• Journal of Mushroom
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• v.18 no.2
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• pp.145-150
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• 2020

A new cultivar, 'Bambithyang', was bred from monokaryotic strains of Sanbaekhyang and SANJO 707ho using the mono-mono hybridization method. Its incubation period was determined to be 100 days and its optimum temperature for fruit body flushing, 11-20℃. The mushrooms had a convex cap (diameter, 69.6 mm; thickness, 15.2 mm) and stipes in their center. The color of their upper sides was chestnut brown; their gills were arranged in a ripple style and had medium density. Their scales were cream-colored and fully distributed; the stipes were cream-colored and cylindrical (thick upper part). They also had cream colored fluffs and sporadic fruiting bodies. Zone lines were present when they were cultivated with mother and father varieties. Their stipe lengths and caps were 9% shorter and 16.9% thicker, respectively, than those of Sanbaekhyang.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.10
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• pp.446-451
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• 2019

This study investigated the application of artificial insemination and pregnancy diagnosis kit for Korean native black goats. Semen was collected by electrical ejaculation, followed by semen analysis and artificial insemination in three goat strains (Dangjin, Jangsu and Tongyoung). Pregnancy was confirmed using a cow pregnancy test kit (IDEXX Rapid Visual Pregnancy Test kit) and ultrasound diagnosis. Analysis revealed that semen collected from male Korean native black goats by electrical ejaculation was about 1~1.5 ml in volume, $18{\sim}25{\times}10^8/ml$ concentration, and having > 97% motility. Furthermore, confirmation of pregnancy by pregnancy test kit and ultrasound diagnosis after artificial insemination were similar. In addition, the efficiency of pregnancy was 20~40% for all three strains: Tongyoung was the highest with 44%, followed by Dangjin (%), and Jangsu (20%). This study determines the artificial fertilization efficiency and the feasibility of using a cow pregnancy test kit for early pregnancy diagnosis in Korean native black goats. Although further research is required for validation, the results of the current study contribute to the breeding and improvement of Korean native black goat in research institutions as well as in general farms.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.1
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• pp.15-22
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• 1993

Korean local collected strains of perilla were analyzed on their protein and amino-acid contents in the seed. The strains analyzed were 80 of which grown in the experimental field of Crop Experiment Station at Suwon in 1991. This experiment was performed to obtain the basic informations on the varietal differences of protein and amino-acid contents and further applied in the breeding project for quality improvement of perilla. The seed protein contents of 80 perilla varieties ranged from 17.9% to 24.6% and the average content was 24.6%. There were no differences in protein contents according to the maturity and 1,000 seed weight, but differed by the seed coat color showing 1.4% higher content in light gray than that of drak brown, which may suggest that the seed coat color could be used as a marker gene for breeding high protein varieties. While the total essential amino-acid contents of collected perilla ranged from 36.41% to 44.85%, the mean of 40.55%, the non-essential amino-acid contents was 47.23%～60.01% with the mean of 55.29%. Moreover, there was high positive correlation between the essential amino-acid contents and seed coat color. The essential amino-acids of perilla has an exceptionally high content of arginine, leucine, but methionine indeed being almost marginal. Of the essential amino-acids, lysine, methionine and valine were contained higher compared with the FAO recommendation level.

• Journal of Practical Agriculture & Fisheries Research
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• v.19 no.1
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• pp.83-90
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• 2017

In order to develop the culture material for breeding of Lentinula edodes, dikaryotic and monokaryotic mycelia were cultured in agar and sawdust medium. The cultivars were used Sanjo No. 701, Sanjo No. 705, Chamaram and Chuje No. 2 in this study. The mycelial growth of the cultivars were highest in PDA except for Sanjo No. 705, but Sanjo No. 705 showed the best mycelial growth in MCM. Regardless of the cultivars, the mycelial growth showed the most stable in MEA. There was no significant difference in mycelial growth among the cultivars in sawdust medium, but it was about 10% faster than that of Sanjo No. 701 and 705. Monokaryotic strains of Sanjo No. 701 and Chamaram showed less mycelial growth than dikaryotic mycelium. Dikaryotic mycelium of Chamaram showed better mycelial growth than that of Sanjo No. 701, but monokaryotic mycelium of Chamaram showed lower mycelial growth than Sanjo No. 701. The selected monokaryotic mycelium has a wide varience of mycelial growth, and the morphologies of the colonies are very diverse, so those are presumed that wide variences of monokaryotic are selected in genetically, and these monokaryotic mycelium are expected to be a good breeding materials.

• Journal of Mushroom
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• v.19 no.3
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• pp.251-255
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• 2021

The competitiveness of the mushroom industry can be increased by diversifying the domestic Lentinula edodes cultivar. Therefore, 'Chungheung 1ho' was cultivated by hybrid breeding method using strain 'E140025,' which was collected by the Chungheung Mushroom Farming Association. Regarding the morphological characteristics of the fruit, the cap of 'Chungheung 1ho' was smaller than that of the control cultivar, whereas, its stem was longer and thicker than that of the control cultivar. In addition, the weight of the individual fruits of the new cultivar was heavier than that of the control cultivar. The period for primordia formation was 24 days for the new cultivar, which was 16-20 days later than that of the control cultivar. Furthermore, it was found that the patterns of the PCR amplification band using six URP primers were different for 'Chungheung 1ho' than those of the mating strains.

• Journal of Life Science
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• v.23 no.10
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• pp.1192-1198
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• 2013

To improve yeast strains for bioethanol production, yeasts with ethanol tolerance, thermotolerance, and ${\beta}$-1,3-glucanase activity were bred using yeast genome shuffling. Saccharomyces cerevisiae $BY4742{\Delta}exg1$/pAInu-exgA, which has extracellular ${\beta}$-1,3-glucanase activity, and the Aspergillus oryzae and S. cerevisiae YKY020 strains, which exhibit ethanol tolerance and thermotolerance, were fused by yeast protoplast fusion. Following cell fusion, four candidate cells (No. 3, 9, 11, and 12 strains) showing thermotolerance at $40^{\circ}C$ were selected, and their ethanol tolerance (7% ethanol concentration) and ${\beta}$-1,3-glucanase activity were subsequently analyzed. All the phenotypes of the two parent cells were simultaneously expressed in one (No. 11) of the four candidate cells, and this strain was called BYK-F11. The BYK-F11 fused cell showed enhanced cell growth, ethanol tolerance, ${\beta}$-1,3-glucanase activity, and ethanol productivity compared with the $BY4742{\Delta}exg1$/pAInu-exgA and YKY020 strains. The results prove that a new yeast strain with different characters and the same mating type can be easily bred by protoplast fusion of yeasts.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.582-588
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• 2001

To control the growth of Salmonella on raw chicken supplied in food service, they were isolated and analysed for their physiological characteristics. Total viable microbe counts under the skin of the chicken amounted to 10% of numbers on their skin. Fifty one of Salmonella were isolated and identified from 75 chicken carcass samples. About 70% of the isolates showed resistance to more than four antibiotics, which indicated very high resistance among the strains ever reported in Korea. Lactic acid and trisodium phosphate(TSP) as sanitizing agent were applied to the Salmonella spp. isolates by agar diffusion method. The resistance by isolates to those sanitizing agents was compared with type strains of S. typhimurium ATCC 14028, S. typhimurium ATCC 12023, S. heidelberg and S. enteritidis. The isolates showed similar or less resistance to the type strains as for the lactic acid. However, as for trisodium phosphate known recently as a good sanitizer, 50% of the isolates showed more resistant compared with the type strains. Also, the treatment time and regeneration medium affected significantly the resistance of the isolates, which indicates that more careful test might be needed for TSP's practical application to raw chicken. Therefore, From the results, it is suggested that antibiotic treatment during breeding broiler and contamination after slaughtering should be minimized along with careful use of sanitizing agent in order to ensure more safe raw chicken supply.

• Korean Journal of Poultry Science
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• v.20 no.1
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• pp.1-9
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• 1993

This study carried out to build up new synthetic egg production lines which had sex-linked gene for feather color sexing and also superior combining ability for producing the best commercial chicks. The closed flock breeding method was utilized to improve the general performances in the first experiment and combining ability for heterosis was tested for new synthetic line in the second experimental year. In order to test for the egg production ability in cross breeds synthetic lines, the crossing of B$\times$4 B$\times$C, two imported strains and two domestic strains as controls were compared for the general performances. There was on difference in mortality, body weight to 56 weeks of age. Sexual maturity was delayed about 10 days by comparing with other reports, except 153 days of the Manina White, but no difference among mating systems in this experiment. The hen housed egg production in B$\times$A, B$\times$C was 186.3, 191.3 respectively and it was better than the other controls, except ISA imported lines. The hen-day egg production of B$\times$A, B$\times$C was better than other controls, with 75.7%, 76.8% respectively. In the average egg weight, the B$\times$C cross breed was highest with 64.5g. As the sex of hatching chicks was identified by difference of feather color, the genetic composition of synthetic lines must be homogenized. The feather color of female chicks was brown and that of male was silver (99%), In conclusion, the egg production ability of B$\times$A, B$\times$C cross breeds was superior to the imported and domestic lines. Therefore, it suggest that the synthetic lines with sex-linked gene might be utilized for improving egg production performances.

• The Plant Pathology Journal
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• v.26 no.1
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• pp.93-98
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• 2010

A virus isolate causing symptoms of yellow mosaic, fern leaves, malformation and plant necrosis on Rudbeckia bicolor was prevalent around Pyeongchang area in Korea. The causal virus was identified as Cucumber mosaic virus (CMV) using characteristics from biological, serological and molecular analyses and named as CMV-Rb. CMV-Rb caused mosaic on Nicotiana benthamiana, N. tabacum, Capsicum annuum, and Lycopersicon esculentum. However, typical local lesions did not develop on inoculated Pisum sativum, Cucurbita moschata, Datura stramonium and Tetragonia expansa plants. Full-length genome sequences of CMV-Rb RNAs 1, 2 and 3 were obtained using 12 primer pairs by RT-PCR analysis. The genome of CMV-Rb RNA segments 1, 2, and 3 consists of 3363nt, 3049nt, and 2214nt in length, respectively. In order to ascertain their taxonomic identity, nucleotide and the deduced amino acid sequence analyses RNAs 1, 2 and 3 of CMV-Rb isolates were conducted with previously reported sequences of CMV strains and/or isolates. CMV-Rb RNAs showed about 90 to 99% sequence identity to those of subgroup I strains suggesting that CMV-Rb is more closely related to CMV isolates belong to subgroup I. To our knowledge, this is the first report of CMV on Rudbeckia bicolor in Korea.

• Korean Journal of Veterinary Research
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• v.54 no.1
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• pp.39-48
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• 2014

The prevalence of thermophilic Campylobacter (C.) spp. in stray, breeding, and household dogs was 25.2, 12.0, and 8.8%, respectively. C. jejuni and C. upsaliensis were the predominant Campylobacter spp. from household dogs. cdtA, cdtB, and cdtC were detected by PCR in all isolates. Despite the high cytolethal distending toxin (CDT) gene prevalence, only 26 (31%) C. jejuni strains and one (15.3%) C. coli strain showed evidence of CDT production in HEp-2 cell cytotoxicity assays. Virulence-associated genes detected in the C. jejuni and C. coli isolates were cadF, dnaJ, flaA, racR, ciaB, iamA, pldA, virB11, ceuE, and docC. cadF, dnaJ, flaA, and ceuE were found in all C. jejuni and C. coli isolates. When detecting Guillain-Barr$\acute{e}$ syndrome-associated genes (galE, cgtB, and wlaN), galE was identified in all isolates. However, cgtB and wlaN were more prevalent in C. jejuni isolates from humans than those from dogs. Adherence and invasion abilities of the C. jejuni and C. coli strains were tested in INT-407 cells. A considerable correlation (adjusted $R^2$= 0.678) existed between adherence and invasion activities of the Campylobacter spp. isolates.