Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Application of artificial insemination and pregnancy diagnosis kit for Korea native black goats

한국 재래흑염소의 계통별 인공수정과 임신진단 키트의 활용

  • Kim, Kwan-Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Jinwook (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Jeon, Dayeon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sung-Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Seungchang (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sang-Hoon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 김관우 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이진욱 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 전다연 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이성수 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김승창 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이상훈 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2019.08.13
  • Accepted : 2019.10.04
  • Published : 2019.10.31
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Abstract

This study investigated the application of artificial insemination and pregnancy diagnosis kit for Korean native black goats. Semen was collected by electrical ejaculation, followed by semen analysis and artificial insemination in three goat strains (Dangjin, Jangsu and Tongyoung). Pregnancy was confirmed using a cow pregnancy test kit (IDEXX Rapid Visual Pregnancy Test kit) and ultrasound diagnosis. Analysis revealed that semen collected from male Korean native black goats by electrical ejaculation was about 1~1.5 ml in volume, $18{\sim}25{\times}10^8/ml$ concentration, and having > 97% motility. Furthermore, confirmation of pregnancy by pregnancy test kit and ultrasound diagnosis after artificial insemination were similar. In addition, the efficiency of pregnancy was 20~40% for all three strains: Tongyoung was the highest with 44%, followed by Dangjin (%), and Jangsu (20%). This study determines the artificial fertilization efficiency and the feasibility of using a cow pregnancy test kit for early pregnancy diagnosis in Korean native black goats. Although further research is required for validation, the results of the current study contribute to the breeding and improvement of Korean native black goat in research institutions as well as in general farms.

본 연구의 목적은 인공수정 기법을 이용한 한국 재래흑염소 계통별 인공수정 수태율을 알아보고자 정액의 성상, 수태율 및 분만율을 조사하고, 임신진단을 위한 소의 임신진단 키트의 활용 가능성을 알아보는 것이다. 재래흑염소에서 전기자극을 통해 정액을 채취하여 3계통(당진, 장수, 통영)별 정액분석과 발정동기화 된 암컷에 인공수정을 실시하여 계통별 번식능력을 조사하였다. 인공수정된 암컷에서는 소의 임신진단 키트(IDEXX Rapid Visual Pregnancy Test kit)와 초음파진단으로 임신을 확인하였다. 그 결과 개통별 정액채취량은 1~1.5 ml 내외였으며, 정액농도는 $18{\sim}25{\times}10^8/ml$, 정액 채취 직후 정자활력은 97% 이상 높게 나타났다. 그리고 임신진단의 결과에서 소의 임신진단 키트와 초음파진단 모두 동일한 결과를 보였으며, 계통별 인공수정 수태율의 효율은 20~44%로, 통영계통이 44%로 가장 높게 나타났고 장수계통이 20%로 인공수정 수태율이 낮게 나타났다. 본 연구 결과를 통해 한국 재래흑염소의 계통별 인공수정 효율을 알 수 있었고, 조기 임신진단을 위한 소 임신진단 키트의 활용가능성을 확인할 수 있었다. 본 연구 결과는 재래흑염소 증식과 개량에 기여할 수 있을 것으로 사료된다.

Keywords

References

  1. M. A. Zeder, B. Hesse, "The initial domestication of goats (Capra hircus ) in the Zagros mountains 10,000 years ago", Science, 287:2254-2257, 2000. DOI: https://doi.org/10.1126/science.287.5461.2254
  2. H. B. Soon, "Effects of the Grazing and Barn Feeding System on Growth Performance and Carcass Characteristics in Korean Black Goats", Journal of Agriculture & Life Science, 48:123-131, 2014. DOI: https://doi.org/10.14397/jals.2014.48.2.123
  3. S. S. Lee, S. W. Kim, K. W. Kim, H. Y. Cho, C. Y. Cho, S. H. Yeon, T. J. Choi, "Growth Curve Parameters for Body Weight by Sex in Korean Native Goat", Annals of Animal Resource Sciences, 27:152-158, 2016. DOI: https://doi.org/10.12718/AARS.2015.26.2.110
  4. H. B. Song, I. H. Jo, M. J. Jun, Y. K. Park, K. C. Hong, N. C. Park, J. C. Do, H. S. Lim, "Study on the increasing method of income in the goat farmers", Daegu Univ. Press, 47-50, 1999.
  5. B. K. Kim, J. H. Lee, D. J. Jung, K. H. Cho, E. G. Hwang, M. S. Kim, "Effects of Feeding Herb Resources Powder on Meat Quality and Sensory Properties in Korean Native Black Goat", Food Science of Animal Resources, 30:811-818, 2010. DOI: https://doi.org/10.5851/kosfa.2010.30.5.811
  6. J. H. Kim, M. J. Byun, Y. G. Ko, S. W. Kim, S. W. Kim, Y. J. Do, M. J. Kim, S. H. Yoon, S. B. Choi, "Phylogenetic Analysis of Korean Native Goats Based on the Mitochondrial Cytochrome b Gene", Journal of Animal Science and Technology, 54:241-246, 2012. DOI: https://doi.org/10.5187/JAST.2012.54.4.241
  7. S. W. Suh, M. J. Byun, Y. S. Kim, M. J. Kim, S. B. Choi, Y. G. Ko, D. H. Kim, H. T. Lim, J. H. Kim, "Analysis of Genetic Diversity and Relationships of Korean Native Goat Populations by Microsatellite Markers", Journal of Life Science, 22:1493-1499, 2012. DOI: https://doi.org/10.5352/JLS.2012.22.11.1493
  8. M. Mellado, R. Valdez, L. M. Lara, J. E. Garcia, "Risk factors involved in conception, abortion, and kidding rates of goats under extensive conditions", Small Ruminant Research, 55:191-198, 2004. DOI: https://doi.org/10.1016/j.smallrumres.2003.10.016
  9. J. F. Nunes, C. C. M. Salgueiro, "Strategies to improve the reproductive efficiency of goats in Brazil", Small Ruminant Research, 98:176-184, 2011. DOI: 10.1016/j.smallrumres.2011.03.036
  10. F. Arrebola, O. Gonzalez, R. Torres, J. Abecia, "Artificial insemination in Payoya goats: factors affecting fertility", Animal production science, 54:356-362, 2014. DOI: https://doi.org/10.1071/AN13138
  11. G. Baril, B. Remy, B. Leboeuf, J. F. Beckers, J. Saumande, "Synchronization of estrus in goats: the relationship between eCG binding in plasma, time of occurrence of estrus and fertility following artificial insemination", Theriogenology, 45:1553-1559, 1996. DOI: https://doi.org/10.1016/0093-691X(96)00123-9
  12. A. Fatet, M. T. Pellicer-Rubio, B. Leboeuf, "Reproductive cycle of goats", Animal Reproduction Science, 124:211-219, 2011. DOI: https://doi.org/10.1016/j.anireprosci.2010.08.029
  13. J. F. Nunes, C. C. M. Salgueiro, "Strategies to improve the reproductive efficiency of goats in Brazil", Small Ruminant Research , 98:176-184, 2011. DOI: https://doi.org/10.1016/j.smallrumres.2011.03.036
  14. F. A. Arrebola, B. Pardo, M. Sanchez, M. D. Lopez, C. C. Perez-Marin, "Factors influencing the succes of an artificial insemination program in Florida goats", Spanish Journal of Agricultural Research, 10:338-344, 2012. DOI: https://doi.org/10.5424/sjar/2012102-223-11
  15. I. V. .Engelanda, E. R. opstada, O. Andresena, L. O. Eikb, "Pregnancy diagnosis in dairy goats using progesterone assay kits and oestrous observation", Animal Reproduction Science, 47(3):237-243, 1997. DOI: https://doi.org/10.1016/S0378-4320(96)01632-6
  16. Y. S. Park, H. S. Hwang, J. W. Woo, N. W. Kim, "Characteristics of semen and coat color distribution of offsprings produced by AI in Korean native striped cattle", Reprod. Dec. Biol., 31(1):43-48, 2007.
  17. J. F. Beckers, P. V. Drion, J. M. Garbayo, Z. Perényi, A. Zarrouk, J. Sulon, B. Remy, O. Szenci, "Pregnancy associated glycoproteins in ruminants: inactive members of the aspartic proteinase family", Acta. Vet. Hung., 47(4):461-9, 1999. DOI: https://doi.org/10.1556/AVet.47.1999.4.6
  18. G. Haugejorden, S. Waage, E. Dahl, K. Karlberg, J. F. Beckers, E. Ropstad, "Pregnancy associated glycoproteins (PAG) in postpartum cows, ewes, goats and their offspring", Theriogenology, 66(8):1976-84, 2006. DOI: https://doi.org/10.1016/j.theriogenology.2006.05.016
  19. U. Tandiya, V. Nagar, V. P. Yadav, I. Ali, M. Gupta, S. S. Dangi, I. Hyder, B. Yadav, M. Bhakat, V. S. Chouhan, F. A. Khan, V. P. Maurya, M. Sarkar, "Temporal changes in pregnancy-associated glycoproteins across different stages of gestation in the Barbari goat", Anim. Reprod. Sci., 142(3-4):141-8, 2013. DOI: https://doi.org/10.1016/j.anireprosci.2013.09.016