• Title/Summary/Keyword: B.M.

Search Result 20,320, Processing Time 0.05 seconds

Comparative Studies on the Enzymatic Properties of Trypsins from Cat-shark and Mackerel -2. Enzymatic Properties of the Trypsins- (복상어와 고등어의 Trypsin에 관한 비교효소학적 연구 -2. 정제 Trypsin의 효소적 성질-)

  • PYEUN Jae-Hyeung;CHO Deuk-Moon;HEU Min-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.25 no.5
    • /
    • pp.383-391
    • /
    • 1992
  • A comparative study of enzymatic properties between the trypsin from the cat-shark Cephaloscyllium umbratile ( C-T) and the two trypsins from the mackerel Scomber japonicus $(M-T_A\;and\;M-T_B)$ was carried out following after the previous paper(Pyeun et al., 1991). Trypsin from cat-shark(C-T) showed the higher heat stability compared to the others $(M-T_A\;and\;M-T_B)$ and its denaturation constant$(K_D)$ was $10.68\times10^{-4}\;sec^{-1}\;at\;55^{\circ}C$ with BA-p-NA substrate. The activation energies(Ea) of the trypsins measured at a temperature range from $30^{\circ}C\;to\;50^{\circ}C$ were estimated to be 4.07 kcal/mole for C-T, 11.61 kcal/mole for $M-T_A$, and 8.43kcal/mole for $M-T_B$, respectively. The Km values were $24.9\times10^{-5}\;M\;for\;C-T,\;5.37\times10^{-5}\;M\;for\;M-T_A,\;and\;9.65\times10^{-5}\;M\;for\;M-T_B$. On the other hand, the Ki values for TLCK and DFP determined by Dixon plot were $1.50\times10^{-6}\;M\;and\;9.28\times10^{-6}\;M\;for\;C-T\;2.86\times10^{-6}\;M\;and\;2.11\times10^{-4}\;M\;for\;M-T_A\;and\;3.90\times10^{-6}\;M\;and\;1.60\times10^{-4}\;M\;for\;M-T_B$ Similar amino acid profiles were showed between three trypsins each other, with few exceptions of $M-T_B$ containing higher amount of arginine, and the smaller amount of tryptophan in C-T than the others.

  • PDF

Effect of Dexamethasone on Gene Expression of Surfactant Protein B and Surfactant Protein C (스테로이드제가 백서 폐의 Surfactant B와 C 유전자 발현에 미치는 영향)

  • Park, Ik Soo;Sohn, Jang Won;Yoon, Ho Joo;Shin, Dong Ho;Park, Sung Soo
    • Tuberculosis and Respiratory Diseases
    • /
    • v.54 no.4
    • /
    • pp.439-448
    • /
    • 2003
  • Background : Surfactant protein B(SP-B) and surfactant protein C(SP-C) are important in accelerating surface spreading of surfactant phospholipid. The glucocorticoids accelerate the morphologic differentiation of epithelial cells into type II cells and increase the rate of phosphatidylcholine synthesis. The hydrophobic surfactant protein has been shown to be upregulated by glucocorticoids in vitro, however, its regulation in vivo is not well established. Methods : The authors investigated the effects of glucocorticoid on the accumulation of mRNA encoding SP-B and SP-C protein content of the lung. Adult rats were given different doses of subcutaneous dexamethasone and sacrificed at 24 hours and 1 week. SP-B and SP-C mRNA were measured by a filter hybridization method. Results : 1) The accumulation of SP-B mRNA at 24 hours after 0.2 mg/kg dexamethasone treatment was increased by 23.7%. 2) The accumulation of SP-B mRNA at 1 week after 2 mg/kg dexamethasone treatment was significantly increased by 96.6%(P<0.001). 3) The accumulation of SP-C mRNA at 24 hours after 0.2 mg/kg dexamethasone treatment was significantly increased by 42.7%(P<0.01). 4) The accumulation of SP-C mRNA at 1 week after 2 mg/kg dexamethasone treatment was significantly increased by 60.0% (P<0.01). Conclusion : The authors concluded that dexamethasone treatment in vivo resulted in increased levels of SP-B mRNA and SP-C mRNA. These results suggested that dexamethasone stimulates the synthesis of hydrophobic proteins associated with surfactant.

E. M. Visualization and Electrophoresis analysis of B. thuringiensis var. kurstaki and B. thuringiensis var. israelensis $\sigma$-endotoxin (B. thuringiensis var. kurstaki와 B. thuringiensis var. israelensis 내독소 결정체의 전자현미경 관찰과 전기영동분석)

  • 이형환;강태숙;유관희
    • Microbiology and Biotechnology Letters
    • /
    • v.13 no.3
    • /
    • pp.315-319
    • /
    • 1985
  • Delta-endotoxin crystals of B. thuringiensis var. kurstari and B. thuringiensis var. israelensis were purified by NaBr density gradient centrifigation and the wet weight of the BTK endotoxin was approximately 23.79% of the cell wet weight and that of BTI was 25%. The shape of BTK crystal was bipyramidal, whose size was 1.7${\mu}{\textrm}{m}$ $\times$ 0.9${\mu}{\textrm}{m}$ and that of BTI was a spheroid, whose size was about 1.6$\times$0.45${\mu}{\textrm}{m}$. The molecular weight of BTK crystal protein was approximately 134,000 daltons and that of BTI was about 128,000 daltons.

  • PDF

Performance Analysis of Trellis Coded OFDM/M-ary PSK Systems in the presence of tarrier Frequency Offset (반송파 주파수 오프셋의 존재하에서 Trellis Coded OFDM/M-ary PSK 시스템의 성능 분석)

  • 조성언;박기식;오원근
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.5 no.4
    • /
    • pp.684-691
    • /
    • 2001
  • In this paper, the BER performance of OFDM/M-ary PSK systems is analyzed with considering carrier-frequency offset and TCM technique, which encodes and modulations simultaneously, is adopted to OFDWM-ary PSK systems for compensation of performance degradation according to carrier-frequency offset. As a result of analysis, the error performance of OFDM/M-ary PSK systems is degraded as the frequency offset is increased. And the frequency offset should be below 0.025 in order to achieve $BER= 10^{-3}$ with $E_b/N_o$ of 10 dB. Especially, when the TCM technique is adopted to OFDM/M-ary PSK systems, the performance improvement of about 4 dB is obtained in terms of $E_b/N_o$.

  • PDF

Composition of Vitamin A, E, $B_l$ and $B_2$ Contents in Korean Cow's Raw Milk in Korea (국내산 원유 중 비타민 A, E, $B_l$$B_2$ 함량에 관한 연구)

  • Kwak Byung-Man;Kim Sung-Han;Kim Kang-Seob;Lee Ki-Woong;Ahn Jang-Hyuk;Jang Chi-Hoon
    • Food Science of Animal Resources
    • /
    • v.26 no.2
    • /
    • pp.245-251
    • /
    • 2006
  • This study was performed to investigate the changes of vitamin A, vitamin E, vitamin $B_l$ and vitamin $B_2$ contents in cow's raw milk collected from dairy farms in Chungcheong-do and Jeolla-do for a year. The contents of fat soluble vitamin A and E were changed as seasonal effect, but water soluble vitamin $B_l$ and $B_2$ contents were not changed as seasonal effect. Vitamin A content in cow's raw milk was as follows [minimum ${\sim}$maximum (mean), ug/100 mL]; $35.1{\sim}59.0$ (44.4) in spring, $36.7{\sim}65.6$ (50.0) in summer, $28.7{\sim}61.2$ (46.8) in autumn and $29.9{\sim}57.8$ (43.1) in winter. In case of vitamin E was as follows [minimum${\sim}$maximum (mean), ug/100 mL]; $28.3{\sim}59.2$ (45.8) in spring, $39.6{\sim}69.9$ (58.8) in summer, $35.0{\sim}62.8$ (46.2) in autumn and $26.0{\sim}55.4$ (41.5) in winter. In case of vitamin $B_l$ was as follow [minimum${\sim}$maximum (mean), ug/100 mL]; $27.7{\sim}57.9$ (42.84) in spring, $32.4{\sim}66.1$ (49.39) in summer, $34.1{\sim}63.7$ (46.69) in autumn and $20.6{\sim}61.4$ (43.20 in winter. The amounts of vitamin $B_2$ in cow's raw milk was as follows [minimum${\sim}$maximum (mean), ug/100 mL]; $150{\sim}182$ (160 in spring, $145{\sim}185$ (163) in summer, $149{\sim}180$ (166) in autumn and $148{\sim}190$ (167) in winter.

DISCRETE SIMULTANEOUS ℓ1m-APPROXIMATION

  • RHEE, HYANG J.
    • Honam Mathematical Journal
    • /
    • v.27 no.1
    • /
    • pp.69-76
    • /
    • 2005
  • The aim of this work is to generalize $L_1$-approximation in order to apply them to a discrete approximation. In $L_1$-approximation, we use the norm given by $${\parallel}f{\parallel}_1={\int}{\mid}f{\mid}d{\mu}$$ where ${\mu}$ a non-atomic positive measure. In this paper, we go to the other extreme and consider measure ${\mu}$ which is purely atomic. In fact we shall assume that ${\mu}$ has exactly m atoms. For any ${\ell}$-tuple $b^1,\;{\cdots},\;b^{\ell}{\in}{\mathbb{R}}^m$, we defined the ${\ell}^m_1{w}$-norn, and consider $s^*{\in}S$ such that, for any $b^1,\;{\cdots},\;b^{\ell}{\in}{\mathbb{R}}^m$, $$\array{min&max\\{s{\in}S}&{1{\leq}i{\leq}{\ell}}}\;{\parallel}b^i-s{\parallel}_w$$, where S is a n-dimensional subspace of ${\mathbb{R}}^m$. The $s^*$ is called the Chebyshev center or a discrete simultaneous ${\ell}^m_1$-approximation from the finite dimensional subspace.

  • PDF

Metabolism of Soyasaponin I by Human Intestinal Microflora and Its Estrogenic and Cytotoxic Effects

  • Chang, Seo-Young;Han, Myung-Joo;Han, Sang-Jun;Kim, Dong-Hyun
    • Biomolecules & Therapeutics
    • /
    • v.17 no.4
    • /
    • pp.430-437
    • /
    • 2009
  • Metabolites of Soyasaponin I, a major constituent of soybean, by human intestinal microflora were investigated by LC-MS/MS analysis. We found four peaks, one parental constituent and three metabolites: m/z 941 [M-H]$^-$, m/z 795 [M-rha-H]$^-$, m/z 441 [aglycone-$H_2O$+H]$^+$, and m/z 633 [M-rha-gal-H]$^-$, which was an unknown metabolite, soyasapogenol B 3-$\beta$-D-glucuronide. When soyasaponin I was incubated with the human fecal microbial fraction from ten individuals for 48 h, soyasaponin I was metabolized to soyasapogenol B via soyasaponin III and soyasapogenol B 3-$\beta$-D-glucuronide or via soyasaponin III alone. Both soyasaponin I and its metabolite soyasapgenol B exhibited estrogenic activity. Soyasaponin I increased the proliferation, mRNA expression of c-fos and pS2, in MCF7 cells more potently than soyasapogenol B. However, soyasapogenol B showed potent cytotoxicity against A549, MCF7, HeLa and HepG2 cells, while soyasaponin I did not. The cytotoxicity of soyasapogenol B may prevent its estrogenic effect from increasing dose-dependently. These findings suggest that orally administered soyasaponin I may be metabolized to soyasapogenol B by intestinal microflora and that soyasapogenol B may express a cytotoxic effect rather than an estrogenic effect.

Some Topologies Induced by b-open Sets

  • El-Monsef, M.E. Abd;El-Atik, A.A.;El-Sharkasy, M.M.
    • Kyungpook Mathematical Journal
    • /
    • v.45 no.4
    • /
    • pp.539-547
    • /
    • 2005
  • The class of b-open sets in the sense of $Andrijevi{\acute{c}}$ ([3]), was discussed by El-Atik ([9]) under the name of ${\gamma}-open$ sets. This class is closed under arbitrary union. The aim of this paper is to use ${\Lambda}-sets$ and ${\vee}-sets$ due to Maki ([15]) some topologies are constructed with the concept of b-open sets. $b-{\Lambda}-sets,\;b-{\vee}-sets$ are the basic concepts introduced and investigated. Moreover, several types of near continuous function based on $b-{\Lambda}-sets,\;b-{\vee}-sets$ are constructed and studied.

  • PDF

The Spinnability of Ag/PET Master Batch containing Silver Nano Particles according to Changing of Intrinsic Viscosity (은나노입자 함유 M/B의 고유점도(IV)변화에 따른 제사성 및 가연성에 관한 연구)

  • Son, Eun-Jong;Kim, Hyun-Sun;Choi, Tae-Soo;Jeong, Sung-Hoon
    • Proceedings of the Korean Society of Dyers and Finishers Conference
    • /
    • 2011.03a
    • /
    • pp.54-54
    • /
    • 2011
  • 방사 공정 최적화를 위해 칩(chip)건조를 실시하여 칩의 수분을 모두 제거한 후 실험을 진행하게 된다. 칩은 그 자체가 공정수분율(0.4%) 또는 그 이상의 수분을 함유하고 있으므로 건조하지 않고 방사하면 현저히 가수분해가 일어난다. 가수분해가 일어난다면 PET 분자량도 저하되어 고분자의 성질을 잃게 되어 방사된 섬유의 물리 화학적 성질에 중요한 영향을 받게 된다. 그러므로 가수분해를 방지하기 위하여 칩내 수분을 제거하는 건조 공정을 거치는 것이다. 개발된 나노은입자을 함유한 Ag/PET 마스터배치의 제사성 및 가연성 평가을 위한 파일럿연구를 행했다. 본 연구에서 사용한 은나노 M/B 칩(chip)의 경우 일반적으로 사용하는 PET 칩에 비하여 낮은 고유점도를 가지므로 방사성에 칩의 수분이 더욱 영향을 미칠 것이라 판단되어 건조공정에 특별한 주의을 하여 진행하였다. 마스터배치의 고유점도(IV)값의 변화에 따른 제사성 및 가연성 평가를 관찰하였다. #3 M/B 칩의 제사성이 #1 M/B 칩 대비 공정성이 개선되어 두 품종 모두 비출사가 발생하지 않았으며, M/B 제조시 분산제 유무에 따른 방사공정성의 차이는 없는 것으로 보인다. 따라서 #1 M/B 대비 공정성이 개선된 점은 M/B의 IV개선에 기인하는 것으로 판단되었다.

  • PDF

A Study on the sound characteristic and B.M.I by Sasang Constitution (사상체질별 음향특성(音響特性)과 신체질량지수(BMI)에 관(關)한 연구(硏究))

  • Kim, Dal-Rae
    • Journal of Sasang Constitutional Medicine
    • /
    • v.16 no.1
    • /
    • pp.53-60
    • /
    • 2004
  • Purpose This study is to find the characteristics of voice quality based on the classifying the sound characteristics and B.M.I. by Sasang Constitution. Methods To make the notion of the consensus of Sasang Constitution's Voice, classification into 4 categories was made: clear/hoarse, high/low, powerful/powerless, fast/slow. Result The voice quality of Soyangin group was classified as powerful and fast, and that of Taeumin group was classified as powerful and hoarse and low, and that of Soeumin group was classified as powerless and clear. The mean B.M.I. of Soeumin group was classified as 21.4, and that of Taeumin group was classified as 26.3. Conclusion 1. Taeumin was significantly high compared with Soeumin in B.M.I. 2. It can be classified as Taeumin when B.M.I. is high(26.3). 3. It can be classified as Soeumin when B.M.I. is low(21.4). 4. The voice quality of Soyangin group was classified as clear and fast, or strong and clear, and that of Taeumin group as powerful and hoarse, and that of Soeumin group as powerless and low.

  • PDF