• Toxicological Research
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• v.25 no.4
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• pp.243-251
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• 2009

Present study was conducted to evaluate the anti oxidative activity of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidative damage. The antioxidative activity of methanolic (MeOH) extract of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidation, including liposome oxidation, deoxyribose oxidation, protein oxidation, chelating activity against metal ions, scavenging activity against hydrogen peroxide, scavenging activity against hydroxyl radical and 2'-deoxyguanosine (2'-dG) oxidation were investigated. The MeOH extract of the Agrimonia pilosa-Ledeb leaves exhibited high anti oxidative activity in the liposome model system. Deoxyribose peroxidation was inhibited by the MeOH extract of the Agrimonia pilosa-Ledeb leaves and MeOH extract of the Agrimonia pilosa-Ledeb leaves provided remarkable protection against damage to deoxyribose. Protective effect of MeOH extracts of the Agrimonia pilosa-Ledeb leaves on protein damage was observed at $600{\mu}g$ level (82.05%). The MeOH extracts of the Agrimonia pilosa-Ledeb leaves at $300{\mu}g$ revealed metal binding ability (32.64%) for hydrogen peroxide. Furthermore, the oxidation of 2'-deoxyguanosine (2'-dG) to 8-hydroxy-2-deoxyguanosine (8-OH-2'dG) was inhibited by MeOH extracts of the Agrimonia pilosa-Ledeb leaves and scavenging activity for hydroxyl radical exhibited a remarkable effect. From the results in the present study on biological model systems, we concluded that MeOH extract of the Agrimonia pilosa-Ledeb leaves was effective in the protection of non-lipids against various oxidative model systems.

• Korean Journal of Medicinal Crop Science
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• v.16 no.3
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• pp.139-143
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• 2008

The antioxidant activities of Agrimony (Agrimonia pilosa L.) and Chinese lizardtail (Saururus chinensis Baill) according to extraction methods were measured. SOD-like activity showed greater antioxidant effects with ethanol solvent than those with water. Ethanol extracts of Agrimony leaves showed the highest SOD-like activity of 94.4%. SOD-like activity differed according to the extraction solvents. The contents of polyphenolic compounds were higher in water extracts than those in ethanol extracts. The contents were 161.4 mg for Agrimony roots, 100.2 mg for Agrimony leaves, and 79.1 mg for Agrimony stalks in order. EDA in Agrimony leaves that were highest among medicinal plants were 83.4% in the water extract and 81.7% in the ethanol extract. The anticancer effects of the extracts by water and ethanol from Agrimony and Chinese lizardtail were experimented. The growth of stomach cancer cells, SNU-719 was inhibited 94.5% by the hexane fractions of Agrimony and also the growth of liver cancer cells, Hep3B was inhibited 83.2% by the hexane fractions of Agrimony, while the growth of normal cell, DC2.4 was not affected.

• Korean Journal of Medicinal Crop Science
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• v.8 no.2
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• pp.129-133
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• 2000

The experiment was conducted to increase germination of Agrimonia pilosa seeds, one of the natural medicinal plants, by various treatments, and also the effects of seed treatments on the growth of plants after sowing in field. The optimum temperature for germination of A. pilosa seeds was $20^{\circ}C$ after 20 days stratification duration at $4^{\circ}$, the germination rate than highest, 86% at that temperature. The stratification increased germination rate in all the temperatures treated, shortened $26{\sim}32$ days of germination period and promoted more 20% germination rate than the other treatments. Flowing water, low temperature, $GA_3$ and NaOCl were not effective for germination and high temperature reduced germination rate. At the early growth stage, the plant height was taller $3{\sim}4\;cm$, one or more leaves were increased by the stratification treatments, and also stratification increased the number of leaves per plants 10 or more leaves compare with other treatments at harvesting stages. By stratification, the flowering date was 5 days earlier, June 18th than other treatments due to earlier sprouting.

• Research in Plant Disease
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• v.14 no.3
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• pp.201-204
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• 2008

We've been involved in collection and domestication of Agrimonia pilosa from 2005 to 2007. We observed agrimony powdery mildew caused by Sphaerotheca aphanis. The powdery mildew developed twice a year, in May to June and mid August to September on whole plants of A. pilosa plants, that is, leaves, stem and floral part. We sprayed the Cooking Oil & Yolk mixture (COY) to control the agrimony powdery mildew. When COY was single-sprayed to control agrimony powdery mildew, its control value was 26.9%. However when it was double-sprayed and triple-sprayed at seven days interval, its control value increased up to 80.8 and 82.7%, respectively. Upon the first spray, symptoms of powdery mildew disappeared within three days and suppressive effect of COY was persisted for only seven days. Thereafter, powdery mildew was relapsed to develop. However, on double to triple sprays, powdery mildew was suppressed more than 28 days after final treatment. Therefore, it is concluded that double spray of COY at seven days interval can control effectively agrimony powdery mildew.

• Korean Journal of Plant Resources
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• v.15 no.3
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• pp.243-249
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• 2002

This experiment was conducted to find out the effects of livestock excrements application on the growth and yield of leaf, stem and seed in Agrimonia pilosa Ledeb. the results were summarized as follow. In the soil after experiment, P2O5 and K contents were high in order of cattle droping 〈 pig droping 〈 chicken droping treatment. The plant height was increased cattle dropping〈 pig dropping〈 chicken dropping treatment plot. Number of stems was inclined to increase in the plot of 2,000 kg/10a application of chicken dropping and cattle dropping. Number of leaves was also increased 21.8∼51.6% by application 3,000 kg/10a of chicken and cattle dropping, and application 2,000 kg/10a of pig dropping, respectively. Dry weight of aerial part was high in order of 1,000〈 3,000〈 2,000 kg/10a〉control, on occasion 2,000 kg/10a treated, that was highest in the application of chicken dropping. Yield of seeds was also increased to 61％ and 86% at the 2,000 kg/10a plot of pig and chicken dropping, respectively. While yield of seeds was increased 50％ at the 3,000 kg/10a application of cattle dropping. Flowering ratio was 49.1％ in control, 55.6, 58.9 and 68.3% in cattle, pig and chicken dropping with 2,000 kg/10a, respectively, on June 22. Flowering ratio was the highest in the plot of chicken dropping with 2,000 kg/10a.

• Korean Journal of Pharmacognosy
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• v.17 no.4
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• pp.286-291
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• 1986

Forty herbal drugs which are described to have potential antitumor activity were solvent-fractionated with petroleum ether, ether and ethyl acetate in sequence. The cytotoxic activity was mostly shown in the ether fraction(40.54%) and petroleum ether fraction (35.15%), but scarcely in the water phase (10.8%), meaning that most of the active components had less polar property. Twenty-seven percent of the drugs tested were active, which is higher value than 10.4% of the random sampled drugs The drugs possessing the $ED_{50}$ values less than $10{mu}g/ml$ were the roots of Lithospermum erythrorhizon, Curcuma domestica, Salvia miltiorrhiza, Astragalus membraneceus and Scutellaria indica, the leaves of Panax ginseng, S. indica and Liriodendron tulipifera, the barks of Picrasma ailanthoides and Rhus vernifera, the herbs of Agrimonia pilosa and Siegesbeckia pubescens the seeds of Tricosanthes kirilowii, P. ailanthoides, and the stem of P. ginseng.

• Korean Journal of Medicinal Crop Science
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• v.15 no.6
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• pp.371-390
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• 2007

Arthropod pests were surveyed from 132 herb species of 121 genera in 50 families of 32 orders at herb garden of Sancheong-gun Agricultural Development Technology Center in Sancheong, Gyeongsangnamdo province from October, 2005 to November, 2006. Ninety eight arthropod pests of 86 genera in 44 families of 9 orders were collected. Although less than 5 arthropod pests were collected from each herb, more than 5 arthropod pests were occurred on Hibiscus mutabililis, Peucedanum japonicum, Aralia cordata, Fraxinus rhynchophylla, Angelica decursiva, Rhaponticum uniflorum, Lonicera japonica, Betula platyphylla and Agrimonia pilosa. Out of collected pests, 98.5% of them damaged leaves of medicinal plants. The highest number of arthropod pests was recorded in May representing 36 species of 32 genera in 20 families of 6 orders. Most of them were collected from less than 5 medicinal plants. However, Atractomorpha lata, Dolycoris baccarum, Myzus persicae, and Nysius plebejus occurred on 42., 22, 20, and 15 medicinal plants, respectively. Atractomorpha lata and Myzus persicae had broad host ranges and seriously damaged. Aphis gossypii was dominant insect pest in May compared with Dolycoris baccarum in June. Atractomorpha lata in August and September, and Myzus persicae in October.