• 대한한의학원전학회지
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• 제18권2호통권29호
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• pp.190-204
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• 2005

The purpose of this research is to discover changes of WBC and ANC numbers before and after applying Oriental medicine therapy to blood cancer patients. After that, appropriate music therapy method was well planned and carried for patients. Demonstration and music were conducted according to Ohaeng theory. The oriental music therapy was conducted three hours everyday by listening to music and we made patients participate in playing the instrument for one hour in two times a week. The result was verified in three ways by checking and comparing numbers of WBC, ratio of ANC and New Trophil before and after the experiment. In addition to that verification, we analyzed patients' survey and their response after treatment. The result was that WBC and ANC were efficient as p=0.0419, p=0.0262 each and the ratio of New trophil was not efficient in p=0.999, but partially increased.

• 한국컴퓨터정보학회논문지
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• 제23권4호
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• pp.147-153
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• 2018

In this paper, we propose an efficient WBC 14-Diff classification which performs using the WBC-ResNet-152, a type of CNN model. The main point of view is to use Super-pixel for the segmentation of the image of WBC, and to use ResNet for the classification of WBC. A total of 136,164 blood image samples (224x224) were grouped for image segmentation, training, training verification, and final test performance analysis. Image segmentation using super-pixels have different number of images for each classes, so weighted average was applied and therefore image segmentation error was low at 7.23%. Using the training data-set for training 50 times, and using soft-max classifier, TPR average of 80.3% for the training set of 8,827 images was achieved. Based on this, using verification data-set of 21,437 images, 14-Diff classification TPR average of normal WBCs were at 93.4% and TPR average of abnormal WBCs were at 83.3%. The result and methodology of this research demonstrates the usefulness of artificial intelligence technology in the blood cell image classification field. WBC-ResNet-152 based morphology approach is shown to be meaningful and worthwhile method. And based on stored medical data, in-depth diagnosis and early detection of curable diseases is expected to improve the quality of treatment.

• BMB Reports
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• 제55권7호
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• pp.336-341
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• 2022

Narrowing of arteries supplying blood to the limbs provokes critical hindlimb ischemia (CLI). Although CLI results in irreversible sequelae, such as amputation, few therapeutic options induce the formation of new functional blood vessels. Based on the proangiogenic potentials of stem cells, in this study, it was examined whether a combination of dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (HUVECs) could result in enhanced therapeutic effects of stem cells for CLI compared with those of DPSCs or HUVECs alone. The DPSCs+ HUVECs combination therapy resulted in significantly higher blood flow and lower ischemia damage than DPSCs or HUVECs alone. The improved therapeutic effects in the DPSCs+ HUVECs group were accompanied by a significantly higher number of microvessels in the ischemic tissue than in the other groups. In vitro proliferation and tube formation assay showed that VEGF in the conditioned media of DPSCs induced proliferation and vessel-like tube formation of HUVECs. Altogether, our results demonstrated that the combination of DPSCs and HUVECs had significantly better therapeutic effects on CLI via VEGF-mediated crosstalk. This combinational strategy could be used to develop novel clinical protocols for CLI proangiogenic regenerative treatments.

• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.147-153
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• 1993

A total of 109 heads of horses and ponies from eight horseback riding clubs nearby Central Taiwan were investigated to evaluate the blood parameters and the biochemical compositions of serum for the documentation of clinical pathological diagnosis and exercise physiology. Blood samples were collected from the jugular vein of resting horses. The sex difference in the blood traits were compared both in horses and in ponies. Results shows that total plasma proteins (PP) and hematocrit (PCV) were found higher in male horses than in females (p < 0.05). The sexual effect also exertes a significant influence on the leucocyte (WBC) count, but not on the erythrocyte (RBC) concentration. According to the differential counts of leucocytes, the number of monocytes and lymphocytes was higher in the male pony than that of in male horse. A close relationship Between the erythrocyte sedimention rate (ESR) and the other blood parameters were found especially in PCV, RBC concentration, and plasma protein level. The average ESR observed at 60 minutes were $21.80{\pm}21.87mm$, $39.50{\pm}18.90mm$ and $43.73{\pm}17.89mm$ in stallions, geldings, and mares, respectively. Most of the biochemical components of horse serum detected were distributed in normal ranges, although some of the items show a great variation in such a large sample size.

• Annals of Laboratory Medicine
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• 제38권6호
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• pp.599-603
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• 2018

Frequencies of red blood cell (RBC) blood group antigens differ by ethnicity. Since the number of immigrants is increasing in Korea, RBC antigens should be assessed in children/youths with parents of different ethnicities to ensure safe transfusions. We investigated the frequency of RBC antigens, except for ABO and RhD, in 382 children and youths with parents having Korean and non-Korean ethnicities. Subjects were divided into those with ethnically Korean parents (Korean group; N=252) and those with at least one parent of non-Korean ethnicity (non-Korean group; N=130). The 37 RBC antigens were genotyped using the ID CORE XT system (Progenika Biopharma-Grifols, Bizkaia, Spain). The frequencies of the Rh (E, C, e, $hr^S$, and $hr^B$), Duffy ($Fy^a$), MNS ($Mi^a$), and Cartwright ($Yt^b$) antigens differed significantly between the two groups. Eight and 11 subjects in the Korean and non-Korean groups, respectively, exhibited negative expression of high-frequency antigens, whereas 14 subjects in the non-Korean group showed positive expression of low-frequency antigens. The frequency of RBC antigens has altered alongside demographic changes in Korea and might lead to changes in distribution of RBC antibodies that cause acute or delayed hemolytic transfusion reaction.

• Journal of Chest Surgery
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• 제55권3호
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• pp.214-224
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• 2022

Background: Studies of the prognostic role of circulating tumor cells (CTCs) in early-stage non-small cell lung cancer (NSCLC) are still limited. This study investigated the prognostic power of CTCs from the pulmonary vein (PV), peripheral blood (PB), and bone marrow (BM) for postoperative recurrence in patients who underwent curative resection for NSCLC. Methods: Forty patients who underwent curative resection for NSCLC were enrolled. Before resection, 10-mL samples were obtained of PB from the radial artery, blood from the PV of the lobe containing the tumor, and BM aspirates from the rib. A microfabricated filter was used for CTC enrichment, and immunofluorescence staining was used to identify CTCs. Results: The pathologic stage was stage I in 8 patients (20%), II in 15 (38%), III in 14 (35%), and IV in 3 (8%). The median number of PB-, PV-, and BM-CTCs was 4, 4, and 5, respectively. A time-dependent receiver operating characteristic curve analysis showed that PB-CTCs had excellent predictive value for recurrence-free survival (RFS), with the highest area under the curve at each time point (first, second, and third quartiles of RFS). In a multivariate Cox proportional hazard regression model, PB-CTCs were an independent risk factor for recurrence (hazard ratio, 10.580; 95% confidence interval, 1.637-68.388; p<0.013). Conclusion: The presence of ≥4 PB-CTCs was an independent poor prognostic factor for RFS, and PV-CTCs and PB-CTCs had a positive linear correlation in patients with recurrence.

• The Korean Journal of Physiology and Pharmacology
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• 제20권5호
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• pp.533-538
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• 2016

Angiogenesis plays an essential role in embryo development, tissue repair, inflammatory diseases, and tumor growth. In the present study, we showed that endothelial nitric oxide synthase (eNOS) regulates retinal angiogenesis. Mice that lack eNOS showed growth retardation, and retinal vessel development was significantly delayed. In addition, the number of tip cells and filopodia length were significantly reduced in mice lacking eNOS. Retinal endothelial cell proliferation was significantly blocked in mice lacking eNOS, and EMG-2-induced endothelial cell sprouting was significantly reduced in aortic vessels isolated from eNOS-deficient mice. Finally, pericyte recruitment to endothelial cells and vascular smooth muscle cell coverage to blood vessels were attenuated in mice lacking eNOS. Taken together, we suggest that the endothelial cell function and blood vessel maturation are regulated by eNOS during retinal angiogenesis.

• Radiation Oncology Journal
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• 제14권1호
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• pp.53-59
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• 1996

목적 : 암환자에서 방사선치료에 의한 면역기능의 저하에 대해서는 많은 보고가 되어 있다. 저자들은 방사선치료부위중 비교적 활동성 골수를 많이 포함하고 있는 흥부 및 골반강조사 직후 어느정도 면역력의 저하가 오는지 알아보고자 하였다. 대상 및 방법 : 1995년 1월부터 1995년 4월까지 등록된 61 명의 환자중 48 명을 대상으로 분석 하였다. 이중 흉부(조사문, >$150cm^2$)에 방사선치료를 시행한 환자는 29명이었고 전골반강부에 방사선치료를 시행한 환자는 19명 이었다. 연령분포는 36세에서 73세 였으며 평균 및 중간값 모두 57세 였으며 남녀비는 1.3(27/21)이었다. 환자의 면역기능의 지표는 말초혈액검사에서 전혈구 및 감별혈구계산(CBC with D/C), 간기능검사, 신장기능검사 및 림프구아형검사(CD3, CD4, CD8, CDl6, CD56, CDl9)를 시행 하였으며, 검사시기는 방사선치료 직전과 4500 cGy - 5000 cGy 선량에서 동일 검사를 반복시행 하였으며, 1980cGy에서는 전혈구 및 감별혈구계산만 시행하였다. 결과 : 전체환자의 치료전 백혈구 총수는 7017이었으며 방사선치료직후 평균 4470으로 감소하였다(p=0.0000). 감별혈구계산에서는, 림프구수는 평균 2047 에서 537 로(p=0.0000) 로 감소하였고, 호중구, 호염구세포의 절대수도 통계학적으로 유의한 감소를 보였으나, 단핵세포는 변화가 없었으며, 호산구세포는 오히려 방사선치료후 증가하였으나 통계적인 의의는 없었다. 림프구아형에 대한 검사결과는, 모든아형의 절대수가 통계학적으로 유의한 감소를 보였으며, CD4/CD8비는 치료전 평균 1.09에서 0.99로 감소 하였으나 통계적인 유의성은 없었다. 전체 림프구에 대한 비율의 변화를 보면, B림프구(CD 19)는 감소하였으나, 그외 아형의 비율은 방사선치료후 변화를 보이지 않았다. 혈청면역글로불린은 초기 Ig, G, Ig A, Ig M 모두 정상값보다 눌은 수치였으며, 방사선치료에 따른 변화는 Ig M에서만이 통계적으로 유의한 감소를 보였으며, Ig G, A는유의한 변화가 없었다. 결론 : 흉부나 골반강부위의 방사선치료는 림프구의 급격한 저하를 초래하는 반면 단핵구등은 비교적 잘 유지 되었으며, 호산구는 오히려 증가 됨을 알수 있었으나, 인체의 면역과의 관계를 설명하기 위해서는 림프구의 기능변화가 함께 연구 되어져야 하겠다.

• 대한한방내과학회지
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• 제17권1호
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• pp.8-33
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• 1996

The present experiment was designed to understand the effect of Boganhwan on the cardiovascular system in experimental animals. And thus the Blood pressure, isometric movement of atrium,Mg++-Ca++-ATPasc activity of sarcoplasmin reticulum. liver function, prothrombin time, and changes of blood composition were measured in the presence of Boganhwan. The results obtained here were as following: 1. The blood pressure decreased in the presence of SAMOOLTANG, and the decreasing effect was more stimulated by adding Bangpoong and Ganghwa(Boganhwan) 2. The oral administration of the drug for 7days also demonstrated the decreasing of blood pressure and the effect was stimulated by adding Bangpoong and Kanghwal. 3. The autonomic nerve blocking agents such as atropine and regitine did not demonstrate the effect on the drug action. 4. Boganhwan inhibited the cardiac isometric movement and rate by stimulating the Mg++-Ca++-ATPase activity of the heart sarcoplasmins reticulum. 5. Boganhwan increased the number of red blood cell, hematocrit percentage, hemoglobin concentration, and prothrombin time. 6. The drug stimulated the liver metabolism by stimulating the total ATPase activity. According to the results. Boganhwan demonstrated the decreased blood pressure and it also increased the hemoglobin concentration and the hematocrit percent. These effects stanches the hypertension. anemia, and cerebrovascular accident.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.540-545
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• 2005

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3HB-co-3HV), copolyesters, with 3-hydroxyvalerate (3HV) contents ranging from 17 to 60 mol%, were produced by Alcaligenes sp. MT-16, and their biocompatibility evaluated by the growth of Chinese hamster ovary (CHO) cells and the adsorption of blood proteins and platelets onto their film surfaces. The number of CHO cells that adhered to and grew on these films was higher with increasing 3HV content. In contrast, the tendency for blood proteins and platelets to adhere to the copolyester surfaces significantly decreased with increasing 3HV content. Examination of the surface morphology using atomic force microscopy revealed that the surface roughness was an important factor in determining the biocompatibility of theses copolyesters. The results obtained in this study suggest that poly(3HB-co-3HV) copolyesters, with >30 mol% 3HV, may be useful in biocompatible biomedical applications.