한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제30권4호
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  • Pages.49-61
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  • 2017
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
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익모초(益母草) 추출물이 악성 흑색종 세포에 미치는 피부미백효과

Skin Whitening Effect of Leonuri Herba Extracts(LHE) on Malignant Melanoma Cell

  • 김지은 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 임경민 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 나가영 (동신대학교 한의과대학 내과학교실) ;
  • 김혜화 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 강병수 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 최정화 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 박수연 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 정민영 (동신대학교 한의과대학 안이비인후피부과학교실)
  • Kim, Ji-Eun (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Lim, Kyeong-Min (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Na, Ga-yeong (Department of Internal medicine, College of Korean Medicine, Dong-shin University) ;
  • Kim, Hye-Hwa (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Kang, Byung-Soo (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Choi, Jung-Hwa (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Park, Soo-Yeon (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Jung, Min-Yeong (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University)
  • 투고 : 2017.10.16
  • 심사 : 2017.11.03
  • 발행 : 2017.11.25
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초록

Objectives : In order to find out the whitening effects of Leonuri Herba, this study was designed to identify the effects and the action mechanism of LHE(Leonuri Herba extract) on Malignant melanoma cell lines. Methods : After treating LHE on the B16F10 cell-Malignant melanoma cell line-, the cell survival rate, melanin biosynthesis rate, intra&extracellular tyrosinase activity rate, SOD-like activity, tyrosinase mRNA gene expression were investigated. The results were compared with control group without LHE treatment or with positive control group treated with whitening efficacy substance. Results : B16F10 cell survival rate, melanin biosynthesis rate, and intra&extracellular tyrosinase activity were significantly inhibited depending on the concentration of treated LHE. Melanin biosynthesis rate and tyrosinase activity rate were also decreased when ${\alpha}-MSH$ was combined with LHE. In addition, the SOD-like activity was increased in a concentration-dependent manner in the treatment with the LHE, indicating signigicant activity at high concentrations, and the tyrosinase mRNA gene expression was decreased in both the LHE-treated group, the LHE and ${\alpha}-MSH-treated$ group. Conclusions : LHE seems to inhibit melanin synthesis through inhibition of tyrosinase activity and inhibition of tyrosinase mRNA gene expression. It also has the effect of promoting SOD-like activity and may be used clinically as a skin whitening agent in the future.

키워드

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