• BMB Reports
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• v.52 no.8
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• pp.475-481
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• 2019

The evolution of genome editing technology based on CRISPR (clustered regularly interspaced short palindromic repeats) system has led to a paradigm shift in biological research. CRISPR/Cas9-guide RNA complexes enable rapid and efficient genome editing in mammalian cells. This system induces double-stranded DNA breaks (DSBs) at target sites and most DNA breakages induce mutations as small insertions or deletions (indels) by non-homologous end joining (NHEJ) repair pathway. However, for more precise correction as knock-in or replacement of DNA base pairs, using the homology-directed repair (HDR) pathway is essential. Until now, many trials have greatly enhanced knock-in or substitution efficiency by increasing HDR efficiency, or newly developed methods such as Base Editors (BEs). However, accuracy remains unsatisfactory. In this review, we summarize studies to overcome the limitations of HDR using the CRISPR system and discuss future direction.

• Clinical and Experimental Pediatrics
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• v.64 no.6
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• pp.269-279
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• 2021

Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR-Cas9) is an ancient prokaryotic defense system that precisely cuts foreign genomic DNA under the control of a small number of guide RNAs. The CRISPR-Cas9 system facilitates efficient double-stranded DNA cleavage that has been recently adopted for genome editing to create or correct inherited genetic mutations causing disease. Congenital heart disease (CHD) is generally caused by genetic mutations such as base substitutions, deletions, and insertions, which result in diverse developmental defects and remains a leading cause of birth defects. Pediatric CHD patients exhibit a spectrum of cardiac abnormalities such as septal defects, valvular defects, and abnormal chamber development. CHD onset occurs during the prenatal period and often results in early lethality during childhood. Because CRISPR-Cas9-based genome editing technology has gained considerable attention for its potential to prevent and treat diseases, we will review the CRISPR-Cas9 system as a genome editing tool and focus on its therapeutic application for CHD.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.11a
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• pp.363-367
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• 2002

Represent of mathematical formula used within system handling document that is nonstructural in existent document editing system that is used in electron document processing that use computer is represented or processed by method that is nonstructural of image or text or etc. Such mathematical formular causes relative inconvenience to readablility and reusability of document and processing and exchange of document. Therefore, document editing system is required that can overcome such nonadvantage and apply MathML mathematical formula structure on efficiently structural document. Therefore, designed and implemented that document editing system for structural document creation of XML base that can mathematical formular editing of MathML base in this paper.

• BMB Reports
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• v.57 no.1
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• pp.30-39
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• 2024

Directed evolution (DE) of desired locus by targeted random mutagenesis (TRM) tools is a powerful approach for generating genetic variations with novel or improved functions, particularly in complex genomes. TRM-based DE involves developing a mutant library of targeted DNA sequences and screening the variants for the desired properties. However, DE methods have for a long time been confined to bacteria and yeasts. Lately, CRISPR/Cas and DNA deaminase-based tools that circumvent enduring barriers such as longer life cycle, small library sizes, and low mutation rates have been developed to facilitate DE in native genetic environments of multicellular organisms. Notably, deaminase-based base editing-TRM (BE-TRM) tools have greatly expanded the scope and efficiency of DE schemes by enabling base substitutions and randomization of targeted DNA sequences. BE-TRM tools provide a robust platform for the continuous molecular evolution of desired proteins, metabolic pathway engineering, creation of a mutant library of desired locus to evolve novel functions, and other applications, such as predicting mutants conferring antibiotic resistance. This review provides timely updates on the recent advances in BE-TRM tools for DE, their applications in biology, and future directions for further improvements.

• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.107-114
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• 2017

Implementation of crop improvement programs relies on genetic diversity. To overcome the limited occurrence of natural mutations, researchers and breeders applied diverse methods, ranging from conventional crossing to classical bio-technologies. Earlier generations of knockout and gain-of-function technologies often result in incomplete gene disruption or random insertions of transgenes into plant genomes. The newly developed editing tool, CRISPR/Cas9 system, not only provides a powerful platform to efficiently modify target traits, but also broadens the scope and prospects of genome editing. Customized Cas9/guide RNA (gRNA) systems suitable for efficient genomic modification of mammalian cells or plants have been reported. Following successful demonstration of this technology in mammalian cells, CRISPR/Cas9 was successfully adapted in plants, and accumulating evidence of its feasibility has been reported in model plants and major crops. Recently, a modified version of CRISPR/Cas9 with added novel functions has been developed that enables programmable direct irreversible conversion of a target DNA base. In this review, we summarized the milestone applications of CRISPR/Cas9 in plants with a focus on major crops. We also present the implications of an improved version of this technology in the current plant breeding programs.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.05a
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• pp.277-280
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• 2003

Established XML at February, 1998 in W3C by solution about document processing and exchange and reusability to be shortcoming that early web happens using nonstructural document. Existing electron transaction is changing in electronic business form between corporation through XML base message exchange using XML. Necessity about solution that can masticate structured electron transaction of XML base that is used in electron transaction between corporation rose. Structured electron transaction of XML base that is used in electron transaction in treatise that see hereupon efficiently study about XML document editing system that integrate XML Schema editor to masticate XML Schema document that define edit and XML instance editor of user central that can write a book and structure of XML document efficiently do.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.3
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• pp.513-518
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• 2003

Established XML at February, 1998 in W3C by solution about document processing and exchange and reusability to be shortcoming that early web happens using nonstructural document. Existing electron transaction is changing in electronic business form between corporation through XML base message exchange using XML. Necessity about solution that can masticate structured electron transaction of XML base that is used in electron transaction between corporation rose. Structured electron transaction of XML base that is used in electron transaction in treatise that see hereupon efficiently study about XML document editing system that integrate XML Schema editor to masticate XML Schema document that define edit and XML instance editor of user central that can write a book and structure of XML document efficiently do.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.5
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• pp.843-850
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• 2006

The standardization on RDD and REL has been developed by MPEG. REL is a Right Expression Language and RDD is term dictionary which develop active application of REL. However, since REL documents could be oかy edited by user understanding MPEG-21's framework, much easier editing system is required. As well, REL Consumption System to process and analyze REL documents, the RDD interoperability system to interoperate REL and RDD are needed. In this paper, REL Editing System and REL Consumption System, RDD Web Services System were designed and implemented, REL Editing System to make REL document, REL Consumption System to process and analyze edited documents, RDD Web Services System to process rights inquiry base on the Web Services.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.903-920
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• 2020

Since its first demonstration as a practical genome editing tool in the early 2010s, the use of clustered regularly interspaced short palindromic repeat (CRISPR) along with the endonuclease Cas9 (CRISPR/Cas9) has become an essential choice for generating targeted mutations. Due to its relative simplicity and cost-effectiveness compared to other molecular scissors, i.e., zinc finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN), the CRISPR/Cas9 system has been shown to have a massive influence on genetic studies regardless of the biological kingdom. Although the system is in the process of being established, numerous protocols have already been released for the system and there have been various topics of CRISPR related papers published each year in ever-increasing manner. Here, we will briefly introduce CRISPR/Cas9 system and discuss the variants of the CRISPR system. Also, their applications to crop improvement will be dealt with mainly ornamental crops among horticultural crops other than Arabidopsis as a model plant. Finally, some issues on the barriers restraining the use of CRISPR system on floricultural crops, the prospect of CRISPR system as a DNA-free genome editing tool with efficient facilitators and finally, the future perspectives on the CRISPR system will be described.

• Genomics & Informatics
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• v.5 no.1
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• pp.30-31
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• 2007

Many visualization tools have been designed to aid information processing during whole genome projects. We have developed a trace viewer program, ChroView, which can read a chromatogram file and display the chromatogram traces of the four bases. The program can be used to examine sequencing quality and base-calling errors. It can also help researchers to edit and save base-calling results while browsing the traces. Additionally, this program has a basecalling feature which can produce supplementary data for validation of the results from other base-calling programs.