• Proceedings of the Korean Information Science Society Conference
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• 2012.06b
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• pp.453-455
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• 2012

생물 검정(Bioassay)이란 생체 조직이나 분자의 구조 분석이나 기능 해석, 화합물이나 약에 의한 영향성을 실험하기 위해 실험체 조직과 약물의 상호작용에 의한 생성물의 양적 세기를 측정하는 과학적 실험 방법의 총칭이다. 바이오 어세이 실험 방법은 Gas Chromatography, 시험관 전기영동(Capillary Electrophoresis), 핵자기공명(NMR) 등의 다양한 실험 데이터를 포함한다. 결과로 생성된 실험 데이터를 정량적으로 분석하기 위해서는 일관성을 위해 얻어진 데이터를 정렬하는(alignment) 과정을 거쳐야 한다. 본 연구에서는 알려진 정렬 알고리즘들을 비교하기 위해, 알고리즘의 유형별로 분류하고 그 결과물을 분석하여 성능을 비교함과 동시에 특성을 파악하고자 한다.

• Environmental Analysis Health and Toxicology
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• v.19 no.1
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• pp.25-32
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• 2004

Aim of the present study was the development of a bioassay which enables the detection of toxic effects of heavy metal ions to a bacterium, Escherichia coli. Inhibition effects of the metals on growth rates of the bacterium were studied in the absence or presence of fulvic acid. This method does not clearly differentiate among metals, but does detect overall AGB inhibition rate (toxicity) for 5 different heavy metals. The toxicity of the metals in the absence of fulvic acid in the same testing conditions was significantly increased in following order: Hg < Pb, Zn < Cd < Cu, whereas the inhibition rate (toxicity) in the presence of FA was shown to be increased In following order: Cd < Pb, Hg < Cu < Zn. The results of the present study indicate that this simple and fast biomonitoring assay with direct exposure of E coli. might be a valuable supplement to analytical methods of contaminated media.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2003.10a
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• pp.166-166
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• 2003

• Archives of Pharmacal Research
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• v.25 no.6
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• pp.903-909
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• 2002

Research in the cytokine field has grown exponentially in recent years, and the validity of such studies relies heavily on the appropriate measurement of levels of cytokines in various biological samples. Transforming growth factor (TGF)-$\beta$, a hormonally active polypeptide found in normal and transformed tissue, is a potent regulator of cell growth and differentiation. The most widely used bioassay for TGF-$\beta$ is the inhibition of the proliferation of mink lung epithelial cells. Though detection of [$^3$H]thymidine incorporation is more sensitive than the MTT assay, it presents some disadvantages due to the safety and disposal problems associated with radioisotopes. In this study, we attempted to ascertain the experimental conditions which could be used for measuring the in vitro biological activity of TGF-$\beta$ in a safer and more sensitive way compared with the currently available methods. We compared the commonly used method, the MTT assay, to the XTT assay using different parameters including cell number, incubation time and the wave length used for detecting the product. We examined the anti-proliferative activities of TGF-$\beta$ in three different cell lines: Mv-1-Lu mink lung epithelial cells, MCF10A human breast epithelial cells and H-ras-transformed MCF10A cells. Herein, we present an experimental protocol which provides the most sensitive method of quantifying the biological activity of TGF-$\beta$, with a detection limit of as low as 10 pg/ml: Mv-1-Lu or H-ras MCF10A cells ($1{\times}10^5/well$) were incubated with TGF-$\beta$ at $37^{\circ}C$ in a humidified $CO_2$ incubator for 24 hr followed by XTT treatment and determination of absorbance at 450 or 490 nm. Our results may contribute to the establishment of an in vitro bioassay system, which could be used for the satisfactory quantitation of TGF-$\beta$.

• Korean journal of applied entomology
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• v.32 no.1
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• pp.24-29
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• 1993

Experiments were conducted to study effects of different bioassay methods on toxicity to the German cockroach (Blattella germanica L.) ith six insecticides. For male adults, by dry film method, the {TEX}$LC_{50}${/TEX} value of chlorpyrifos. fenvalerate, propoxur, permethin, DDVP, and hydramethylnon was 1.79, 1.87, 3.04, 4.37, 32.72, and 270.81 ppm, respectively and the same tendency was shown in the female. For male adults, by topical micro-application method, the {TEX}$LD_{50}${/TEX} value of DDVP and fenvalerate, chlorpyrifls, permethrin, propoxur, hydramethylnon was 2.63, 3.79, 4.51, 6.73, 44.99 $\mu\textrm{g}$/g, respectively and the same tendency was shown in the female. From the diet toxicity method against male adults, the CT value (Concentration%$\times${TEX}$LT_{50}${/TEX}) showed that chlorpyrifos was rapid effective and hydramethylnon was residual effective.

• CELLMED
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• v.9 no.1
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• pp.1.1-1.13
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• 2019

Background: Contents of bioactive substances extractable from different parts of terrestrial plants vary enormously. Aim: To ascertain that parts of Withania somnifera other than its roots can also be used for prevention and cure of unavoidable stress triggered central hypersensitivity to pain. Material and Methods: Groups of male or female mice treated either with Withania somnifera extracts or with metformin, aspirin, imipramine, diazepam and niacin for 11 consecutive days were subjected to "foot-shock stress-induced hyperthermia" and "hot plate" tests on the 1st, 5th, 7th, and 10th days of the experiments. On the 11th day, they were subjected to tail suspension test and on 12th day pentobarbital hypnosis test. Results: Except for diazepam and imipramine, protective effects of all other tested drugs as well as of the Withania somnifera extracts against stress-induced central hypersensitivity to pain were accompanied by their preventive effects against foot-shock stress-induced body weight losses. All observed stress response suppressing effects of all test agents increased with increasing numbers of treatment days. However, mean duration of pentobarbital-induced sleep was shorter in the extracts treated groups and longer in the diazepam treated ones only. Conclusions: Reported observations reveal that pharmacological activity profile of Withania somnifera extracts in male and female mice are almost identical, and are not like those of several drugs currently often prescribed for the treatment of diabetes-associated comorbidities. Withanolides are not the only extractable bioactive constituents of Withania somnifera. The described bioassay system is well suited for pharmacological standardization of diverse types of Withania somnifera extracts.

• Nuclear Engineering and Technology
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• v.56 no.6
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• pp.2050-2056
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• 2024

Handling of radioisotopes may cause external and internal contamination to occupational workers while using radiation for medical purposes. This research aims to monitor the internal hazard of occupational workers who handle ¹³¹I. Two methods are used: in vivo or direct method and in vitro or indirect method. The in vivo or direct method was performed by assessing thyroid intake with a thyroid uptake monitoring machine. The in vitro or indirect method was performed by assessing urine samples with the help of a gamma-ray spectroscopy practice using a High-Purity Germanium (HPGe) Detector. In this study, fifty-nine thyroid counts and fifty-nine urine samples were collected from seven occupational workers who were in charge of ¹³¹I at the National Institute of Nuclear Medicine and Allied Sciences (NINMAS), Dhaka. The result showed that the average annual effective dose of seven workforces from thyroid counts were 0.0208 mSv/y, 0.0180 mSv/y, 0.0135 mSv/y, 0.0169 m Sv/y, 0.0072 mSv/y, 0.0181 mSv/y, 0.0164 mSv/y and in urine samples 0.0832 mSv/y, 0.0770 mSv/y, 0.0732 mSv/y, 0.0693 mSv/y, 0.0715 mSv/y, 0.0662 mSv/y, 0.0708 mSv/y.The total annual effective dose (in vivo and in vitro method) was found among seven workers in average 0.1039 mSv/y, 0.0950 mSv/y, 0.0868 mSv/y, 0.0862 mSv/y, 0.0787 mSv/y, 0.0843 mSv/y, 0.0872 mSv/y. Following the rules of the International Commission on Radiological Protection (ICRP), the annual limit of effective dose for occupational exposure is 20 mSv per year and the finding values from this research work are lesser than this safety boundary.

• Korean Journal of Poultry Science
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• v.10 no.1
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• pp.35-45
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• 1983

This experiment was performed to compare the techniques of the metabolizable energy determination and to, examine the problems associated with metabolizable energy determination of poultry feedingstuffs. The feedingstuffs evaluated herein, were corn, sorghum, barley. fish meal and soybean meal The results obtained were as followings : 1. When comparing AME and AMEn values of five feedingstuffs(measured) by Total Collection ana Index Methods there was no significant difference (P＞.05) between two methods except fish meal. 2. The AME$_{F}$ values of feedingstuffs were not different (P＞.05) from AME values. which implied that bioassay of AME$_{F}$ be applicable to rapid measure AME value of feedingstuffs for poultry and the problems connecting to AME$_{F}$ determination were discussed. 3. The AME$_{FC}$ and TME values of corn, sorghum and barley were significantly higher (P＜.05) than AME and AME$_{F}$ values but those of fish meal and soybean meal were similar or lower than AME and AME$_{F}$ values. This fact was indicative that the techniques measuring AME$_{FC}$ and TME has some depressing factors when fish meal and soybean meal were determined. 4. Comparing of TME, AME$_{FC}$ and T-AME values tile TME values of corn sorghum and barley were significantly higher(P＜.05) than AME$_{FC}$ and T- AME values. But that of fish meal and soybean meal was much lower(P＜ .05)comparing with AME$_{FC}$ and T-AME values. According]y, it night be concluded that the relationship among the values of TME. AME$_{FC}$ and T-AME was shown great variation according to the feedingstuffs, especially in the protein diets.

• Biomolecules & Therapeutics
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• v.15 no.1
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• pp.46-51
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• 2007

Bioassay-directed chromatographic fractionation of an ethyl acetate extract from leaves of sweet potato (Ipomoea batatas L.) afforded six quinic acid derivatives: 3,5-epi-dicaffeoylquinic acid (1), 3,5-dicaffeoylquinic acid (2), methyl 3,5-O-dicaffeoylquinate (3), methyl 3,4-dicaffeoylquinate (4), methyl 4,5-dicaffeoylquinic acid (5),4,5-dicaffeoylquinate (6), and two phenolic compounds: caffeic acid (7) and caffeic acid methyl ester (8) together with three flavonoids: quercetin 3-O-${\beta}$-D-glucopyranoside (9), quercetin 3-O-${\beta}$-D-glucopyranoside, isoquercitrin (10) and kaempferol 3-O-${\beta}$-D-glucopyranoside (11). The structures of these compounds were elucidated by the aid of spectroscopic methods. These compounds were assessed for antioxidant activities using three different cell-free bioassay systems. All isolates except 11 showed potent DPPH and superoxide anion radicals scavenging, and lipid peroxidation inhibitory activities. 3,5-epi-DCQA (1) and methyl quinates (3-5) along with flavonoide 9 were isolated for the first time from this plant.

• Proceedings of the Korean Society of Crop Science Conference
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• 1989.02a
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• pp.58-59
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• 1989