The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
권호 표지
DOI QR코드

DOI QR Code

Skin Whitening Effect of Leonuri Herba Extracts(LHE) on Malignant Melanoma Cell

익모초(益母草) 추출물이 악성 흑색종 세포에 미치는 피부미백효과

  • Kim, Ji-Eun (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Lim, Kyeong-Min (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Na, Ga-yeong (Department of Internal medicine, College of Korean Medicine, Dong-shin University) ;
  • Kim, Hye-Hwa (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Kang, Byung-Soo (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Choi, Jung-Hwa (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Park, Soo-Yeon (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Jung, Min-Yeong (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University)
  • 김지은 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 임경민 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 나가영 (동신대학교 한의과대학 내과학교실) ;
  • 김혜화 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 강병수 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 최정화 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 박수연 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 정민영 (동신대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2017.10.16
  • Accepted : 2017.11.03
  • Published : 2017.11.25
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : In order to find out the whitening effects of Leonuri Herba, this study was designed to identify the effects and the action mechanism of LHE(Leonuri Herba extract) on Malignant melanoma cell lines. Methods : After treating LHE on the B16F10 cell-Malignant melanoma cell line-, the cell survival rate, melanin biosynthesis rate, intra&extracellular tyrosinase activity rate, SOD-like activity, tyrosinase mRNA gene expression were investigated. The results were compared with control group without LHE treatment or with positive control group treated with whitening efficacy substance. Results : B16F10 cell survival rate, melanin biosynthesis rate, and intra&extracellular tyrosinase activity were significantly inhibited depending on the concentration of treated LHE. Melanin biosynthesis rate and tyrosinase activity rate were also decreased when ${\alpha}-MSH$ was combined with LHE. In addition, the SOD-like activity was increased in a concentration-dependent manner in the treatment with the LHE, indicating signigicant activity at high concentrations, and the tyrosinase mRNA gene expression was decreased in both the LHE-treated group, the LHE and ${\alpha}-MSH-treated$ group. Conclusions : LHE seems to inhibit melanin synthesis through inhibition of tyrosinase activity and inhibition of tyrosinase mRNA gene expression. It also has the effect of promoting SOD-like activity and may be used clinically as a skin whitening agent in the future.

Keywords

References

  1. Kang WH. Atlas of skin disease. Seoul:Hanmi medical publishing. 2016:265.
  2. Imayama S, Miyachi Y, Matsunaga K, Utsugi R. Scientific skin care. Seoul:Dae-Young publishing. 2009:32-5.
  3. Wang KH, Lin RD, Hsu FL, Huang YH, Chang HC, Huang CY, et al. Cosmetic applications of selected traditional Chinese herbal medicines. Journal of Ethnopharmacology. 2006;106(3):353-9. https://doi.org/10.1016/j.jep.2006.01.010
  4. Prota G. Recent Advances in the Chemistry of Melanogenesis In mammals. Journal of Investigative Dermatology. 1980;75(1):122-6. https://doi.org/10.1111/1523-1747.ep12521344
  5. Textbook Compilation Committee of National University of Korean medicine. Herbalogy. Seoul:Yeong-Lim publishing. 2010:460-1.
  6. Shin MK. Clinical Traditional Herbalogy. Seoul:Yeong-Lim Publishing. 1986:355-6.
  7. Kim JY. Anti-oxidative and anti-inflammatory activity of Lenonuri Herba Extracts. University of Jungang. 2005.
  8. Kim TY, Jang SA, Chae YB, Park JP. Antioxidant and Protective Effects of Leonurus sibiricus L Extract on Ultraviolet B(UVB)-induced Damage in Human Keratinocytes. Korean Journal of Plant Resources. 2016;29(1):11-9. https://doi.org/10.7732/kjpr.2016.29.1.011
  9. Cho YK. The Development of Anti-wrinkle Cosmeceutical Ingredients from Leonuri Herba Extrat. University of Jungang. 2011.
  10. Baek JH. Anticancer and immunomodulatory effects of Leonuri Herba. University of Kyunghee. 2007.
  11. Park SC, Na HM, Bae YH, Zhou J, Na CS, Kim JS. Effect of Leonurus sibiricus on Uterine Activity. Journal of Reproductive and Developmental Biology. 1996;19(4):245-50.
  12. Seo HJ, Kook HS, Lee MY. Effecrs of Leonurus sibiricus extract on experimentally induced liver damage and alloxan diabetes in Rabbits. Journal of the Korean Society of Food Science and Nutrition. 1987;16(1):22-8.
  13. Lee SJ, Baek SH, Kim DC. The effect of Leonurus sibiricus on the proliferation inhibition of human uterine Leiomyoma cell and expression of gene related cell apoptosis. The Journal of Oriental Gynecology. 2006;19(3):1-12.
  14. Hosoi J, Abe E, Suda T, Kuroki T. Regulation of melanin synthesis of B16 mouse melanoma cells by $1{\alpha}$ 25-Dihydroxyvitamin D3 and Retinoic Acid. Cancer Research. 1985;45(4):1474-8.
  15. Yagi A, Kanbara T, Morinobu N. The effects of tyrosinase inhibition for aloe. Planta medica. 1986;53(6):517-9. https://doi.org/10.1055/s-2006-962799
  16. Martinez-Esparza M, Jimenez-Cervantes C, Solano F, Lozano JA, Garcia-Borron JC. Mechanism of melanogenesis inhibition by tumor necrosis factor-alpha in B16/F10 mouse melanoma cells. European Journal of Biochemistry. 1988;255(1):139-46. https://doi.org/10.1042/bj2550139
  17. Marklund S, Marklund G. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 1974;47(3):468-74.
  18. Kim SH, Jung H, Shin YC, Ko SG. Research of Traditional Herbal Medicines for Anti-aging, Inhibition Effect of Wrinkle and Whitening Effect in the Skin. Korean Journal of Oriental Physiology & Pathology. 2008;22(3):691-8.
  19. Sulaimon S, Kitchell B. The Biology of Melanocytes. Veterinary Dermatology. 2003;14(2):57-65. https://doi.org/10.1046/j.1365-3164.2003.00327.x
  20. Prota G. Recent advances in the chemistry of melanogenesis in mammals. Journal of investigative dermatology. 1980;75(1):122-6. https://doi.org/10.1111/1523-1747.ep12521344
  21. Shin YS, Roh SS. A literatual study on the causes and treatments of the melasma. Journal of Korean Oriental Ophthalmology & Otorhinolaryngology & Dermatology. 1998;11(1):82-98.
  22. Textbook compilation committee of National university korean medicine Dermatology & Surgery. Oriental Medical Dermatology & Surgery. Busan:Sunwo publishing. 2007: 458-60.
  23. Lee SH. Depigmenting activity of mapoongko and ephedrae herba. University of Kyunghee. 2001.
  24. Kim SK. Studies on the whitening effect test of Sabaeck-san. University of Kyunghee. 2000.
  25. Park HS, Kim HJ, Kim YB. Study on the whitening effect of Kamigyubi-tang. Journal of Korean Oriental Ophthalmology & Otorhinolaryngology & Dermatology. 2004;17(2):48-58.
  26. Lee SE, Kim HJ, Kim YB. Study on the whitening effect of Kamibangpoongtongsung- san. Journal of Korean Oriental Ophthalmology & Otorhinolaryngology & Dermatology. 2004;17(1):94-103.
  27. Park YI. The Effects of Modified Yeonryeonggobondan Preparat on Skin Care. University of Sangji. 2007.
  28. Kim JK, Yoo DY. The Effect of Yukmijihwangtang-gagambang, YMG on Melanin Synthesis and Gene Expression. The Journal Of Oriental Gynecology. 2009;22(3):66-82.
  29. An TEB, Kim DC. In Vitro Cytotoxicity, Skin Regeneration, Anti-wrinkle, Whitening and In Vivo Skin Moisturizing Effects of Oncheongeum. The Journal Of Oriental Gynecology. 2016;29(1):14-34. https://doi.org/10.15204/jkobgy.2016.29.1.014
  30. Kwak JH, Kim YH, Jang HR, Park CW, Han YH. Inhibitory Effect of Gardenia Fruit Extracts on Tyrosinase Activity and Melanogenesis. KSBB Journal. 2004;19(6):437-40.
  31. Yoo YK, Jung MS, Choi JW, Kim JH. The Study on the Whitening Effect of Ephedra sinica Extract. Journal of the society of cosmetic scientists of korea. 2005;31(2):153-9.
  32. Jung HS. Effects of Dictamni Radicis Cortex extracts on skin whitening. University of Wonkwang. 2016.
  33. Jeon SH, Lim MH. The effect of Baeckji, Seasin, Baeckgangjam and Myunkanbang on skin-whitening. Journal of The Korean Oil Chemists' Society. 2011;28(2):161-9.
  34. Choi JH, Park SY, Kim JH, Jeong MY. Effects of Lycii Fructus Extracts(LFE) on Skin whitening and Elasticity using Melanoma cells. J Korean Med Opthalmol Otolaryngol Dermatol. 2014;27(1):58-67.
  35. Heo Jun. Sinjeungbo daeyeok Donguibogam. Seoul:Bup-in publishing. 2012:600.
  36. Chen Q, Fischer A, Reagan JD, Yan LJ, Ames BN. Oxidative DNA damage and senescence of human diploid fibroblast cells. Proceedings of the National Academy of Sciences of the USA. 1995;92(10):4337-41. https://doi.org/10.1073/pnas.92.10.4337
  37. Won YH, Hur SG, Lee SC, Chunn IK, Kim YP. Clinical and histopathologic study of malignant melanoma(1980-1990). Korean Journal of Dermatology. 1991;29(2):193-202.
  38. Liu TH, Beckman JS, Freeman BA, Hogan EL, Hsu CY. Polyethylene glycol-conjugated superoxide dismutase and catalase reduce ischemic brain injury. American Journal of Physiology. 1990;256(2):H589-93.
  39. Mavelli L, Ciriolo MR, Rotilio G, De Sole P, Castorino M, Stabile A. Superoxide dismutase, glutathione peroxidase and catalase in oxidative hemolysis. A study of Fanconi's anemia erythrocytes. Biochemical and Biophysical Research Communications. 1982;106(2):286-90. https://doi.org/10.1016/0006-291X(82)91107-X
  40. Kitani K, Minami C, Yamamoto T, Kanai S, Ivy GO, Carrillo MC. Pharmacological interventions in aging and age-associated disorders : potentials of propargylamines for human use. Annals of the New York Academy of Sciences. 2002;959(1):295-307. https://doi.org/10.1111/j.1749-6632.2002.tb02101.x