• Journal of The Korean Society of Grassland and Forage Science
• /
• v.28 no.1
• /
• pp.1-6
• /
• 2008

Korditwo is a new orchardgrass(Dactylis glomerata L.) variety developed by the National Institute of Animal Science(NIAS) in 2005. To develope the new variety of orchardgrass, 5 superior clones, Dg8533, Dg9518, EDg9133, U8504 and U8519, were selected and polycrossed for seed production. That synthetic variety was designated newly as "Korditwo", and it's agronomic growth characteristics and forage productivity were examined at Suwon from 2001 to 2002, and regional yield trials were conducted in Suwon, Pyungchang, Youngju, Iksan, and Jeju from 2003 to 2005, respectively. "Korditwo" showed medium type growth habit in fall and spring and short in length of flag leaf and upper internode. Plant height of "Korditwo" was similar to that of standard variety, "Ambassador" and heading date was 4 days later than 14th May compared to Ambassador. Characteristics such as winter hardiness, lodging tolerance, disease resistance of "Korditwo" were stronger or better than those of Ambassador, specially in winter hardiness and lodging tolerance. "Korditwo" showed 10% higher dry matter yield(11.3 ton/ha) compared to Amabassador. Nutritive value was appeared to be similar in both varieties.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.14
• /
• pp.6039-6046
• /
• 2015

Aims: To investigate effect of metallic nanoparticles, silver (AgNPs) and gold nanoparticles (AuNPs) as antitumor treatment in vitro against human breast cancer cells (MCF-7) and their associated mechanisms. This could provide new class of engineered nanoparticles with desired physicochemical properties and may present newer approaches for therapeutic modalities to breast cancer in women. Materials and Methods: A human breast cancer cell line (MCF-7) was used as a model of cells. Metallic nanoparticles were characterized using UV-visible spectra and transmission electron microscopy (TEM). Cytotoxic effects of metallic nanoparticles on MCF-7 cells were followed by colorimetric SRB cell viability assays, microscopy, and cellular uptake. Nature of cell death was further investigated by DNA analysis and flow cytometry. Results: Treatment of MCF-7 with different concentrations of 5-10nm diameter of AgNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $6.28{\mu}M$, whereas treatment of MCF-7 with different concentrations of 13-15nm diameter of AuNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $14.48{\mu}M$. Treatment of cells with a IC50 concentration of AgNPs generated progressive accumulation of cells in the S phase of the cell cycle and prevented entry into the M phase. The treatment of cells with IC50 concentrations of AuNPs similarly generated progressive accumulation of cells in sub-G1 and S phase, and inhibited the entrance of cells into the M phase of the cell cycle. DNA fragmentation, as demonstrated by electrophoresis, indicated induction of apoptosis. Conclusions: Our engineered silver nanoparticles effectively inhibit the proliferation of human breast carcinoma cell line MCF-7 in vitro at high concentration ($1000{\mu}M$) through apoptotic mechanisms, and may be a beneficial agent against human carcinoma but further detailed study is still needed.

• Journal of Food Hygiene and Safety
• /
• v.29 no.4
• /
• pp.253-259
• /
• 2014

This study was conducted to investigate survival of Bacillus cereus (B. cereus) on stainless steel and polyvinyl chloride (PVC) and its transfer from two material to lettuce. The stainless steel and PVC were innoculated with B. cereus and stored at 6 combination conditions (temperature : $20^{\circ}C$ and $30^{\circ}C$, relative humidity (RH) : 43%, 69%, and 100%). Although the total numbers of B. cereus at RH 43% and RH 69% were reduced by 3.53-4.00 log CFU/coupon within 24 h regardless of material type, the spore numbers of B. cereus was lasted at 3.0 log CFU/coupon. When two materials were stored at $30^{\circ}C$, RH 100%, the spore numbers of B. cereus was rapidly increased by 3.0 log CFU/coupon. In addition, the reduction rate of B. cereus was decreased in the presence of organic matter. Transfer rate of B. cereus from surface of stainless steel and PVC to lettuce was increased by 10 times in the presence of water on the lettuce surface. As a result of this study, the presence of B. cereus on produce contact surfaces can increase the risk of cross-contamination. Thus, it is important that the packing table and conveyer belt in post harvest facility should be properly washed and sanitized after working to prevent cross-contamination.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.8 no.1
• /
• pp.30-42
• /
• 2006

This experiment was conducted to investigate current situation and plant grouping by light and watering of mingled several cacti and succulents in a pot. The main 11 cacti are consist of Myrtillocactus geometrizans (Mart.) Cons., Gymnocalycium mihanovichii var. friedrichii Werd., Marginatocereus marginatus(DC.) Backbg., Eriocactus leninghausii (Hge. Jr.) Backbg, Notocactus scopa (Spreng.) Berg., Lobivia nealeana Backbg., Mammillaria elongata var. intertexta (DC.) SD., Pseudoespostoa (Vp1.) Backbg., Mammillaria albilanata Backbg., Mammillaria klissingiana Bod. and Espostoa lanata (HBK.) Br. & R. The main 10 succulents are Kalanchoe thyrsifolia Harv., Portulacaria afra var. foliis-variegatis Jacobs., Crassula perforata Thunbg., Echeveria pulvinata Rose, Aeonium arboreum cv. atropurpureum., Echeveria lauii Moran & J. Meyran, Pachypodium lamerei Drake, Sedum rubrotinctum cv. Aurora Boom., Aloe mitriformis Mill., and Euphorbia coerulescens Haw. The cacti were native to Mexico, Brazil, Peru, Paraguay, Ecuador, etc. The sucullents were native to Mexico, Africa, Gran Canaria, Madagascar, etc. Most of all the cacti and sucullents in the experiment are growing well under full sun or light shade, however Portulacaria afra var. foliis-variegatis Jacobs, and Sedum rubrotinctum cv. Aurora Boom. did not influenced by light. And all the plants require a little or moderate watering. Thus, planting by similar required water and light, when growing in a pot of several cacti and succulents, might be decreased the rate of death causing by their different plant physiology.

• The Korean Journal of Mycology
• /
• v.12 no.3
• /
• pp.93-98
• /
• 1984

The effects of cultural conditions on the mycelial growth and fruit-body formation and the yield of Pleurotus sajor-caju grown in rice straw medium were examined. The change of content of chemical components of mushroom and the medium during the cultivation period was also examined. The growth of mycelium was best in the medium sterilized for 60 min at $1.2kg/cm^2$ with a moisture content of 60%. The optimum pH and temperature for mycelial growth were 6.0 and $25^{\circ}C$, respectively, whereas the optimum pH and temperature for fruit-body formation ranged from 5.0 to 6.0 and from 20 to $30^{\circ}C$, respectively. Rice bran and starch cake were better than the other additives used for both mycelial growth and fruit-body formation. The optimum concentration of rice bran in the medium was 10%. The total yield of mushroom was $20.2kg/m^2$, 66% of which was prouduced from the first and second cropping period. As the harvest proceeded, the crude fiber cotent increased in the mushroom, whereas it decreased in the medium. The content of free sugar and crude ash, on the other hand, decreased in the mushroom, whereas it increased in the medium. The content of the other chemical components examined decreased in both the mush­room and the medium.

• Journal of agricultural medicine and community health
• /
• v.4 no.1
• /
• pp.71-80
• /
• 1979

Paragonimiasis is a common trematodiasis of medical importance and one of public health problems in many areas of sob thern part of Korea. During the last sixty years, several on the prevalence among the Korean population have been carried out by various investigators, although the general distribution and prevalences of paragonimiasis are now yet clearly determind. This study was undertaken to evaluate the status of human paragonimiasis in Kang Hwa Gun, Kyung gi Province, Korea by the use of intradermal screening test whih Veronal buffered saline antigen. A total number of 208 residents (male 119, female 89) of all age group in Kilgeong-Ri, Yangdo Myun, Kang Hwa Gun were examined by the intradermal test with paragonimus antigen. The infestation rate of crayfish with Paragonimus metacerearia was also examine in Konneung and Jeoungha village, Kilgeong-Ri from March 1973 to October 1978 by the interval of two to five years. 1. In the resnlt of 208 residents, Kilgeong-Ri, the positive rate showed in 29.3% in as a whole(36.1% in male, 20.2% in female). The highest positive rate showed in 10 to 19 age group(41.5%). 2. Among the residents in Kilgeong-Ri, Paragonimus eggs were detected in 11 residents by the sputum and the stool examinations without the intradermal test result. 3. The infestation rate of crayfish with Paragonimus inetacercaria was 90.6%(116 out of 128 crayfishes) in Konneung village, and 61.5%(24 out of 39) crayfishes) in Jeongha village, Kilgeng-Ri. 4. The fluctation of the infestation tate and density of crayfish with Paragonimus metacercaria was decreased by the interval of two to five years in Konneung and Jeongha village in Kilgeong-Ri.

• The Korean Journal of Mycology
• /
• v.18 no.3
• /
• pp.149-157
• /
• 1990

Five strains of Armillaria mellea were collected from the forest of Chonbuk province and isolated from the tissue of fruit bodies. Nutritional and environmental characteristics of mycelial cultivation and rhizomorph production of Armillaria mellea isolated were determined in sawdust media, woody inocula and soils. The sawdust media of Styphnolobium japonicum, Culhamia simplex, Populus monilifera and Populua davidiana were proper for mycelial growth. The ranges of optimum pH, temperature and moisture content for mycelial growth were in the range of $4.5{\sim}5.0$, ${\sim}25^{\circ}C$ and $65{\sim}70%$, respectively. Among the various additives and inorganic salts added, 10% rice bran and 3% $CaCO_3$ were effective to mycelial growth. The woody inocula of Styphnolobium japonicum, Culhamia simplex, Quercus acutissima and Quercus veriabilis were proper for rhizomorph production. The ranges of optimum pH, moisture content and temperature for rhizomorph production were in the range of $4.5{\sim}4.9,$ $45{\sim}55%\;(w/w)$ and $20{\sim}24^{\circ}C$, respectively. Distribution of rhizomorphs in soil was varied with depth, but the main concentration occurred in the range of $7.5{\sim}12.5\;cm$. They were rarely found below 25.0 cm.

• Journal of Photoscience
• /
• v.11 no.3
• /
• pp.115-120
• /
• 2004

For the expression study of antioxidant isoenzyme genes in rice (Oryza sativa L.) plants, extensive searches for genes of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) isoforms were performed through the GenBank database. The genes for two cytosolic and one plastidic CuZn-SOD, one Fe-SOD, two Mn-SOD, two cytosolic and two chloroplastic (stromal and thylakoid) APX, and three CAT isoforms were available in japonica-type rice. These isoforms were named as cCuZn-SOD1, cCuZn-SOD2, pCuZn-SOD, Fe-SOD, Mn-SOD1, Mn-SOD2, cAPXa, cAPXb, Chl_sAPX, Chl_tAPX, CATa, CATb, and CATc, respectively. Since they shared a high degree of homology in the nucleotide and amino acid sequences, the gene-specific primers for the genes were designed directly from their full-length cDNAs found in the database except for the CATa gene. These primers were used in the RT-PCR analysis to investigate the differential expression of antioxidant isoenzyme genes in rice plants from the seeds irradiated with low doses (2, 4, 8, and 16 Gy) of gamma-radiation. The gammairradiation slightly increased the transcripts of pCuZn-SOD, while those of Fe-SOD, cAPXb, and CATb decreased. However, no substantial differences were observed in the expression of all the isoenzyme genes between the control and irradiated groups. In this study, gene specific primers for thirteen SOD, APX and CAT isoenzymes were constructed from the full-length cDNAs. The results of RT-PCR analysis obtained by using these primers suggests that the expression levels of SOD, APX, and CAT isoenzyme genes in rice seedlings were hardly affected by gamma-irradiation at the seed stage.

• The Korean Journal of Mycology
• /
• v.9 no.3
• /
• pp.123-128
• /
• 1981

Three edible species, e.g. Lyophyllum sp. and Armillaria mellea introduced from Japan in 1979 and Auriaularia auricula-judae from Taiwan in 1978 were selected for artifcial cultivation. The media, MSDS for Lyophyllum sp, MHS and PDS for A. auricula-judae, and MSDS and MHS for A. mellea were selected. The range of optimum pH of Lyophyllum sp. was 6.0 and pH 8.0 for A. auricula-judae and pH 6.0 to 7.0 for A. mellea. The range of optimum temperature for the mycelial growth of Lyophyllum sp. was 25 to $30^{\circ}C,\;30^{\circ}C$ for A. auricula-judae and $25^{\circ}C$ for A. mellea. When the moisture content 75 to 80% (w/w) in the substrates, the mycelial growth of Lyophyllum sp. and A. auricula-judae, was rapid. 65% (w/w) for A. mellea. As the spawning substrates, wheat for Lyophyllum sp. oak sawdust for A. auricula-judae and poplar sawdust for A. mellea were selected, respectively. In this experiment, yields of Lyophyllum sp. and A. auricula-judae were high on the poplar sawdust added 20% (w/w) of rice bran. Lyophyllum sp. and A. auricula-judae for sources of new edible mushrooms seems to be possible.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.1
• /
• pp.577-586
• /
• 2018

In this study, traffic safety technology was developed for rural areas by reviewingthe relevant literature and data from the Traffic Accident Analysis System for the Chungcheong region.The goal is to reduce traffic accidents in small regional cities and rural areas in Korea. A road shoulder recognition light was developed to fit the pedestrian characteristics of the people using transportation in rural areas. It also minimizes damage to crops due to light pollution from traffic lights and street lights, and it supplements problems of damage from collision with vehicles and agricultural machines. The efficiency of the technology developed in this study was verified by comparing and analyzing the number of traffic accidents and the saved cost before and after its installation. A test bedwas established based on rural areas and is being evaluated for its applicability and effectiveness. It is expected that the reliability of such facilities could be improved through continuous studies, data collection, and analysis.