• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1746-1754
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• 2016

Recently, spherical beads entrapping quorum quenching (QQ) bacteria have been reported as effective moving QQ-media for biofouling control in MBRs for wastewater treatment owing to their combined effects of biological (i.e., quorum quenching) and physical washing. Taking into account both the mass transfer of signal molecules through the QQ-medium and collision efficiencies of the QQ-medium against the filtration membranes in a bioreactor, a cylindrical medium (QQ-cylinder) was developed as a new shape of moving QQ-medium. The QQ-cylinders were compared with previous QQ-beads in terms of the QQ activity and the physical washing effect under identical loading volumes of each medium in batch tests. It was found that the QQ activity of a QQ-medium was highly dependent on its specific surface area, regardless of the shape of the medium. In contrast, the physical washing effect of a QQ-medium was greatly affected by its geometric structure. The enhanced anti-biofouling property of the QQ-cylinders relative to QQ-beads was confirmed in a continuous laboratory-scale MBR with a flat-sheet membrane module.

• BMB Reports
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• 제33권6호
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• pp.483-492
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• 2000

The Herpes simplex virus type 1 (HSV-1) strain F glycoprotein H (gH) gene in the pHLB-4 plasmid was recombinated into a baculovirus expression vector (lacZ-HcNPV) to construct a recombinant virus GH-HcNPV expressing gH. The sequences of gH and its expression were analyzed. The gH gene was located in the 6.41 kb BglII fragment. The open reading frame (ORF) of the gH gene was 2,517 bp and codes 838 amino acid residues. Insect cells infected with this recombinant virus synthesized a high level of the matured and gX-gH fusion protein with approximately 112 kDa. The fusion gH protein was localized on the membrane of the insect cells as seen by using immunofluorescence assay and accumulated in the cultured media by the SDS-PAGE and immunoprecipitation assays. The amino acid sequence presents additional characteristics compatible with the structure of a viral glycoprotein: signal peptide, putative glycosylation sites and a long C-terminal transmembrane sequence. Antibodies raised in mice to this recombinant protein recognized viral gH and neutralized the infectivity of HSV-1 in vitro. These results demonstrate that it is possible to produce a mature protein by gene transfer in eukaryotic cells, and indicate the utility of the HcNPV-insect cell system for producing and characterizing eukaryotic proteins. Furthermore, the neutralizing antibodies would appear to protect mice against HSV; accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

• BMB Reports
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• 제53권12호
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• pp.628-633
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• 2020

WNT11 is a member of the non-canonical Wnt family and plays a crucial role in tumor progression. However, the regulatory mechanisms underlying WNT11 expression are unclear. Tumor necrosis factor-alpha (TNFα) is a major inflammatory cytokine produced in the tumor microenvironment and contributes to processes associated with tumor progression, such as tumor invasion and metastasis. By using site-directed mutagenesis and introducing a serial deletion in the 5'-regulatory region of WNT11, we observed that TNFα activates the early growth response 1 (EGR1)-binding sequence (EBS) in the proximal region of WNT11 and that the transcription factor EGR1 is necessary for the TNFα-induced transcription of WNT11. EGR1 bound directly to the EBSs within the proximal 5'-regulatory region of WNT11 and ectopic expression of EGR1 stimulated WNT11 promoter activity, whereas the knockdown of EGR1 expression by RNA interference reduced TNFα-induced WNT11 expression in T47D breast cancer cells. We also observed that mitogen-activated protein kinases (MAPK), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 kinase mediated TNFα-induced transcription of WNT11 via EGR1. Our results suggest that EGR1 directly targets WNT11 in response to TNFα stimulation in breast cancer cells.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.66.2-66
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• 2003

A gain-of-function mutant, SHM-11 obtained through gamma-ray mutagenesis, is resistant to rice blast caused by Magnaporthe grisea while wild type Sanghaehyanghyella is highly susceptible to the same disease. The resistance in the mutant was not race-specific when we tested with four races (KJ-201, KI-1113a, KI-313, KI-409) of M. grisea. To identify genes involved disease resistance in the gain-of-function mutant, genes specifically expressed in the mutant were selected by suppression subtractive hybridization using cDNAS of blast-inoculated mutant and wild type as a tester and a driver, respectively, Random 200 clones from the subtracted library were selected and analyzed by DNA sequencing. The sequenced genes represented three major groups related with disease resistance； genes encoding PR proteins, genes probably for phytoalexin biosynthesis, and genes involved in disease resistance signal transduction. A gene encoding a putative receptor-like protein kinase was identified as highly expressed only in the gain-of-function mutant after blast infection. The role of the putative receptor-like protein kinase gene during blast resistance will be further studied.

• 융합신호처리학회논문지
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• 제16권2호
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• pp.56-62
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• 2015

본 연구에서는 현대인들의 일상생활 중 대부분의 시간을 보내는 좌식생활 중에 무구속적으로 장시간 심장활동상태를 모니터링하기 위한 의자형 무구속 심전도 모니터링 시스템을 설계 및 구현하였다. 구현된 시스템은 옷을 입은 상태에서도 측정이 가능하며, 동잡음에도 비교적 강한 장점을 가진다. 이를 위하여 의자의 등받이 부분에 무구속 전극을 배치하였으며, 미세한 생체신호를 증폭하고, 측정 시 발생 할 수 있는 동잡음 및 기저선의 변화, 상용전원에 의한 전원잡음을 제거하기 위하여 증폭기 및 필터를 설계하였다. 또한 제어부와 무선전송부를 구현하여 아날로그 신호를 디지털 신호로 변환하고 생체신호를 PC와 스마트폰으로 전송함으로써 일상생활 중 지속적인 심장활동 상태의 모니터링이 가능하다. 구현된 시스템의 성능을 평가하기 위하여 Ag/AgCl 전극을 사용한 시스템과 비교 실험을 수행하였으며, 실험 결과 구현된 시스템의 무구속적으로 심장활동 상태 모니터링이 가능함을 확인하였다.

• 대한의용생체공학회:의공학회지
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• 제35권6호
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• pp.234-241
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• 2014

This study was designed to investigate the effects of stimulation intensity and inter-electrode distance on the parameters of the measured sensory nerve signal. 30 healthy subjects participated in this study. Sensory nerve signals were elicited by four different pulse amplitudes, i.e., 3, 6, 9, 12 mA, with the pulse width fixed at $500{\mu}s$. The sensory nerve signals elicited by the four different pulse amplitudes were measured by four different inter-electrode distances (20, 30, 40, and 50 mm). We extracted four parameters (pulse amplitude, pulse width, pulse area, and latency time from stimulation) from the sensory nerve signals. The measured pulse amplitude and pulse width were increased when the measuring inter-electrode distance was increased while the stimulating pulse amplitude was fixed. The measured pulse amplitude was saturated with the stimulating pulse amplitudes of over 6 mA while measuring inter-electrode distance. Under the same condition, measured pulse width was increased, and sensory nerve signal was initiated early. Sensory nerve signals, specially those of pulse amplitude, were distorted by a differential amplification method that commonly measures the human body signal. The experimental results indicate that the differential amplification method is required to be replaced when measuring nerve signals. Our observations suggested that the hyperpolarization of the action potential of the sensory nerve signal for preventing distortion could be used to clarify the correlation between the parameters of the sensory nerve signals and quantification of sensations.

• 전기전자학회논문지
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• 제11권1호통권20호
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• pp.24-29
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• 2007

본 연구에서 심리적으로 안정된 상태에서 맥율을 측정할 수 있는 장치를 개발하였다. 개발된 시스템은 뇌파, 호흡파, 맥동파를 검출하는 하드웨어장치와 이들 신호를 획득하고 처리하는 소프트웨어로 구성하였다. 뇌파는 전두부에서 쌍극형으로 유도하였고, 호흡은 서미스터 브리지를 이용하여 구성된 변환기를 사용하여 비강 전부에서 유도하였으며 맥동파는 귀볼에서 유도한 용적맥파를 사용하였다. 피검자의 심리적 안정된 상태의 판정은 뇌파의 스펙트럼을 이용하였다. 맥율의 결정은 원전에 따라 1호흡 당 맥동수를 사용하였다. 개발된 장치를 사용하여 맥율검출 실험을 한 결과, 뇌파의 주파수 대역별 구분, 안정된 호흡신호의 검출과 이득 조절이 되는 용적맥파의 검출이 실시간으로 가능하였다. 그리고 검출된 신호로부터 맥율을 검출할 수 있었다.

• 한국지능시스템학회논문지
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• 제24권5호
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• pp.457-461
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• 2014

생물학적 신경 세포의 모델링을 위한 펄스타입 실리콘 뉴런 회로를 $0.18{\mu}m$ CMOS 공정을 이용하여 반도체 집적회로로 설계하였다. 제안하는 뉴런 회로는 입력 전류신호를 위한 커패시터 입력단과, 출력 전압신호 생성을 위한 증폭단 및 펄스신호 초기화를 위한 MOS 스위치로 구성된다. 전압신호 입력을 전류신호 출력으로 변환하는 기능의 시냅스 회로는 몇 개의 PMOS와 NMOS 트랜지스터로 이루어지는 범프회로를 사용한다. 제안하는 뉴런 모델의 검증을 위하여, 2개의 뉴런과 시냅스가 직렬연결된 뉴런체인을 구성하여 SPICE 모의실험을 실시하였다. 모의실험 결과, 뉴런신호의 생성과 시냅스 전달특성의 정상적인 동작을 확인하였다.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1996년도 추계학술대회
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• pp.277-280
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• 1996

We have developed a ground-isolation circuit in order to reduce the noise of the internal controller system for the total artificial heart(TAH) and ventricular assist device(VAD). Using the ground-isolation technique, we could transmit the analog target signal to other pheriperal device including IBM PC via RS232C and polygraph, with no noise. Experimental results of VAD showed that there was less impulsive noise in current signal which caused in our previous conventional system. Therefore it could be proved that implementation of isolation technique is very effective to improve the signal to noise ratios of analog signal transmission for TAH or VAD.

• 대한의용생체공학회:의공학회지
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• 제29권3호
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• pp.198-204
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• 2008

Uroflowmetry is a non-invasive clinical test useful for screening benign prostatic hyperplasia(BPH) common in the aged men. The current standard way to obtain the urinary flow rate is to continuously acquire the urine weight signal proportional to volume over time. The present study proposed an alternative technique measuring pressure to overcome noise problems present in the standard weight measuring technique. Experiments were performed to simultaneously acquire both weight and pressure changes during urination of 9 normal men. Noise components were separated from volume signals converted from both weight and pressure signals based on the polynomial signal model. Signal-to-noise ratio was defined as the ratio of the energies between signal and noise components of the measured volume changes, which was 8.5 times larger in the pressure measuring technique, implying that cleaner signal could be obtained, more immune to noisy environments. When four important diagnostic parameters were estimated, excellent correlation coefficients higher than 0.99 were resulted with mean relative errors less than 5%. Therefore, the present pressure measurement seemed valid as an alternative technique for uroflowmetry.