cultured cardiomyocytes. CsA, an inhibitor of CaN, inhibited cardiac hypertrophy, cardiac fibrosis, [Ca²⁺]_i and CaN signaling but had no effect on CaSR expression. Conclusion: The activation of CaN pathway and the increase of [Ca²⁺]_i mediated by CaSR are involved in cardiac hypertrophy and fibrosis, that may be the target of cardioprotection of Rg1 against myocardial injury.

• 한국식품과학회지
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• 제48권5호
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• pp.496-501
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• 2016

본 연구는 농축 블루베리주스를 제조 시 구연산(CA) 또는 산성메타인산나트륨(ASM)을 산미료로써 이용하여 열처리과정에서 블루베리의 폴리페놀과 안토사이아닌과 같은 기능성 성분농도와 산화방지활성, elastase 억제활성 및 tyrosinase 억제활성 등의 저하를 최소화시키는데 있다. 열처리한 농축 블루베리주스의 색가는 1% CA+1% ASM 처리군이 54.672로 가장 높았고, 1% CA 처리군이 33.034, 대조군이 22.083의 순으로 나타났다. 총 안토사이아닌 함량에서도 1% CA+1% ASM 처리군이 65.747 mg/100 g, 1% CA 처리군, 대조군 순으로 각각 46.022, 30.864 mg/100 g으로 나타났다. 산화방지활성으로써 DPPH radical 소거능의 경우 대조군은 67.983%, 1% CA 처리군은 75.486%, 1% CA+1% ASM 처리군은 87.338%로 나타났다. Elastase 억제활성에서는 대조군, 1% CA, 1% CA+1% ASM 처리군의 결과 값은 각각 26.063, 26.336, 28.285%로 나타났다. Tyrosinase 억제활성에서는 대조군 26.375%, 1% CA 처리군 67.598%, 1% CA+1% ASM 처리군은 77.891% 순으로 높게 나타났다. 모든 분석 치에서 대조군과 1% CA+1% ASM 처리군 간에는 5% 내에서 유의성 차이가 있었다. 본 결과를 통해 ASM처리가 열처리과정에서 기능성성분과 생리활성의 저하를 억제하여주는 역할을 하는 것으로 나타났으며 새로운 산미료로서 활용할 수 있을 것으로 기대한다.

• Journal of Nutrition and Health
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• 제34권1호
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• pp.54-61
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• 2001

The Ca and P balance studies were carried out with two different diets varying Ca levels : the current Korean RDA level(normal Ca diet) and the high level (High Ca diet) which was determined by adding 2SD to mean Ca intake of all subjects. The subjects were sever healthy adult woman, aged from 25 to 32 years old. The metabolic studies were conducted for two weeks with a days recess in between : during the fist week with normal Ca diet and during the second week with high Ca diet. The composition of nutrients excepting Ca of both diets was similar to subjects usual intake. The result were summarized as follows: Mean daily Ca intake was 728.8mg from norma Ca diet and 945.5mg from high Ca diet. Fecal excretion of Ca increased significantly(p<0.05) on high Ca diet, but urinary excretion of Ca did not show any differences between the two diet periods. There were also no significant differences in Ca retention between the two diet periods but it tended to be greater during high Ca diet period: 112.1mg/day during normal Ca diet period vs 208.2mg/day during high Ca diet period. Mean apparent Ca absorption was 41.2% on normal Ca diet and 42.1% on high Ca diet, indicating it was not affected by high Ca intake level used in this study. On the contrary, P retention was significantly increased up to 109.4mg/day with high Ca diet as compared to- 41mg/day with normal Ca diet. There were no significant differences in fecal and urinary excretion of P but those to be lower during high Ca diet period. The above results showed that higher Ca intake more than current RDA(700mg/day), in the level of 945.5mg/day, could increase Ca retention through Ca absorption comparable to the rate appeared on RDA level intake. P retention was also improved by high Ca intake. Therefore, higher Ca intake than the current RDA level seemed to produce favorable effects on bone health in adult women. However, the current RDA level seemed to be relatively appropriate, considering the results that all the subjects but one maintained positive Ca balance with normal Ca diet. (Korean J Nutrition 34(1):54-61, 2001)

• 한국전자통신학회논문지
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• 제16권3호
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• pp.533-540
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• 2021

시스템의 성능을 평가하기 위하여 1차원 3-이웃 셀룰라 오토마타(Cellular Automata, 이하 CA) 기반의 의사 난수 생성기가 여러 분야에서 많이 응용되고 있다. 보다 더 효과적인 키 수열 생성을 위해 2차원 CA와 1차원 5-이웃 CA가 응용되었으나, 주어진 특성 다항식에 대응하는 대칭 1차원 5-이웃 CA를 설계하는 것은 매우 어려운 문제이다. 이를 해결하기 위해 특성 다항식의 점화식을 이용한 합성 방법, Krylov 행렬을 이용한 합성 방법과 같이 1차원 5-이웃 CA 합성에 관한 연구들이 진행되었다. 그러나 여전히 비선형 방정식을 풀어야 하는 문제점이 있었다. 이러한 문제점을 해결하기 위해, 최근 90/150 CA의 전이 행렬과 블록행렬을 이용한 1차원 5-이웃 CA 합성 방법이 제안되었다. 본 논문에서는 제안된 알고리즘의 이론적인 과정을 상세히 기술하고 그 알고리즘을 이용하여 높은 차수의 원시 다항식에 대응하는 대칭 1차원 5-이웃 CA를 구한다.

• Molecules and Cells
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• 제20권2호
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• pp.247-255
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• 2005

Three different catalase cDNA clones (CaCat1, CaCat2, and CaCat3) were isolated from hot pepper (Capsicum annuum L.), and their expression patterns were analyzed at the levels of mRNA and enzyme activity. Northern hybridization showed that the three catalase genes were differentially expressed in various organs, and that expression of CaCat1 and CaCat2 was regulated differently by the circadian rhythm. In situ hybridization revealed different spatial distributions of CaCat1 and CaCat2 transcripts in leaf and stem. In response to wounding and paraquat treatment, CaCat1 mRNA increased at 4-12 h in both paraquat-treated and systemic leaves. In contrast, wounding had no significant effect on expression of the catalase genes. The increase of catalase activity in the paraquat-treated and systemic leaves paralleled that of CaCat1 mRNA, but did not match that of CaCat1 mRNA in paraquat-treated stems. Our results suggest that CaCat1 may play a role in responses to environmental stresses.

• Molecules and Cells
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• 제43권6호
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• pp.572-580
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• 2020

Transient receptor potential ankyrin 1 from rattlesnakes (rsTRPA1) and boas (bTRPA1) was previously proposed to underlie thermo-sensitive infrared sensing based on transcript enrichment in infrared-sensing neurons and hyper-thermosensitivity expressed in Xenopus oocytes. It is unknown how these TRPA1s show thermosensitivities that overwhelm other thermoreceptors, and why rsTRPA1 is more thermosensitive than bTRPA1. Here, we show that snake TRPA1s differentially require Ca²⁺ for hyper-thermosensitivity and that predisposition to cytosolic Ca²⁺ potentiation correlates with superior thermosensitivity. Extracellularly applied Ca²⁺ upshifted the temperature coefficients (Q10s) of both TRPA1s, for which rsTRPA1, but not bTRPA1, requires cytosolic Ca²⁺. Intracellular Ca²⁺ chelation and substitutive mutations of the conserved cytosolic Ca²⁺-binding domain lowered rsTRPA1 thermosensitivity comparable to that of bTRPA1. Thapsigargin-evoked Ca²⁺ or calmodulin little affected rsTRPA1 activity or thermosensitivity, implying the importance of precise spatiotemporal action of Ca²⁺. Remarkably, a single rattlesnake-mimicking substitution in the conserved but presumably dormant cytosolic Ca²⁺-binding domain of bTRPA1 substantially enhanced thermosensitivity through cytosolic Ca²⁺ like rsTRPA1, indicating the capability of this single site in the determination of both cytosolic Ca²⁺ dependence and thermosensitivity. Collectively, these data suggest that Ca²⁺ is essential for the hyper-thermosensitivity of these TRPA1s, and cytosolic potentiation by permeating Ca²⁺ may contribute to the natural variation of infrared senses between rattlesnakes and boas.

• 한국전자통신학회논문지
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• 제5권1호
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• pp.10-16
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• 2010

전이 규칙 90과 150만을 사용하는 90/150 CA는 최소다항식과 특성다항식이 같은 CA로 랜덤성이 우수하여 LFSR의 대안으로 사용되어왔다. 90 Uniform CA와 150 uniform CA는 모든 셀에 동일한 전이규칙이 적용되는 CA로 기밀성과 인증을 제공하는 Sarkar의 암호기법에 사용되었다. 본 논문에서는 전이규칙이 90 또는 150인 uniform CA에 대하여 분석하고 특별한 전이규칙을 갖는 n-셀 90/150 CA를 이용하여 2n-셀 uniform CA와 (2n+1)-셀 uniform CA를 합성하고 대응하는 특성다항식을 계산하는 효율적 방법을 제안한다.

• Bulletin of the Korean Chemical Society
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• 제20권3호
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• pp.337-340
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• 1999

Luminescence of bismuth activated CaS, CaS:Bi, prepared in sodium polysulfide is studied. Excitation spectrum of CaS:Bi shows a band at 350 nm due to the recombination process between holes in Na+Ca2+ and electrons in conduction bands, in addition to bands at 260 nm from band gap of CaS, and at 320 nm (1S0→1P1) and at 420 nm (1S0→3P1) from electronic energy transitions of Bi. Emission band at 450 nm is from 3P1→1S0 transition of Bi3+, bands at 500 nm and 580 nm correspond to recombinations of electron donors (Bi3+Ca2+ and VS2-) with acceptors (VCa2+ and Na+Ca2+). Emission band of 3P1→1S0 transition is shifted to longer wavelength from CaS:Bi to BaS:Bi, due to the increase of the Stokes shift by the decrease of the crystal field parameter from CaS:Bi to BaS:Bi.

• Journal of Applied Biological Chemistry
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• 제43권1호
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• pp.5-11
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• 2000

Three cDNA clones encoding rice calmodulin (CaM) isoforms (OsCaM-1, OsCaM-2, and OsCaM-3) were isolated from a rice cDNA library constructed from suspension-cultured rice cells treated with fungal elicitor. The coding regions of OsCaM-1 and O.sCaM-2 were 89% homologous at DNA Ievel, whereas the 5' and 3' untranslated regions were highly divergent. The polypeptides encoded by OsCaM-1 and OsCaM-2 was identical except two conservative substitution at position 8 and 75. The coding region of OsCaM-3 was consist of a typical conserved CaM domain and an additional C-terminal extension. The amino acid sequence of conserved CaM domain of OsCaM-3 shared only 86% identity with that OsCaM-1. The OsCaM-3 cDNA is belongs to a novel group of calmodulin gene due to its C-terminal extension of 38 amino acids, a large number of which are positively charged. The extension also contains a C-terminal CaaX-box prenylation site (CVlL). Genomic Southern analysis revealed at least six copies of CaM or CaM-related genes, suggesting that calmodulin may be represented by a small multigene family in the rice geneme. Expression of OsCaM gene was examined through Northern blot analysis. Transcript level of OsCaM-3 was increased by treatment with a fungal elicitor, whereas the OsCaM-1 and OsCaM-2 genes did not respond to the fungal elicitor. The expression of OsCaM-3 gene was remarkable inhibited in the rice cells treated with cyclosporine A, calcinurin inhibitor.

• 한국전기전자재료학회논문지
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• 제23권4호
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• pp.323-326
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• 2010

$(Zn_{1-x}Ca_x)_2SiO_4$:Mn phosphors doped with Ca were synthesized by solid state reaction method. $(Zn_{1-x}Ca_x)_2SiO_4$:Mn phosphors showed XRD patterns of Willemite structure. Also, $CaSiO_3$ structure and new peak near 610 nm in $(Zn_{1-x}Ca_x)_2SiO_4$:Mn with increasing value of x were observed from XRD and PL. The new peak near 610 nm in $(Zn_{1-x}Ca_x)_2SiO_4$:Mn with doping Ca was attributed to formation of $CaSiO_3$.