• Korean Journal of Microbiology
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• v.53 no.4
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• pp.235-241
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• 2017

The purpose of this study was to investigate the enhanced physiological activities of two Pichia strains, yeasts isolated from grapes pericarp. Based on phylogenetic analysis using 18S rRNA sequencing, two isolates were identified as Pichia manshurica GU-3 and Pichia terricola GU-4, respectively. The scanning electron microscopic analysis showed that the two isolates grown on YPD medium were of typical elliptical shape with buds and bud scars on cell surface. Physiological activities of the single and mixed Pichia cultures were monitored and compared. In mixed cultures after 72 h of incubation, the maximum activities of tyrosinase inhibition, ACE inhibition, and antioxidant were 81.7%, 45.9%, and 42.7%, respectively. Superoxide dismutase-like activity was approximately 30% in the mixed cultures. These studies demonstrate that Pichia species cultured in the form of mixture can enhance the physiological activities and has potential for the development of new bioactive products.

• Korean Journal of Medicinal Crop Science
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• v.18 no.2
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• pp.93-97
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• 2010

Cultural practices of Asparagus cochinchinensis in highland area were performed for a potential medicinal crop. These studies were examined to propagation methods, planting densities, nitrogen treatments, and cropping years. The results are summarized as follows. The adequate number of buds per tuberous root was 4 for vegetative propagation because the number of tuberous root harvested was 16.8 and the yield was also the highest, exhibiting 1,060 kg/10a. The suitable planting time for vegetative propagation was later than early April. If the earlier tuberous roots were planted, the less they emerged. The highest emergence rate was obtained from the planting density of $30{\times}20cm$ as 97.2% while the yield was highest in the $30{\times}15cm$ density, exhibiting 1,883 kg/10a with emergence rate at 94.9. It seemed that the higher planting density promoted plant height growth and yield in Asparagus cochinchinensis. The highest fresh weight was recorded at 6 kg/10a of nitrogen fertilizer into the sandy loam soil compared to the level of 0, 3, 9 kg/10a. The yield was increased with cropping years. However, the proper harvesting time was the second year of cultivation because the rate of weight increase was maximized in the 2-year-old tuberous root. The yield in the third year was decreased as compared to that of the second year.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.194-196
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• 2009

A new standard carnation Dianthus caryophyllus 'Pink Beryl' was bred from a cross of red standard cultivar 'Francesco' and purple standard cultivar 'Saturn' in 1996 at the National Institute of Horticultural & Herbal Science. After investigation of the characteristics for 4 years from 1997 to 2000 and registered to the official breeding variety of Rural Development Administration in 2000. 'Pink Beryl' is pink standard cultivar with round edge of petal for cut flower. It has medium resistance to Fusarium wilt. To avoid calyx splitting caused by high temperature, this cultivar is recommended to grow over $8^{\circ}C$ at night and under $25^{\circ}C$ at days during flower bud differentiation period.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.39-44
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• 2010

An efficient method for the rapid propagation of Smilax china from axillary buds was established. Plants with thick leafage were selected from Korea native S. china population. Axillary buds of S. china collected from selected plant and were cultured in various culture media (2MS, MS, 1/2MS, WPM, B5 and SH medium). Shoot was induced from axillary bud on MS basal medium after 4 weeks of culture. 1/2MS medium showed a higher growth rate than those of the others, while the lowest shoot growth was obtained in 2MS medium. Among the sucrose concentrations, 5% sucrose was the optimum level for shoots growth from axillay buds. Among cytokinins, $0.5mgL^{-1}$ 6-benzylaminopurine (BAP) treatment showed the best performance on shoot multiplication, yielding average shoot multiplication forming about 2.4. Rooting was induced directly near the base of the shoot on 1/2MS medium containing with three-auxins ${\alpha}-napthalene$ acetic acid (NAA), indole acetic acid (IAA) and ${\beta}-indolebutyric$ acid (IBA) (0.5 and $1.0mgL^{-1}$). The $1.0mgL^{-1}$ IBA treatments induced earliest rooting with maximum of root number and root growth. These rooted plantlets were successfully transferred to pots for 4 weeks hardening process, and were transferred to soil with above 90% survival rate.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.530-533
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• 2009

This paper proposes a method of recognizing that a person doesn't put on a safety cap using image processing method in DSP(Digital Signal Processor). It processes inputted images by image input devices that equipped in a industrial settings. If the method recognizes a person that doesn't put on a safety cap, a system transfers relevant recognition result to a supervisor and takes proper measures. If an accident happens and someone doesn't put on a safety cap, additional casualities could be. Proposed method can nip additional casualties in the bud. To recognize that a person don't put on a safety cap, images are processed by object abstraction, removal of noise, decision of a thing or a person, abstraction of a head part in a image, recognizing whether a man puts on a safety cap using HSV color space or not, and so on. Image input and image process are processed by DSP. And C language-based codes are optimized by an eignefunction(Intrinsics) for speed improvement of algorithms.

• Genes and Genomics
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• v.40 no.12
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• pp.1259-1267
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• 2018

Litchi (Litchi chinensis Sonn.) is an important subtropical fruit crop with high commercial value due to its high nutritional values and favorable tastes. However, irregular bearing attributed to unstable flowering is a major ongoing problem for litchi producers. Previous studies indicate that low-temperature is a key factor in litchi floral induction. In order to reveal the genetic and molecular mechanisms underlying the reproductive process in litchi, we had analyzed the transcriptome of buds before and after low-temperature induction using RNA-seq technology. A key flower bud differentiation associated gene, a homologue of FLORICAULA/LEAFY, was identified and named LcLFY (GenBank Accession No. KF008435). The cDNA sequence of LcLFY encodes a putative protein of 388 amino acids. To gain insight into the role of LcLFY, the temporal expression level of this gene was measured by real-time RT-PCR. LcLFY was highly expressed in flower buds and its expression correlated with the floral developmental stage. Heterologous expression of LcLFY in transgenic tobacco plants induced precocious flowering. Meantime, we investigated the sub-cellular localization of LcLFY. The LcLFY-Green fluorescent protein (GFP) fusion protein was found in the nucleus. The results suggest that LcLFY plays a pivotal role as a transcription factor in controlling the transition to flowering and in the development of floral organs in litchi.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.645-648
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• 2010

A new spray carnation cultivar 'Lyra' was released by the National Institute of Horticultural and Herbal Science in 2003. A cross was made between spray cultivar 'Ballantyne' and red spray cultivar 'Chateau' in 1999. After investigation of the characteristics during four years (from 2000 to 2003), the cultivar 'Lyra' was finally selected in 2003. 'Lyra' is a spray cultivar with bright pink color for a cut flower. The main characteristics are early flowering, multi branching, fragrance and long flower stalk. Also, it has medium resistance to Fusarium wilt and 7 days vase life. To avoid calyx splitting caused by high temperature, it is recommended that this cultivar be grown over $8^{\circ}C$ at night and under $25^{\circ}C$ at day time during a flower bud differentiation period and applied with additional nitrate fertilization ($30.0kg{\cdot}10^{-1}$).

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.815-826
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• 2018

The intense drought affecting olive production in Northern Chile underscores the need to research non-traditional irrigation strategies to obtain the best crop performance. Accordingly, this study aimed to obtain preliminary data to guide future research on this topic. Different water replenishment levels on crop evapotranspiration ($ET_c$ ; 13.5, 27.0, 40.5, and 54%) were established in a young orchard, cv. Arbequina, from the end of fruit drop (EFD) to full bloom in the next season. We evaluated the influence of plant water status (${\Psi}_{stem}$ ) and crop load, considered as function of fruit number divided by trunk cross-sectional area, on reproductive and productive variables using multiple linear regressions. Our results show that crop load and ${\Psi}_{stem}$ measured from EFD to harvest affected yield components. Nevertheless, ${\Psi}_{stem}$ had the strongest influence on fruit size, pulp development, oil accumulation, and yield. Oil content and yield were reduced by 54% and 50% for each MPa, respectively, from ${\Psi}_{stem\;EFD-H}$ -1.8 MPa, an effect that intensified as crop load increased. During the period of flower development (September-November), the number of flowers per inflorescence and percentage of perfect flowers were reduced when ${\Psi}_{stem}$ was less than -2.0 MPa. These preliminary results showed that bud differentiation, inflorescence and flower formation are highly sensitive to water deficit.

• Korean Journal of Veterinary Research
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• v.58 no.4
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• pp.171-175
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• 2018

The aim of this study was to investigate morphological development of filiform papillae (FP) in Korean native goats by using scanning electron microscopy. Tongues were removed from goat fetuses (days 60, 90, and 120), neonates, and juveniles (days 30, 60, 90, 120, 150, and 180 after birth). During the prenatal period, primordia of FP appeared at fetal day 60 and were observed to be developed at day 90. At fetal day 120, the FP were observed like flower leaves of a double flower bud. In neonates, FP were shaped like an obliquely sectioned cylinder with secondary papillae irregularly arranged in a saw blade-like manner. In 60-day-old juvenile goats, the FP were densely distributed at the inner base of 1/3-1/2 degrees. In 90-, 120-, and 150-day-old goats, FP were compacted at the inner base of 1/2-2/3, 3/4, and 4/5 degrees, respectively. In 180-day-old goats, FP were found to be completely compacted on the inner surface with complete morphogenesis. Microridges, microplicae, and micropits were well-developed on the epithelial surface of lingual papillae from embryonic day 120 to juvenile day 180. These results indicate that FP of goats have different shapes and sizes during development both before and after birth.

• Korean Journal of Environmental Agriculture
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• v.40 no.2
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• pp.73-82
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• 2021

BACKGROUND: The successful management of jujube trees depends on maintaining a balance between reproductive and vegetative growth. In general, heading cuts of trunk stimulate rather vegetative growth, but could decrease flower initiation. This study was conducted to establish a heading-back pruning severities at planting in the one year old 'Daeneung' jujube whip nursery tree by investigating vegetative growth and fruit yield. METHODS AND RESULTS: The heading back pruning severity was assigned as 5 different ranges as follow: one bud (TR-10, heading back pruning was 10 cm above the ground), three buds (TR-30), five buds (TR-50), seven buds (TR-70), and nine buds (TR-90) were left of scion. The number of buds on whip trunk was correlated to the central leader length and increment of trunk cross-sectional area, negatively, and to the tree height and canopy volume, positively. The yields in the TR-50 treatment were higher about 2 times than other treatments. The fruit diameter was not significantly different among the treatments. CONCLUSION: These results indicated that the optimum location of heading-back pruning in one year old 'Daeneung' jujube tree was 50 cm above the ground as evaluated by the balance of reproductive and vegetative growth.