Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of the Interactive Characteristic of Environmental Toxic Peptide and Phospholipid

환경 독성 Peptide의 인지질과의 상호 작용 특성 분석

  • Published : 2003.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

The interaction of mastoparan B, a cationic tetradecapeptide amide isolated from the hornet Vespa basalis, with phospholipid bilayers was studied with synthetic mastoparan B and its analogue with Ala instead of hydrophobic 12th amino acid residue in mastoparan B. MP-B and its derivative, [12-Ala]MP-B were synthesized by the solid-phase peptide synthesis method. MP-B and its analogue, [12-Ala]MP-B adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, the peptides took an $\alpha$-helical structure. The two peptides interacted with neutral and acidic lipid bilayers. These results indicated that the hydrophobic face in the amphipathic $\alpha$-helix of MP-B critically affected the biological activity and helical content.

Keywords

References

  1. Ho, C. L. and L. L. Hwang, 1991, BioChem. J., 274, 453-457. https://doi.org/10.1042/bj2740453
  2. Barnette, M. S., R. Daly, and B. Weiss, 1983, BioChem. Phannacol., 32, 2929-2934. https://doi.org/10.1016/0006-2952(83)90398-2
  3. Argiolas, A. and J. J. Pisano, 1983, Biol. Chem., 258, 13697-13702.
  4. Higashijima, S., S. Uzu, T. Nakajima, and E. M. Ross, 1988, Biol. Commun., 263, 6491-6497.
  5. Okmura, K., K. Inui, Y. Hirai, and T. Nakajima, 1981, Biomed. Res., 2, 450-457. https://doi.org/10.2220/biomedres.2.450
  6. Park, N. G., Y. Yamato, S. Lee, G. Sugihara, J. S. Park, and S. W. Kang, 1996, Bull. Korean Chem. Soc., 17, 239-246.
  7. Gad, A. E., B. L. Silver, and G. D. Eytan, 1982, Biochirn. Biophys. Acta, 690, 124-128. https://doi.org/10.1016/0005-2736(82)90246-2
  8. Surewics, R. M., R. M. Epand, W. J. Vail, and M. A. Moscarelo, 1985, Biochim. Biophys. Acta, 820, 310-306.
  9. Uster, P. S. and D. W. Dreamer, 1985, Biochemistry, 24, 1-8. https://doi.org/10.1021/bi00322a001
  10. Wang, C. Y. and L. Huang, 1984, Biochemistry, 23, 4409-4411. https://doi.org/10.1021/bi00314a026