• 한국식품영양과학회지
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• 제30권2호
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• pp.222-227
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• 2001

The members of the genus Vibrio include harmless aquatic strain as well as strains capable of causing infections in human and fish. Pathogenic mechanisms are only understood for Vibrio cholerae O1 and O139 and not for the majority of Vibrio species. Twelve clinical and nonclinical strains were examined by in vitro and in vivo experiments for the importance of extracellular enzymes as a virulence determinant of Vibrio species. In vivo cytotoxicity assay was performed by injecting approximately $10^{8}$ cells/mL into mice (BALB/c). V. harvyi and V. vulnificus showed 100% lethality within 3hr after bacterial injection. V. fluvialis and four strains of V. parahaemolyticus showed 50% lethality within 4hr. V. mimicus, V. alginolyticus and V. furnissii revealed 30% lethality within 9hr. Nonclinical strains, V. campbellii and V. ordalii, did not show any lethality. In vitro protease and hemolytic activities were also good indicators for clinical and nonclinical strains of Vibrio species. The clinical strains showed much higher activities than nonclinical strains. The activity of some clinical strains of re-isolates was evidently increased. Most clinical strains had $\beta$ hemolytic activity. The results demonstrate that the prevalent distribution of extracellular proteases in pathogenic Vibrio sp. implies their importance as a virulence determinant.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 하계학술대회 논문집 A
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• pp.208-211
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• 2003

The western route of KEPCO's 765 kV transmission line has been tentatively operating as 345 kV voltage before commercial operation. KEPCO decided to operate the 765 kV line for commercial operation after completing the test operation of 765 kV substation in 2002. In the process of energizing the line as 765 kV voltage, double circuit transmission line will be operated with two voltage grades of 765 kV and 345 kV. As the earthing switches are installed on both ends of the line, electrostatic induction voltage and electromagnetic induction current were calculated prior to the line energizing in order to confirm the ratings. The induced voltage and current are important for the maintenance of the parallel circuit. This paper presents the simulation results of electrical phenomena such as electrostatic induction voltage and electromagnetic induction current from the parallel line. The transmission line was modeled by EMTP (Electro-Magnetic Transient Program). The simulation results were compared with the measured results at the field.

• Journal of Information Display
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• 제7권3호
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• pp.27-30
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• 2006

This paper presents a comprehensive study on threshold voltage $(V_{th})$ control of organic thin-film transistors (OTFTs) with dual-gate structure. The fabrication of dual-gate pentacene OTFTs using plasma-enhanced atomic layer deposited (PEALD) 150 nm thick $Al_{2}O_{3}$ as a bottom gate dielectric and 300 nm thick parylene or PEALD 200 nm thick $Al_{2}O_{3}$ as both a top gate dielectric and a passivation layer was investigated. The $V_{th}$ of OTFT with 300 nm thick parylene as a top gate dielectric was changed from 4.7 V to 1.3 V and that with PEALD 200 nm thick $Al_{2}O_{3}$ as a top gate dielectric was changed from 1.95 V to -9.8 V when the voltage bias of top gate electrode was changed from -10 V to 10 V. The change of $V_{th}$ of OTFT with dual-gate structure was successfully investigated by an analysis of electrostatic potential.

• Corrosion Science and Technology
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• 제21권4호
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• pp.290-299
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• 2022

Anodizing is an electrochemical surface treatment method conferring corrosion resistance and durability by forming a thick anodization film on the metal surface. Aluminum has a long service life and high thermal conductivity and formability, as well as excellent corrosion resistance. Aluminum 3003 alloy has improved formability, strength, and corrosion resistance due to the addition of a small amount of manganese. However, corrosion occurs in seawater and environments polluted with corrosion-inducing substances, which reduce corrosion resistance. Therefore, it is necessary to artificially form a thick anodized film to improve corrosion resistance. In this study, the anodization treatment time was 4 minutes, and voltages of 10 V, 20 V, 30 V, 40 V, 50 V, 60 V, 70 V, 80 V, 90 V, and 100 V were applied. The thickness and pore size of the oxide film increased according to the applied voltage. A barrier film was formed under voltage conditions from 10 V to 50 V, and a porous film was formed under voltage conditions from 60 V to 100 V. After anodizing, coating was applied. Wettability and corrosion resistance were observed before and after coating according to the surface shape and thickness of the oxide film.

• Journal of Microbiology and Biotechnology
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• 제22권8호
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• pp.1107-1112
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• 2012

Outbreaks of foodborne diseases associated with Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae frequently occur in countries having a dietary habit of raw seafood consumption. For rapid identification of different Vibrio species involved in foodborne diseases, whole-cell protein pattern analysis for 13 type strains of 12 Vibrio species was performed using SDS-PAGE analysis. Pathogenic Vibrio species such as V. parahaemolyticus, V. vulnificus, V. cholerae, V. alginolyticus, V. fluvialis, and V. mimicus were included in the 12 Vibrio species used in this study. Each of the 12 Vibrio species showed clearly specific band patterns of its own. Two different strains of V. parahaemolyticus showed two different SDS-PAGE whole-cell protein patterns, giving the possibility of categorizing isolated strains in the same V. parahaemolyticus species into two subgroups. The 36 Vibrio isolates collected from sushi restaurants in Busan were all identified as V. parahaemolyticus by comparing their protein patterns with those of Vibrio type strains. The identified isolates were categorized into two different subgroups of V. parahaemolyticus. The whole-cell protein pattern analysis by SDS-PAGE can be used as a specific, rapid, and simple identification method for Vibrio spp. involved in foodborne diseases at the subspecies level.

• Biomolecules & Therapeutics
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• 제31권2호
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• pp.168-175
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• 2023

Tramadol is an opioid analog used to treat chronic and acute pain. Intradermal injections of tramadol at hundreds of millimoles have been shown to produce a local anesthetic effect. We used the whole-cell patch-clamp technique in this study to investigate whether tramadol blocks the sodium current in HEK293 cells, which stably express the pain threshold sodium channel Na_v1.7 or the cardiac sodium channel Na_v1.5. The half-maximal inhibitory concentration of tramadol was 0.73 mM for Na_v1.7 and 0.43 mM for Na_v1.5 at a holding potential of -100 mV. The blocking effects of tramadol were completely reversible. Tramadol shifted the steady-state inactivation curves of Na_v1.7 and Na_v1.5 toward hyperpolarization. Tramadol also slowed the recovery rate from the inactivation of Na_v1.7 and Na_v1.5 and induced stronger use-dependent inhibition. Because the mean plasma concentration of tramadol upon oral administration is lower than its mean blocking concentration of sodium channels in this study, it is unlikely that tramadol in plasma will have an analgesic effect by blocking Na_v1.7 or show cardiotoxicity by blocking Na_v1.5. However, tramadol could act as a local anesthetic when used at a concentration of several hundred millimoles by intradermal injection and as an antiarrhythmic when injected intravenously at a similar dose, as does lidocaine.

• Korean Chemical Engineering Research
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• 제51권3호
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• pp.313-318
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• 2013

$V_2O_5-WO_3/TiO_2$ 촉매의 질소산화물 환원반응에 있어서 $V_2O_5$ 함량이 NO 환원 및 $N_2O$ 생성에 미치는 영향을 조사하기 위하여 분말촉매를 사용한 미분반응기에서 실험 연구를 수행하였다. 고정된 비율의 $WO_3$와 $TiO_2$에 $V_2O_5$ 함량을 1에서 8 wt%까지 변화시킨 촉매에서 NO 환원반응과 암모니아 산화반응 특성이 조사되었다. $V_2O_5-WO_3/TiO_2$ 촉매에서 NO 환원 반응은 $200^{\circ}C$ 이하에서도 상당량 진행되지만, $V_2O_5$ 함량을 1 wt% 촉매의 경우 700 ppm의 NO를 99.9%이상 전환시키는 최저 반응온도가 $340^{\circ}C$에서 아주 좁은 활성 온도창으로 일어났다. 그리고 이 활성온도는 촉매의 $V_2O_5$ 함량이 증가됨에 따라 점점 저온 쪽으로 이동하여, 6 wt% 촉매의 경우 $220{\sim}340^{\circ}C$에서 높은 활성을 보였다. $V_2O_5$ 함량이 8 wt% 촉매의 경우 전 온도 구간에서 6 wt% 촉매보다 낮은 NO 환원율을 보였다. 그러나 반응온도 $340^{\circ}C$ 이상에서는 촉매의 $V_2O_5$ 함량이 증가함에 따라 NO 전환율이 감소하였다. 이는 $V_2O_5-WO_3/TiO_2$ 촉매의 NO 환원을 위한 촉매 활성점 상당 크기 이상의 $V_2O_5$ 입자와 관계되는 것으로 판단되며 촉매 입자가 클수록 $320^{\circ}C$ 이상에서 암모니아 산화에 의해 발생되는 $N_2O$ 생성을 고려하여야 한다. $V_2O_5-WO_3/TiO_2$ 촉매는 배기가스 중의 질소산화물 제거를 위하여 현재 통상적으로 $350{\sim}450^{\circ}C$의 영역에서 운전되고 있으나, 고온 영역에선 2차 오염물인 $N_2O$의 발생을 피할 수 없고 에너지 소비량이 많으므로, $250{\sim}320^{\circ}C$의 저온 영역에서 적합한 촉매로써 $V_2O_5$ 함량이 높은 $V_2O_5-WO_3/TiO_2$ 촉매의 사용이 권장되었다.

• 자원환경지질
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• 제38권5호
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• pp.525-534
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• 2005

지질매체에서 흔하게 발견되는 호기성 박테리아인 Pseudomonas aeuginosa에 대하여 영양분을 공급하지 않은 상태에서 As(V) 및 As(III) 흡착실험을 수행하였다. As(111)의 경우 P. aeruginosa에 의한 소규모의 흡착이 관찰된 반면, As(V)는 효과적인 흡착이 적용되지 않음을 확인할 수 있었다 이는 As(V)는 수용액 상태에서 음전하 화합물로 존재하고 박테리아 표면 역시 음전하를 띠고 있기 때문에 상호 인력이 작용하지 않기 때문인 것으로 파악된다. 그러나 바이오필름 상태의 박테리아는 많은 양의 용존 As(V)를 As(III)로 환원하는 것으로 나타났다. 이는 빈영양 환경인 대부분의 지질환경에서도 미생물이 용존 비소의 거동에 미치는 영향이 크다는 것을 의미한다. 다양한 중금속으로 오염된 지하수를 처리하기 위하여 구축된 미생물 반응벽체는 비소의 흡착을 촉진하기보다는 오히려 비소의 독성과 이동도를 증가시키는 부의 효과를 유도할 수도 있다.

• 한국식품저장유통학회지
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• 제2권2호
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• pp.269-276
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• 1995

Uncooked rice Tackju was prepared from the groud rice with different particle size such as 12, 20, 35 and 50 mesh, and its characteristics were analysed during the brewing. Reducing sugar and ethanol contents as well as yeast cell counts were the highest in main mash and Tackju prepared from 20 mesh fraction. Especially, reducing sugar content was about. 1.5-2.5 times as much as that obtained from 35 or 50 mesh fraction. The final concentration of ethanol in main mash prepared from 20 mesh fraction was 14.8%(v/v). It was about 1.5%(v/v) higher than the lowest one [13.35%(v/v)] which was prepared from 12 mesh fraction. No significant difference In total acidity was observed. Sensory evaluation revealed 20 or 35 mesh Tackju to be the best in overall preference, especially in sweet and refreshing tastes.

• 미생물학회지
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• 제29권5호
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• pp.319-328
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• 1991

this study was performed for the purpose of surveying the distribution and characteristics of the Vibrio species in the diseased farm flounders (Paralichthys olivaceus) during the winter season in Korea. Samples were collected along the southern coast in the Sinam, Keoje Island, and Teosu areas from November '90 to Feburary '91. Nine species of Vibrio were identified as V. tubiashii (85 strains), V. damsela (78 strains), V. anguillarum (21 strains), V. compbelli (9 strains), V. fluvialis (9 strain), V. costicola (1 strain), V. alginolyticus (1 strain), V. gazogenes (1 strain), V. marinus (1 strain), and unidentified 3 groups of bacteria (154 strains) which may have related with V. tubiashii were isolated. Eleven kinds of discs (BBL Co.) were used for antibiotic susceptibility test and 116 strains were selected for the experiment. Most bacteria were powerfully inhibited their growth by 4 antibiotics such as cephalothin, chloramphenicol, colistin, and tetracycline, but they were poorly inhibited by 4 antibiotics such as carbenicillin, kanamycin, penicillin G, and streptomycin.