• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.28 no.2
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• pp.155-160
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• 2002

Oral squamous cell carcinoma (OSCC), the most common head and neck cancer, shows poor prognosis as a result of frequent local invasion and lymph node metastasis that is mediated by multiple proteolytic enzymes and angiogenesis. In recent reports, angiogenesis is known to play an important role in tumor invasion and metastasis. The purpose of this study was to determine the role of angiogenesis in OSCCs, particularly with respect to the invasive and the metastatic potential. The microvessel density (CD31) in 34 human OSCC cases were investigated by immunohistochemistry, and reviewed with respect to the invasiveness and the presence of lymph node metastasis and following results were obtained. The blood vessel density $(28.8{\pm}7.9)$ in the strong invasive cases were significantly higher than those $(23.3{\pm}6.9)$ in the weak invasive cases. (p<0.05) In the 14 cases with lymph node metastasis, the average blood vessel density was $28.5{\pm}9.6$. On the other hand, in the 20 cases without lymph node metastasis, the blood vessel density was $25.2{\pm}6.4$. The blood vessel density was not statistically related to lymph node metastasis. (p>0.05) These results suggest that angiogenesis may be related to the local invasion of OSCC and further research will be needed to investigate the possibility that antiangiogenic agent can be used as an anticancer agent for OSCC.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.3
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• pp.1363-1367
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• 2014

This research was conducted to compare differences in colon cancer lymphatic vessel invasion (LVI) with D2-40 antibody labeling and regular HE staining, blood vessel invasion (BVI) with CD34 antibody labeling and HE staining and to assess the possibility of using D2-40-LVI/CD34-BVI in combination for predicting stage II colon cancer prognosis and guiding adjuvant chemotherapy.Anti-D2-40 and anti-CD34 antibodies were applied to tissue samples of 220 cases of stage II colon cancer to label lymphatic vessels and small blood vessels, respectively. LVI and BVI were assessed and multivariate COX regression analysis was performed for associations with colon cancer prognosis. Regular HE staining proved unable to differentiate lymphatic vessels from blood vessels, while D2-40 selectively labeled lymphatic endothelial cell cytosol and CD34 was widely expressed in large and small blood vessels of tumors as well as normal tissues. Compared to regular HE staining, D2-40-labeling for LVI and CD34-labeling for BVI significantly increased positive rate (22.3% vs 10.0% for LVI, and 19.1% vs 9.1% for BVI). Multivariate analysis indicated that TNM stage, pathology tissue type, post-surgery adjuvant chemotherapy, D2-40-LVI, and CD34-BVI were independent factors affecting whole group colon cancer prognosis, while HE staining-BVI, HE staining-LVI were not significantly related. When CD34-BVI/D2-40-LVI were used in combination for detection, the risk of death for patients with two or one positive results was 5.003 times that in the LVI(-)&BVI(-) group (95% CI 2.365 - 9.679). D2-40 antibody LVI labeling and CD34 antibody BVI labeling have higher specificity and accuracy than regular HE staining and can be used as molecular biological indicators for prognosis prediction and guidance of adjuvant chemotherapy for stage II colon cancer.

• International Journal of Vascular Biomedical Engineering
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• v.3 no.2
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• pp.1-9
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• 2005

Flow-induced dilation of blood vessel is the result of a series of bioreaction in vascular endothelial cells(VEC). Shear stress change by blood flow in human artery or vein is sensed by the mechanoreceptor and responsible for such a chain reaction. The inositol(1,4,5)-triphophate($IP_3$) is produced in the first stage to elevate permeability of the intercellular membrane to calcium ions by which the cytosolic calcium concentration is consequently increased. This intracellular calcium transient triggers synthesis of EDRF and prostacyclin. The mathematical model of this VEC calcium dynamics is reproduced from the literature. We then use the Computational Fluid Dynamics(CFD) technique to investigate the blood stream dictating the VEC calcium dynamics. The pulsatile blood flow in a stenosed blood vessel is considered here as a part of study on thrombogenesis. We calculate the pulsating shear stress (thus its temporal change) distributed over the stenosed artery that is implemented to the VEC calcium dynamics model. It has been found that the pulsatile shear stress induces larger intracellular $Ca^{2+}$ transient plus much higher amount of EDRF and prostacyclin release in comparison with the steady shear stress case. It is concluded that pulsatility of the physiological shear stress is important to keep the vasodilation function in the stenosed part of the blood vessel.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.05a
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• pp.383-386
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• 2007

As the blood flow characteristics have been recognized to be closely related to various cardiovascular diseases, it is very important to predict them accurate enough in an efficient way. Thus, this paper proposes a one-dimensional spectral finite element model for the human blood vessels. The spectral finite element model is formulated in the frequency-domain by using the exact frequency dependent shape functions and applied to an ascending aorta.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.44 no.2 s.314
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• pp.10-17
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• 2007

The echo signal on ultrasonic transducer is a mixed signal from tissues, blood vessel walls, blood cells and noise. In this mixed-signal, the signal reflected from tissues and blood vessel walls is called clutter. It is necessary to extract pure blood signal from this mixed-signal, when measuring blood flow velocity with medical ultrasonic system The quality of measured blood flow velocity is highly dependent on sufficient attenuation of the clutter signals. In this paper, we suggest a clutter rejection method using ICA For simulation, the echo signals are generated by Field n ultrasonic simulation program In this echo signals, independent signals are separated by using ICA Then the blood signal is obtained from the separated signals. Blood flow velocity is measured by 2D autocorrelation method. We compare ICA clutter rejection method with PCA-based eigen filter method using both measured blood flow velocity profiles by 2D autocorrelation. In simulation results, ICA clutter rejection method can be better applied measuring blood flow velocity in noisy echo signals.

• Journal of the Institute of Convergence Signal Processing
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• v.15 no.4
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• pp.128-134
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• 2014

In this paper, we propose a detection method of the leukocyte motions in a microvessel by using spatiotemporal image analysis. The leukocyte motions that adhere to blood vessel walls can be visualized to move along the blood vessel wall's contours in a sequence of images. In this proposal method, we use the constraint that the leukocytes move along the blood vessel wall's contours and detect the leukocyte motions by using the spatiotemporal image analysis method. The generated spatiotemporal image is processed by a special-purpose orientation-selective filter and then subsequent grouping processes are done. The subsequent grouping processes select and group the leukocyte trace segments among all the segments obtained by simple thresholding and skeletonizing operations. Experimental results show that the proposed method can stably detect the leukocyte motions even when multiple leukocyte traces intersect each other.

• The Korean Journal of Physiology and Pharmacology
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• v.20 no.5
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• pp.533-538
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• 2016

Angiogenesis plays an essential role in embryo development, tissue repair, inflammatory diseases, and tumor growth. In the present study, we showed that endothelial nitric oxide synthase (eNOS) regulates retinal angiogenesis. Mice that lack eNOS showed growth retardation, and retinal vessel development was significantly delayed. In addition, the number of tip cells and filopodia length were significantly reduced in mice lacking eNOS. Retinal endothelial cell proliferation was significantly blocked in mice lacking eNOS, and EMG-2-induced endothelial cell sprouting was significantly reduced in aortic vessels isolated from eNOS-deficient mice. Finally, pericyte recruitment to endothelial cells and vascular smooth muscle cell coverage to blood vessels were attenuated in mice lacking eNOS. Taken together, we suggest that the endothelial cell function and blood vessel maturation are regulated by eNOS during retinal angiogenesis.

• Journal of International Society for Simulation Surgery
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• v.2 no.1
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• pp.17-25
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• 2015

Purpose In this paper, we propose a robust 3D vessel tracking algorithm by utilizing an active contour model and unscented Kalman filter which are the two representative algorithms on segmentation and tracking. Materials and Methods The proposed algorithm firstly accepts user input to produce an initial estimate of vessel boundary segmentation. On each Computed Tomography Angiography (CTA) slice, the active contour is applied to segment the vessel boundary. After that, the estimation process of the unscented Kalman filter is applied to track the vessel boundary of the current slice to estimate the inter-slice vessel position translation and shape deformation. Finally both active contour and unscented Kalman filter are inter-operated for vessel segmentation of the next slice. Results The arbitrarily shaped blood vessel boundary on each slice is segmented by using the active contour model, and the Kalman filter is employed to track the translation and shape deformation between CTA slices. The proposed algorithm is applied to the 3D visualization of chest CTA images using graphics hardware. Conclusion Through this algorithm, more opportunities, giving quick and brief diagnosis, could be provided for the radiologist before detailed diagnosis using 2D CTA slices, Also, for the surgeon, the algorithm could be used for surgical planning, simulation, navigation and rehearsal, and is expected to be applied to highly valuable applications for more accurate 3D vessel tracking and rendering.

• Parasites, Hosts and Diseases
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• v.30 no.4
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• pp.255-258
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• 1992

Two fully matured specimens were collected from the blood vessel of two fish, Tlreragra chatcogramma, which was bought at the Emun market of Seoul in May, 1985. The blood cuke has no pharynx and suckers. Its body surface was covered with minute spines forming fan-shaped groups, 15 Mm long. The intestine was H-shaped, and the ratio of esophageal length to body length was 1 : 6.1∼6.8. Irregular shaped testes were 109∼114 in number, occupying the intercecal space from cecal bifurcation to the genital pore. The present species was identified as Aporocotyle sheragrae. This is the first report of the species in Korea.

• The Journal of the Acoustical Society of Korea
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• v.29 no.2E
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• pp.73-85
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• 2010

In the diagnostic ultrasound (US) transducer technology, the high frequency US(HFUS) transducer over 20 MHz is one of the current issues to be pursued for better resolution with the expense of penetration. HFUS single element transducers and the mechanical scanning systems for imaging are reviewed, and HFUS array transducers are also briefly summarized. HFUS applications such as the human applications in ophthalmology and dermatology and small animal applications for research purposes are reviewed with vascular and blood imaging in this paper.