• Journal of Microbiology and Biotechnology
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• v.1 no.4
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• pp.266-273
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• 1991

Hirudin, a 65-amino acid protein isolated from the salivary gland of the bloodsucking leech, Hirudo medicinalis, is a potent thrombin-specific inhibitor and blocks the thrombin-mediated conversion of fibrinogen to fibrin in clot formation. We have studied the gene expression and secretion of hirudin in yeast. Saccharomyces cerevisiae. A gene coding for hirudin was synthesized based on the amino acid sequence and cloned into a yeast expression vector $YEG{\alpha}-1$ containing the ${\alpha}-mating$ factor pre-pro leader sequence and galactose-inducible promoter, GALl0. Recombinant S. cerevisiae was found to secrete biologically active hirudin into the extracellular medium. The secreted recombinant hirudin was recovered from the culture medium and purified with ultrafiltration and reverse phase high performance liquid chromatography. Approximately 1 mg of hirudin per liter was produced under suboptimal culture conditions and brought to about 90% purity in two steps of purification.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.53 no.1
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• pp.31-37
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• 2004

In this paper, the analysis result of the switching surge in substation including GIS(Gas Insulated Switchgear) has been described using the EMTP(Electro Magnetic Transient Program) program. After performing the modeling of the input data, we have reviewed the energization overvoltages of GCB(Gas Circuit Breaker) without closing resistor in 345kV network, according to the conditions of simultaneously operating switch and statistically operating switch. Firstly, it can be known that the energization overvoltage of single line is higher than that of parallel energized line. Secondly, the maximum energization overvoltage obtained in statistics switch is higher than one in simultaneous switch. From these results, it can be known that the phase-to-earth energization overvoltages of $\alpha$-$\beta$ section are less than 2.2 p.u when closing resistor is eliminated. Therefore, it is possible to consider the circuit breaker eliminated the closing resistor in $\alpha$-$\beta$ section.

• Journal of the Korean Ceramic Society
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• v.30 no.7
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• pp.571-577
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• 1993

The effect of $\alpha$-Al2O3 seeding on preparation of zirconia/alumina gel fragment prepared by sol-gel processing was characterized through XRD, SEM, TG/DTA and IR analysis. Aluminum isopropoxide and zirconium butoxide were used as starting materials. $\alpha$-Al2O3 seeding restrained grain growth of alumina and zirconia, and decreased tetragonal to monoclinic phase transformation of zirconia on cooling. Therefore, fine zirconia-toughened alumina composite having the relative sintered density of about 98% of theoretical at 140$0^{\circ}C$ for 2h could be obtained.

• Journal of the Korean Ceramic Society
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• v.28 no.9
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• pp.705-711
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• 1991

Alumina precursor sol was obtained by the reaction of Al(OC4H9)3 and acetylacetone in the solvent followed by the partial hydrolysis. This sol was measured by viscosity and the effect of pH. The powders obtained from this sol were calcined at the various temperatures. The transition of crystals and crystal state were investigated at the various temperatures. The powders dried at 90$^{\circ}C$ showed amorphous and ${\gamma}$-Al2O3 at 900$^{\circ}C$, ${\alpha}$-Al2O3 mono-phase at 1050$^{\circ}C$ respectively. As a result of Al27-MASNMR analysis, amorphous and ${\alpha}$-Al2O3 powders showed 6-coordinated Al, ${\gamma}$-Al2O3 4-coordinated Al respectively.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.07b
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• pp.822-825
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• 2003

Maxwell displacement current (MDC) measurement has been employed to study the dielectric property of Langmuir-films. A method for determining the dielectric relaxation time $\tau$ of floating monolayers on water surface is presented. WC flowing across monolayers is analyzed using a rod-like molecular model. It is revealed that the dielectric relaxation time $\tau$ of monolayers in the isotropic polar orientational phase is determined using a liner relationship between the monolayer compression speed ${\alpha}$ and the molecular area Am. Compression speed ${\alpha}$ was about 30, 40, 50mm/min.

• JSTS:Journal of Semiconductor Technology and Science
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• v.15 no.6
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• pp.671-677
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• 2015

A novel phase interpolator (PI) based linear model of multipath ring oscillator (MPRO) is described in this paper. By modeling each delay cell as an ideal summer followed by a single pole RC filter, the oscillation frequency is derived for a 4-stage differential MPRO. It is analytically proved that the oscillation frequency increases with the growth of the forwarding factor ${\alpha}$, which is also confirmed quantitatively through simulation. Based on the proposed model, it is shown that the power to frequency ratio keeps constant as the speed increases. Running at the same speed, a 4-stage MPRO can outperform the corresponding single-stage ring oscillator (SPRO) with 27% power saving, making MPRO with a large forwarding factor ${\alpha}$ an attractive option for lower power applications.

• Journal of Environmental Science International
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• v.12 no.1
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• pp.77-80
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• 2003

The interaction of mastoparan B, a cationic tetradecapeptide amide isolated from the hornet Vespa basalis, with phospholipid bilayers was studied with synthetic mastoparan B and its analogue with Ala instead of hydrophobic 12th amino acid residue in mastoparan B. MP-B and its derivative, [12-Ala]MP-B were synthesized by the solid-phase peptide synthesis method. MP-B and its analogue, [12-Ala]MP-B adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, the peptides took an $\alpha$-helical structure. The two peptides interacted with neutral and acidic lipid bilayers. These results indicated that the hydrophobic face in the amphipathic $\alpha$-helix of MP-B critically affected the biological activity and helical content.

• Proceedings of the KIEE Conference
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• 1995.11a
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• pp.420-422
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• 1995

PVDF (polyvinylidene fluoride) has at least from known crystalline structure ( ; they are referred to as the $\alpha$, $\beta$, $\gamma$ and $\alpha_p$ phase or forms II, I, III and $IV_p$). In this study, the manufactured PVDF thin films through vapor deposition method had for II ( ; the substrate temperature at 30$^{\circ}C$). The dielectric behavior of poly(vinylidene fluoride) is affected by orientation and crystal modification. The very high value of the dielectric constant for high temperature conditioned film is believed to be due to the orientation effect. The loss peak caused by molecular motion of the molecules in crystalline regions.

• Journal of the Korean Society for Heat Treatment
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• v.10 no.2
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• pp.121-127
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• 1997

In order to elucidate thermal stability of Fe-$Fe_2Ti$ eutectic structure, the initial several structures have been investigated in the changes of coarsening and spheroidization during prolonged annealing under the eutectic temperature. The results are as follows: 1) The rate constant of coarsening and spheroidization was formulated as $S^{-n}-S_0^{-n}=k{\cdot}t$, where S is the total area of the interface between ${\alpha}$ and C($Fe_2Ti$) per unit volume, $S_0$ is initial value and k is the rate constant. 2) The coarsening and spheroidization mechanism was described by Ostwald ripening and controlled by diffusion of Ti-atom in ${\alpha}$-phase. 3) The spheroidization rate constant in eutectic lamellar structures was depended upon annealing temperature and showed the Arrhenius relation. The activation energy for spheroidization of lamellar structure was 365 kJ/mole.

• Electrical & Electronic Materials
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• v.10 no.10
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• pp.995-999
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• 1997

For melt-spun Fe-Nd-C alloy variation of phase development and magnetic properties with the variety of small Al addition was investigated. As the amount of Al addition increased the amorphization of Fe$_{14}$/Nd$_{2}$/C or $\alpha$-Fe was retarded and the residual Fe$_{14}$/Nd$_{2}$/C or $\alpha$-Fe did not eliminated after heat treatment resulting in poor coercivities of the ribbons. The grain size of Fe$_{14}$/Nd$_{2}$/C increased with the increase of Al addition and the abnormal grain was often found out in the ribbon heat treated for more then 10 min. The enhancement of coercivity was the highest with the addition of 0.5at%Al. As the amount of Al addition increase the coercivities dropped rapidly. The highest coercivity obtained so far is 13.9kOe which about 16% higher than that(11.2kOe) obtained from the Al-free specimen.