• Journal of Pharmacopuncture
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• v.13 no.3
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• pp.47-62
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• 2010

Objectives: Colitis is an inflammatory bowel disease characterized by colonic mucosal inflammation and chronic relapsing events represents. The purpose of this study is to evaluate effects of pharmacopuncture of anti-inflammatory herbal compound (AiC) applied to the different acupoints in the acute colitis induced by trinitrobenzenesulphonic acid (TNBS) intracolonic injection in rats. Methods: In Male Sprague - Dawley rats, weighing 250~400g, TNBS (5 mg/kg) was infused intrarectally through a silicon rubber catheter into the anus under isoflurane anaesthesia. Acupoints of LI4 (Hapkok), ST25 (Cheonchu), ST36 (Joksamni), and BL25 (Daejangsu) were intramuscularly injected by AiC, respectively (injection volume & times: 0.2 ml / acupoint, twice times on the 2nd & 3rd day). Expressions of cFos protein in the periaqueductal gray (PAG), locus coeruleus (LC), nucleus of solitary tract (Sol), and the 6th lumbar spinal cord (L6 s.c.) were observed at 24 hr after TNBS induced colitis by immunohistochemistry. Results: The expression of c-Fos protein in the L6 s.c., Sol, LC and PAG increased 24 hr after TNBS injection into colorectum as compared to normal and 50% ethanol treated group. AiC to LI4 inhibited the expression of c-Fos protein in Sol and PAG but not L6 s.c. and LC. AiC to ST36 showed significant inhibition the c-Fos expression in L6 s.c., Sol and PAG. AiC to ST25 only showed the effects in L6 s.c. and PAG. AiC to BL25 inhibited significantly the expression of c-Fos protein all over the areas. To investigate whether or not endogenous opioids are involved, intrathecal injection of naltrexone (30ug/30ul) was applied before the 2nd pharmacopuncture treatment 24 hr after TNBS-induced colitis in rat. Naltrexone reversed the inhibition of c-Fos protein expression in the spinal cord and brainstem. Conclusions: These data show that pharmacopuncture of Aic potently inhibits signal pathways ascending hypersensitivity of colorectum after TNBS induced colitis and depends on the endogenous opioids according to acupoints.

• The Korean Journal of Pesticide Science
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• v.12 no.3
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• pp.291-294
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• 2008

The Streptomyces padanus isolate TH04, isolated from mummified peaches, showed strong antifungal activity to Monilinia fructicola. The inhibition activity of the isolate TH04 to mycelial growth and spore germination at 1% concentration of sub-antifungal powder made from culture suspension (CS) was ranged from 79.8% to 81.0% and from 73.9% to 75.8% to M. fructicola four strains, respectively. In the test of antifungal activity in mixed culture of the isolate and M. fructicola, inhibition rate was 7.5%, 86.8% and 94.0% in 0.01, 0.1, and 1% concentration of CS containing bacterial cell of the isolate, respectively. On apples (cultivar; Fuji), the control values of the isolate TH04 crude filtrates (0.1 and 1%) were 85.9% and 100%, respectively. The results suggest that the isolate TH04 indicate development possibility as biocontrol agent of brown rot caused by M. fructicola with the study on delivery method and fermentation condition to produce an antifungal compound.

• Korean Journal of Ichthyology
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• v.12 no.3
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• pp.208-214
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• 2000

The egg development and morphological changes of larvae of the file fish, Thamnaconus modestus(Gunther), was investigated by artificial insemination. The fertilized egg was spherical in shape, colorless and adhesive with 0.63~0.67mm in diameter and yolk had 20~30 oil globules of various size (0.04~0.16 mm in diameter). The hatching occured at 53 hours after fertilization in $21.0\sim23.0^{\circ}C$ of water temperature. Hatched larvae measured 2.07~2.20 mm in total length with 5~6+17~18=22~24 myotomes. The 3~4 days after hatching larvae grew to the total length 2.62~2.77 mm, opened mouth and anus. The 5 days after hatching, total length of the larvae was 2.74~3.04 mm, the first dorsal fin was formed at the behind of head. After hatching 12~13 days larvae attained 3.78~3.99 mm in total length. This period formed rudimental dorsal fin and anal fin. Twenty days after hatching, larvae grew to 6.04~6.17mm in total length, at which time the dorsal, anal and caudal fin rays reached 19~20, 18~19 and 9~10, respectively.

• Journal of Aquaculture
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• v.11 no.1
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• pp.49-58
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• 1998

The spawning behavior and early life history of the Korean aucha perch, Coreoperca herzi were studied. The eggs were spawned in a one-layer mass, hanging from the underside of a largy stone and guarded by one male. According to the considerable size difference os eggs in the individual ovaries, it was concluded that they spawns several times within a spawning season of May~June. The ripe eggs were demersal and adhesive, and their diameter were 2.60~3.04mm with several oil globule of 0.10~0.64mm. Hatching in the tank with $19.0{\pm}1.0^{\circ}C.$ in men water temperature started from the 380 hours after fertilization. Newly-hatched larvae were measured 6.45~7.34mm in total length (TL, mean : 6.85mm), and mouth and anus were open. Melanophores were present on the head, around the yolk, and on the dorsal part of the abdominal region. 3 days old larvae were measured 7.00~7.40mm in TL (mean : 7.25mm), and they fed Daphnia sp. and Brachionus plicatilis actively. 6 days old larvae transformed to postlarval stage and they were 7.94~10.10mm in TL (mean : 9.00mm). As yolk sac were nearly absorbed, the caudal notochord flex at 45. Aggregate numbers of all fin rays were completed at over 16.90~19.80mm in TL (30 days after hatching), at which time the larvae reached the juvenile stage. In fifty days old larvae(24.60~27.10mm in TL) were similar in body form, lateral line and color to adult.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.4
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• pp.323-328
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• 1982

Kareius bicoloratus (Basilewsky) is one of the commonly found right-eye flounders and widely distributed in the coastal waters of Korea and Japan. On December 11,1980, the ailthors carried out an experiment to obtain a large number of fertilized eggs from wild adult fish caught by a trawler. The fish were obtained from Maisaka fish market, Shizuoka Prefecture, Japan. The egg is pelagic, spherical in shape and measuring 1.014-1.04 mm in diameter. The yolk as well as the egg capsule is colorless and transparent, and contain no oil globules. The hatching took place in 73 hr 45 min after fertilization at the water temperature $8.0-9.5^{\circ}C$. Newly hatched larvae are 3.09-3.146 mm in total length, with the anus situated in the middle of the body. The marginal fin does not have Pigment cells and myotome number is 17+20=37. Within one day after hatching, the larvae attained 3.77 mm in total length, and there appeared three or four melanophore on yolk sac. When the larvae attained 3.96 mm in total length, melanophores began to appear on the eye ball. Two days after hatching, the larvae attained 4.05 mm in total length, most of yolk material was absorbed, and the caudal fin began to grow at the terminal part of the notochord. When the larvae attained 4.21 mm in total length, mouth and eyes began to move. After 3 days, the larvae attained 4.342-4.394 mm in total length, alimentary canal differentiated, melanophores appeared on the lower jaw and posterior part of the fin membrane. When the larvae attaind 4.576 mm in total length, marginal line of dorsal fin membrane became concave.

• Korean Journal of Ichthyology
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• v.10 no.1
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• pp.115-127
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• 1998

The digestive tract of the rockfish, Sebastes schlegeli composed of pharynx, esophagus, stomach, intestine, anus and ten or eleven pyloric caeca. Pyloric caeca is blind sac of banana shape, and that is originated from pyloric portion of the stomach. The relative length of gut(RLG), that is length of digestive tract to standard length, is about 1.56(n=10). Esophageal muscularis consists of thin outer layer of longitudinal muscle and thick inner layer of circular muscle. Mucosal epithelium consists of columnar epithelium with short microvilli and contains numerous mucous secretory cell. The mucosal folds of the stomach are regular, and the muscularis consists of longitudinal, oblique and circular muscle layer. The chief cell of the gastric gland have a tubular mitochondria, endoplasmic reticula and numerous secretory granules in electron-dense. However, parietal cell contains small mitochondria, endoplasmic reticula and vacuoles in low electron density. Mucosal epithelium of the pyloric caeca and intestine composed of columnar epithelium, goblet cell, rodlet cell and dark cell. Columnar absorptive cell in the pyloric caeca and intestine contains well developed mitochondria, endoplasmic reticula, vesiculated granules in high electron density, pinocytotic vesicles and multivesicular body. Rodlet cell have a well developed cytoplasmic capsule and the endoplasmic reticula in the cytoplasm. Dark cell showing a high electron density in the cytoplasm and contains well developed mitochondria. Columnar epithelium of the intestine have a well developed intercellular junction and the microvilli which contains actin filament originated from the cytoplasm. Mucosal epithelium of the intestine have a longer microvilli and more abundant goblet cells than in the pyloric caeca.

• Korean Journal of Ichthyology
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• v.29 no.1
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• pp.52-61
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• 2017

The egg development and early life history of Korean endemic fish, Squalidus multimaculatus (Gobioninae), were investigated. The eggs from the females were obtained by injecting 10 IU/g of human chorionic gonadotropin and inseminated by wet method in the laboratory. The fertilized eggs were 0.8~0.9 mm in diameter and had no oil globules. The embryo began to hatch about 65 hrs after fertilization under water temperature of $24{\pm}1^{\circ}C$. The newly-hatched larvae were 2.5~3.1 mm in total length, and their mouth and anus were not opened. Four days after hatching, the postlarva were 4.0~4.2 mm in total length, and their york sacs were completely absorbed. They entered the juvenile stage when all fin-rays were formed at 30 days after hatching, and their total length were 11.2~15.7 mm. At 45 days after hatching, the external from of juveniles were similar to those of adults (total length were 18.8~22.5 mm), and 80 days after hatching, the external characteristics from of juveniles were same to adults (total length were 25.7~35.9 mm).

• Korean journal of applied entomology
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• v.51 no.2
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• pp.141-148
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• 2012

An outbreak of Ricania sp. occurred in the Kurye, Jeonnam area in 2011. This outbreak damaged many kinds of fruit trees such as Cornus, Persimmon and Chestnut. This experiment was conducted to survey the occurrence ecology of Ricania sp. such as host plants, oviposition characters, morphological characters and life cycle, as well as to select environmental friendly control agents. Ricaina sp. host plants included 51 species such as 32 xylophytes, and 19 herbaceous plants. Ricaina sp. preferred Cornus officinalis, Diospyros kaki, Castanea crenata, Eucommia ulmoides, Styrax japonicus for oviposition. Adults laid eggs on new inner twigs with 28.8 eggs per egg-mass. Egg size was 1.24 mm(length), 0.55 mm(width) in an oval shape. Nymphs molted four times. Every nymph stage had an x shape of yellow or white beeswax around the anus. Overwintered eggs of Ricania sp. hatched from the mid May to early June. Nymphal periods were from mid May to mid August and adults appeared from mid July but spawning began in mid August. Ricania sp. damaged new twigs by oviposition and retarded growth by sucking nutrients and producing a sooty mold. Sophora and natural plant extracts were effective environmentally friendly agricultural materials used to control the nymph and adult Ricania sp. Mortality was > 80%.

• Food Science and Preservation
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• v.12 no.2
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• pp.190-194
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• 2005

This study investigated the characteristics of honey wine, which was fermented by Saccaromyces sake, Saccharomyces bayanus and Nuruk. For alcohol fermentation of the diluting solution, mixtures of S. sake and Nuruk, and Nuruk and S. bayanus resulted the excellent fermentation. These conditions also produced a higher alcohol content than that from the single yeast Strain. The values of pH and acidity of honey wine showed little changes during the fermentation processes. In the case of the mixture of Nuruk and yeast, the content of reducing sugar during the fermentation processes decreased rapidly, but the content of alcohol increased. The soluble solids were $7.5\~8.1\;^{\circ}Brix$ after the fermentation period of 6 days, and the alcohol contents were represented $12.5\~13.1\%$, from the mixture of Nuruk and yeast. In addition, the sensory evaluation of honey wine with the mixture of Nuruk and yeast showed more effective results than that of the single yeast bacillus.

• Development and Reproduction
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• v.20 no.2
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• pp.109-118
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• 2016

This study monitored the morphological development of embryo, larvae and juvenile yellowtail kingfish, Seriola lalandi, for their aquaculture. The fertilized eggs obtained by natural spawning were spherical shape and buoyant. Fertilized eggs were transparent and had one oil globule in the yolk, with an egg diameter of $1.35{\pm}0.04mm$ and an oil globule diameter of $0.32{\pm}0.02mm$. The fertilized eggs hatched 67-75 h after fertilization in water at $20{\pm}0.5^{\circ}C$. The total length (TL) of the hatched larvae was $3.62{\pm}0.16mm$. During hatching, the larvae, with their mouth and anus not yet opened. The yolk was completely absorbed 3 days after hatching (DAH), while the TL of post-larvae was $4.72{\pm}0.07mm$. At 40 DAH, the juveniles had grown to $30.44{\pm}4.07mm$ in TL, body depth increased, the body color changed to a black, yellow, and light gray-blue color, and 3-4 vertical stripes appeared. At 45 DAH, the juveniles were $38.67{\pm}5.65mm$ in TL and $10.10{\pm}0.94mm$ in body depth. The fish were green with a light orange color, with 7 faint green-brown stripes on the sides of their body. At 87 DAH, the juveniles had grown to 236.11 mm in TL, 217.68 mm in fork length, and 136.5 g in weight. The fish resembled their adult form, with a light yellow-green body color, loss of the pattern on the sides of their body, and a yellow coloration at the tip of the caudal fin.