• The Korea Journal of Herbology
• /
• v.35 no.4
• /
• pp.37-43
• /
• 2020

Objectives : Allium hookeri is a well-known traditional herbal remedy and its root used for treatment of inflammation and tumor. However, the mechanism of anti-inflammatory effect of Allium hookeri is still unknown. This study aims to examine the mechanism of anti-inflammatory effect of Allium hookeri on mouse macrophage cell line, RAW 264.7 cells. Methods : Anti-oxidant effect of water extract of Allium hookeri (WEAH) was measured by 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. 3- (4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed to determine the effect of WEAH on cell viability in RAW 264.7 cells. In addition, anti-inflammatory effect of WEAH was investigated in RAW 264.7 cells. Inflammation of RAW 264.7 cells induced by lipopolysarccharide (LPS) treatment and expression levels of inflammatory cytokine interleukin 1 β (IL-1β) and interleukin 6 (IL-6) gene were analyzed using quantitative reverse transcription PCR (qRT-PCR) analysis. Furthermore, the phosphorylation of inhibitor of nuclear factor kappa B (IκBα) after LPS treatment with WEAH-treated RAW 264.7 cells was confirmed by immunoblot analysis. Results : WEAH showed a strong anti-oxidant effect and no cytotoxicity to RAW 264.7 cells up to 2 mg/㎖ concentration. The LPS-induced mRNA expression levels of IL-1β and IL-6 were decreased by WEAH treatment. Furthermore, the LPS-induced phosphorylation of IκBα is attenuated by WEAH treatment. Conclusions : Through experimental demonstration of anti-oxidant and anti-inflammatory effects of WEAH, we suggest that Allium hookeri is a valuable material for prevention and treatment of various inflammatory diseases.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.26 no.4
• /
• pp.519-526
• /
• 2012

Recently, herbal flavonoids have been implicated for anti-cancer therapy. Flavonoids as a commonly known for their anti-oxidant activity, are contained in the herbal medicine as well as root of plants, vegetables, fruits, grains, tea, and wine. Kaempferol, a component of Polygonati rhizoma, a member of the herbal flavonoids, has been studied for anti-hypercholesterol, anti-hypertension and anti-diabetes. It is also known to be effective in anti-cancer therapy for breast, prostate and other type of cancers. However, the anti-cancer therapeutic mechanisms are pooly understood. Here, we investigated the molecular mechanism underlying kaempferol-induced anti-cancer effects using the human liver cancer cell lines, Hep3B, HepG2, and Sk-Hep-1, and human Chang liver cell as a control. As shown by the FACS analysis, measurement of caspase activity, DAPI and trypan blue staining, and DNA fragmentation assay, kaempferol induced apoptosis in the liver cancer cells with the greater potential in Hep3B cells than other liver cancer cells. In addition, we performed microarray analysis to profile the genome-wide mRNA expression regulated by kaempferol. Many of the apoptosis-related genes were significantly induced in kaempferol-treated Hep3B cells, in particular, the genes associated with MAPK cascade. Additionally, kaempferol induced the mRNA expression of genes involved in MKK7-JNK cascade, MKK3-p38 cascade, and caspase signaling pathway, which are all known to trigger apoptosis. Overall, our data suggest that kaempferol has anti-liver cancer effects by inducing apoptosis through the MKK7-JNK cascade, MKK3-p38 cascade, and caspase signaling pathways.

• Journal of the Korean Society of Safety
• /
• v.32 no.4
• /
• pp.22-27
• /
• 2017

In this study, the authors aim to evaluate a structural and fatigue safety of a new type anti-loose nut system with dual nuts composed of main nut and outer nut to enhance the long-term workability and durability so as to improve the performance of conventional anti-loose nut system. Also, a three-dimensional finite-element method analysis was performed to consider the actual geometry and material property of anti-loose nut system with dual nuts and the effect of static and dynamic loads and loading directions. The analytical results showed that the overall static and dynamic stress of the components of the anti-loose nut system with dual nuts were found to be less than that of the fatigue limit of Goodman-smith diagram and allowable stress of each materials, therefore the anti-loose nut system with dual nuts was sufficient to ensure a structural and fatigue safety.

• Preventive Nutrition and Food Science
• /
• v.21 no.4
• /
• pp.323-329
• /
• 2016

Achyranthes japonica Nakai (AJN) water extract has a variety of physiological properties, including anti-diabetic, anti-cancer, anti-inflammatory, anti-microbial, and anti-oxidative activities. In the present study, the inhibitory effects of AJN extract were investigated in high affinity immunoglobulin E receptor ($Fc{\varepsilon}RI$)-mediated KU812F cells activation. AJN extract showed suppressive effects on histamine release and intracellular calcium [$Ca^{2+}$]i elevation from anti$Fc{\varepsilon}RI$ antibody (CRA-1)-stimulated cells in a dose-dependent manner. Flow cytometric analysis showed that AJN extract treatment caused a dose-dependent decrease in the cell surface $Fc{\varepsilon}RI$ expression and the binding between the cell surface $Fc{\varepsilon}RI$ and the IgE antibody. Moreover, reverse transcription-polymerase chain reaction analysis showed that levels of the mRNA for the $Fc{\varepsilon}RI$ ${\alpha}$ chain was decreased by treatment with AJN extract. These results indicate that AJN extract may exert anti-allergic effects via the inhibition of calcium influx and histamine release, which occurs as a result from the downregulation of the binding of IgE antibody to cell surface $Fc{\varepsilon}RI$. This mechanism may occur through $Fc{\varepsilon}RI$ expression inhibition.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.10 no.1
• /
• pp.29-37
• /
• 2005

This paper presents a tuning-free conditional integration anti-windup strategy for induction machine with Proportional-Integral(PI) type speed controller. The on/off condition of integral action is determined by the frequency domain analysis of machine torque command without a prior knowledge of set-point changes. There are no tuning parameters to be selected by users for anti-windup scheme. In addition, the dynamic performance of the proposed scheme assures a desired tracking response curve with minimal oscillation and settling time even in the change of operating conditions. This algorithm is useful in many high performance induction machine applications not to allow the oscillation and overshoot of speed/torque responses. The main idea can be extended to general applications such as chemical processes and industrial robots.

• Journal of Positioning, Navigation, and Timing
• /
• v.11 no.3
• /
• pp.181-189
• /
• 2022

The public open signals from Global Navigation Satellite System (GNSS) including Global positioning system (GPS) are used widely by many peoples in the world except for the public regulated restriction signals which are encrypted. Nowadays there are growing concerns about GNSS signal spoofing which can deceive the GNSS receivers by abusing these open services. To counter these spoofing threats, many researches have been studied including array antenna techniques which can detect the direction of arrival by means of Multiple Signal Classification (MUSIC) algorithm. Originally the array antenna techniques were developed to countermeasure the jamming signal in electronic warfare by using the nulling or beamforming algorithm toward a certain direction. In this paper, we study the anti-spoofing techniques using array antenna to overcome the jamming and spoofing issues simultaneously. First, we will present the theoretical analysis results of spoofing signal response of Minimum Variance Distortionless Response (MVDR) algorithm in array antenna. Then the eigenvector algorithm of covariance matrix is suggested and verified to work with the existing anti-jamming method. The modeling and simulation are used to verify the effectiveness of the anti-spoofing algorithm. Also, the field test results show that the array antenna system with the proposed algorithms can perform the anti-spoofing function. This anti-spoofing method using array antenna is very effective in the view point of solving both the jamming and spoofing problems using the same array antenna hardware.

• Journal of Plant Biotechnology
• /
• v.50
• /
• pp.27-33
• /
• 2023

Zizyphus jujube is a plant in the buckthorn family (Rhamnaceae) that has been the subject of research into antibacterial, antifungal and anti-inflammatory properties of its fruit and seed. However, few studies have investigated its leaves. In this study, the anti-inflammatory activity of ZJL (an extract of Z. jujube leaf) was evaluated to verify its potential as an anti-inflammatory agent and SARS-CoV-2 medicine, using nitric oxide (NO) assay, RT-PCR, SDSPAGE, Western blotting, and UHPLC/TOFHRMS analysis. We found that ZJL suppresed pro-inflammatory mediators such as NO, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and tumor necrosis factor α (TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells. ZJL acted by inhibiting NF-KB and MAPK signaling pathway activity. We also confirmed that ZJL contains a phenol compound and flavonoids with anti-inflammatory activity such as trehalose, maleate, epigallocatechin, hyperoside, catechin, 3-O-coumaroylquinic acid, rhoifolin, gossypin, kaempferol 3-neohesperidoside, rutin, myricitrin, guaiaverin, quercitrin, quercetin, ursolic acid, and pheophorbide a. These findings suggest that ZJL may have great potential for the development of anti-inflammatory drugs and vaccines via inhibition of NF-κB and MAPK signaling in LPS-induced RAW264.7 cells.

• Journal of Pharmacopuncture
• /
• v.27 no.3
• /
• pp.223-233
• /
• 2024

Objectives: The phytochemicals in the aerial parts of Euphorbia paralias (also known as Sea Spurge) and their anti-inflammatory and antimicrobial activities were investigated. Methods: The methanolic extract was characterized using GC-MS and HPLC techniques. The anti-inflammatory feature was estimated through a Human Red Blood Cell (HRBC) membrane stabilization technique, while the antimicrobial feature was evaluated by the disc diffusion agar technique, minimum bactericidal concentration, and minimum inhibitory concentration (MIC) via micro-broth dilution method. Results: The GC/MS results demonstrated the existence of various phytochemicals, such as n-hexadecenoic acid, cis-11-eicosenoic acid, and methyl stearate, recognized for their anti-inflammatory and antibacterial features. The similarity of the phytochemical composition with other Euphorbia species emphasizes the genus-wide similarity. The anti-inflammatory activity exhibited a noteworthy inhibitory effect comparable to the reference drug indomethacin. The extract's antimicrobial potential was tested against a range of microorganisms, demonstrating significant action against Gram-positive bacteria and Candida albicans. The quantification of total phenolics and flavonoids further supported the therapeutic potential of the extract. Conclusion: The methanolic extract from E. paralias emerges as a successful natural source of important active constituents with potential applications as anti-inflammatory and antimicrobial agents. This research provides a first step to valorize Euphorbia paralias insights as a source of worthwhile phytochemicals that have potential applications in the pharmaceutical industry.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2002.05a
• /
• pp.829-832
• /
• 2002

With the increase of storage density, high rotational speed and high access technologies in optical disk drive, mechanical issues, mainly noise and vibration, become critical. Up to now the researches of rubber mount for anti-vibration focused on how to calculate the static and the dynamic stiffness of rubber mount and leaved out of consideration of the dimensional tolerance of rubber mount for anti-vibration. This paper presents the effects of dimensional tolerance of rubber mount for anti-vibration on the dynamic characteristics of optical disk drive by finite element analysis and dynamic test. The relation between dimensional tolerance and dynamic characteristics of optical disk drive is observed and discussed.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.12 no.9
• /
• pp.661-667
• /
• 2002

With the increase of storage density, high rotational speed and high access technologies in optical disk drive, mechanical issues, mainly noise and vibration, become critical. Up to now the researches of rubber mount for anti-vibration focused on how to calculate the static and the dynamic stiffness of rubber mount and loaned out consideration of the dimensional tolerance of rubber mount for anti-vibration. This paper presents the effects of dimensional tolerance of rubber mount for anti-nitration on the dynamic characteristics of optical disk drive by finite element analysis and dynamic test. The relation between dimensional tolerance and dynamic characteristics of optical disk drive is observed and discussed.