• Journal of Plant Biotechnology
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• 제38권1호
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• pp.15-21
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• 2011

Agrobacterium tumefaciens와 운반체 416을 이용하여 cryIAc1 유전자가 형질전환된 배추 14개체를 선발하였다. Southern blot을 통하여 분석한 결과 1 사본 유전자가 도입된 개체가 6개이었으며 backbone 염기서열이 포함되지 않은 경우는 4개체이었다. LB 인접서열 분석 결과, 416-2과 416-3은 23 bp의 LB 부위가 남아있는 동일한 염기서열을 보였고, 416-9 경우 15 bp가 남았으며, 416-17 경우 LB를 포함하여 안쪽의 염기서열의 최대 36 bp의 결실이 확인되었다. T-DNA의 염기서열을 제외한 배추 gDNA의 499 bp의 LB 인접염기서열은 B. oleracea의 염기서열과 96% 상동성을 가진 유전자로 확인되었다. RB border 인접서열 분석용 primer를 제작하여 PCR을 수행한 결과 모두 834 bp의 염기서열을 확인하였고, vector의 구성 요소 중 cryIAc1의 3' 말단 부위, nos-terminator 부위와 52 bp 및 배추 gDNA RB 인접염기서열로 확인되었다. RB 염기서열은 확인할 수 없었으며 nos-terminator 3' 말단의 21개의 염기를 포함하여 모두 58개의 염기서열이 결실된 것을 확인하였다. 형질전환식물체를 제작할 경우 발생하는 전이유전자나 식물의 염색체에 염기 결실은 매우 다양한 형태로 나타난다. 이번 실험의 경우, 전이유전자가 삽입된 위치에서 약 10 bp의 배추의 gDNA 염기가 결실된 것이나 삽입된 전이유전자의 RB 말단부분이 결실된 것은 예상할 수 있는 결과였지만, 특히 전이유전자의 말단인 nos-terminator 3' 끝에 65 bp 정도의 배추의 다른 유전자가 삽입되어 있다는 것을 확인하였고 이는 기대하지 않았던 결과였다. 삽입된 다른 배추유전자의 절편은 앞으로 더 많은 연구를 통하여 위치와 기능확인이 필요할 것이다.

• The Plant Pathology Journal
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• 제30권4호
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• pp.384-396
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• 2014

Vegetative compatibility groups (VCGs) are determined for many fungi to test for the ability of fungal isolates to undergo heterokaryon formation. In several fungal plant pathogens, isolates belonging to a VCG have been shown to share significantly higher genetic similarity than those of different VCGs. In this study we sought to examine the relationship between VCG and genetic similarity of an important cool season turfgrass pathogen, Sclerotinia homoeocarpa. Twenty-two S. homoeocarpa isolates from the Midwest and Eastern US, which were previously characterized in several studies, were all evaluated for VCG using an improved nit mutant assay. These isolates were also genotyped using 19 microsatellites developed from partial genome sequence of S. homoeocarpa. Additionally, partial sequences of mitochondrial genes cytochrome oxidase II and mitochondrial small subunit (mtSSU) rRNA, and the atp6-rns intergenic spacer, were generated for isolates from each nit mutant VCG to determine if mitochondrial haplotypes differed among VCGs. Of the 22 isolates screened, 15 were amenable to the nit mutant VCG assay and were grouped into six VCGs. The 19 microsatellites gave 57 alleles for this set. Unweighted pair group methods with arithmetic mean (UPGMA) tree of binary microsatellite data were used to produce a dendrogram of the isolate genotypes based on microsatellite alleles, which showed high genetic similarity of nit mutant VCGs. Analysis of molecular variance of microsatellite data demonstrates that the current nit mutant VCGs explain the microsatellite genotypic variation among isolates better than the previous nit mutant VCGs or the conventionally determined VCGs. Mitochondrial sequences were identical among all isolates, suggesting that this marker type may not be informative for US populations of S. homoeocarpa.

• 대한전자공학회논문지SD
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• 제44권2호
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• pp.127-138
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• 2007

본 논문에서는 최대길이 의사무작위 이진 시퀀스(m-시퀀스)의 쉬프트-덧셈 특성에 근거한 위상천이를 이용하여 회로 출력에 나타나는 X-값을 효과적으로 마스크 함으로써 내장된 자체 테스트를 실현할 수 있는 기법을 제안한다. 이 기법은 패턴생성기인 LFSR의 출력을 적절하게 위상천이 하여 마스크 패턴을 생성할 수 있는 위상천이 네트워크를 이용한다. 테스트 절차 동안에 각 스캔 체인에 인가되는 마스크 패턴의 위상 천이 수는 재구성 가능하다. LFSR의 출력을 적절하게 위상 천이하여 모든 스캔 체인 마스크 패턴을 생성할 수 있는 위상천이 네트워크 합성 알고리즘을 제안한다. 본 논문에서 제안하는 X-마스크 회로는 각 스캔 체인 마스크 패턴을 생성할 수 있는 후보 위상천이 수가 많기 때문에 하드웨어 오버헤드를 효과적으로 감축할 수 있다. 실험을 통하여 제안된 위상천이를 이용한 X-마스크 회로는 기존의 연구 결과보다 훨씬 적은 저장공간과 하드웨어 오버헤드를 필요로 함을 증명한다.

• Journal of information and communication convergence engineering
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• 제12권3호
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• pp.168-172
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• 2014

In this paper, we propose an algorithm that can efficiently detect and monitor multiple ships in real-time. The proposed algorithm uses morphological operations and edge information for detecting and tracking ships. We used smoothing filter with a $3{\times}3$ Gaussian window and luminance component instead of RGB components in the captured image. Additionally, we applied Sobel operator for edge detection and a threshold for binary images. Finally, object labeling with connectivity and morphological operation with open and erosion were used for ship detection. Compared with conventional methods, the proposed method is meant to be used mainly in coastal surveillance systems and monitoring systems of harbors. A system based on this method was tested for both stationary and non-stationary backgrounds, and the results of the detection and tracking rates were more than 97% on average. Thousands of image frames and 20 different video sequences in both online and offline modes were tested, and an overall detection rate of 97.6% was achieved.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 학술대회 논문집 정보 및 제어부문
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• pp.261-263
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• 2005

In this paper, we implement an identification system using the vein image of the hand. The vein pattern is obtained in the grey-scale 2D image through the infrared-red imaging from back of the hand. Since the frame has lack of clearance, we use some enhancing methods such as the complement, addition, and multiplication to the image to increase the contrast. After Wiener filtering for smoothness of the vein pattern, we transform the image into the binary image with mean function. The binarized image is session thinned and the cross-points in the vein tree are obtained by calculating the number of pixels connected because the image is shaped as a tree. We choose the point and find the nearest to the center if it has majority, where we find the two end points of the selected line. We can get the angle between the two lines joined at the cross-point and store its coordinates, angle, and label the values. The values are used as the feature vector of the vein pattern. This procedure is similar to the human cognition sequences. It is shown that the proposed method is simple for the vein recognition.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제8권7호
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• pp.2449-2463
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• 2014

Hierarchical B-frame coding was introduced into H.264/SVC to provide temporal scalability and improve coding performance. A content analysis-based adaptive group of picture structure (AGS) can further improve the coding efficiency, but damages the inter-frame correlation and temporal scalability of hierarchical B-frame to different degrees. In this paper, we propose a group of pictures (GOP) adaptation coding method based on the positions of video cuts. First, the cut positions are accurately detected by the combination of motion coherence (MC) and mutual information (MI); then the GOP is adaptively and proportionately set by the analysis of MC in one scene. In addition, we propose a binary tree algorithm to achieve the temporal scalability of any size of GOP. The results for test sequences and real videos show that the proposed method reduces the bit rate by up to about 15%, achieves a performance gain of about 0.28-1.67 dB over a fixed GOP, and has the advantages of better transmission resilience and video summaries.

• 전자공학회논문지S
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• 제36S권6호
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• pp.9-16
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• 1999

이 논문에서는 렘펠의 D-준동현사상을 바탕으로 하여 드브루인 수열을 만드는 효과적인 알고리즘을 제안한다. 이 알고리즘에서는 k차 드브루인 수열에서 n차 드브루인 수열의 다음 비트을 만드는데 필요한 배타논리합 연산수는, r의 2진 표현에서 1의 갯수를 W(r)이라 쓸 때, $k(2^{W(n-k)}-1)$쯤임을 보인다: 딸서, 드브루인 함수를 잘 고르면 이 수는 k가 된다.

• 한국식물병리학회지
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• 제11권1호
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• pp.66-72
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• 1995

The coat protein (CP) gene of cucumber mosaic virus-As (CMV-As) strain was engineered for expression in the plant by using the cauliflower mosaic virus 35S transcript regulatory sequences. The CP gene was cloned into an Agrobacterium-derived binary vector. A chimeric gene was constructed by the cDNA of CMV-As CP and plant expression vector pBI121. The clone, pCMAS66, was first introduced into the phagemid vector pSPORT1 for situating sense orientation for translation and making restriction sites in order to re-introduce plant expression vector, pHI121. The resulting subclone pCASCP02 and plant expression vector pBI121 were treated with BamHI-SacI for excising the target gene and removing GUS gene, respectively. After Agrobacterium transformation by freeze-thaw technique, the clone, pCMASCP121-123 which contains sense orientation of the target gene, was selected and confirmed by restriction endonuclease analysis. The CMV-As CP gene was introduced into A. tumefaciens. The results on tobacco plant transformation with the vector system revealed that the system could be successfully introduced and showed high frequency of selection to putative transformations.

• 원예과학기술지
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• 제35권3호
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• pp.323-332
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• 2017

Homology-specific transcriptional and post-transcriptional silencing, an intrinsic mechanism of gene regulation in most eukaryotes, can be induced by anti-sense, co-suppression, or hairpin-based double-stranded RNA. Hairpin-based RNA interference (RNAi) has been applied to analyze gene function and genetically modify crops. However, RNAi vector construction usually requires high-cost cloning steps and large amounts of time, or involves methods that are protected by intellectual property rights. We describe a more effective method for generating intron-spliced RNAi constructs. To produce intron-spliced hairpin RNA, an RNAi cassette was ligated with the first intron and splicing sequences of the Brassica rapa ssp. pekinensis histone deacetylase 1 gene. This method requires a single ligation of the PCR-amplified target gene to SpeI-NcoI and SacI-BglII enzyme sites to create a gene-specific silencing construct. We named the resulting binary vector system pKHi and verified its functionality by constructing a vector to silence DIHYDROFLAVONOL 4-REDUCTASE (DFR), transforming it into tobacco plants, and confirming DFR gene-silencing via PCR, RT-qPCR, and analysis of the accumulation of small interfering RNAs. Reduction of anthocyanin biosynthesis was also confirmed by analyzing flower color of the transgenic tobacco plants. This study demonstrates that small interfering RNAs generated through the pKHi vector system can efficiently silence target genes and could be used in developing genetically modified crops.

• 센서학회지
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• 제11권1호
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• pp.28-38
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• 2002

비디오 센서에 의한 감시 및 관측(VSAM)에 있어 사람은 중요하면서도 해석하기 어려운 목표이다. 본 논문에서는 연속 영상에서 자동으로 사람을 추적하거나 행위를 해석하는 작업에 선행하는 절차로서, 그 수를 계측하는 방법에 대해 다룬다. 정적인 비디오 카메라의 영상에서 사람만을 추출해낸 이진 영상을 얻고, 최상위 화소만을 연결한 선에서 지역최소점을 근거로 사람군을 나눈다. 이와 같이 나누어진 각 영역의 특성은 군내에서의 사람들의 상대적 위치에 따라 변화하게 되며, 이를 구분하기 위하여 완전 가림, 부분적 가림, 완전 분리된 개인, 오분리의 4가지 상태를 정의하였다. 연속적인 영상에서 나누어진 영역들의 상태가 변화하여가는 과정을 분석하여 관측 지역내 사람의 수를 추정하였으며, 이의 유용성을 실제 실험을 통하여 확인하였다.