• Journal of Microbiology and Biotechnology
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• v.33 no.11
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• pp.1531-1541
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• 2023

Lipase is a well-known and highly in-demand enzyme. During the last decade, several lipase optimization studies have been reported. However, production costs have always been a bottleneck for commercial-scale microbial enzyme production. This research aimed to optimize the conditions for lipase production by Limtongozyma siamensis DMKU-WBL1-3 via a One-Factor-At-a-Time (OFAT) approach combined with statistical methods while using a low-cost substrate. Results suggest that low-cost substrates can be substituted for all media components. An optimal medium was found, using response surface methodology (RSM) and central composite design (CCD), to consist of 0.50% (w/v) sweet whey, 0.40% (w/v) yeast extract (food grade), and 2.50% (v/v) palm oil with the medium pH adjusted to 4 under shaking flask cultivation. From an economic point of view, this work was successful in reducing production costs while increasing lipase productivity. The medium costs were reduced by 87.5% of the original cost while lipase activity was increased by nearly 6-fold. Moreover, lipase production was further studied in a 2-L stirred-tank fermentor. Its activity was 1,055.6 ± 0.0 U/ml when aeration and agitation rates were adjusted to 1 vvm and 170 rpm, respectively. Interestingly, under this optimal lipase production, the yeast showed accumulated lipids inside the cells. The primary fatty acid is a monounsaturated fatty acid (MUFA) that is typically linked to health benefits. This study hence reveals promising lipase production and lipid accumulation by L. siamensis DMKU-WBL1-3 that are worthy of further study.

• Korean Journal of Health Education and Promotion
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• v.29 no.5
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• pp.37-48
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• 2012

Objectives: The economic evaluation of health promotion programs has increasingly become an imperative activity for securing public fund or budget. The purpose of this study is to conduct an economic evaluation for the healthy school canteen program using the contingent valuation method(CVM). Methods: To estimate the benefit from the healthy school canteen program, double-bounded dichotomous choice method as a sort of willingness to pay was employed. Four hundred parents who lived in Seoul and have students at middle or high schools, were administrated by semi-constructive questionnaire containing the necessary information for benefit estimation. Cost estimation of healthy school canteen program was made referring to three types of pilot programs. Finally, the benefit against the cost was worked out according to the three levels of estimated cost. Results: Cost estimate is 8,488 and 9,311 won depending on the two senarios about how to invest on the program, respectably. The results of benefit estimate shows that the average cost willingness to pay(WTP) for healthy school canteen program is 21,275 won(16,963-59,838 won, 95% CI) and total benefits turned out 14.7 billion won. According to the cost-benefit analysis, average benefit-cost ratio is from 2.3 to 3.6. Conclusions: Healthy school canteen program could be economically accepted, and government can take consideration of expanding beneficiaries of healthy school canteen program for health promotion.

• Journal of the Korean Professional Engineers Association
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• v.14 no.2
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• pp.5-11
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• 1981

This analysis has been conducted for 8 chemical plants built in Korea during 1975-1976 and the results have been compared with those in U.S.A. observed by Kenneth M. Guthrie. Equipment and materials supplied by domestic makers are limited in activity field and occupy only 12% of all equipment and material cost. However, unit costs of most materials supplied by home such as cement, steel, cables and carbon steel pipes, etc. being relatively cheaper than those of abroad, their effects on material-to-equipment ratios are apparent. There is a deep difference in labor wage rates between Korea and U.S.A. as much as 16 times. However, the labor productivity observed in these model projects is in the range of 1.1-1.4 to the Gulf Coast, U.S.A. during the same period. And this low wage rate and relatively high productivity have the greatest influence on deviation of Korean cost structure from that of U.S.A. In these model projects, engineering and construction management was conducted by foreign contractors (mostly U.S.A.), and the resulting project indirect cost amounts to 7.5 times as high as most cases executed by Korean contractors alone. Weighted percentage of each component and L/M-ratio by activities of direct field cost derived from this analysis are shown in detail in Tables 2 and 5 to provide a basis for futher study and comparison.

• Journal of the Korea Society of Computer and Information
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• v.5 no.4
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• pp.200-210
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• 2000

Distribution Activity for customer is preferred at Physical distribution system in e-commerce, so fast delivery and low distribution cost determine the core competency of enterprise. Because there is no cooperative system between with distribution centers in traditional distribution system, it is difficult to optimize the system. The purpose of this paper is to propose a cost structure model for multi-stage distribution system, which is used for determination of joint lot size and optimal demand and transportation policy. It is expected to contribute to development of algorithm for joint distribution system, which is minimized the transportation cost by the result of this cost structure model.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.2
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• pp.231-237
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• 2006

A controversy get more deeply intensified about the price of inpatient's meal provided in hospital as the Ministry of Health and Welfare declared the National Health Insurance will cover inpatient's meal from 2006. By using newly designed cost analysis method and stratification method of the population, the optimal price of inpatient's meal are derived based on the 71 sample hospitals. This study analyzed 71 samples based on the activity based costing(ABC) and the method of relative units value(RUV). The factors influencing the level of cost are found by linking the results of cost and statistical analysis. As the key factors influencing the cost are the number of employees in a nutrition department and a the number of meals provided to the patients, These factors should be considered when the optimal price of inpatient meals is set for the coverage of the National Health Insurance.

• Journal of Korean Society for Quality Management
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• v.39 no.1
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• pp.109-119
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• 2011

This study distinguished innovation activities into 3 main things (sustainability management activity, management innovation activity and quality innovation activity). Performance differences among those 3 innovation activities are again distinguished into 4 things (affecting on sales revenue, increasing productivity, improving its corporate image and cost reduction) to demonstrate recognition of performance. We also demonstrated difference of performance between manager and other workers.

• Journal of Environmental Science International
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• v.26 no.2
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• pp.239-248
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• 2017

Green synthesis of gold nanoparticles(GNPs) considered more ecofriendly and cost effective than other chemical methods use of dangerous reagents and solvents, improved material and energy efficiency and enhanced design of non-toxic products. In this study, we developed a green synthesis method for using Caulis in Taeniam (BCT). BCT were characterized by UV-vis, Zetasizer, TEM, XRD, and FTIR. The antioxidant activity of BCT was determined by DPPH and ABTS radical-scavenging assays, and heme oxygenase-1 induction in RAW 264.7 macrophages. The resulting BCT appeared spherical with an average diameter of 67.171.39 nm The aAntioxidant activity was increased in a dependent manner. To conclude, the green synthesis of BCT-GNPs was successful, and it appers to be useful in the for future applications.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.6 no.9
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• pp.1-5
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• 1983

This study takes over the application of survey sampling theory to activity sampling and the application of activity sampling to time standard estimation. Cluster, stratified, and multistage sampling are studied in conjunction with random and systematic sampling. Estimation procedures that will maximize the information obtained per cost expended on the study and specification of the procedure to be used to estimate the accuracy of the estimates for the adopted procedure are considered. The use of multiple regression md linear programming to estimate standard element performance time from typical job lot production data is also considered.

• Journal of the Korean Chemical Society
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• v.66 no.3
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• pp.194-201
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• 2022

A simple, efficient, and cost-effective method has been employed for the synthesis of 2,4,5-trisubstituted imidazole derivatives (3a-j) containing quinoline substituent at 2^nd position. Title compounds were obtained by multicomponent reaction (MCR), involving aryl substituted 1,2-diketone, quinoline carbaldehyde and ammonium acetate in the presence of acetic acid solvent under mild reaction conditions. The newly synthesized quinoline containing imidazole derivatives were confirmed through FT-IR, ¹H-NMR, ¹³C-NMR and mass spectral analysis. In-vitro microplate alamar blue assay (MABA) to determine the MIC (minimum inhibitory concentration) values against Mycobacterium tuberculosis H37Rv was performed for the synthesized compounds. The synthesized compounds exhibited activity against Mycobacterium tuberculosis and among which compounds, 3d, 3f and 3i showed good activity. The highest activity was showed with compound 3i. The anti-mycobacterial activity results are well correlated with the computational molecular docking analysis, which was performed for the synthesized compounds prior to the evaluation of the activity.

• Industrial Engineering and Management Systems
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• v.11 no.2
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• pp.183-187
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• 2012

In this paper, we study the fixed charge transportation problem with uncertain variables. The fixed charge transportation problem has two kinds of costs: direct cost and fixed charge. The direct cost is the cost associated with each source-destination pair, and the fixed charge occurs when the transportation activity takes place in the corresponding source-destination pair. The uncertain fixed charge transportation problem is modeled on the basis of uncertainty theory. According to inverse uncertainty distribution, the model can be transformed into a deterministic form. Finally, in order to solve the uncertain fixed charge transportation problem, a numerical example is given to show the application of the model and algorithm.