• Microbiology and Biotechnology Letters
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• v.32 no.2
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• pp.184-189
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• 2004

Rice wine cake (RWC) is the solid waste obtained after rice wine fermentation. For the mass production of the spores of yeast Saccharomyces from RWC, the optimum pretreatment condition of RWC, the optimum composition of culture medium, and the optimum culture condition were examined. For sporulation, yeast cells were grown in the pre sporulation medium (PSM), transferred into sporulation medium (SM) containing 1 % potassium acetate, and incubated in a rotary shaking incubator at $25^{\circ}C$ for 4 days. The supernatant of the mixture of RWC and water was used as the presporulation medium (PSM). The optimum temperature and time for the pre-incubation of the mixture of RWC and water (1:2) to obtain maximum sporulation yield were $V^{\circ}C$ and 24 hr, respectively, and optimum culture time in PSM was 48 hr. Using these optimum conditions, the asci number obtained was 0.72$ 1.06${\times}$10^{8}$$m\ell$. The addition of wheat coat koji into SM increased the final number of asci to beTEX>$10^{8}$ $m\ell$. Spores were formed in the SM with the initial pH of 7-11, but no spores were formed in the SM with the initial pH of 5. To save the time and effort to pretreat the RWC, 2% and 0.5% RWC without any pretreatment were directly added into PSM containing 1 % brown sugar and SM, respectively, and the maximum asci number of $1.27${\times}$10^{8}$ /$m\ell$ was obtained.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.21-21
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• 2014

A edible mushroom, Hypsizygus marmoreus is commercially cultivated in Northeast Asia. Japan's annual production is 110,000ton or more. Since 2002, cultivation is expanded in Korea. To investigate the morphological, cultural and microscopic characteristics of Hypsizygus marmoreus, 109 isolates were collected from Korea and other countries. Clamp connection, chlamydospore and arthrospore were present in all tested isolates of H. marmoreus except HYM-002 and HYM-004. Also pilealtrama, gilltrama, basidia, basidiospore and cystidia of fruiting body were no difference among the isolates in the present investigation. Morphological characteristics of fruiting body was that color of pileus was brown and white, irregular as marble, the average size 12~22mm and stipes was $46{\sim}91{\times}6{\sim}10mm$. Isolates HYM-031, HYM-047 and HYM-109 formed grayish-brown pileus with a faint pattern. Molecular analysis with RAPD and ITS rDNA sequence analysis were also performed to check the genetic relationships among H. marmoreus isolates. Based on the RAPD analysis using the URP-PCR, all isolates of H. marmoreus were clustered into large 3 groups but more than 90% showed high similarity. In addition, morphological and geographical differences have been classified as an independent cluster. The brown and white strains enclosed in same cluster. So genetically no significance difference was observed between these two strains. ITS gene sequences of 16 selected isolates which were 640 bp long, were aligned and compared. The similarity in ITS sequence was 94.8 to 99.1% among tested isolates and the H. marmoreus isolates in GeneBank. In conclusion the tested isolates were H. marmoreus. Morphological and molecular observations proved that all tested isolates were belonging to H. marmoreus. For the stable artificial cultivation, composition of optimum media, mature period and light condition were established. Optimal formula of artificial cultivation medium was Douglas sawdust: corn cob: soybean meal: wheat bran = 40:30:15:15. In addition, 7% rice bran and 3% yellow sucrose was the most effective composition for spawn's liquid medium. For the maturation of the isolates was favorable for growing for 20 to 30 days at $25^{\circ}C$ and the LED lights in mixture of white and blue was good for growth period. For effective growth, the temperature, humidity and aeration control in every step was important.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.4
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• pp.267-274
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• 2022

The effect of year, varieties, nitrogen application, and transplant time were examined in relation to rice of protein. An experiment was conducted using 12 rice varieties to investigate the effect of management and weather conditions on brown rice protein of during the filling stage. The transplanting time was set to be three groups including early, medium, and late timing. The nitrogen application was set to be 0 N kg / 10a, 9N kg / 10a and 18 N kg / 10a to examine the effect of fertilizer management on protein content. Field experiments were conducted in three growing seasons including 2019, 2020, and 2021. The brown rice of protein content were 5.7%, 5.9%, and 6.6% under early, medium, and late transplanting time conditions, respectively. The protein content differ by variety. For example, Chucheong, Hopum, Ilpum, Mipum, Odae, Saenuri, and Saeilmi had more than 6.1%, and Chindeul, Shindongjin, Samkwang, Unkwang, Younhojinmi were less than 6.1%. Nitrogen content was 5.7% for 0kgN /10a, 6.1% for 9kgN /10a, and 6.8% for 18kgN /10a. The contribution of the characteristics to the protein content was highest in nitrogen content (38.8%), followed by transplanting time (13.7%), variety (8.2%), and year (3.5%). The average temperature for 20 days after heading time was the highest (9.3%), followed by sunshine duration (3.9%) and solar radiation (3.5%). Our results revealed that brown rice protein content was determined to be affected by changes in average temperature, sunshine duration and solar radiation for 20 days after heading time. This suggested that assessment of temperature and solar radiation after heading time would indicate the degree of rice quality in terms of protein.

• Mycobiology
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• v.34 no.1
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• pp.30-33
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• 2006

To screen additives and their mixed ratio suitable for the mycelial growth and fruiting body formation of Oudemansiella radicata in the oak sawdust, additives such as rice bran, fermented soybean powder and wheat bran were used. Generally, the mycelial growth of O. radicata has been stable on oak sawdust mixed with rice bran of $5{\sim}20%$. In case that O. radicata was cultured for about 30 days at $22{\pm}1^{\circ}C$ under the illumination (350 lux) of 12 hours and moisture condition of $90{\pm}5%$, the primordia have been formed gradually from red-brown crusts covering the surface of oak sawdust media. Based on the experimental results from 9 strains of O. radicata, fruiting bodies were produced widely on oak sawdust medium mixed with rice bran of 5 to 30%. Even though fruiting bodies of O. radicata have been produced well on oak sawdust media mixed with rice bran, fruiting bodies of O. radicata were produced intensively on oak sawdust media mixed with rice bran of 10%. Therefore, this result will provide a basic information for commercial production of fruiting body of wild O. radicata. This result is the first report associated with an artificial fruiting body formation of O. radicata in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.379-385
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• 2006

This experiment was carried out to investigate the optimum planting density in low fertilizing cultivation of machine transplanting in rice field of Honam Agricultural Research Institute, NICS for $2004{\sim}2005$. Sobibyeo which belongs to medium maturing variety and Nampyeongbyeo which belongs to medium-late maturing variety were transplanted on May 30. In this experiment, there was no significant difference in heading date between planting density and nitrogen fertilization rate, and heading dates were August 8 in Sobibyeo, and August 14 in Nampyeongbyeo respectively. In relation to lodging character, lodging Index was high where the nitrogen fertilization rate and planting density were high. As planting density increases, panicle number per $m^{2}$ increased irrespective of nitrogen fertilization rate. When nitrogen was 6 kg/10a, rice yield of Sobibyeo was more where planting density was 90 hill per $3.3m^{2}$, and that of Nampyeongbyeo was more where planting density was 80 hill per $3.3m^{2}$. When nitrogen was 9 kg/10a, rice yield of Sobibyeo was more where planting density was 100 hill per $3.3m^{2}$, and that of Nampyeongbyeo was more where planting density was 110 hill per $3.3m^{2}$. Head rice rate of brown rice was higher when planting density increased, and was higher at 6 kg/10a nitrogen rate than 9 kg/10a nitrogen rate in all varieties.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.513-518
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• 2011

'Hongjinju', the reddish brown coloured rice variety, was developed by the rice breeding team of National Institute of Crop Science, RDA during the period from 1990 to 2006 and released in 2007. The cultivar was derived from a cross between Suwon 383, and $SR18164F_2$, a reddish brown pigmented line. 'Hongjinju' is medium maturity with 133 days of growth duration from seeding to heading and is japonica-type with about 82 cm in culm length. 'Hongjinju' has slightly less number of tillers per hill and more spikelets of panicles than those of 'Heugjinjubyeo'. It is susceptible to leaf blast, other disease and insect pests. It shows intermediate germination ratio at low temperature and similar tolerance to leaf discolor at seeding stage and heading-delay but it is resistant to spikelets-sterility type of cold damage at maturing stage compared with 'Heugjinjubyeo'. The yield performance of this variety in brown rice is about 5.07 MT/ha and is adaptable to the central and southern plain areas of Korea.

• Korean Journal of Food Science and Technology
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• v.11 no.2
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• pp.122-125
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• 1979

Degermed brown rice of Akibare (short grain) and Milyang 23 (medium grain) was abraded fiveconsecutive times to remove outer $5{\sim}6%$ of the kernel per milling. Samples were analyzed for calcium, phosphorus, iron, thiamine and riboflavin. Milled fraction I (about $5{\sim}6%$ of the kernel) contained 8 times as much calcium and phosphorus as did the original kernel; iron, $4{\sim}5$; thiamine, 3; and riboflavin, 4. Contents of fraction I were much greater than those in the residual kernel; 18 times as great for calcium; $32{\sim}36$ times for phosphorus; $5{\sim}10$ times for iron 5 times for thiamine; and $19{\sim}30$ times for riboflavin. Milyang 23 showed a steeper concentration gradient of calcium and riboflavin, but more even distribution of iron than did Akibare. There were no significant differences in phosphorus and thiamine gradients between the two rices.

• Korean Journal of Organic Agriculture
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• v.11 no.3
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• pp.75-90
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• 2003

A total of 192 Korean landraces were investigated for the comparison of each other as useful germplasm to organic farming and examined correlation between quantitative traits. Almost Korean landraces were classified into medium-heading variety and average days from seeding to heading was 111.1 days. Plant height and culm length were longer about 20∼40cm than general Japonica cultivar. Average width of leaf blade and average length of panicle were 1.3cm and 22.4cm, respectively, Average number of panicles per plant was 10.0 and almost varieties showed low tilling habit. Average length/width ratio of brown rice was 1.7 and several varieties were long grain type and average 1,000 grain weight was 21.6g. In correlation coefficient among quantitative traits, positive significant correlations were observed between culm length and leaf width, days from seeding to heading, but negative correlations were observed between days from seeding to heading and 1000 grain weight. 27.1% of Korean landraces showed weak and lodging degree in culm strength and 3.6% of total varieties showed purple margin on leaf blade and leaf sheath. In leaf blade angle and flag leaf angle, erect type was 46.9% and 10.9%, respectively. 24.0% of total varieties showed the slow and late degree of leaf senescence. The rate of awned type, waxy type and brown seed coat were 74.5%, 20.3% and 4.2%, respectively. The color of stigma, spikelet, apiculus and awn showed diverse color such as white, brown, red and purple.

• Journal of Life Science
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• v.17 no.8 s.88
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• pp.1141-1146
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• 2007

This study investigated the effects on the biological activities of germinated brown rice soaked in mycelial culture broth of Phellinus linteus. The level of free amino acid was higher in the GBRP extract than those of BR and GBR. The major free amino acids were alanine, valine, isoleucine and methionine in both extracts. The level of ${\gamma}$-aminobutyric acid (GABA) was also increased significantly in the GBR and GBRP. Antioxidant activities of methanol extract of BR, GBR and GBRP were measured by using DPPH radical scavenging and SOD-like activity. Antioxidant activities showed the highest level of 83% and 76% when 100 mg/ml GBR and GBRP, respectively. Stimulation of the macrophages RAW264.7 cells with lipopolysaccharide (LPS) resulted in increased production of nitric oxide (NO) in the medium. However, the methanol extract of GBR and GBRP showed marked inhibition of NO synthesis in a does-dependant manner. These results showed that GBR and GBRP were significant role for activation of immune system in the pathogenesis of inflammatory diseases.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.136.1-136
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• 2003

Bacterial grain rot by Burkholderia gluae cause severe damage in seedling and grain of rice after heading season. This seed-borne pathogen play a role as first infection agent that could be cause disease following cropping season. Until now the direct isolation of the bacteria has some trouble by interference of other bacteria existed inside seed. This study established convenient identification method as simple isolation with KB medium from seed showing symptom and using PCR identification. By this isolation method, B. glumae was isolated from 40 to 50％ in brown rice and inner hull, however, there were saprophytic bacteria and fungi outer hull. In PCR identification with Ogf4 and Ogr3 primer to these 25 isolates, the amplified products were presented in all of the collections but not in 10 saprophytic germs. The isolation rate was constant to 3 months stored seeds. This result provide a rapid and convenient isolation and identification of B. glumae.