Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Prevention of quality deterioration of concentrated blueberry juice by means of pH regulators during thermal treatment

pH 조절제를 이용한 농축 블루베리주스 열처리 시 품질저하억제

  • Lee, In Gyeong (Department of Food Engineering, Dankook University) ;
  • Min, Seo Cheol (Department of Food Science and Technology, Seoul Women's University) ;
  • Kim, Hee Sun (National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Gwi Jung (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Myung Hwan (Department of Food Engineering, Dankook University)
  • 이인경 (단국대학교 식품공학과) ;
  • 민세철 (서울여자대학교 식품공학과) ;
  • 김희선 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 한귀정 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김명환 (단국대학교 식품공학과)
  • Received : 2016.06.17
  • Accepted : 2016.07.21
  • Published : 2016.10.31
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Abstract

This study was conducted to minimize the quality degradation of concentrated blueberry juice after $90^{\circ}C$ treatment for 5 min by means of citric acid (CA) and acidic sodium metaphosphate (ASM) as pH modifiers. The highest anthocyanin content was observed at 1% CA+1% ASM (65.747 mg per 100 g) followed by 1% CA (46.022 mg per 100 g) and control (30.864 mg per 100 g). The DPPH radical scavenging activities of 1% CA+1% ASM, 1% CA and control were 87.338, 75.486 and 67.983% in respectively. The elastase inhibitory activity at 1% CA+1% ASM, 1% CA and in the control was 87.338, 75.486 and 67.983%, and the tyrosinase inhibitory of these samples were 77.891, 67.598 and 26.375%, respectively. Anthocyanin contents, DPPH radical scavenging, elastase inhibitory and tyrosinase inhibitory activities were significantly different (p<0.05) between the 1% CA+1% ASM treatment and control. During the heat treatment, quality degradation of concentrated blueberry juice was reduced by the 1% CA+1% ASM treatment, as expected for new acidulants.

본 연구는 농축 블루베리주스를 제조 시 구연산(CA) 또는 산성메타인산나트륨(ASM)을 산미료로써 이용하여 열처리과정에서 블루베리의 폴리페놀과 안토사이아닌과 같은 기능성 성분농도와 산화방지활성, elastase 억제활성 및 tyrosinase 억제활성 등의 저하를 최소화시키는데 있다. 열처리한 농축 블루베리주스의 색가는 1% CA+1% ASM 처리군이 54.672로 가장 높았고, 1% CA 처리군이 33.034, 대조군이 22.083의 순으로 나타났다. 총 안토사이아닌 함량에서도 1% CA+1% ASM 처리군이 65.747 mg/100 g, 1% CA 처리군, 대조군 순으로 각각 46.022, 30.864 mg/100 g으로 나타났다. 산화방지활성으로써 DPPH radical 소거능의 경우 대조군은 67.983%, 1% CA 처리군은 75.486%, 1% CA+1% ASM 처리군은 87.338%로 나타났다. Elastase 억제활성에서는 대조군, 1% CA, 1% CA+1% ASM 처리군의 결과 값은 각각 26.063, 26.336, 28.285%로 나타났다. Tyrosinase 억제활성에서는 대조군 26.375%, 1% CA 처리군 67.598%, 1% CA+1% ASM 처리군은 77.891% 순으로 높게 나타났다. 모든 분석 치에서 대조군과 1% CA+1% ASM 처리군 간에는 5% 내에서 유의성 차이가 있었다. 본 결과를 통해 ASM처리가 열처리과정에서 기능성성분과 생리활성의 저하를 억제하여주는 역할을 하는 것으로 나타났으며 새로운 산미료로서 활용할 수 있을 것으로 기대한다.

Keywords

References

  1. Kim TC, Bae KS, Kim IK, Chun HJ. Antioxidative activities of solvent extracts from blueberry. J. Orient. Med. Physiol. 19: 179- 183 (2005)
  2. Gao L, Mazza G. Quantitation and distribution of simple and acylated anthocyanins and other phenolics in blueberries. J. Food Sci. 59: 1057-1059 (1994) https://doi.org/10.1111/j.1365-2621.1994.tb08189.x
  3. Su MS, Chien PJ. Antioxidant activity, anthocyanins, and phenolics of rabbiteye blueberry (Vaccinium ashei) fluid products as affected by fermentation. Food Chem. 104: 182-187 (2007) https://doi.org/10.1016/j.foodchem.2006.11.021
  4. Martineau LC, Couture A, Spoor D, Benhaddou-Andoaloussi A, Harris C, Meddah B, Leduc C, Burt A, Vuong T, Le PM, Prentki M, Bennett SA, Amason JT, Haddad PS. Anti-diabetic properties of the Canadian lowbush blueberry Vaccinium angustifolium. Ait. Phytomedicine 13: 612-623 (2006) https://doi.org/10.1016/j.phymed.2006.08.005
  5. Magdalini AP, Andriana D, Zachroula IL, Paul C, Dorothy K, Marigoula M, Fotini NL. Effect of a polyphenol-rich wild blueberry extract on cognitive performance of mice, brain antioxidant markers and acetycholinesterase activity. Behav. Brain. Res. 198: 352-358 (2009) https://doi.org/10.1016/j.bbr.2008.11.013
  6. Zheng W, Wang SY. Oxygen radical absorbinding capacity of phenolics in blueberries, cranberries, chokeberries, and ligoberries. J. Agr. Food Chem. 50: 502-509 (2003)
  7. Gsziano J, Manson JE, Branch LG, Colditz GA, Willett WC, Buring JE. A prospective study of consumption of carotenoids in fruits and vegetables and decreased cardiovascular mortality in the elderly. Ann. Epidemiol. 5: 255-260 (1995) https://doi.org/10.1016/1047-2797(94)00090-G
  8. Prior RL, Wu XL. Anthocyanins: Structure characteristics that result in unique metabolic patterns and biological activities. Free Radic. Res. 40: 1014-1028 (2006) https://doi.org/10.1080/10715760600758522
  9. Routray W, Orsat V. Blueberries and their anthocyanins: Factors affecting biosynthesis and properties. Compr. Rev. Food Sci. Food Saf. 10: 303-320 (2010)
  10. Castaneda-Ovando A, Pacheco-Hernanddez MDL, Paez-Hernandez ME, Rodriguez JA, Galan-Vidal CA. Chemical studies of anthocyanin: A review. Food Chem. 113: 859-871 (2009) https://doi.org/10.1016/j.foodchem.2008.09.001
  11. Martynenko A, Chen Y. Degradation kinetics of total anthocyanins and formation of polymeric color in blueberry hydrothermodynamic (HTD) processing. J. Food Eng. 171: 44-51 (2016) https://doi.org/10.1016/j.jfoodeng.2015.10.008
  12. Sui X, Dong X, Zhou W. Combined effect of pH and high temperature on the stability and antioxidant of two anthocyanins in aqueous solution. Food Chem. 163: 163-170 (2014) https://doi.org/10.1016/j.foodchem.2014.04.075
  13. Martinez M, Whitaker JR. The biochemistry and control of enzymatic browning. Trends Food Sci. Technol. 6: 195-200 (1995) https://doi.org/10.1016/S0924-2244(00)89054-8
  14. KFDA. Korean Food Additives Codex. Seoul, Korea. pp. 929-930 (2014)
  15. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16: 144-158 (1965)
  16. Lee J, Dutst RW, Wrolstad RE. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study. J. AOAC Int. 88: 1269-1278 (2005)
  17. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  18. Marklund S, Gudrun M. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutases. Eur. J. Biochem. 47: 469-474 (1974) https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  19. Gutterridge JM. Reactivity of hydroxyl and hydroxyl like radicals discriminated by release of thiobarbituric acid reactive material from deoxy sugars, nucleosides and benzoate. Biochem. J. 224: 761-767(1984) https://doi.org/10.1042/bj2240761
  20. Muller HE. Detection of hydrogen peroxide produced by microorganism on ABTS peroxidase medium. Zentralbl. Bakteriol. Mikrobiol. Hyg. A. 259: 151-158 (1985)
  21. James AEK, Timothy DWC, Gordon L. Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochem. 35: 9090-9096 (1996) https://doi.org/10.1021/bi953013b
  22. Hearing VJ. Mammalin monophenol monoxygenase (tyrosinase): Purification, properties and reaction catalyzed. Methods Enzymol. 142: 154-165 (1987) https://doi.org/10.1016/S0076-6879(87)42024-7
  23. Torskangerpoll K, Anderson OM. Color stability of anthocyanins in aqueous solutions at various pH values. Food Chem. 89: 427- 440 (2005) https://doi.org/10.1016/j.foodchem.2004.03.002
  24. He J, Giusti MM. High-purity isolation of anthocyanins mixtures from fruits and vegetables-A novel solid-phase extraction method using mixed mode cation-exchange chromatography. J. Chrom. atogr. 1218: 7914-7922 (2011) https://doi.org/10.1016/j.chroma.2011.09.005
  25. Guillotin S, Sanoner P, Renard CMGC. Stabilization of the color of anthocyanin in solutions by mixture with phytocomponents from apple. J. Hortic. Sci. Biotechnol. 84: 96-99 (2009) https://doi.org/10.1080/14620316.2009.11512603
  26. Kim HS, Kang EJ, Kim WS, Kim MH. Study to find the optimal purification processing conditions of anthocyanin from Bokbunja Byproducts. Food Eng. Prog. 18: 25-31 (2014) https://doi.org/10.13050/foodengprog.2014.18.1.25
  27. Pan YZ, Guan Y, Wei ZF, Peng X, Li TT, Qi XL, Zu YG, Fu YJ. Flavonoid C-glycosides from pigeon leaves as color and anthocyanin stabilizing agent in blueberry juice. Ind. Crops. Prod. 58: 142-147 (2014) https://doi.org/10.1016/j.indcrop.2014.04.029
  28. Hager TJ, Howard LR, Prior RL. Processing and storage effects on monomeric anthocyanins, percent polymeric color, and antioxidant capacity of processed blackberry products. J. Agric. Food. Chem. 56: 689-695 (2008) https://doi.org/10.1021/jf071994g
  29. Tsuji N, Moriwaki S, Suzuki Y, Takemura Y, Imokawa G. The role of elastases secreted by fibroblasts in wrinkle formation; implication through selective inhibition of elastase activity. Photochem. and Photobiol. 74: 283-287 (2001) https://doi.org/10.1562/0031-8655(2001)074<0283:TROESB>2.0.CO;2
  30. Kim SH, Ahn JH, Jeong JY, Kim SB, Jo YH, Hwang BY, Lee MK. Tyrosinase inhibitory phenolic constituents of Smilax China leaves. Kor. J. Pharmacogn. 44: 220-223 (2013)