• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.30 no.4
• /
• pp.49-61
• /
• 2017

Objectives : In order to find out the whitening effects of Leonuri Herba, this study was designed to identify the effects and the action mechanism of LHE(Leonuri Herba extract) on Malignant melanoma cell lines. Methods : After treating LHE on the B16F10 cell-Malignant melanoma cell line-, the cell survival rate, melanin biosynthesis rate, intra&extracellular tyrosinase activity rate, SOD-like activity, tyrosinase mRNA gene expression were investigated. The results were compared with control group without LHE treatment or with positive control group treated with whitening efficacy substance. Results : B16F10 cell survival rate, melanin biosynthesis rate, and intra&extracellular tyrosinase activity were significantly inhibited depending on the concentration of treated LHE. Melanin biosynthesis rate and tyrosinase activity rate were also decreased when ${\alpha}-MSH$ was combined with LHE. In addition, the SOD-like activity was increased in a concentration-dependent manner in the treatment with the LHE, indicating signigicant activity at high concentrations, and the tyrosinase mRNA gene expression was decreased in both the LHE-treated group, the LHE and ${\alpha}-MSH-treated$ group. Conclusions : LHE seems to inhibit melanin synthesis through inhibition of tyrosinase activity and inhibition of tyrosinase mRNA gene expression. It also has the effect of promoting SOD-like activity and may be used clinically as a skin whitening agent in the future.

• Animal cells and systems
• /
• v.10 no.4
• /
• pp.233-236
• /
• 2006

For the purpose of the development of skin-whitening or therapeutic agents against hyperpigmentation, aqueous extract from Nardostachys chinensis (AENC) was evaluated for melanogenesis inhibitory activity in B16F10 melanoma cell. The treatment with AENC at the 0.2, 0.5 and 1.0 mg/ml level significantly inhibits the biosynthesis of melanin compared with untreated control. The tyrosinase activity also significantly decreased in AENC-treated cells at the 0.2 and 0.5 mg/ml level and inhibitory effects were more efficient than commercial arbutin at 0.1 mg/ml. The Western analyses confirmed the significantly decreased expression of tyrosinase and tyrosinase-related protein-2 by AENC treatment. These results indicate that AENC may contribute to the inhibition of melanin biosynthesis through regulating the expression as well as activity of tyrosinase and AENC may be useful as a new candidate in the design of new skinwhitening or therapeutic agents.

• KSBB Journal
• /
• v.19 no.6 s.89
• /
• pp.437-440
• /
• 2004

To determine the inhibition of tyrosinase activity and melanogenesis, gardenia fruit was extracted initially with $95\%$ ethanol (w/v), The ethanol extract was fractionated subsequently with hexane, chloroform and ethyl acetate in that order. The inhibitory effect of the ethanol extract on tyrosinase activity was higher than water extract. When 10 mg/mL of ethyl acetate fraction was applied, the inhibition ratio of tyrosinase activity was much higher ($99.7{\pm}0.1\%$) than that of arbutin. The inhibition of melanogenesis using B16F10 melanoma cell, the ethanol extract also showed higher inhibitory effect than water extract. The highest inhibitory activity of melanogenesis was also shown in ethyl acetate fraction ($89.1{\pm}1.4\%$ at the cone. of $100\;{\mu}g/mL$). These results suggests that gardenia extracts might be used to be a potential agents for whitening.

• KSBB Journal
• /
• v.28 no.3
• /
• pp.196-201
• /
• 2013

Silkworm-derived silk materials which contain 45 to 55% protein (18 amino acids, including 8 essential amino acids) have free radical-scavenging activities. In the present study, we investigated the whitening and anti-aging effects of silkworm gland under various conditions with B16F1 melanoma cells and human dermal fibroblasts. To evaluate the toxicity of soluble gland hydrolysate (SGH), mouse-derived B16F1 melanoma cells were treated with 0.2~5 mg/mL, cytotoxicity was not observed at all concentrations. The tyrosinase and elastase activities were significantly decreased as dosage levels of SGH increased. In addition, cell death was decreased by treated with SGH and antioxidative activity was the most effective in the SGH treatment. These findings suggest that SGH may be used as a potential functional biomaterial in having the skin whitening effect and anti-aging activity by inhibition of tyrosinase and elastase activities.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.18 no.3
• /
• pp.747-753
• /
• 2004

Effects of methanolic extract from Adenophorae Radix (AR) on melanogenesis were investigated in mouse melanoma B16F10 cells. The methanol extract of AR was partitioned into Hexane, Ethyl acetate (EA), Butanol, H₂O, and exogenously added to the culture medium for 72 hours at the concentration of 10, 50, 100 and 200 ㎍/㎖. Of the four partitions, Hexane and EA partion of AR reduced tyrosinase activity, which is the key enzyme for a melanogenesis, as well as melanin contents. But the EA partition was less toxic for B16F10 cells and has more efficient melanin-reducing effect than the former. In addition, the EA partition dramatically lightened the color of cell pellet and significantly decreased the level of tyrosinase protein expression. In these results, EA partition of AR reduced melanin synthesis of B16F10 mouse melanoma cells by down regulating the tyrosinase activity and tyrosinase protein expression. Therefore, it is anticipated that AR is a candidate for an efficient whitening agent which supresses melanogenesis.

• Journal of Applied Biological Chemistry
• /
• v.54 no.4
• /
• pp.284-289
• /
• 2011

The extracts of Agrimonia pilosa L. were investigated for the inhibitory effect on the melanin synthesis in B16/F10 mouse melanoma cells as a functional ingredient for cosmetic products. Tyrosinase inhibition activities were 42% in A. pilosa L. 70% ethanol extract at $500{\mu}g/mL$. The protein and mRNA expression of tyrosinase, which are all skin-whitening related factors, showed that A. pilosa L. water and A. pilosa L. 70% ethanol extracts inhibited the protein bio-synthesis in B16F10 melanoma cell. Results indicate that the A. pilosa L. extracts tested in the present study have skin whitening activity and can be used as a functional ingredient for cosmetic compositions.

• Korean Journal of Food Science and Technology
• /
• v.50 no.6
• /
• pp.660-664
• /
• 2018

Honey is known to have a variety of health-promoting effects. Ethyl acetate fractions (EAFs) of four Korean domestic honeys from basswood, Korean raisin, chestnut, and acacia as well as a New Zealand manuka honey were analyzed comparatively to evaluate their antioxidative and antimelanogenic effects. The EAFs of basswood, Korean raisin, and chestnut honey had higher antioxidant capacities and tyrosinase inhibition activities than those of manuka honey. Pretreatment of B16F1 melanoma cells with EAFs at $100{\mu}g/mL$ resulted in relative retention of melanin contents as follows: acacia honey (141.0%)>basswood honey (134.3%)>manuka honey (131.5%)>Korean raisin honey (107.3%)>chestnut honey (88.0%). Intracellular tyrosinase activities of B16F1 melanoma cells were significantly (p<0.05) lowered by EAFs of Korean raisin and chestnut honey than by EAF of manuka honey. Consequently, these results suggest that Korean domestic honeys from different floral sources serve as good sources of antioxidative and antimelanogenic agents.

• YAKHAK HOEJI
• /
• v.45 no.6
• /
• pp.724-729
• /
• 2001

Melanogenesis is a physiological process resulted in the synthesis of melanin pigments, which has a role in protecting skin front the damaging effect of ultra-violet (UV) radiation. The main aim of the present study was to examine the effect of Ulmus davidiana var. japonica(UL) on Melanogenesis. Cells were cultured in the presence of various concentrations of Ulmus davidiana var. japonica for 48 h, and there were estimated total melanin contents as a final product and activity of tyrosinase, a key enzyme, in Melanogenesis. Among the four solvent extracts tested, EtOAc extract mostly increased tyrosinase activity, And EtOAc extract increased the melanin contents and tyrosinase activity in a dose-dependent manner. Especially It was observed that 100$\mu\textrm{g}$/ml EtOAc extract promotes melanin secretion in B16/F10 melanoma cells by 140% at 48 h treatment and activity of tyrosinase increased by 180% in the presence of same concentration. In conclusion, as for EtOAc extract treatment, there was no effect on the viability of B16/F10 cell, only to stimulate Melanogenesis.

• Microbiology and Biotechnology Letters
• /
• v.41 no.4
• /
• pp.407-415
• /
• 2013

In this study, we investigated the antioxidant activities on HaCaT and the whitening effects on B16F1 melanoma cells of Dendropanax morbifera leaf extract. In an antioxidative activity assay using HaCaT cells, the ethyl acetate ($50{\mu}g/ml$) and aglycone fractions ($25{\mu}g/ml$) of the D. morbifera leaf extract didn't exhibit any characteristics of cytotoxicity. When HaCaT cells were exposed to a single large dose ($800mJ/cm^2$) of UVB, the extracts protected the cells against UVB radiation. When HaCaT cells were treated with 10 mM $H_2O_2$ and $4{\mu}M$ rose bengal, the ethyl acetate ($6.25{\sim}50{\mu}g/ml$) and aglycone ($6.25{\sim}25{\mu}g/ml$) fractions protected the cells against oxidative damage in a concentration dependent manner. When the whitening effects of D. morbifera leaf extract were tested in melanoma B16/F1 cells treated with the a-melanocyte stimulating hormone (${\alpha}$-MSH), the extracts inhibited ${\alpha}$-MSH-stimulated intra/extracellular melanogenesis in a concentration dependent manner. The inhibitory effects of the ethyl acetate and aglycone fractions of D. morbifera leaf extract were 21% and 44% at $25{\mu}g/ml$, respectively. Both are more effective than arbutin (15% at $25{\mu}g/ml$) which is known as a whitening agent. These results indicate that fractions of the D. morbifera leaf can function as cell protectants and natural antioxidants in biological systems, particularly skins exposed to UV radiation by quenching and/or scavenging $^1O_2$ and other ROS, and protecting cells against ROS. In addition, fractions of the D. morbifera leaf can be applied to new whitening cosmetics because of their inhibitory effects on ${\alpha}$-MSH stimulated melanogenesis in B16F1 melanoma cells.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.23 no.2
• /
• pp.337-342
• /
• 2009

Acquired pigmentary skin diseases such as abnormal melanogenesis, vitiligo, chloasma and inflammatory pigmentation are related to regulate the melanin production, In this study, an ethanol extract of Hovenia dulcis Thunb.(EHD) makedly inhibited melanin biosynthesis and suppressed, the protein expression of tyrosinase, tyrosinase-related protein 1(TRP-1), and tyrosinase-related protein 2(TRP-2) in B16F10 cells. On the other hand, EHD did not inhibit mushroom tyrosinase activity. These results indicate that EHD may contribute to the inhibition of melanin biosynthesis through regulating tyrosinase activity and expression, and serve as a new candidate in the design of new skin-whitening or therapeutic agents.