• Title/Summary/Keyword: C : N : P

Search Result 7,869, Processing Time 0.035 seconds

Analysis of antigenic domain of GST fused major surface protein (p30) fragments of Toxoplasma gondii (융합단백질로 발현된 톡소포자충의 주요막단백질(p30) 절편의 항원성)

  • 남호우;임경심
    • Parasites, Hosts and Diseases
    • /
    • v.34 no.2
    • /
    • pp.135-142
    • /
    • 1996
  • Antigenic domain of jai or surface protein (p30) of Toxoplosmc Sondii was analyzed after polymerase chain reaction (PCR) of its gene fragments. Hydrophilic or hydrophobic moiety of amino acid sequences were expressed as glutathione S-transferase (G57) fusion proteins. Fragments of p30 gene were as follows: 737, total p30 open reading frame (ORF) ; S28, total ORF excluding N-terminal signal sequence and C-terminal hydrophobic sequence; Al9, N-terminal 2/3 parts of A28; A19, N-terminal 2/3 of S28; P9, C-terminal 2/3 part of S28; Z9. middle 1/3 of S28; and 29, C-terminal 1/3 of S28. respectively. Primer of each fragment was synthesized to include clamp sequence of EcoR I restriction site. PCR amplified DNA was inserted info GST (26 kDa) expression vector, PGEX-47-1 to transform into Escheri,hia coei (.JM105 strain). G57 fusion proteins were expressed with IPTG induction as 63. 54, 45, 45, 35, 36. and 35 kDa proteins measured by SDS-PAGE. Each fusion protein was confirmed with G57 detection kit. Western blot analysis with the serum of a toxoplasmosis patient revealed antigenicity in proteins expressed by T37. S28, and Al9 but not those by Pl8. X9, Y10, and Z9. Antigenicity of p30 seems to be located either in N-terminal 115 part in the presence of middle 1/3 part or in the oligopeptides between margins of the first and second 1/3 parts.

  • PDF

Poly(n-butyl acrylate-co-methyl methacrylate) and Poly(n-butyl acrylate-co-styrene)/Silicate Nanocomposites Prepared by Emulsion Polymerization

  • Park, Yeong-Suk;Chung, In-Jae
    • Macromolecular Research
    • /
    • v.11 no.6
    • /
    • pp.425-430
    • /
    • 2003
  • Two types of poly(n-butyl acrylate) copolymer/silicate nanocomposites have been produced: poly(n-butyl acrylate-co-methyl methacrylate) [P(BA-co-MMA)]/silicate nanocomposites and poly(n-butyl acrylate-co-styrene) [P(BA-co-ST)]/silicate nanocomposites. The P(BA-co-MMA)/silicate nanocomposites shows the exfoliated structures but a P(BA-co-ST)/silicate nanocomposites have intercalated structures, because the BA/MMA comonomer has a higher polarity (e-value in Q-e scheme) than the BA/ST comonomer. The BA/MMA comonomer expanded the interlayer space of the silicate wider than did the BA/ST comonomer. The thermal degradation onset point of the P(BA-co-MMA)/silicate nanocomposites was 43$^{\circ}C$ higher than that of pure P(BA-co-MMA). P(BA-co-MMA)T5%, P(BA-co-MMA)T10%, and P(BA-co-MMA)T20% exhibit 134,302, and 195% increases, respectively, in their storage moduli at -20$^{\circ}C$ relative to the pure copolymer.

The Crystal and Molecular Structure of P-Aminobenzaldehyde Cyclohexylthiosemicarbazone (P-Aminobenzaldehyde Cyclohexylthiosemicarbazone의 결정 및 분자구조)

  • Chung Hoe Koo;Chong Hee Kim;Young Ja Park
    • Journal of the Korean Chemical Society
    • /
    • v.25 no.6
    • /
    • pp.343-350
    • /
    • 1981
  • The crystal and molecular structure of P-aminobenzaldehyde cyclohexylthiosemicarbazone, C14H20N4S, has been determined from 2712 integrated intensities measured on a computer controlled four circle diffractometer with monochromated $CuK_{\alpha}$, X-ray radiation. The crystals are monoclinic, space group C2/c with eight molecules in a unit cell of dimensions, a = 12.488(2), b = 12.276(4), c = 19.997(6)${\AA}$ and ${\beta}=103.55(3)^{\circ}$. The structure was solved by Patterson and Fourier method and refined by a full-matrix least squares method to a final R value of 0.058 for all reflections. The C(8)-S bond is trans to N(2)-N(3) and C(8)-N(1) is cis to N(2)-N(3) bond. The cyclohexane ring has chair conformation and makes an angle of $40.7^{\circ}$ with the benzene ring. The molecules are linked by N(2)H…S hydrogen bonds into dimer-like units which are held together by $N-H{\ldots}N$ hydrogen bonds. Sulfur accepts second rather weak hydrogen bond from N(4). An intramolecular hydrogen bond exists between N(1) and N(3) atoms.

  • PDF

LEAST SQUARES SOLUTIONS OF THE MATRIX EQUATION AXB = D OVER GENERALIZED REFLEXIVE X

  • Yuan, Yongxin
    • Journal of applied mathematics & informatics
    • /
    • v.26 no.3_4
    • /
    • pp.471-479
    • /
    • 2008
  • Let $R\;{\in}\;C^{m{\times}m}$ and $S\;{\in}\;C^{n{\times}n}$ be nontrivial unitary involutions, i.e., $R^*\;=\;R\;=\;R^{-1}\;{\neq}\;I_m$ and $S^*\;=\;S\;=\;S^{-1}\;{\neq}\;I_m$. We say that $G\;{\in}\;C^{m{\times}n}$ is a generalized reflexive matrix if RGS = G. The set of all m ${\times}$ n generalized reflexive matrices is denoted by $GRC^{m{\times}n}$. In this paper, an efficient method for the least squares solution $X\;{\in}\;GRC^{m{\times}n}$ of the matrix equation AXB = D with arbitrary coefficient matrices $A\;{\in}\;C^{p{\times}m}$, $B\;{\in}\;C^{n{\times}q}$and the right-hand side $D\;{\in}\;C^{p{\times}q}$ is developed based on the canonical correlation decomposition(CCD) and, an explicit formula for the general solution is presented.

  • PDF

A Study on the Oxide Semiconductor $ITO_{(n)}/Si_{(p)}$ Solar Cell(I) (산화물 반도체 $ITO_{(n)}/Si_{(p)}$ 태양전지에 관한 연구(I))

  • Kim, Y.W.;Lee, D.C.
    • Proceedings of the KIEE Conference
    • /
    • 2002.07b
    • /
    • pp.1325-1327
    • /
    • 2002
  • $ITO_{(n)}/Si_{(p)}$ solar cell was fabricated by vaccum deposition method under the resistance heating with substrate temperature kept about 200[$^{\circ}C$] and than their properties are investigated. The cell charateristics can be improved by annealing but are deteriorated at temperature above 650[$^{\circ}C$] for longer than 15[min].

  • PDF

MONOTONICITY PROPERTIES OF THE GENERALIZED STRUVE FUNCTIONS

  • Ali, Rosihan M.;Mondal, Saiful R.;Nisar, Kottakkaran S.
    • Journal of the Korean Mathematical Society
    • /
    • v.54 no.2
    • /
    • pp.575-598
    • /
    • 2017
  • This paper introduces and studies a generalization of the classical Struve function of order p given by $$_aS_{p,c}(x):=\sum\limits_{k=0}^{\infty}\frac{(-c)^k}{{\Gamma}(ak+p+\frac{3}{2}){\Gamma}(k+\frac{3}{2})}(\frac{x}{2})^{2k+p+1}$$. Representation formulae are derived for $_aS_{p,c}$. Further the function $_aS_{p,c}$ is shown to be a solution of an (a + 1)-order differential equation. Monotonicity and log-convexity properties for the generalized Struve function $_aS_{p,c}$ are investigated, particulary for the case c = -1. As a consequence, $Tur{\acute{a}}n$-type inequalities are established. For a = 2 and c = -1, dominant and subordinant functions are obtained for the Struve function $_2S_{p,-1}$.

On characterizations of real hypersurfaces of type B in a complex hyperbolic space

  • Ahn, Seong-Soo;Suh, Young-Jin
    • Journal of the Korean Mathematical Society
    • /
    • v.32 no.3
    • /
    • pp.471-482
    • /
    • 1995
  • A complex n-dimensional Kaehlerian manifold of constant holomorphic sectional curvature c is called a comples space form, which is denoted by $M_n(c)$. A complete and simply connected complex space form consists of a complex projective space $P_nC$, a complex Euclidean space $C^n$ or a complex hyperbolic space $H_nC$, according as c > 0, c = 0 or c < 0. The induced almost contact metric structure of a real hypersurface M of $M_n(c)$ is denoted by $(\phi, \zeta, \eta, g)$.

  • PDF

Impact of Slash and Burning on Microbial Biomass in Semi-Evergreen Tropical Deciduous Forest of Manipur, North-East India

  • Yadava, P.S.;Devi, A.Sarjubala
    • The Korean Journal of Ecology
    • /
    • v.27 no.4
    • /
    • pp.225-230
    • /
    • 2004
  • The impact of slash and burning on microbial biomass C, N and P in soils of semi-evergreen tropical deciduous forest were studied from February 1999 to January 2000. The experimental sites were located near Moreh town in the Chandel district of Manipur state (India) along the Indo-Myanmar border between 23° 49' N-24°28'N latitude and 93°45'E-94°16'E longitude. Microbial biomass C ranged from 319.50 ㎍ g/sup -1/ 905.50㎍ g/sup -1/ in the slash and burnt site and from 209.50 ㎍ g/sup -1/ to 708.80 ㎍ g/sup -1/ soil in the forest site. Microbial N ranged from 19.30 ㎍ g/sup -1/ to 99.45 ㎍ g/sup -1/ in the slash and burnt site and from 16.08㎍ g/sup -1/ to 88.90 ㎍ g/sup -1/ in the forest site. Microbial P varied from 10.90 ㎍ g/sup -1/ to 32.21 ㎍ g/sup -1/ in the slash and burnt site and from 2.50 ㎍ g/sup -1/ to 17.60 ㎍ g/sup -1/ in the forest site in different months throughout the year. Microbial biomass C, N and P were recorded to be higher in the slash and burnt site compared to the forest site The conversion of forest into slash and burnt site for agriculture - the traditional shilling cultivation practiced by tribal people in the north- eastern India leads to addition of large amount of organic matter in the soil thereby exhibiting higher values of microbial biomass C, N and P in the recent slash and burnt site than that of the forest site. Relationship between the soil moisture, soil organic C and microbial biomass C, N and P were found to be correlated significantly in both the sites.

Modulation of Calcium Current by Cyclic GMP in the Single Ventricular Myocytes of the Rabbit (토끼 단일 심실근 세포에서 cyclic GMP의 $Ca^{2+}$ 전류 조절기전에 관한 연구)

  • An, Jae-Ho;Seo, Gyeong-Pil;Eom, Yung-Ui
    • Journal of Chest Surgery
    • /
    • v.25 no.4
    • /
    • pp.364-382
    • /
    • 1992
  • In order to investigate the effect of intracellular cyclic GMP on the calcium channel, whole cell patch clamp technique with internal perfusion method was used in the single ventricular myocytes of the rabbit. Cyclic GMP, cGMP analogues, cAMP, isopernaline and forskolin were perfused into cells and their effects on the calcium current were analysed by applying depolarizing step pulse of 10 mV in amplitude for 200 msec from holding potential of -40 mV. Calcium currents usually activated from -30 mV and then reached a peak at +10 mV. Amplitude of the calcium current was standardized with membrane capacitance, 50 pF. Peak amplitude at +10 mV in control was -0.15 nA/50pF. When 100 mM cAMP was applied from the pipette, peak amplitude of calcium current increased to -0.32 nA and addition of 1 mM isoprenaline further increased its amplitude. In the presence of cGMP it alone also produced an increase of the calcium current to -0.52 nA/50pF and addition of isoprenaline or forskolin increased its magnitude to -[0.55~0.95] nA/50pF. Simultaneous application of cGMP and cAMP increased the calcium current to -0.67 nA/50pF. Among the cGMP analogues, 8-Br-cGMP was the most potent stimulant for the calcium current activation. From the above results it could be concluded tlat cGMP increases the calcium current not through cAMP dependent protein kinase nor cAMP dependent phosphodiesterase pathway, but through independent phosphorylation pathway, possibly cGMP dependent protein kinase pathway.

  • PDF

Fixed points of a certain class of mappings in uniformly convex banach spaces

  • Thakur, Balwant-Singh;Dep
    • Bulletin of the Korean Mathematical Society
    • /
    • v.34 no.3
    • /
    • pp.385-394
    • /
    • 1997
  • In this paper, we prove in p-uniforlmy convex space a fixed point theorem for a class of mappings T satsfying: for each x, y in the domain and for n = 1, 2, 3, $\cdots$, $$ \left\$\mid$ T^n x - T^n y \right\$\mid$ \leq a \cdot \left\$\mid$ x - y \right\$\mid$ + b(\left\$\mid$ x - T^n x \right\$\mid$ + \left\$\mid$ y - T^n y \right\$\mid$) + c(\left\$\mid$ c - T^n y \right\$\mid$ + \left\$\mid$ y - T^n x \right\$\mid$, $$ where a, b, c are nonnegative constants satisfying certain conditions. Further we establish some fixed point theorems for these mappings in a Hilbert space, in $L^p$ spaces, in Hardy spaces $H^p$ and in Sobolev spaces $H^{p,k}$ for 1 < p < $\infty$ and k $\leq$ 0. As a consequence of our main result, we also the results of Goebel and Kirk [7], Lim [8], Lifshitz [12], Xu [20] and others.

  • PDF