• Journal of Animal Reproduction and Biotechnology
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• v.39 no.1
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• pp.2-11
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• 2024

Background: The small intestine plays a crucial role in animals in maintaining homeostasis as well as a series of physiological events such as nutrient uptake and immune function to improve productivity. Research on intestinal organoids has recently garnered interest, aiming to study various functions of the intestinal epithelium as a potential alternative to an in vivo system. These technologies have created new possibilities and opportunities for substituting animals for testing with an in vitro model. Methods: Here, we report the establishment and characterisation of intestinal organoids derived from jejunum tissues of adult pigs. Intestinal crypts, including intestinal stem cells from the jejunum tissue of adult pigs (10 months old), were sequentially isolated and cultivated over several passages without losing their proliferation and differentiation using the scaffold-based and three-dimensional method, which indicated the recapitulating capacity. Results: Porcine jejunum-derived intestinal organoids showed the specific expression of several genes related to intestinal stem cells and the epithelium. Furthermore, they showed high permeability when exposed to FITC-dextran 4 kDa, representing a barrier function similar to that of in vivo tissues. Collectively, these results demonstrate the efficient cultivation and characteristics of porcine jejunum-derived intestinal organoids. Conclusions: In this study, using a 3D culture system, we successfully established porcine jejunum-derived intestinal organoids. They show potential for various applications, such as for nutrient absorption as an in vitro model of the intestinal epithelium fused with organ-on-a-chip technology to improve productivity in animal biotechnology in future studies.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.40 no.1
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• pp.59-73
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• 2003

As a means of overcoming the linguistic barrier between different languages in the Internet, a new sign-language communication system with CG animation techniques has been developed and proposed. In the system, the joint angles of the arms and the hands corresponding to the gesture as a non-verbal communication tool have been considered. The emotional expression, however, could as play also an important role in communicating each other. Especially, a comic expression is more efficient than real facial expression, and the movements of the cheeks and the jaws are more important AU's than those of the eyebrow, eye, mouth etc. Therefore, in this paper, we designed a 3D emotion editor using 2D model, and we extract AU's (called as PAU, here) which play a principal function in expressing emotions. We also proposed a method of generating the universal emotional expression with Avatar models which have different vertex structures. Here, we employed a method of dynamically adjusting the AU movements according to emotional intensities. The proposed system is implemented with Visual C＋＋ and Open Inventor on windows platforms. Experimental results show a possibility that the system could be used as a non-verbal communication means to overcome the linguistic barrier.

• Wind and Structures
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• v.17 no.2
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• pp.203-225
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• 2013

An analysis framework for vehicle-bridge dynamic interaction system under turbulent wind is proposed based on the relevant theory of wind engineering and dynamics. Considering the fluctuating properties of wind field, the stochastic wind velocity time history is simulated by the Auto-Regressive method in terms of power spectral density function of wind field. The bridge is represented by three-dimensional finite element model and the vehicle by a multi-rigid-body system connected by springs and dashpots. The detailed calculation formulas of unsteady aerodynamic forces on bridge and vehicle are derived. In addition, the form selection of wind barriers, which are applied as the windbreak measures of newly-built railways in northwest China, is studied based on the suggested evaluation index, and the suitable values about height and porosity rate of wind barriers are studied. By taking a multi-span simply-supported box-girder bridge as a case study, the dynamic response of the bridge and the running safety indices of the train traveling on the bridge with and without wind barriers are calculated. The limit values of train speed with respect to different wind velocities are proposed according to the allowance values in the design code.

• Applied Science and Convergence Technology
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• v.25 no.4
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• pp.81-84
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• 2016

InP/InGaP quantum structures (QSs) grown on GaAs substrates by a migration-enhanced molecular beam epitaxy method were studied as a function of growth temperature (T) using photoluminescence (PL) and emission-wavelength-dependent time-resolved PL (TRPL). The growth T were varied from $440^{\circ}C$ to $520^{\circ}C$ for the formation of InP/InGaP QSs. As growth T increases from $440^{\circ}C$ to $520^{\circ}C$, the PL peak position is blue-shifted, the PL intensity increases except for the sample grown at $520^{\circ}C$, and the PL decay becomes fast at 10 K. Emission-wavelength-dependent TRPL results of all QS samples show that the decay times at 10 K are slightly changed, exhibiting the longest time around at the PL peak, while at high T, the decay times increase rapidly with increasing wavelength, indicating carrier relaxation from smaller QSs to larger QSs via wetting layer/barrier. InP/InGaP QS sample grown at $460^{\circ}C$ shows the strongest PL intensity at 300 K and the longest decay time at 10 K, signifying the optimum growth T of $460^{\circ}C$.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.52 no.10
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• pp.476-480
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• 2003

In this paper, we have performed 3-dimensional time-resolving measurement of the Ne light emitted from the cell of plasma display panel(PDP) as a function of the pressure using the scanned point detecting system. From the temporal behavior results, we could analyze the discharge behavior of panel with Ne-Xe(4%) mixing gas and 300 torr, 400 torr and 500 torr pressure. At the top view of panel, the discharge of 300 torr panel starts at the 634 ns and ends at the 722 ns. The emission duration time is about 90 ns. The discharge of 400 torr panel starts at the 682 ns and ends at the 786 ns. the emission duration time is about 100 ns. Also, the discharge of 500 torr panel starts at the 770 ns and ends at the 826 ns. the emission duration time is about 56 ns. The discharge moves from inner edge of cathode electrode to outer cathode electrode forming arc type. In the side view of 300 torr, 400 torr and 500 torr an emission shows that the light is detected up to 180${\mu}{\textrm}{m}$, 150${\mu}{\textrm}{m}$ and 70${\mu}{\textrm}{m}$ height of barrier rib and the emission distribution of the 300 torr is wider than 400 torr, 500 torr.

• Nuclear Engineering and Technology
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• v.48 no.1
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• pp.218-227
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• 2016

Background: Steam explosions may occur in nuclear power plants by molten fuel-coolant interactions when the external reactor vessel cooling strategy fails. Since this phenomenon can threaten structural barriers as well as major components, extensive integrity assessment research is necessary to ensure their safety. Method: In this study, the influence of yield criteria was investigated to predict the failure of a reactor cavity under a typical postulated condition through detailed parametric finite element analyses. Further analyses using a geometrically simplified equivalent model with homogeneous concrete properties were also performed to examine its effectiveness as an alternative to the detailed reinforcement concrete model. Results: By comparing finite element analysis results such as cracking, crushing, stresses, and displacements, the Willam-Warnke model was derived for practical use, and failure criteria applicable to the reactor cavity under the severe accident condition were discussed. Conclusion: It was proved that the reactor cavity sustained its intended function as a barrier to avoid release of radioactive materials, irrespective of the different yield criteria that were adopted. In addition, from a conservative viewpoint, it seems possible to employ the simplified equivalent model to determine the damage extent and weakest points during the preliminary evaluation stage.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2007.11a
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• pp.31-32
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• 2007

Built-in voltage in organic light-emitting diodes was studied using modulated photocurrent technique ambient conditions. A device was made with a structure of anode/$Alq_3$/cathode to study a built-in voltage. An ITO was used as an anode, and $Li_2O$/Al was used as a cathode. From the bias voltage-dependent photocurrent, built-in voltage of the device is determined. The applied bias voltage when the magnitude of modulated photocurrent is zero corresponds to a built-in voltage. Built-in voltage in the device is generated due to a difference of work function of the anode and cathode. It was found that for 0.5nm thick $Li_2O$ layer built-in voltage is the higher than the others. It indicates that a very thin alkaline metal compound $Li_2O$ lowers an electron barrier height.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.23 no.4
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• pp.388-396
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• 2020

Purpose: Impaired intestinal mucosal integrity may affect the gastrointestinal function, especially in relation to nutrition, absorption, and barrier function. The purpose of this study was to measure the prevalence of impaired intestinal mucosal integrity in presumed healthy children aged 1-3 years and assess the effects of zinc, glutamine, fiber, and prebiotic supplementation in them. Methods: A cross-sectional study was conducted in 200 children aged 1-3 years in Pasar Minggu, South Jakarta, Indonesia. A randomized double-blind parallel group method clinical trial was then performed to assess the effects of zinc, glutamine, fiber, and prebiotic supplementation. Results: Elevated calprotectin was found in 91/200 subjects (45.5%) at the onset of the study. After 10 months, 144 subjects completed the study: 72 subjects received the trial formula, whereas the other 72 received the standard formula. A transitory decrease in fecal calprotectin (FC) was observed after 6 months in the subgroup with normal FC levels, who were fed the test formula (p=0.012). Conclusion: The prevalence of impaired intestinal mucosal integrity in this group of Indonesian children aged 1-3 years was high. Supplementation with zinc, glutamine, fiber, and prebiotics during 6 months reduced FC only in those who had low levels at baseline but not in those with impaired integrity.

• Journal of Industrial Technology
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• v.23 no.A
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• pp.199-206
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• 2003

The charcoal-encapsulated methyl silica microcapsules were prepared by a O/W microemulsion sol-gel method, and the adsorption properties on aquatic humic acid were investigated. The capsules prepared were spherical, $100{\sim}1000{\mu}m$ in size. The size distribution was controllable by adjusting the size of charcoal powder, charcoal/methyl silica ratio, and agitating speed in O/W sol-gel process. Adsorption efficiency of charcoal for aquatic humic acid was decreased after encapsulation by methyl silica shell. The decreased adsorption efficiency can be dependent on the decrease of the BET surface area and pore volume after encapsulation. Diffusion properties of humic acid through the capsule shell also played an important role on adsorption efficiency. Therefore, the reasonable target pollutants for the capsules can be VOC or odor molecules which can overcome diffusion barrier through shell of capsules in air condition. Functionalization methods for the charcoal-encapsulated $CH_3(SiO)_n$ microcapsules by incorporation of $TiO_3$ as a phtocatalytic function and by incorporation of inorganic pigment as a color function were also investigated. $TiO_2$ coating properties were controllable by adjusting pH, temperature, and the concentration of $TiOSO_4$. In XRD measurement, the crystal form of the coated $TiO_2$ was anatase. For the colorization of the capsules, inorganic pigments were more efficient than organic dyes, and various color was introduced to the capsules using inorganic pigments.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.04b
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• pp.14-16
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• 2008

Built-in voltage in organic light-emitting diodes was studied using modulated photocurrent technique ambient conditions. From the bias voltage-dependent photocurrent, built-in voltage of the device is determined. The applied bias voltage when the magnitude of modulated photocurrent is zero corresponds to a built-in voltage. Built-in voltage in the device is generated due to a difference of work function of the anode and cathode. A device was made with a structure of anode/$Alq_3$/cathode to study a built-in voltage. ITO was used as an anode, and Al and LiAl were used as a cathode. A layer thickness of Al and LiAl were 100nm. Obtained built-in voltage is about 1.0V in the Al layer was used as a cathode. The obatined built-in voltage is about 1.6V in the LiAl layer was used as a cathode. The result of built-in voltage is dependent of cathode. We can see that the built-in voltage increase up to 0.4V when the LiAl layer was used as the cathode. These results correspond to the work function of LiAl which is lower than that of Al. As a result, the barrier height for an electron injection from the cathode to the organic layer could be lowered when the LiAl was used as a cathode.