DOI QR코드

DOI QR Code

Draft Genome Sequence of Aeromonas caviae Isolated from a Newborn with Acute Haemorrhagic Gastroenteritis

  • Savita Jadhav (Department of Microbiology, Symbiosis Medical College for Women (SMCW) & Symbiosis University Hospital and Research Centre (SUHRC), Symbiosis International (Deemed University)) ;
  • Ujjayni Saha (Symbiosis School of Biological Sciences (SSBS), Symbiosis International (Deemed University)) ;
  • Kunal Dixit (Symbiosis School of Biological Sciences (SSBS), Symbiosis International (Deemed University)) ;
  • Anjali Kher (Department of Paediatrics, Symbiosis Medical College for Women (SMCW) & Symbiosis University Hospital and Research Centre (SUHRC), Symbiosis International (Deemed University)) ;
  • Sourav Sen (University Research Department, Maharashtra University of Health Sciences) ;
  • Nitin Lingayat (Department of Paediatrics, Symbiosis Medical College for Women (SMCW) & Symbiosis University Hospital and Research Centre (SUHRC), Symbiosis International (Deemed University)) ;
  • Vivekanand Jadhav (Dr Naidu Infectious Diseases Hospital, Pune Municipal Corporation) ;
  • Sunil Saroj (Symbiosis School of Biological Sciences (SSBS), Symbiosis International (Deemed University))
  • Received : 2023.01.10
  • Accepted : 2023.04.25
  • Published : 2023.06.28

Abstract

Aeromonas spp., are Gram-negative rods that can cause infections in healthy and immunocompromised hosts. The clinical presentation of gastroenteritis varies from mild diarrhoea to shigella-like dysentery to severe cholera-like watery diarrhoea. Here, we report a case of acute hemorrhagic gastroenteritis in a newborn infant by Aeromonas caviae and its draft genome sequence. It is important to reduce the chance of incorrect isolate identification, which could lead to the exclusion of pathogenic Aeromonas spp., from routine laboratory identification in cases of diarrheal diseases. The genome sequence of A. caviae SVJ23 represents a significant step forward in understanding the diversity and pathogenesis, virulence, and antimicrobial resistance profile.

Keywords

Acknowledgement

We would like to thank Dr Rajiv Yeravdekar for their guidance and timely suggestions. We acknowledge the assistance provided by Mrs Varsha Shaikh at different stages of this study.

References

  1. Batra P, Mathur P, Misra MC. 2016. Aeromonas spp.: an emerging nosocomial pathogen. J. Lab. Physicians 8: 1-4. https://doi.org/10.4103/0974-2727.176234
  2. Hanninen ML, Siitonen A. 1995. Distribution of Aeromonas phenospecies and genospecies among strains isolated from water, foods or from human clinical samples. Epidemiol. Infect. 115: 39-50. https://doi.org/10.1017/S0950268800058106
  3. Giannattasio-Ferraz S, Maskeri L, Oliveira AP, Barbosa-Stancioli EF, Putonti C. 2020. Draft genome sequence of Aeromonas caviae UFMG-H8, isolated from urine from a healthy bovine heifer (Gyr breed). Microbiol. Resour. Announc. 9: e00388-20.
  4. Nagar V, Shashidhar R, Bandekar JR. 2013. Characterization of Aeromonas strains isolated from Indian foods using rpoD gene sequencing and whole cell protein analysis. World J. Microbiol. Biotechnol. 29: 745-752. https://doi.org/10.1007/s11274-012-1212-1
  5. Beatson SA, das Gracas de Luna M, Bachmann NL, Alikhan NF, Hanks KR, et al. 2011. Genome sequence of the emerging pathogen Aeromonas caviae. J. Bacteriol. 193: 1286-1287. https://doi.org/10.1128/JB.01337-10
  6. Nowrotek M, Jalowiecki L, Plaza G. 2021. Fluoroquinolone resistance and virulence properties among wastewater Aeromonas caviae isolates. Microb. Drug Resist. 27: 179-189. https://doi.org/10.1089/mdr.2019.0287
  7. Vila J, Marco F, Soler L, Chacon M, Figueras MJ. 2002. In vitro antimicrobial susceptibility of clinical isolates of Aeromonas caviae, Aeromonas hydrophila and Aeromonas veronii biotype sobria. J. Antimicrob. Chemother. 49: 701-702. https://doi.org/10.1093/jac/49.4.701
  8. Ehrt S, Schnappinger D. 2003. Isolation of plasmids from E. coli by alkaline lysis. Methods Mol. Biol. 235: 75-78. https://doi.org/10.1385/1-59259-409-3:75
  9. Gurevich A, Saveliev V, Vyahhi N, Tesler G. 2013. QUAST: quality assessment tool for genome assemblies. Bioinformatics 29: 1072-1075. https://doi.org/10.1093/bioinformatics/btt086
  10. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. 2015. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res. 25: 1043-1055. https://doi.org/10.1101/gr.186072.114
  11. Wattam AR, Abraham D, Dalay O, Disz TL, Driscoll T, Gabbard JL, et al. 2014. PATRIC, the bacterial bioinformatics database and analysis resource. Nucleic Acids Res. 42: 581-591.
  12. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP, Zaslavsky L, et al. 2016. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res. 44: 6614-6624. https://doi.org/10.1093/nar/gkw569