• 한국미생물·생명공학회지
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• 제19권4호
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• pp.315-318
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• 1991

Pullulanase 생산력이 높은 세균 No.S-76을 토양으로부터 분리하였다. 분리된 균은 0.4~$0.6\times 0.8$~1.4 $\mu\textrm{m}$의 크기의 gram음성, 간균으로서 운동성이 있으며, 여러가지 특성을 조사한 결과 Bergey의 세균분류 동종법에 따라 Aeromonas caviae로 동정되었다. 본 균의 pullulanase 생산배지로서는 탄소원은 1 pullulan, soluble strach 또는 corn starch가, 질소원으로는 0.5% yeast extract 또는 peptone이 적당하였으며 initial pH는 9.0의 배지가 가장 좋았으며 $32^{\circ}C$에서 2일간 배양이 적당하였다.

• 미생물학회지
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• 제36권3호
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• pp.173-180
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• 2000

부산의 가물치 양식장으로부터 분리된 A. veronii bv. sobria 와 A. caviae를 대상으 로 16S-23S rRNA Intergenic Spacer Region을 cloning 하여 염기서dufd을 분석하였다. A. veronii bv. sobria 는 4그룹의 band pattern이 형성되었고 A. caviae는 1그룹만이 존재하였 다. A. veronii bv. sobria 의 4그룹에 대한 band 수는 2~4개까지 다양하게 나타났으며. 479~481 bp (ISR-1), 513~524 bp (ISR-2), 537~539 bp (ISR-3) 의 염기서열을 밝혀냈다. A. caviae는 3개의 band가 형성되었고,470~480bp (ISR-1), 521~525bp (ISR-2),568~602 bp (ISR-4)의 염기서열을 가지고 있었다. 그리고 이들에 대한 tRNA를 분석한 결과 ISR-1은 tRNAIle(GAT), tRNAAla(TGC)를 가지고 있고 ISR-2,3,4는 tRNAGlu(TTC)를 가지고 있었 다. A. caviae는 ISR-4의 151~281 bp에서 A. veronii bv. sobria 가 가지고 있지 않은 보존 적인 염기서열을 가지고 있었다. 그 중 A. vaviae의 178~197 bp 염기서열을 primer로 design하여 PCR을 실시한 결과 A. caviae 균주에서만 종특이적으로 생성되는 450 bp 정도 의 밴들르 얻을수 있었다.

• 한국미생물·생명공학회지
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• 제51권2호
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• pp.217-221
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• 2023

Aeromonas spp., are Gram-negative rods that can cause infections in healthy and immunocompromised hosts. The clinical presentation of gastroenteritis varies from mild diarrhoea to shigella-like dysentery to severe cholera-like watery diarrhoea. Here, we report a case of acute hemorrhagic gastroenteritis in a newborn infant by Aeromonas caviae and its draft genome sequence. It is important to reduce the chance of incorrect isolate identification, which could lead to the exclusion of pathogenic Aeromonas spp., from routine laboratory identification in cases of diarrheal diseases. The genome sequence of A. caviae SVJ23 represents a significant step forward in understanding the diversity and pathogenesis, virulence, and antimicrobial resistance profile.

• 한국식물병리학회지
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• 제11권1호
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• pp.47-52
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• 1995

One hundred and thirty bacterial isolates with high chitinolytic activity on chitin agar media were isolated and identified. Most of the isolates were Aeromonas hydrophila (110 isolates), and the others were Serratia marcescens (11 isolates), Aeromonas caviae (3 isolates), Chromobacterium violaceum strain C-61 (2 isolates), Chromobacterium violaceum strain C-72 (1 isolate) and unknown species (3 isolates). Among them, C. violaceum strain C-61 had highest chitinolytic activity and fungal growth inhibition on PDA. This bacterium also inhibited the growth of Rhizoctonia solani, Sclerotinia scelrotiorum, Phytophthora capsici and Pythium ultimum, but it didn't inhibit the growth of Fusarium oxysprum and Fusarium solani. C. violaceum strain C-61 suppressed damping-off of eggplant caused by R. solani. Populations of the chitinolytic bacteria such as Aeromonas hydrophila, Serratia marcescens, Aeromonas caviae, Chromobacterium violaceum strain C-61 and Chromobacterium violaceum strain C-72 introduced into R. solani-infested soil were continuously decreased until 20 days after treatment, but their populations except A. caviae were not changed significantly and maintained over 5$\times$104 CFU per g of soil thereafter.

• 환경위생공학
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• 제8권1호
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• pp.81-91
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• 1993

This paper was carried out to isolate and identify Aeromonas caviae which can degrade Sodium Dodecyl Benzene Sulfonate(SDBS) effectively. And the affecting factors for the ability of bacterial degradation were also studied. Frm October 1991 to February 1992, two hundred samples from sweage in Taegu area and Nakdong river waters in Talsung Gun area were tested. Minimal salt medium which contain SDBS only as a carbon source was used as a culture medium. The isolated new strain was identified as Aeromonas caviae Kim & Kweon. The optimal pH for SDBS degradation were 7.0 and temperature, $32^{\circ}C.$ It was taken 24 hours to degrade SDBS of 20mg/l completely under the optimal pH and temperature. And in the case of 30 mg/l of SDBS, it was taken 36 hours. The nitrogen sources were added to the minimal salt media containing 20mg/l of SDBS, and they were incubated at $32^{\circ}C$ for 14 hours. 86.9% SDBS were degraded after addition of 0.03% peptone as a organic nitrogen source. And 70.5% SDBS after addition of 0.05% ammonium sulfate as a inorganic nitrogen source. In the case of metal compounds(0.015%), the degradation rate for SDBS were 3.5 fold increased in the media containing magnesium chloride and calcium chloride than in the media that were not containing these metal compounds. And where the media containing magnesium chloride was 0.05%, the degradation rate was 65.8%. And above 0.3% NaCI, the degradation rate was decreased slowly.

• 한국미생물·생명공학회지
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• 제19권4호
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• pp.362-367
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• 1991

Pullulanase 생산균으로서 토양으로부터 분리한 Aeromonas caviae No.S-76을 진탕배양하여 얻은 조효소액을 ammonium sulfate 침전, DAEA Sephadex A-50 column chromatoraphy, Sephadex G-150 column chromatography에 의하여 정제하였다. 이때 수율은 21이었고 50배의 정제도를 가진 효소단백질을 얻었다. 정제효소는 SDS-polyacrylamide slab gel 정기영동에 의하여 분자량 118,000의 단일단백질이었고, 등전점은 4.3, 작용 최적온도는 $50^{\circ}C$, 작용 최적 pH는 8.0이었다. 또한 이효소는 $45^{\circ}C$ 이하, pH 6.0-90. 범위에서 안정성을 나타내었다. 이 효소를 $\beta$-cyclodextrin과 maltose의 고농도 혼합액에서 작용시켜 maltosyl-$\beta$-cyclodextrin을 합성하였다.

• 한국식물병리학회지
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• 제11권3호
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• pp.258-264
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• 1995

Production and some properties of chitinolytic enzymes were investigated by 80% ammonium sulfate precipitates (crude enzymes) from culture supernatant of antagonistic bacteria, Chromobacterium violaceum strain C-61 and strain C-72, Aeromonas hydrophila, Aeromonas caviae, and Serratia marcescens. The maximum production of chitinase was obtained from the 3-day culture at 28$^{\circ}C$ in C. violaceum stains, the 6-day culture in S. marcescens, and the 2-day culture in A. hydrophila and A. caviae. In the optimum culture periods, chitinase activity of C. violaceum strains C-61 was 1.5, 5.5, 12.0 and 11.3 times higher than those of strain C-72, S. marcescens, A. hydrophila and A. caviae, respectively. However, N,N'-diacetylchitobiase activity was 3.2 times higher in S. marcescens than in C. violaceum strain C-61, and that of Aeromonas spp.was very low. On gels containing glycol chitin, chitinase of C. violaceum strains showed four isoforms of 54-, 52-, 50- and 37-kDa, whereas there were four isoforms of 58-, 52-, 48- and 38-kDa in S. arcescens, three isoforms of 70-, 58- and 54-kDa in A. hydrophila and six isoforms of 90-, 79-, 71-, 63-, 58- and 38-kDa in A. caviae. The chitinase of C. violaceum strain C-61 was most active at pH 7.0 and at 5$0^{\circ}C$ and was stable in ranges of pH 5.0~10.0 for 2 hours and of 0~5$0^{\circ}C$ for 30 min.

• Journal of Microbiology
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• 제45권4호
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• pp.297-304
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• 2007

The detection of virulence factors of Aeromonas is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. In this study water samples were collected from a trout farm on a seasonal basis, and diseased fish and Aeromonas species were isolated and identified. For rapid detection of six virulence factors of isolated Aeromonas, a hexaplex-polymerase chain reaction (hexaplex-PCR) assay was used. The detected virulence factors include aerolysin (aer), GCAT (gcat), serine protease (ser), nuclease (nuc) lipase (lip) and lateral flagella (laf). The dominant strain found in our isolates was Aeromonas sobria, and the dominant virulence factors were aer and nuc for all seasons. We confirmed that A. sobria and two of the virulence genes (aer and nuc) are related. We proposed a method by which one can identify the major strains of Aeromonas: A. hydrophila, A. sobria, A. caviae, and A. veronii, using hexaplex-PCR.

• 미생물학회지
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• 제43권2호
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• pp.106-110
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• 2007

USEPA 1605 방법을 이용하여 서울시 상수도계통에서 Aeromonas를 조사하였다. 2002년 7월부터 2003년 12월까지 매월 한강수계 하천수와 서울시 정수장에서 공급되는 정수, 수돗물에서 시료를 채취하였다. Aeromonas는 선택배지(ADA-V)를 사용하여 membrane 필터 표면에 성장한 노란색 집락을 계수하여 측정하였다. 하천수에서는 Aeromonas가 $1.0{\times}10^{0}-9.8{\times}10^{3}\;CFU/ml$의 농도로 검출되었으며, 정수에서는 검출되지 않았다. 수돗물에서는 141개 중 1개의 시료에서 1 CFU/500 ml의 농도로 검출되었다. 확인된 Aeromonas는 대부분 비병원성인 A. salmonicida(51%)였고, 이 외에도 A. caviae(4.7%), A. schubertti(3.4%), A. sobria(3.8%), A. hydrophila(2.1%), A ichithiosmia(0.4%)등이 동정되었다. A. salmonicida에 대한 염소 저항성을 평가한 결과, 0.2 mg/L 염소농도에서 30초 접촉 후, 99.99% (일부)가 제거되었다. USEPA 1605 방법에서 제시한 정도관리를 수행한 결과, 정도관리 허용기준을 만족하였다. 본 연구를 통해 서울시 상수도계통에서 Aeromonas에 대한 안전성이 확보되었다.

• 대한수의사회지
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• 제19권1호
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• pp.33-38
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• 1983

Aeromonas punctata subgroup caviae and Plesiomonas shigelloides were isolated and identified from the lesion of ulcer disease of crusion carp in Ban Wol storing reservoir, the kyungki-do province.