Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
권호 표지

THE CLINICOPATHOLOGICAL STUDY ON THE RELATION OF MICROVESSEL DENSITY AND AGGRESSIVENESS IN ORAL SQUAMOUS CELL CARCINOMA

구강편평세포암종의 미세혈관 밀도와 악성도간 상관관계에 관한 임상병리학적 연구

  • Lee, Gi-Cheol (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Myoung, Hoon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Lim, Sung-Sam (Department of Conservative Dentistry College of Dentistry, Seoul National University) ;
  • Kim, Myung-Jin (Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University)
  • 이기철 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 명훈 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 임성삼 (서울대학교 치과대학 치과보존학교실) ;
  • 김명진 (서울대학교 치과대학 구강악안면외과학교실)
  • Published : 2002.04.30
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Abstract

Oral squamous cell carcinoma (OSCC), the most common head and neck cancer, shows poor prognosis as a result of frequent local invasion and lymph node metastasis that is mediated by multiple proteolytic enzymes and angiogenesis. In recent reports, angiogenesis is known to play an important role in tumor invasion and metastasis. The purpose of this study was to determine the role of angiogenesis in OSCCs, particularly with respect to the invasive and the metastatic potential. The microvessel density (CD31) in 34 human OSCC cases were investigated by immunohistochemistry, and reviewed with respect to the invasiveness and the presence of lymph node metastasis and following results were obtained. The blood vessel density $(28.8{\pm}7.9)$ in the strong invasive cases were significantly higher than those $(23.3{\pm}6.9)$ in the weak invasive cases. (p<0.05) In the 14 cases with lymph node metastasis, the average blood vessel density was $28.5{\pm}9.6$. On the other hand, in the 20 cases without lymph node metastasis, the blood vessel density was $25.2{\pm}6.4$. The blood vessel density was not statistically related to lymph node metastasis. (p>0.05) These results suggest that angiogenesis may be related to the local invasion of OSCC and further research will be needed to investigate the possibility that antiangiogenic agent can be used as an anticancer agent for OSCC.

Keywords

References

  1. Spiro RH, Alfonso AE, Farr HW, Strong EW: Cervical node metastasis from epidermoid carcinoma of the oral cavity and oropharynx. A critical assessment of current staging. Am J Surg 128(4):562-567, 1974
  2. Weidner N, Folkman J, Pozza F, Bevilacqua P, Allred EN, Moore DH, Meli S, Gasparini G: Tumor angiogenesis: A new significant and independent prognostic indicator in early-stage breast carcinoma. J Natl Cancer Inst 84:1875-1887, 1992
  3. Fox SB: Tumor angiogenesis and prognosis. Histopathol 30:294-301, 1997
  4. Battegay: Angiogenesis: mechanic insights, neovascular diseases, and therapeutic prospects. J Mol Med 73(7):333-346, 1995
  5. Folkman J, Cotran RS : Relationship of vascular proliferation to tumor growth. Int Rev Exp Pathol 16 : 207-248, 1976
  6. Schiller JH, Bittner G : Potentiation of platinum antitumor effects in human lung tumor xenografts by the angiogenesis inhibitor squalamine : effects on tumor neovascularization. Clin Cancer Res 5 : 4287-4294, 1999
  7. Kenyon BM, Browne F, D’Amato RJ : Effects of thalidomide and related metabolites in a mouse corneal model of neovascularization. Exp Eye Res 64 : 971-978, 1997 https://doi.org/10.1006/exer.1997.0292
  8. Yamamoto E, Miyakawa A, Kohama G: Mode of invasion and lymph node metastasis in squamous cell carcinoma of the oral cavity. Head Neck Surg 6:938-947, 1984
  9. Sakamoto A, Oda Y, Iwamoto Y, Tsuneyoshi M. Expression of MT1-MMP, MMP-2, and TIMP-2 in human cartilagenous tumors with special emphasis on mesenchymal and dedifferentiated chondrosarcoma. J Cancer Res Clin Oncol 125: 541-548, 1999
  10. Ferriola PC, Cody V, Middleton EJ: Protein kinase C inhibition by plant flavonoids. Biochemical Pharmacology 38:1617-1624, 1989
  11. Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N, Shibuya M, Fukami Y: Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem 25;262(12):5592-5595, 1987
  12. Hill TD, Dean NM, Mordan LJ, Lau AF, Kanemitsu MY, Boynton AL: PDGF-induced activation of phospholipase C is not required for induction of DNA synthesis. Science 248:1660-1663, 1990
  13. Fox SB : Tumor angiogenesis and prognosis. Histopathol 30:294-301, 1997
  14. Dickinson AJ, Fox SB, Persad J : Quantification of angiogenesis as an independent predictor of prognosis in invasive bladder carcinomas. Br J Urol 74 : 762-766, 1994
  15. Jaeger TM, Weidner N, Chew K : Tumor angiogenesis correlates with lymph node metastasis in invasive bladder cancer. J Urol 154 : 69-71, 1995
  16. Takahashi Y, Kitadai Y, Bucana CD : Expression of vascular endothelial cell growth factor and its receptor, KDR, correlates with vascularity, metastasis, and proliferation of human colon cancer. Cancer Res 55 : 3964-3968, 1995
  17. Tanigawa N, Amaya H, Matsumura M : Extent of tumor vascularization correlates with the prognosis and hematogenous metastasis in gastric carcinomas. Cancer Res 56 : 2671-2676, 1996
  18. Gasparini G, Harris AL: Clinical importance of the determination of tumor angiogenesis in breast carcinoma: Much more than a new prognostic tool. J Clin Oncol 13:765-782, 1995
  19. Leedy DA, Trune DR, Kronz JD, Weidner N, Cohen JI: Tumor angiogenesis, the p53 antigen, and cervical metastasis in squamous cell carcinoma of the tongue. Otolaryngol Head Neck Surg 111:417-422, 1994
  20. Williams JK, Carlson GW, Cohen C, Derose PB, Hunter S, Jurkiewicz MJ: Tumor angiogenesis as a prognostic factor in oral cavity tumor. Am J Surg 168:373-380, 1994
  21. Pazouki S, Chisholm DM, Adi MM, Carmichael G, Farquharson M, Ogden GR, Schor SL, Schor AM: The association between tumour progression and vascularity in the oral mucosa. J Pathol 183:39-43, 1997
  22. Sion-Vardy N, Fliss DM, Prinsloo I, Shoham-Vardi I, Benharroch D: Neoangiogenesis in squamous cell carcinoma of the larynx - biological and prognostic associations. Pathol Res Pract 197(1):1-5, 2001
  23. Shpitzer T, Chaimoff M, Gal R, Stern Y, Feinmesser R, Segal K:Tumor angiogenesis as a prognostic factor in early oral tongue cancer. Arch Otolaryngol Head Neck Surg 122:865-868, 1996
  24. Gasparini G, Weidner N, Maluta S, Pozza F, Boracchi P, Mezzetti M, Testolin A, Bevilacqua P: Intratumoral microvessel density and p53 protein: correlation with metastasis in head and neck carcinoma. Int J Cancer 55:739-744,1993
  25. Folkman J, Shing Y : Angiogenesis. J Biol Chem 267 : 10931-10934, 1992
  26. Tokumo K, Kodama J, Seki N, Nakanishi Y, Miyagi Y, Kamimura S, Yoshinouchi M, Okuda H, Kudo T : Different angiogenic pathways in human cervical cancers. Gynecol Oncol 68 : 38-44, 1998
  27. Myoung H, Hong SD, Kim YY, Hong SP, Kim MJ: Evaluation of the anti-tumor and anti-angiogenic effect of paclitaxel and thalidomide on the xenotransplanted oral squamous cell carcinoma. Cancer Letters 163:191-200, 2001
  28. Gleich LL, Biddinger PW, Pavelic ZP, Gluckman JL : Tumor angiogenesis in T1 oral cavity squamous cell carcinoma: role in predicting tumor aggressiveness. Head Neck 18 : 343-346, 1996 https://doi.org/10.1002/(SICI)1097-0347(199607/08)18:4<343::AID-HED5>3.0.CO;2-Y
  29. Gleich LL, Zimmerman N, Wang YO, Gluckman JL : Angiogenic inhibition for the treatment of head and neck cancer. Anticancer Res 18 : 2607-2609, 1998