• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1333-1337
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• 2004

Oxidants such as hydrogen peroxide are powerful inducers of cell damage, ageing, and apoptosis. Since clusterin, a 75-80 kDa mammalian glycoprotein, is frequently found to be inducible in apoptotic cells and tissues, this study inquired into whether this would be a protective mechanism against further cell death. The aim was to find out whether overexpression of clusterin could protect cells from oxidant­induced stress and apoptosis. To clarify this issue, we generated and analyzed stable cell lines expressing fusion proteins of a rat clusterin with an enhanced green fluorescent protein (EGFP). When treated with varying concentrations of hydrogen peroxides, clusterin transfectants indeed showed increased resistance to apoptosis and exhibited a much higher survival rate than mock-transfected cells. On the other hand, neither intracellular re-distribution nor local concentration of clusterin-EGFP was observed, which leaves the question open about its anti-apoptotic mechanism. In conclusion, the overexpression of clusterin provides a means for protecting cells against oxidative stress and subsequent cell death.

• 약학회지
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• 제53권1호
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• pp.1-5
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• 2009

This study was performed to investigate the effects of acteoside and isoacteoside isolated from Clerodendron trichotomum Thunberg on melanin production in B16 melanoma cells. In DPPH radical scavenging activity, acteoside and isoacteoside had a potent anti-oxidant activity in a dose-dependent manner. Both acteoside and isoacteoside dose-dependently inhibited silica-induced ROS (reactive oxygen species) generation in B16 melanoma cells. They significantly inhibited tyrosinase activity and melanin production in MSH-stimulated B16 melanoma cells. The inhibitory effect of acteoside was more potent than that of isoacteosidee. In Western blot of tyrosinase, acteoside inhibited MSH-induced tyrosinase expression in B16 melanoma cells, which is related to the inhibitory action of acteoside on tyrosinase activity and melanin production. These results show that acteoside and isoacteoside from Clerodendron trichotomum Thunberg has a potent antioxidant activity and whitening activity. The underlying mechanism of acteoside on whitening activity may be due to the inhibition of tyrosinase activity and tyrosinase expression.

• 대한한방내과학회지
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• 제29권3호
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• pp.752-764
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• 2008

Objectives : This study was carried out to investigate the effects of Doche-tang on colonic mucosal ulcer induced by dextran sulfate sodium(DSS). Method : The group was divided into three. The normal group consisted of mice that were not inflammation-induced. The control group was composed of untreated colitis elicited mice. The sample group was administered Doche-tang after colitis elicitation. The effects on colonic mucosal ulcers were evaluated by the morphological change of colonic mucosa, the anti-oxidant effect, HSP 70, $NF-{\kappa}B$, COX-1, COX-2 and iNOS. Results : In terms of immunohistochemical findings, the distribution of COX-1 in mice treated with Doche-tang noticeably increased more than that in the control group. The distribution of HSP70, $NF-{\kappa}B$, COX-2, iNOS in mioe treated with Doche-tang decreased more than that in the control group. Regeneration of surface epithelial cell and goblet cell in mucosa was observed by transmission electron microscope. Conclusion : According to the results, Doche-tang is practicable treatment for colonic mucosal ulcer.

• 동의생리병리학회지
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• 제24권4호
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• pp.559-565
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• 2010

Cancer is already a well-recognized main cause of mortality and the incidence of cancer is increasing steadily. Because conventional treatment modalities for cancer accompanies severe side effects, traditional medicine has been considered as alternatives to reduce the adverse effects and its use has continued to rise in cancer therapy. This study aims to summarize and make a reference of radioprotective effects of herbs worldwide. In this process, this review surveyed all papers of radioprotective-focused studies using herbal medicine in PubMed database and finally 44 papers were included. The type of materials, formation of experiments, type of herbal medicine, their action and mechanisms, and type of cancer were analyzed. The number of studies on radioprotective effects of herbal medicine has increased since 2000. The main formation of experiments was clinical study and the portion was 45% and the proportion of the research using prescriptions was 51% and the research using herbal products was 25%. Herbs and prescriptions having the effects of tonifying and nourishment were used the most. Most of herbal medicine in this study can enhance immune function, increase anti-oxidant effect, regulate cell cycle and increase sensitivity to radiotherapy. This study will provide the useful information on development of herbal medicine having radioprotective effects.

• 생약학회지
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• 제42권2호
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• pp.195-200
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• 2011

In this study, we investigated on antioxidative activity, tyrosinase inhibitory activity and nitric oxide(NO) production inhibitory activity in the hot water extract of Taraxacum coreanum and its fractions. The total polyphenol and flavonoid contents of the extract were found to be 65.42 mg/g and 9.83 mg/g, respectively. And the total polyphenol and flavonoid contents of the ethyl acetate fraction were found to be 168.23 mg/g and 31.92 mg/g, respectively. In DPPH radical scavenging ability, $SC_{50}$ values of the ethyl acetate and butanol fraction were exhibited 64.65 ${\mu}g/ml$ and 277.42 ${\mu}g/ml$, respectively. Moreover, ethyl acetate fraction showed higher inhibitory activity than other samples in tyrosinase inhibitory activity. In NO production inhibitory activity, the extract and its fractions showed NO production inhibitory effect. Especially, the ethyl acetate, chloroform and butanol fraction was exhibited higher NO production inhibitory activity than other samples. As a result, the ethyl acetate and butanol fraction from the water extract of T. coreanum could be applicable to functional materials for skin-related fields.

• Advances in Traditional Medicine
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• 제8권1호
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• pp.59-66
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• 2008

This study was undertaken to evaluate the antioxidant potential of A. lanata on oxalate mediated free radical toxicity in ethylene glycol induced calcium oxalate urolithic rats. Calcium oxalate (CaOX) stone was induced by 0.75% ethylene glycol in drinking water for 28 days. From $29^{th}$ day onwards, the CaOX urolithic rats were treated with A. lanata aqueous suspension (2,000 mg/kg body weight/dose/day) orally for another 28 days. At the end of experimental periods the animals were sacrificed, samples were collected and analyzed the lipid peroxidation product, protein oxidation product, enzymatic and non-enzymatic antioxidants in normal and experimental groups. Lipid peroxidation and protein oxidation products were significantly elevated while enzymatic and non-enzymatic antioxidant levels were significantly decreased in ethylene glycol induced CaOX urolithic rats when compared with control rats. The above alterations were reverted to near control in rats treated with aqueous suspension of A. lanata. This study suggests that A. lanata could prevent the free radical formation from calcium oxalate urolithiasis in rats and protecting the renal cells from oxidative injury.

• 대한한의학방제학회지
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• 제29권4호
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• pp.277-283
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• 2021

Objectives : We investigated the effects of Bojungikgi-tang (BJIGT) on P450 cytochrome enzyme and oxidative stress in the cells. Methods : We enrolled the HepG2 hepatocyte cell line to assess MTT assay, flow cytometer, and immunoblotting analysis. Expression of CYP450 was confirmed by immunoblotting analysis in the Huh7 cell line. Results : We determined that BJIKT markdely changed the expression of the CYP2C19, CYP2D6, and CYP2E1. Moreover, BJIKT inhibited the cell toxicity induced by arachidonic acid + iron treatment, as assessed by FACS analysis. BJIKT induced AMPK activation, which increased the phophorylation of ACC. Conclusions : This study verified the effects of BJIKT, on P450, ROS production, mitochondrial damage and AMPK signaling pathway, which might give us the scientific information about the traditional herbal prescription.

• 대한화장품학회지
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• 제40권1호
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• pp.29-36
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• 2014

철피석곡은 중국에서 전통의약식물로 잘 알려진 난과 식물 중 하나이다. 본 연구에서는 생물반응장치를 이용하여 조직 배양된 철피석곡에 대하여 화장품 성분으로써 응용 가치를 평가하였다. 이미 몇몇 보고된 논문에서 철피석곡은 항암, 상처 치유 그리고 면역기능증진 등의 약리학적 활성에 대한 연구가 진행되었다. 본 연구에서는 생물반응장치를 이용하여 조직 배양된 철피석곡의 원괴체상구체 및 다신초에 대하여 화장품 성분으로서 효능, 효과를 자연에서 자란 철피석곡과 비교 평가하였다. 항산화 활성 비교 실험 결과 철피석곡의 원괴체상구체는 조직배양된 다신초 및 철피석곡 지하부 및 지상부보다 항산화 효과가 우수하였다. 조직배양된 원괴체상구체의 총 페놀 및 총 플라보노이드 성분 또한 다른 다신초나 자연에서 자란 철피석곡 추출물보다 함유량이 높음을 알 수 있었다. 다른 한편으로는 피부 미백과 관련한 효과를 비교 조사한 결과, 조직 배양된 다신초가 자연에서 자란 철피석곡 지상부, 지하부 및 조직 배양된 원괴체상구체보다 타이로 시네이즈 활성 억제 및 멜라닌 생성 억제 효과가 우수하였다. 이러한 결과들은 피부개선을 위한 화장품 성분으로서 조직배양체의 부분별 응용 가능성을 제공할 수 있을 것으로 사료된다.

• 원예과학기술지
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• 제28권5호
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• pp.850-856
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• 2010

고혈당 쥐에(Sprague-Dawley rat) 각종 야채와 과일을 섭취(1g/kg body weight) 한 후 oral glucose tolerance test(OGTT)을 실시하였다. 0분, 5분, 15분, 30분, 60분, 90분, 120분 후에 blood glucose levels(${\Delta}BGLs$)을 측정하였다. Under glucose curve(${\Delta}AUCs$)은 OGGT의 120분 후 계산하였으며 과일과 야채의 Total phenolic content(TPC)과 anti-oxidant activity (AOA)는 Folin Ciocalteu and DPPH(2, 2-diphenyl-1-picrylhydrazyl)을 통하여 특정하였다. 실험의 마지막은 Pearson's correlations을 사용하여 TPC, AOA and ${\Delta}AUC$ 간의 상관관계를 분석하였으며 모든 통계수치는 unpaired Student's t-test를 실시하였다. 과일중에서는 탠저린, 자두, 배가 저혈당 효과를 보였으며 야채중에서는 푸른잎 머스타드와 양배투, 치커리, 브로콜리가 감소된 ${\Delta}BGLs$와 유효한 ${\Delta}AUC$수치를 보여서 저혈당 효과에 효과적이었다. 효과적인 ${\Delta}AUC$의 범위는 $5548.2{\pm}462.1$에서부터 $3823.3{\pm}282.0mg{\cdot}min/dL$이며, TPC와 AOA의 범위는 $0.063{\pm}0.00$에서부터 $0.913{\pm}0.14mg/g$ GAE, $01.05{\pm}0.08$에서부터 $75.46{\pm}0.06%$이다. 전체적으로 과일의 50%와 양체의 60-65%가 높은 TPC와 효과적인 AOA의 수치를 나타내었다. 우리는 이번 연구를 통하여 저혈당 효과가 있어 제2형 당뇨를 예방할 수 있는 과일과 채소류를 선별할 수 있었다.

• 생명과학회지
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• 제20권3호
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• pp.444-452
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• 2010

본 연구의 목적은 PS-2 (N141I) 알츠하이머 형질전환 모델 생쥐를 대상으로 트레드밀 운동이 뇌의 세포질과 미토콘드리아의 $A{\beta}$-42, cytochrome c, SOD-1, 2 and Sirt-3 단백질 발현에 미치는 효과를 알아보는데 있다. 우선 알츠하이머 형질전환 생쥐를 Non-Tg-sedentary (n=5), Non-Tg-treadmill exercise (n=5) 집단과 Tg-sedentary (n=5), Tg-treadmill exercise (n=5) 집단으로 구분하고 트레드밀 운동을 통한 신경보호 효과를 검증하기 위해 Tg와 Non-Tg집단에 12주간 트레드밀 운동을 수행한 후 인지능력을 살펴보고 뇌의 세포질과 미토콘드리아의 $A{\beta}$-42, cytochrome c, anti-oxidant enzymes (SOD-1, SOD-2)와 Sirt-3 단백질을 분석하였다. 먼저 트레드밀운동은 Tg 집단에서 인지능력의 개선을 나타냈으며 미토콘드리아의 $A{\beta}$-42와 세포질의 cytochrome c 단백질의 감소와 항산화 효소인 SOD-1, SOD-2를 유의하게 증가시켰다. 게다가 트레드밀 운동은 모든 집단에서 Sirt-3 단백질의 발현을 증가시켰다. 따라서 트레드밀 운동은 인지능력의 향상과 세포 내 스트레스를 유발하는 $A{\beta}$-42를 억제시켜 알츠하이머 질환을 개선시킬 수 있는 효과적인 방법이라고 생각된다.