• Korean Journal of Optics and Photonics
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• v.13 no.4
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• pp.295-300
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• 2002

We developed an optical transmitter and receiver for an electrical time division multiplexed (ETDM) 20 Gb/s optical transmission system, and experimentally investigated their characteristics. Especially, the clock extraction circuit, which is a key component in realizing broadband optical transmission receivers, was realized by using an NRZ-to-PRZ converter implemented with a half-period delay line and an EX-OR, a high-Q bandpass filter using a cylindrical dielectric resonator, and a microstrip coupled-line bandpass filter. Finally, the bit-error-rate of demultiplexed 10 Gb/s electrical signal after back to-back transmission was measured, and a high receiver sensitivity [-26.2 dBm for NRZ ($2^{7}-1$) pseudorandom binary sequence (PRBS)] was obtained

• BMB Reports
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• v.38 no.2
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• pp.225-231
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• 2005

Acid-labile subunit (ALS) is a component of the 150-kDa insulin-like growth factor-binding protein-3 (IGFBP-3) complex, which, by sequestering the majority of IGFs-I and -II and thereby prolonging the half-life of them in plasma, serves as a circulating reservoir of IGFs in mammalian species. A pGEX-2T plasmid and a baculovirus expression constructs harboring a coding sequence for glutathione-S transferase (GST)-porcine ALS (pALS) fusion protein were expressed in BL21(DE3) E. coli and Sf9 insect cells, respectively. The expressed protein was purified by glutathione or Ni-NTN affinity chromatography, followed by cleavage of the fusion protein using Factor Xa. In addition, pALS and hIGFBP-3 were also produced in small amounts in the Xenopus oocyte expression system which does not require any purification procedure. A 65-kDa pALS polypeptide was obtained following the prokaryotic expression and the enzymatic digestion, but biochemical characterization of this polypeptide was precluded because of an extremely low expression efficiency. The baculovirus-as well as Xenopus-expressed pALS exhibited the expected molecular mass of 85 kDa which was reduced into 75 and 65 kDa following deglycosylation of Asn-linked carbohydrates by Endo-F glycosidase, indicating that the expressed pALS was properly glycosylated. Moreover, irrespective of the source of pALS, the recombinant pALS and hIGFBP-3 formed a 130-kDa binary complex which could be immunoprecipitated by anti-hIGFBP-3 antibodies. Collectively, results indicate that an authentic pALS protein can be produced by the current expression systems.

• The Plant Pathology Journal
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• v.30 no.4
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• pp.384-396
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• 2014

Vegetative compatibility groups (VCGs) are determined for many fungi to test for the ability of fungal isolates to undergo heterokaryon formation. In several fungal plant pathogens, isolates belonging to a VCG have been shown to share significantly higher genetic similarity than those of different VCGs. In this study we sought to examine the relationship between VCG and genetic similarity of an important cool season turfgrass pathogen, Sclerotinia homoeocarpa. Twenty-two S. homoeocarpa isolates from the Midwest and Eastern US, which were previously characterized in several studies, were all evaluated for VCG using an improved nit mutant assay. These isolates were also genotyped using 19 microsatellites developed from partial genome sequence of S. homoeocarpa. Additionally, partial sequences of mitochondrial genes cytochrome oxidase II and mitochondrial small subunit (mtSSU) rRNA, and the atp6-rns intergenic spacer, were generated for isolates from each nit mutant VCG to determine if mitochondrial haplotypes differed among VCGs. Of the 22 isolates screened, 15 were amenable to the nit mutant VCG assay and were grouped into six VCGs. The 19 microsatellites gave 57 alleles for this set. Unweighted pair group methods with arithmetic mean (UPGMA) tree of binary microsatellite data were used to produce a dendrogram of the isolate genotypes based on microsatellite alleles, which showed high genetic similarity of nit mutant VCGs. Analysis of molecular variance of microsatellite data demonstrates that the current nit mutant VCGs explain the microsatellite genotypic variation among isolates better than the previous nit mutant VCGs or the conventionally determined VCGs. Mitochondrial sequences were identical among all isolates, suggesting that this marker type may not be informative for US populations of S. homoeocarpa.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.2
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• pp.21-27
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• 2018

FRN (Full range Naphtha) is distilled from crude oil in a Naphtha Splitter Unit and is separated into the Light Straight Naphtha, Heavy Naphtha, and kerosene according to the boiling point in sequence. This separation is conducted using a series of binary-like columns. In this separation method, the energy consumed in the reboiler is used to separate the heaviest components and most of this energy is discarded as vapor condensation in the overhead cooler. In this study, the first two columns of the separation process are replaced with the Petlyuk column. A structural design was exercised by a stage to stage computation with an ideal tray efficiency in the equilibrium condition. Compared to the performance of a conventional system of 3-column model, the design outcome indicates that the procedure is simple and efficient because the composition of the liquid component in the column tray was designed to be similar to the equilibrium distillation curve. An analysis of the performance of the new process indicated an energy saving of 12.3% under same total number of trays and with a saving of the initial investment cost.

• Journal of KIISE:Computer Systems and Theory
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• v.27 no.3
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• pp.235-244
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• 2000

This paper addresses a new method for constructing surface representation of 3D structures from a sequence of tomographic cross-sectional images, Firstly, we propose cell-boundary representation by transforming the cuberille space into cell space. A cell-boundary representation consists of a set of boundary cells with their 1-voxel configurations, and can compactly describe binary volumetric data. Secondly, to produce external surface from the cell-boundary representation, we define 19 modeling primitives (MP) including volumetric, planar and linear groups. Surface polygons are created from those modeling primitives using a simple table look-up operation. Comparing with previous method such as Marching Cube or PVP algorithm, our method is robust and does not make any crack in resulting surface model. Hardware implementation is expected to be easy because our algorithm is simple(scan-line), efficient and guarantees data locality in computation time.

• The KIPS Transactions:PartB
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• v.19B no.1
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• pp.19-26
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• 2012

The rapid increase of information imposes new demands of content management. The purpose of automatic audio segmentation and classification is to meet the rising need for efficient content management. With this reason, this paper proposes a high-accuracy algorithm that segments audio signals and classifies them into different classes such as speech, music, silence, and environment sounds. The proposed algorithm utilizes support vector machine (SVM) to detect audio-cuts, which are boundaries between different kinds of sounds using the parameter sequence. We then extract feature vectors that are composed of statistical data and they are used as an input of fuzzy c-means (FCM) classifier to partition audio-segments into different classes. To evaluate segmentation and classification performance of the proposed SVM-FCM based algorithm, we consider precision and recall rates for segmentation and classification accuracy for classification. Furthermore, we compare the proposed algorithm with other methods including binary and FCM classifiers in terms of segmentation performance. Experimental results show that the proposed algorithm outperforms other methods in both precision and recall rates.

• Molecules and Cells
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• v.40 no.11
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• pp.828-836
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• 2017

Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments using monocot plants. We constructed different fluorescent-tagged markers to visualize rice cell organelles, i.e., nucleus, plastids, mitochondria, peroxisomes, golgi body, endoplasmic reticulum, plasma membrane, and tonoplast, with four different fluorescent proteins (FPs) (G3GFP, mRFP, YFP, and CFP). Visualization of FP-tagged markers in their respective compartments has been reported for dicot and monocot plants. The comparative localization of the nucleus marker with a nucleus localizing sequence, and the similar, characteristic morphology of mCherry-tagged Arabidopsis organelle markers and our generated organelle markers in onion cells, provide further evidence for the correct subcellular localization of the Oryza sativa (rice) organelle marker. The set of eight different rice organelle markers with four different FPs provides a valuable resource for determining the subcellular localization of newly identified proteins, conducting co-localization assays, and generating stable transgenic localization in monocot plants.

• Journal of IKEEE
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• v.10 no.2 s.19
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• pp.103-108
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• 2006

This paper proposed a low power resource allocation algorithm for the minimum switching activity of operators in high level synthesis. In this paper, the proposed method finds switching activity in circuit each functional unit exchange for binary sequence length and value bit are logic one value. To use the switching activity was found the allocation with minimal power consumption, the proposed method visits all control steps one by one and determines the allocation with minimal power consumption at each control step. As the existing method, the execution time can be fast according to use the number of operator and maximal control step. And it is the reduction effect from 8.5% to 9.3%.

• Journal of IKEEE
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• v.10 no.2 s.19
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• pp.134-140
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• 2006

This paper proposed a low power resource allocation algorithm for the minimum switching activity of operators in high level synthesis. In this paper, the proposed method finds switching activity in circuit each functional unit exchange for binary sequence length and value bit are logic one value. To use the switching activity was found the allocation with minimal power consumption, the proposed method visits all control steps one by one and determines the allocation with minimal power consumption at each control step. As the existing method, the execution time can be fast according to use the number of operator and maximal control step. And it is the reduction effect from 8.5% to 9.3%.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.44 no.2
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• pp.127-138
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• 2007

In this paper, we propose a new X-masking scheme for utilizing logic built-in self-test The new scheme exploits the phase-shifting network which is based on the shift-and-add property of maximum length pseudorandom binary sequences(m-sequences). The phase-shifting network generates mask-patterns to multiple scan chains by appropriately shifting the m-sequence of an LFSR. The number of shifts required to generate each scan chain mask pattern can be dynamically reconfigured during a test session. An iterative simulation procedure to synthesize the phase-shifting network is proposed. Because the number of candidates for phase-shifting that can generate a scan chain mask pattern are very large, the proposed X-masking scheme reduce the hardware overhead efficiently. Experimental results demonstrate that the proposed X-masking technique requires less storage and hardware overhead with the conventional methods.