• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2095-2105
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• 2018

In our previous studies, we have identified several in vivo-induced antigens and evaluated their potential as subunit vaccine candidates in a murine model, in which the recombinant protein GalT showed the most potent immunogenicity and immunoprotective efficacy against Actinobacillus pleuropneumoniae. To exploit a more efficient way of delivering GalT proteins, in this study, we employed the widely studied E. coli outer membrane vesicles (OMVs) as a platform to deliver GalT protein and performed the vaccine trial using the recombinant GalT-OMVs in the murine model. Results revealed that GalT-OMVs could elicit a highly-specific, IgG antibody titer that was comparable with the adjuvant GalT group. Significantly higher lymphocyte proliferation and cytokines secretion levels were observed in the GalT-OMVs group. 87.5% and 50% of mice were protected from a lethal dose challenge using A. pleuropneumoniae in active or passive immunization, respectively. Histopathologic and immunohistochemical analyses showed remarkably reduced pathological changes and infiltration of neutrophils in the lungs of mice immunized with GalT-OMVs after the challenge. Taken together, these findings confirm that OMVs can be used as a platform to deliver GalT protein and enhance its immunogenicity to induce both humoral and cellular immune responses in mice.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.21 no.1
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• pp.19-26
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• 2021

The purpose of this study is to provide an environment that can support the development of the virtual asset industry. The limiting factors and countermeasures currently possessed by the virtual asset industry were considered in terms of legal and institutional aspects, technical aspects, and market aspects.Small businesses classified as virtual asset operators have difficulty meeting the government's requirements.Accordingly, SMEs with insufficient funds and manpower are withdrawn from the market, creating an environment where only large-scale enterprises with capital power survive.It is difficult to develop desirable technologies and markets in the virtual asset technology industry. In addition, small and medium-sized companies may be expelled from the market, causing damage to current users. Therefore, in terms of legal and institutional aspects, there is a lack of an exact scope of virtual asset providers, and thus it is necessary to respond to the controversial elements of virtual asset providers. In terms of technology, it is necessary to cope with the slowdown of the P2P method, the difficulty in recovering errors, and the absence of operational experts. Therefore, technology standardization and stabilization are required, and efforts must be made to cultivate operational technical personnel who can support them.In terms of the market, it is necessary to prepare measures to protect users of virtual assets and to establish countermeasures for companies operating virtual assets against weak user protection, inadequate application of the AML method, and limitations of taxation. This study is expected to contribute to active utilization support or related policies in the virtual asset industry.

• Journal of Digital Convergence
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• v.19 no.2
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• pp.1-12
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• 2021

With the recent 4th Industrial Revolution, the growth and value of big data are continuously increasing, and the government is also actively making efforts to open and utilize public data. However, the situation still does not reach the level of demand for public data use by citizens, At this point, it is necessary to identify research trends in the public data field and seek directions for development. In this study, in order to understand the research trends related to public data, the analysis was performed using topic modeling, which is mainly used in text mining techniques. To this end, we collected papers containing keywords of 'Public data' among domestic and foreign research papers (1,437 domestically, 9,607 overseas) and performed topic modeling based on the LDA algorithm, and compared domestic and foreign public data research trends. After analysis, policy implications were presented. Looking at the time series by topic, research in the fields of 'personal information protection', 'public data management', and 'urban environment' has increased in Korea. Overseas, it was confirmed that research in the fields of 'urban policy', 'cell biology', 'deep learning', and 'cloud·security' is active.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.24 no.1
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• pp.1-14
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• 2021

The Ministry of Environment of Korea has been releasing Asiatic black bears since 2004 at Jirisan National Park. It exceeded the target number of Asiatic black bears in 2018. As of July 2020, in addition to 67 traceable bears, many Asiatic black bears are dispersed outside Jirisan National Park. Jirisan National Park is a very dense place with more than 3 million visitors every year. In this study, the roads and trails through Jirisan National Park were considered to be the main dispersion factors of Asiatic black bears, and the fragmentation analysis was conducted. As of July 2017, the length of roads and trails in Jirisan National Park was 363.4km. Based on this, Jirisan National Park was fragmented into 163 patches. There is only one place that maintains a single area of more than 50㎢ that is suitable for large mammals to inhabit, and 141 places are less than 5㎢. There are 6 patches of 24 to 200㎢ area suitable for living of large mammals including Asiatic black bears, in Jirisan National Park. Compared to the announcement made by the Korea National Park Service in 2014, activities of Asiatic black bears were rare in areas below 5㎢ area and the frequency of activities was high in more than 20㎢ area. This shows that human activities in protected areas such as national parks can directly affect the activities of wild animals, including Asiatic black bears. Therefore, efforts should be made to improve the habitat of Asiatic black bears by reducing the pressure of visiting for Jirisan National Park. In addition, as a result of analyzing the fragmentation of the park's natural conservation zone 107.7km, 45% of the trail of Jirisan National Park, was opened, which is more fragmentation than other use zones. The park nature conservation zone accounts for about 32% of the total area of Jirisan National Park, but the average patch area is only 2.93㎢ and seven large shelters are located. Therefore the Asiatic black bears are negatively affected. This is the result of inconsistent national park use zone setting and actual park management. In overseas countries, research is active on the negative effects of human activity on ecosystems in protected areas. However, there is a lack of research of that in Korea. Thus, that research is required for protection area management in the future.

• Informatization Policy
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• v.28 no.1
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• pp.3-21
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• 2021

Klaus Schwab's discussion on the Fourth Industrial Revolution provides a framework for predicting the direction of legal technology development. Technological convergence, which has emerged as the core concept of the Fourth Industrial Revolution has a significant effect on legal technology. In particular, various new technologies, such as legal chatbots and platforms, are being introduced to enhance efficiency and accessibility in the legal field. However, legal technology is still in its early stage, with institutional improvement needed to vitalize the industry. In this paper, we first specify the concept and classification of legal technology in Chapter 2, followed by trends and limitations in Chapter 3 and ways of vitalizing legal technology in the future in Chapter 4. To invigorate legal technology development, it is necessary to put in place legal regulatory measures that stipulate the active disclosure of legal data, such as precedents, and make free use of such measures. In the law, many issues, such as the safety of artificial intelligence, personal information protection, and ethical standards, will be discussed in the future. Therefore, via this paper, we hope to promote the formation of social consensus and prepare countermeasures, such as legislative measures.

• Journal of the Korean Association of Geographic Information Studies
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• v.25 no.4
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• pp.222-237
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• 2022

This study was performed to predict change of forestland area in future to 2050 based on System Dynamics Model which is based on feedback loop by causal relationship. As forestland area change in the future depends on potential forestland conversion demands, each demand type of forestland conversion such as agricultural, industrial, public and residential/commercial use was modeled using annual GDP, population, number of household, household construction permission area (1981~2019). In results, all of conversion demands would have continuously decreased to 2050 while residential and commercial land would be reduced from 2034. Due to such shortage, eventually, total of forestland in South Korea would have decreased to 6.18 million ha when compared to current 6.29 million ha. Moreover, the forestland conversion to other use types must be occurred continuously in future because most of forestland is owned privately in South Korea. Such steady decrement of forestland area in future can contribute to the shortage of carbon sink and encumber achievement of national carbon-neutral goal to 2050. If forestland conversion would be occurred inevitably in future according to such change trends of all types, improved laws and polices related to forestland should be prepared for planned use and rational conservation in terms of whole territory management. Therefore, it is needed to offer sufficient incentive, such as tax reduction and payment of ecosystem service on excellent forestland protection and maintenance, to private owners for minimizing forestland conversion. Moreover, active afforestation policy and practice have to be implemented on idle land for reaching national goal 'Carbon Neutral to 2050' in South Korea.

• Korean Journal of Heritage: History & Science
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• v.55 no.2
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• pp.232-249
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• 2022

The purpose of this article is to review the status of designation and preservation, management of national prehistoric sites and to suggest future applications. Prior to the discussion, the overall status of national prehistoric sites was reviewed. Specifically, the current status of designation, designated area, and distribution by region of national prehistoric sites according to the period and nature of the sites were examined in detail. Next, in order to understand the preservation and management of national prehistoric sites, I report the results of field trips to several sites, such as Geomdan-ri in Ulju, Songguk-ri in Buyeo, and Seoktaek-ri in Hongseong, and propose some measures for preservation, maintenance, and management of the remains. Finally, by referring to cases of US national historic sites such as protection, conservation, operation and management system and academic research project of cultural heritage, it is necessary to consider the need for a direction of the utilization that considers both socio-economic aspects as well as education and research on cultural heritage. The active use of cultural heritage can be a measure that leads to the continuous preservation and management of the sites, and ultimately will be a shortcut to increasing the value of national prehistoric sites.

• Journal of Digital Convergence
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• v.20 no.5
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• pp.261-268
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• 2022

This study tried to understand the meaning of resource attraction, entertainment experience, love mark, and attachment of tourist destinations. A theoretical study was conducted on each research concept to achieve the proposed research purpose. Based on this, an empirical study was conducted after deriving a questionnaire based on this, and a non-face-to-face survey was conducted nationwide using population-proportional sampling. The empirical study was confirmed through the statistical programs SPSS25.0 and AMOS25.0. The resource attractiveness of the tourist destination had a positive (+) relationship with the influence on the tourists' experience. The relationship between the experience and the love mark had a positive (+) relationship. A positive (+) relationship was also formed in the relationship between experience and attachment. However, the relationship between love mark and attachment did not form a significant relationship.Because it reflects regional characteristics, it is a useful material for local culture and history, so protection, preservation, and active program development are required. is needed.

• Journal of Science Education
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• v.47 no.2
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• pp.139-153
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• 2023

This study aimed to investigate the effect of environmental education programs using action learning on elementary school students' environmental literacy and self-efficacy. To this end, an environmental education program using action learning was applied to 22 sixth graders of 'A' Elementary School in Busan. The results of this study were as follows. First, environmental education programs using action learning were effective in improving the environmental literacy of elementary school students. After implementing the environmental education program using action learning, students' environmental values, cognitive, and procedural ability changed positively. Environmental issues awareness and ecological knowledge improved, and there was a significant increase in environmental behavior and environmental function. Second, environmental education programs using action learning were effective in enhancing the self-efficacy of elementary school students. After implementing environmental education programs using action learning, students' confidence in problem solving and their ability to judge their behaviors improved. Third, elementary school students showed active participation and interest when conducting environmental education programs using action learning. Students have become open to protecting the environment when they find problems, devise solutions, and practice them. After reflection, it was confirmed that students' immersion and satisfaction in class were high as a solution was reached, leading to efforts to sell items they did not use and donate profits to environmental protection organizations.

• The Korean Journal of Franchise Management
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• v.8 no.2
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• pp.25-36
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• 2017

Purpose - Recently, entrepreneurship education has been revitalized with interest in entrepreneurship. Entrepreneurship education is an educational service activity that is provided for entrepreneurship and individual start-up success within a certain period of time. According to previous studies on entrepreneurship and entrepreneurship, the satisfaction of entrepreneurship education affects entrepreneurship and as a result increases entrepreneurship. In recent years, the number of female entrepreneurs has also increased as the number of entrepreneurial issues has increased. Based on previous studies, this research proposed the theoretical framework about the structural relationships among mentoring quality (career development, psychological social, role modeling), education satisfaction, recommendation intention and entrepreneurial intention. This study is to find out the possibility of attempting to create a theoretical basis for entrepreneurial mentoring education in entrepreneurship education program. Research design, data, and methodology - In this model, mentoring quality consists of three sub-dimensions such as career development, psychological social, and role modeling. In order to test research model and hypotheses, the data were collected from 203 female college students who participated in entrepreneurial education. The data were analyzed using frequency analysis, confirmatory factor analysis, correlation analysis, and structural equational modeling with SPSS 24.0 and SmartPLS 3.0 statistical program. Result - The results of the study are as follows. First, role modeling has a positive effect on recommendation intention and entrepreneurial intention. Second, career development has a strong negative effect on the entrepreneurial intention. Third, career development and role modeling had a positive effect on educational satisfaction, and educational satisfaction had positive influence on recommendation intention and entrepreneurial intention. Conclusions - As women's social advancement becomes more active, start-up support programs including entrepreneurship mentoring are increasing. The results of this study suggest how to use the mentoring program mix and how to allocate the resources for the education program when the entrepreneurial education manager plans and executes the mentoring education program. For example, this study shows that career development and role modeling enhance educational satisfaction, and in turn increase recommendation intention and entrepreneurial intention. This means that entrepreneurship education should consist of contents that include career development functions such as sponsorship, guidance, protection, and provision of challenging work. In addition, the findings of this study indicate that mentors should perform the function of allowing the participants to have confidence and professional thinking ability at the time of start up based on their experiences.