• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.38-38
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• 2019

Among the 6500 accessions of the pepper germplasm which conserved at RDA Genebank, 319 accessions were investigated charateristics such as their growth habit, flowering and fruit shape in Capsicum frutescens. All accessions were characterized for their 14 qualitative and 16 quantitative characters. Results revealed that both qualitative and quantitative characters exhibited wide variation among the studied germplasm. The hypocotyl color, which investigated in juvenile stage, was divided in three types such as green, purple, and purple with green, and most of the accessions showed in purple. Flowering starts from 7 May, and most accessions were starting to flower from 27 May to 25 June. The fresh color of the mature pepper were red, yellow, orange, Etc. Fruit shape were mostly elongated, while exhibited ball shape and round shape were a few. The fresh weight of fruits ranged from 0.2 to 42.3, and the diameter of fruit showed from 4.2 to 45.1 mm, respectively. The phenotypic diversity exists in Capsicum frutescens. provide valuable information to improve agronomic traits in pepper breeding program.

• 한국산림과학회지
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• 제109권4호
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• pp.421-428
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• 2020

Tamarind (Tamarindus indica L.) is one of the multipurpose tree species distributed in the tropical and sub-tropical climates. It is an important fruit yielding tree that supports the livelihood and has high social and cultural values for rural communities. The vegetative, reproductive, qualitative, and quantitative traits of tamarind vary widely. Characterization of phenotypic and genetic structure is essential for the selection of suitable accessions for sustainable cultivation and conservation. This study aimedto examine the genetic relationship among the collected accessions of sweet, red, and sour tamarind by using Random Amplified Polymorphic DNA (RAPD) primers. Nine accessions were collected from germplasm gene banks and subjected to marker analysis. Fifteen highly polymorphic primers generated a total of 169 fragments, out of which 138 bands were polymorphic. The polymorphic information content of RAPD markers varied from 0.10 to 0.44, and the Jaccard's similarity coefficient values ranged from 0.37 to 0.70. The genetic clustering showed a sizable genetic variation in the tamarind accessions at the molecular level. The molecular and biochemical variations in the selected accessions are very important for developing varieties with high sugar, anthocyanin, and acidity traits in the ongoing tamarind improvement program.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.211-211
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• 2022

Amaranth is a nutritious and broadly adapted seed crop in high demand around the world. A preliminary approach for understanding the genetics of amaranth resources entails a morphologic characterization, which can provide the basis for breeding the first variety in Korea, leading to satisfying the needs of farmers and consumers. Therefore, this study aimed to evaluate the phenotypic characteristics of ten genetic amaranth accessions for the selection of outstanding accessions in terms of yield and grain quality. A randomized complete block design was used, with fifteen replications for each accession under field conditions. Five quantitative and three qualitative descriptors were evaluated with descriptive analysis. The results showed that the accessions with plant heights smaller than the average (>112.7 cm) presented lower yields and smaller seed sizes, thus decreasing the grain quality. The cluster analyses established groups of accessions with good yields (>30.1 g of seeds per plant) and stable morphological characteristics. Based on yield and morphological descriptors, the proposed selection index indicated four accessions as potential parents for amaranth breeding programs in Kora.

• 한국자원식물학회지
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• 제34권6호
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• pp.600-607
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• 2021

Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).

• 한국약용작물학회지
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• 제19권5호
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• pp.347-353
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• 2011

In this study, simple sequence repeat (SSR) analyses were utilized for evaluation of genetic diversity and discrimination of 17 accessions. Five cultivars, which were developed from Korea, and 12 foreign accessions, which were collected from China, Japan, Russia and USA, were evaluated by nine markers out of 22 SSR markers. A total of 39 alleles were detected, ranging from 2 to 8, with an average of 4.3 alleles per locus. The expected heterozygosity and PIC values were 0.627 and 0.553, with a range from 0.21 (GB-PG-078) to 0.76 (GB-PG-142) and from 0.19 (GB-PG-078) to 0.70 (GB-PG-142), respectively. Four makers out of nine SSR markers, GB-PG-026, GB-PG-043, GB-PG-142 and GB-PG-177, were selected as key factors for discrimination of Korean ginseng cultivars and foreign accessions. All of Korean ginseng cultivars and foreign accessions were individually by the combination of four SSR markers. Consequently, the SSR markers developed in this study may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and foreign accessions.

• 한국약용작물학회지
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• 제26권1호
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• pp.32-41
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• 2018

Background: To obtain useful safflower resources for breeding and research of functional materials, the present study was conducted to determine fatty acid compositions and antioxidant activities of 281 safflower accessions collected from East Asia including South Korea. Methods and Results: Lipid contents and fatty acids compositions were evaluated using soxhlet extraction and gas chromatography, respectively. A antioxidant activities were analyzed using a spectrophotometer. The evaluation range of safflower accessions showed very wide variation. In terms of lipid contents, the China accessions were higher than the collection from other regions, whereas antioxidant activities did not differ among regions. The result of the principal component analysis showed that the first and second principal component cumulatively explained 90.6% of the total variation. In clustering the safflower accessions, the tree showed four major clades. Group II (16 accessions) was high in lipid content, oleic acid and linoleic acid, whereas group III (50 accessions) exhibited higher antioxidant activities than other groups. Conclusions: It was recommended that the China collections be utilized as a useful resource for research on functional oil materials. These results provided valuable information for safflower breeders and researchers of functional food.

• The Plant Pathology Journal
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• 제32권1호
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• pp.58-64
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• 2016

Bacterial wilt of tomatoes caused by Ralstonia solanacearum is a devastating disease that limits the production of tomato in Korea. The best way to control this disease is using genetically resistant tomato plant. The resistance degree to R. solanacearum was evaluated for 285 tomato accessions conserved in the National Agrobiodiversity Center of Rural Development Administration. These accessions of tomato were originated from 23 countries. Disease severity of tomato accessions was investigated from 7 days to 14 days at an interval of 7 days after inoculation of R. solanacearum under greenhouse conditions. A total of 279 accessions of tomato germplasm were susceptible to R. solanacearum, resulting in wilt and death in 70 to 90% of these plants. Two tomato accessions were moderately resistant to R. solanacearum. Only four accessions showed high resistance against R. solanacearum. No distinct symptom of bacterial wilt appeared on the resistant tomato germplasms for up to 14 days after inoculation of R. solanacearum. Microscopy of resistant tomato stems infected with R. solanacearum revealed limited bacterial spread with thickening of pit membrane and gum production. Therefore, these four resistant tomato germplasms could be used in tomato breeding program against bacterial wilt.

• 한국자원식물학회지
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• 제15권1호
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• pp.36-42
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• 2002

전북, 경남, 강원, 경기지역에서 수집한 16개의 수집종 삽주의 정량적 특성을 비교하고, 다변량 분석법에 의하여 수집종 삽주의 유연관계를 분석한 결과를 요약하면 다음과 같다. 함양1 수집종이 초장 86.2cm, 주당잎수 117.6개, 화경수 34.3개로 가장 좋았고, 수도1 수집종이 경수가 12개로 가장 많았다. 또한, 엽연거치는 평균 1.6mm로 모두 존재하였다. 삽주속 식물의 잎의 형태는 단엽과 장상복엽(3출엽)이 주로 존재하였고, 엽형은 타원형이 가장 많았다. 주요 18개의 형질을 대상으로 다변량 분석법에 의한 16개 수집종 삽주는 3개의 계통군으로 분류할 수 있었으며 제 1군에 속하는 진안1, 진안2 수집종과 제 3군에 속하는 정수, 거제 수집종이 유연관계가 가장 먼 것으로 나타났다.

• 한국약용작물학회지
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• 제18권2호
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• pp.86-92
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• 2010

The polymorphism and the genetic relationships among 32 genetic resources of genus Nelumbo from Korea, Japan, China, USA, India, Thailand and Gabong were thoroughly investigated and extensively examined using ISSR markers. Out of 103 loci detected overall, 94 were identified to be polymorphic with a rate of 91.2%. The genetic similarity matrix revealed a wide range of variability among the 32 accessions, spanning from 0.227 to 0.833. The study findings indicate that the Nelumbo accessions have a high genetic diversity, and accordingly carry a germplasm qualifying as good genetic resources for cross breeding. According to the clustering analysis, different subspecies, N. nucifera and N. lutea, were divided into independent groups and all of the N. nucifera accessions could be classified into five categories. Compared to RAPD analysis, ISSR method showed a clearer picture of polymorphism among the accessions and exhibited a definite distinction even among the subspecies. In this respect, ISSR analysis is considered to be more effective in differentiating the accessions and subspecies of the genus Nelumbo than RAPD test.

• 한국작물학회지
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• 제56권2호
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• pp.167-176
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• 2011

Brown planthopper (BPH) is a serious insect pest of rice crop throughout rice growing countries, and yield loss due to its infection can be up to 60%. This study aimed to evaluate efficiency of molecular markers for screening BPH resistance accessions among 86 aromatic rice germplasm Eighty-six accessions of aromatic rice germplasm included two accessions of Tongil type (bred in Korea), 28 accessions of japonica type and 56 accessions of indica type. We applied eight STS markers (pBPH9, pBPH19, pBPH20, pBPH21, AJ09-b, RG457L, RG457B, and 7312.T4A) which were linked to four of BPH resistance genes, Bph1, Bph13(t), Bph10, and Bph18(t) respectively. One japonica type accession, 415XIr352, and six indica type accessions possessed one or four positive bands when tested with four STS markers linked to Bph1 gene. One indica type aromatic rice, Basmati9-93, showed the target bands linked to the Bph10 gene. The other accessions did not show same fragments as the respective resistant lines. Bph13(t) is the most widely introduced resistance gene and only one accession showed positive bands implying that this accession might harbor Bph10 and Bph18(t) genes. Three aromatic accessions, Domsiah, Khao Dawk Mali 105 and 415XIr352 showed gene pyramiding of Bph1 and Bph13(t). Two indica aromatic rice, Ds 20 and Basmati 9-93, possessed at least two BPH resistance genes, Bph1, Bph18(t) and Bph13(t), Bph18(t), respectively. These results indicates that aromatic rice germplasm have narrow diversities of BPR resistance genes.