Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Whitening Activities of the Agrimonia pilosa L. Extracts

선학초 추출물의 미백활성

  • Kim, Dong-Hee (Daegu Gyeongbuk Institute for Oriental Medicine Industry) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University)
  • 김동희 ((재)대구경북한방산업진흥원) ;
  • 안봉전 (대구한의대학교 화장품약리학과) ;
  • 이진영 (호서대학교 한방화장품과학과)
  • Received : 2011.10.13
  • Accepted : 2011.11.02
  • Published : 2011.12.31
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Abstract

The extracts of Agrimonia pilosa L. were investigated for the inhibitory effect on the melanin synthesis in B16/F10 mouse melanoma cells as a functional ingredient for cosmetic products. Tyrosinase inhibition activities were 42% in A. pilosa L. 70% ethanol extract at $500{\mu}g/mL$. The protein and mRNA expression of tyrosinase, which are all skin-whitening related factors, showed that A. pilosa L. water and A. pilosa L. 70% ethanol extracts inhibited the protein bio-synthesis in B16F10 melanoma cell. Results indicate that the A. pilosa L. extracts tested in the present study have skin whitening activity and can be used as a functional ingredient for cosmetic compositions.

선학초 추출물을 기능성 화장품 소재로 활용하기 위하여 미백효과를 검증하였다. 세포 내 tyrosinase 저해활성 측정결과 선학초 에탄올 추출물 $500{\mu}g/mL$에서 42%의 저해활성을 나타내었다. 이는 선학초 추출물이 세포 내 tyrosinase 발현을 억제시킴으로서 멜라닌 합성 또한 저해 하는 것임을 확인 할 수 있었다. 선학초 추출물의 단백질 발현과 mRNA 발현 억제효과를 검토한 결과 선학초 열수 및 에탄올 추출물을 처리한 B16F10군에서는 tyrosinase protein의 발현이 처리하지 않은 군보다 감소함을 확인할 수 있었다. 결과적으로 선학초 추출물의 미백효능을 확인할 수 있었으며, 식품 및 화장품의 기능성 소재로 이용이 가능할 것으로 판단된다.

Keywords

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