Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Scanning electron microscopy of filiform papillae development in Korean native goats (Capra hircus)

  • Lee, Si-Joon (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Cho, Gyu-Hyen (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Mun-Ki (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Chong-Sup (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Won, Chung-Kil (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University)
  • Received : 2018.09.06
  • Accepted : 2018.11.09
  • Published : 2018.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to investigate morphological development of filiform papillae (FP) in Korean native goats by using scanning electron microscopy. Tongues were removed from goat fetuses (days 60, 90, and 120), neonates, and juveniles (days 30, 60, 90, 120, 150, and 180 after birth). During the prenatal period, primordia of FP appeared at fetal day 60 and were observed to be developed at day 90. At fetal day 120, the FP were observed like flower leaves of a double flower bud. In neonates, FP were shaped like an obliquely sectioned cylinder with secondary papillae irregularly arranged in a saw blade-like manner. In 60-day-old juvenile goats, the FP were densely distributed at the inner base of 1/3-1/2 degrees. In 90-, 120-, and 150-day-old goats, FP were compacted at the inner base of 1/2-2/3, 3/4, and 4/5 degrees, respectively. In 180-day-old goats, FP were found to be completely compacted on the inner surface with complete morphogenesis. Microridges, microplicae, and micropits were well-developed on the epithelial surface of lingual papillae from embryonic day 120 to juvenile day 180. These results indicate that FP of goats have different shapes and sizes during development both before and after birth.

Keywords

TSSOBU_2018_v58n4_171_f0001.png 이미지

Fig. 1. Scanning electron micrographs showing prenatal filiform papillae (FP) of Korean native goats. (A) Undeveloped primordia of FP appeared in 60-day-old fetus (embryonic day [E] 60). (B) High magnification view on the surface of FP in E60. (C) Developing primordia of FP are seen in 90-day-old fetus. (D) FP shaped like a double flower bud in 120-day-old fetus (E120). (E) High magnification view on the surface of FP in E120. (F) FP have empty inside and irregularly arranged saw blade-like processes in neonates. FUP, fungiform papillae; MP, microplicae. Scale bars = 100 μm (A, B, D and F), 1 μm (B and E).

TSSOBU_2018_v58n4_171_f0002.png 이미지

Fig. 2. Scanning electron micrographs showing postnatal FP from juveniles of Korean native goats. (A) FP have big and long primary papillae with narrow and low secondary papillae in 30-day-old goat. (B) FP of 60-day-old goat and (C) FP of 90-dayold goat showed well-developed secondary papillae. (D) FP of 120-day-old goat and (E) FP of 150-day-old goat had many small peaked papillae processes. (F) FP of 180-day-old goat had completed morphogenesis. Scale bars = 100 μm.

TSSOBU_2018_v58n4_171_f0003.png 이미지

Fig. 3. High magnification view on the surface of postnatal developing FP in Korean native goats. (A) Many MP are developed on the surface of FP in 30-day-old goat. (B) MP are long and straight shaped like thread while microridge (MR) is observed in 60-day-old goat. (C) MP and MR are well-developed in 90-day-old goat. (D) The surface epithelium of FP has straight MP in 180-day-old goat. Scale bars = 1 μm.

TSSOBU_2018_v58n4_171_f0004.png 이미지

Fig. 4. Scanning electron microscopy measurements of the length of FP. Each height and oblique length were measured from postnatal day 0 (P0) to 180-day-old Korean native goats (P180). There were no statistically significant differences in size of FP during the next 30 days (p > 0.05).

References

  1. Boshell JL, Wilborn WH, Singh BB. A correlative light microscopic, transmission and scanning electron microscopic study of the dorsum of human tongue. Scan Electron Microsc 1980, 505-510.
  2. Chamorro CA, de Paz P, Sandoval J, Fernandez JG. Comparative scanning electron-microscopic study of the lingual papillae in two species of domestic mammals (Equus caballus and Bos taurus). I. Gustatory papillae. Acta Anat (Basel) 1986, 125, 83-87. https://doi.org/10.1159/000146141
  3. Cho G, Kim M, Lee S, Kim C, Won C. Scanning electron microscopic study of the developing vallate papillae in the Korean native goat (Capra hircus). Dev Reprod 2016, 20, 283-288. https://doi.org/10.12717/DR.2016.20.4.283
  4. Davies RO, Kare MR, Cagan RH. Distribution of taste buds on fungiform and circumvallate papillae of bovine tongue. Anat Rec 1979, 195, 443-446. https://doi.org/10.1002/ar.1091950304
  5. de Paz Cabello P, Chamorro CA, Sandoval J, Fernandez M. Comparative scanning electron-microscopic study of the lingual papillae in two species of domestic mammals (Equus caballus and Bos taurus). II. Mechanical papillae. Acta Anat (Basel) 1988, 132, 120-123. https://doi.org/10.1159/000146562
  6. Eerdunchaolu, Takehana K, Yamamoto E, Kobayashi A, Cao G, Baiyin, Ueda H, Tangkawattana P. Characteristics of dorsal lingual papillae of the Bacterian Camel (Camelus bactrianus). Anat Histol Embryol 2001, 30, 147-151. https://doi.org/10.1111/j.1439-0264.2001.t01-1-0317.x
  7. Emura S, Hayakawa D, Chen H, Shoumura S. Morphology of the lingual papillae in the tiger. Okajimas Folia Anat Jpn 2004, 81, 39-43. https://doi.org/10.2535/ofaj.81.39
  8. Emura S, Okumura T, Chen H. Morphology of the lingual papillae in the fishing cat. Okajimas Folia Anat Jpn 2014, 90, 79-83. https://doi.org/10.2535/ofaj.90.79
  9. Emura S, Tamada A, Hayakawa D, Chen H, Shoumura S. Morphology of the dorsal lingual papillae in the barbary sheep, Ammotragus lervia. Okajimas Folia Anat Jpn 2000, 77, 39-45. https://doi.org/10.2535/ofaj1936.77.2-3_39
  10. Erdo an S, Villar S, Konig HE, Perez W. Papillary architecture of the lingual surface in the puma (Puma concolor). Anat Histol Embryol 2018, 47, 51-57. https://doi.org/10.1111/ahe.12323
  11. Furubayashi R, Sato E, Ishibashi T. [Histological pattern of the tongue in the Japanese weasels. Mastela itatsi, with special reference to the morphology and distribution of papillae, taste buds and lingual gland]. Kaibogaku Zasshi 1989, 64, 210-214. Japanese.
  12. Iwasaki S, Yoshizawa H, Kawahara I. Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the mouse. Acta Anat (Basel) 1996, 157, 41-52. https://doi.org/10.1159/000147865
  13. Iwasaki S, Yoshizawa H, Kawahara I. Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the rat. Anat Rec 1997, 247, 528-541. https://doi.org/10.1002/(SICI)1097-0185(199704)247:4<528::AID-AR12>3.0.CO;2-R
  14. Krause WJ, Cutts JH. Morphological observations on papillae of the opossum tongue. Acta Anat (Basel) 1982, 113, 159-168. https://doi.org/10.1159/000145551
  15. Kumar P, Kumar S, Singh Y. Tongue papillae in goat: a scanning electron-microscopic study. Anat Histol Embryol 1998, 27, 355-357. https://doi.org/10.1111/j.1439-0264.1998.tb00207.x
  16. Kumar S, Bate LA. Scanning electron microscopy of the tongue papillae in the pig (Sus scrofa). Microsc Res Tech 2004, 63, 253-258. https://doi.org/10.1002/jemt.20036
  17. Lee HS, Lee I, Kang T. Immunohistochemical studies on the distribution of neuropeptides in the tongue of Korean native goat. Korean J Vet Res 1996, 36, 265-276.
  18. Ojima K. Functional role and angioarchitectural arrangement of the filiform and fungiform papillae on the medial-dorsal surface of the beagle dog tongue. Ann Anat 2001, 183, 325-329. https://doi.org/10.1016/S0940-9602(01)80172-2
  19. Singh BB, Boshell JL, Steflik DE, McKinney RV Jr. A correlative light microscopic, scanning and transmission electron microscopic study of the dog tongue filiform papillae. Scan Electron Microsc 1980, 511-515.
  20. Witt M, Reutter K. Scanning electron microscopical studies of developing gustatory papillae in humans. Chem Senses 1997, 22, 601-612. https://doi.org/10.1093/chemse/22.6.601