Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Verification of AGB (alpha-glucosidase biosynthesis) Bacterial Bioassay of Metal Toxicity and Inhibition Effect of Fulvic Acid (EA)

박테리아(E. Coli)의 AGB (alpha-glucosidase biosynthesis)를 이용한 중금속의 독성시험과 풀빅산에 의한 독성 억제효과

  • 김재현 (동덕여자대학교 자연과학대학 보건관리학과) ;
  • 김명길 (경기도 보건환경연구원) ;
  • 엄애선 (한양대학교 생활과학대학 식품영양학과)
  • Published : 2004.03.01
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Abstract

Aim of the present study was the development of a bioassay which enables the detection of toxic effects of heavy metal ions to a bacterium, Escherichia coli. Inhibition effects of the metals on growth rates of the bacterium were studied in the absence or presence of fulvic acid. This method does not clearly differentiate among metals, but does detect overall AGB inhibition rate (toxicity) for 5 different heavy metals. The toxicity of the metals in the absence of fulvic acid in the same testing conditions was significantly increased in following order: Hg < Pb, Zn < Cd < Cu, whereas the inhibition rate (toxicity) in the presence of FA was shown to be increased In following order: Cd < Pb, Hg < Cu < Zn. The results of the present study indicate that this simple and fast biomonitoring assay with direct exposure of E coli. might be a valuable supplement to analytical methods of contaminated media.

Keywords

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