• Genes and Genomics
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• v.40 no.12
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• pp.1259-1267
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• 2018

Litchi (Litchi chinensis Sonn.) is an important subtropical fruit crop with high commercial value due to its high nutritional values and favorable tastes. However, irregular bearing attributed to unstable flowering is a major ongoing problem for litchi producers. Previous studies indicate that low-temperature is a key factor in litchi floral induction. In order to reveal the genetic and molecular mechanisms underlying the reproductive process in litchi, we had analyzed the transcriptome of buds before and after low-temperature induction using RNA-seq technology. A key flower bud differentiation associated gene, a homologue of FLORICAULA/LEAFY, was identified and named LcLFY (GenBank Accession No. KF008435). The cDNA sequence of LcLFY encodes a putative protein of 388 amino acids. To gain insight into the role of LcLFY, the temporal expression level of this gene was measured by real-time RT-PCR. LcLFY was highly expressed in flower buds and its expression correlated with the floral developmental stage. Heterologous expression of LcLFY in transgenic tobacco plants induced precocious flowering. Meantime, we investigated the sub-cellular localization of LcLFY. The LcLFY-Green fluorescent protein (GFP) fusion protein was found in the nucleus. The results suggest that LcLFY plays a pivotal role as a transcription factor in controlling the transition to flowering and in the development of floral organs in litchi.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.645-648
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• 2010

A new spray carnation cultivar 'Lyra' was released by the National Institute of Horticultural and Herbal Science in 2003. A cross was made between spray cultivar 'Ballantyne' and red spray cultivar 'Chateau' in 1999. After investigation of the characteristics during four years (from 2000 to 2003), the cultivar 'Lyra' was finally selected in 2003. 'Lyra' is a spray cultivar with bright pink color for a cut flower. The main characteristics are early flowering, multi branching, fragrance and long flower stalk. Also, it has medium resistance to Fusarium wilt and 7 days vase life. To avoid calyx splitting caused by high temperature, it is recommended that this cultivar be grown over $8^{\circ}C$ at night and under $25^{\circ}C$ at day time during a flower bud differentiation period and applied with additional nitrate fertilization ($30.0kg{\cdot}10^{-1}$).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.2
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• pp.195-200
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• 1985

This experiment was carried out to know the processes of protoplast isolation, culture and plant regeneration in aims of introducing foreign genes into plant cells through plant gene vector, and cellular selection for plant improvement. The main results indicated that 2% cellulase plus 0.5% macerozyme is proper for isolation of protoplasts from leaf mesophyll cells of N. plumbaginifolia, plating efficiency was higher in 1.4-2.0 x 10$^4$ cells/ml, complete cell wall was regenerated after 2 days culture, cell division and cell mass were observed after 4 days and 2 weeks, respectively, colony was developed after 3 weeks culture, addition of 1-2mg/l BA promoted shoot differentiation while root differentiation did not required hormone and seeds were harvested from more than 100 cell lines for further investigation and study.

• Journal of Sericultural and Entomological Science
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• v.20 no.2
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• pp.10-14
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• 1978

There are relatively many reports have been issued on he active movement of cuticle of larva. which tend to protect their body, however, only a few reports have been disclosed on the cuticle of pupal body except the small portion of rectangle which shown tortoise-shell shape. In this connection, many portion of the external structure of pupal cuticle has been studied and the following. results were found: 1) No. spot of rectangle which is sculptured in the surface of cuticle that born by branching. out of the development of cell in imaginal bud of antenna and head were found. However, in the compound eye of net shaped sculpture was found in the (equation omitted) shaped parts which holding. the diameter of about 8u and the surrounding area has the small bump and the one is dark brown coloured comparing with shape. 2) The sculpture shape of thorax is a little different than in the head. However, (equation omitted) portion is varies from the segment to segment. In general, it is not very clear than the compound eye in the head, the dark brown bump shape is slowly fade a from the prothorax, mesothorax to metathorax. 3) The surface of intersegment membrane is colourless or slightly yellow, and the entire surface has stripped marking with thine lines. 4) In the abdominal segment, there are many and small sculptures in net shape around the (equation omitted) shape portions. 5) The size of sculpture in (equation omitted) portion of abdomen is smaller than one in thorax, and in the same segment, the dorsal is smaller than abdomen and the rear portion of the segment is larger than the front of segment. 6) After the 7th abdominal segment, no intersegmental membrane is found and the cuticle of the external structure is the same as external structure of the segment. 7) The seta is not found in head, compound eye, antenna and wing which portions were subdivided by development of imagined bud of the cell, no seta is found in cuticle of the segment in the general cell of the larva stage and also in the dorsal and intersegmental membrane.

• Journal of Bio-Environment Control
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• v.29 no.4
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• pp.421-427
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• 2020

In this study, we investigated the effect of nutrient supply periods on the fruit growth and quality of 'Duke' blueberry. The nutrient solution was contained with NO₃-N 4.6, NH₄-N 3.4, PO₄-P 3.3, K 3.0, Ca 4.6, and Mg 2.2 mmol·L^-1, and EC in the nutrient solution was 1.5 ds.m^-1. In 2017 and 2018, an individual blueberry bush was supplied with 8 L of the nutrient solution per week. In 2018, the drainage water quality of growing medium and fruit quality was investigated. The nutrient supply was started from April 01, and stopped at 15 days intervals as follows, 15 days before final harvest, at the final harvest date (June 30), 15 days after final harvest (DAFH), 30 DAFH, 45 DAFH, and 60 DAFH. The content of inorganic components in the growing medium was not significantly different by the stop time of nutrient supply, but the content of phosphorus (P₂O₅) tended to increase with the delay of stop time of nutrient supply. There were no significant differences in the fruit quality characteristics in terms of size, sugar content, and acidity among the different stop time of nutrient supply. The blueberry yield was tended to decrease with the delay of periods of nutrient supply, while the lowest yield of 1.8 kg was recorded when nutrient supply stopped at 45 and 60 DAFH.

• Horticultural Science & Technology
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• v.33 no.3
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• pp.420-428
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• 2015

The majority of cultivated varieties of grape have perfect flowers that are clustered in an individual inflorescence. Grape flower has a single pistil, five stamens, a protective flower cap (calyptra), and a calyx. After fertilization, an individual flower develops into a single berry. Although there are a number of reported studies focusing on berry formation, berry enlargement, and sugar accumulation in grape, the morphological studies of flower, including gametophyte morphogenesis and structural change in floral organs, have not yet been studied in detail. In this study, we investigated the flower structure and development characteristics of grape using microscopy and defined the floral development stages 9 to 13 based on microspore or male gametophyte development stage from tetrad to mature pollen. We used seeded diploid table grapes 'Campbell Early' (Vitis labruscana) and 'Tamnara' (V. spp.) as plant materials. At floral development stage 9, pollen mother cells develop to tetrads. During floral development stages 10 to 11, unicellular microspore develop to mid bicellular pollen. At the end of floral stage 12, male gametophyte develops to mature tricelluar pollen. In floral stage 13, the flower cap falls off and flower bud opens. During floral development stages 9 to 12, there were no major changes in calyx length, whereas the length of the flower cap continuously increased. The flower cap-to-calyx length ratio was 2.0, 3.0, 4.5, and 6.5 at floral stages 9, 10, 11, and 12, respectively. The flower cap-to-calyx length ratio was consistent in the two grape cultivars, suggesting that the ratio is a morphological character representing floral development stage. This study provides a reference for determining floral development stage of the two grape cultivars. It will be useful for the determination of optimum time for microspore culture needed to generate doubled haploid lines and appropriate gibberellic acid treatment needed to induce parthenocarpic fruit development in 'Tamnara' grape.

• Korean Journal of Agricultural and Forest Meteorology
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• v.18 no.3
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• pp.151-161
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• 2016

This study was conducted to find out influence of dormancy level and carbon concentration on freezing hardiness in bourse shoot of 'Fuji' apple tree. Bourse shoot of 'Fuji' adult apple tree grafted on M.26 and M.9 rootstocks were used as experimental materials. Dormancy levels of bourse shoot were categorized according to the periods as follows the internal dormancy (late January), the early days after internal dormancy breaking (early February), the late days after internal dormancy breaking (late February), the bud break (late March), and the full bloom (late April). Chilling temperatures with bourse shoot were ranged from 0 to $-40^{\circ}C$. Also, the freezing hardiness according to carbon concentrations were investigated on 'Fuji'/M.9 apple tree that defoliated severely by Marssonina blotch (defoliation) and that of below the average 20 cm in shoot length through heavy crop load (weakness). Results showed that freezing hardiness of bourse shoot may become weaker after internal dormancy breaking. There was no differences in the carbon concentration of bourse shoot of 'Fuji' apple tree grafted on M.9 and M.26, so may be resulted in no difference in freezing hardiness both of bourse shoot grafted on M.9 and M.26 rootstock. Carbon concentration in bourse shoots with weakness and C/N ratio in bourse shoots with defoliation were lower than that of healthy. It may be shown that the freezing hardiness of defoliation and weakness were weaker than that of healthy.

• Horticultural Science & Technology
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• v.17 no.3
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• pp.329-332
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• 1999

The objective of this study was to determine the influence of grafting timing, rootstock cut timing and leaf removal with promalin ($GA_{4+7}+BA$) treatments on the maiden tree growth, lateral development and flower bud initiation. In mid-March 1997, two-year-old M.9-T337 rootstocks selected with trunk diameter over 1 cm were planted in the field. Chip budding with 'Fuji' scion on M.9-T337 rootstock budded in mid-April was earlier in sprouting than chip budding in mid-June. Late cutting chip budding (LCCB) with 'Fuji' scion on M.9-T337 rootstock was lower in the failed budding percentage with 14% than that of early cutting chip budding (ECCB). Especially, ECCB in April was not suitable for scion growth such as uniformity with high percentage of failed tree. Grafting timing in mid-June and rootstock cutting timing of LCCB induced more branches and flower buds than other treatments. Removal of 8 to 10 uppermost immature leaves on central leader stem and application of Promalin 250 mg/L after every 30 cm of terminal growth produced a 189 cm tall tree with 9 flower buds and 14.2 short lateral shoot from 30 to 35 cm long in length in 1998. Promalin increased branching on second-season growth and, when combined with leaf removal, resulted in uniform distribution of branches along the central leader stem.

• International Journal of Oral Biology
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• v.35 no.4
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• pp.169-175
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• 2010

Cholinesterase (ChE) is one of the most ubiquitous enzymes and in addition to its well characterized catalytic function, the morphogenetic involvement of ChE has also been demonstrated in neuronal tissues and in non-neuronal tissues such as bone and cartilage. We have previously reported that during mouse tooth development, acetylcholinesterase (AChE) activity is dynamically localized in the dental epithelium and its derivatives whereas butyrylcholinesterase (BuChE) activity is localized in the dental follicles. To test the functional conservation of ChE in tooth morphogenesis among different species, we performed cholinesterase histochemistry following the use of specific inhibitors of developing molar and incisors in the hamster from embryonic day 11 (E11) to postnatal day 1 (P1). In the developing molar in hamster, the localization of ChE activity was found to be very similar to that of the mouse. At the bud stage, no ChE activity was found in the tooth buds, but was first detectable in the dental epithelium and dental follicles at the cap and bell stages. AChE activity was found to be principally localized in the dental epithelium whereas BuChE activity was observed in the dental follicle. In contrast to the ChE activity in the molars, BuChE activity was specifically observed in the secretory ameloblasts of the incisors, whilst no AChE activity was found in the dental epithelium of incisors. The subtype and localization of ChE activity in the dental epithelium of the incisor thus differed from those of the molar in hamster. In addition, these patterns also differed from the ChE activity in the mouse incisor. These results strongly suggest that ChE may play roles in the differentiation of the dental epithelium and dental follicle in hamster, and that morphogenetic subtypes of ChE may be variable among species and tooth types.

• Journal of Korean Society of Forest Science
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• v.79 no.2
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• pp.109-114
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• 1990

The axillary buds of 15-year-old Tilia amurensis were cultured on Saito and Ide (IS), Murashige and Skoog (MS) media and woody plant medium (WPM) to establish an effective micropropagation method. Five levels of 6-benzylaminopurine (BAP) were tested. On IS medium and WPM addition of 1.0/l BAP enhanced shoot development and shoot elongation, whereas addition of 0.5/l BAP was effective on MS medium. A better results were obtained from WPM with 1.0/l BAP and MS with 0.1/l BAP. Developed shoots were subcultured on each basal media but with 0.2/l BAP, Multiple shoots were almost doubled in a month. Root formation could be enhanced at higher concentration of indole-3-butyric acid (IBA). Better rooting rate (83.3%) was achieved on a half-strength MS medium with 3.0 /l IBA. Regenerated plantlets were successfully transferred to soil. To investigate the clonal variation in shoot development and shoot elongation by axillary bud culturing, seven plus tree clones were tested, Clonal variation in tissue culturability among plus trees was recognized by the Duncan's multiple range test at the 5% level. Kang Won No. 12 showed the best response on WPM with 1.0/l BAP.