Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Analysis of Genetic Polymorphism of Korean Ginseng Cultivars and Foreign Accessions using SSR Markers

SSR 마커를 이용한 국내산 인삼 품종 및 국외 수집종의 유전적 다양성 분석

  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 조익현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 정종욱 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이제완 (산림청 국립산림과학원 대외협력과) ;
  • 서아연 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박종현 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 김옥태 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 현동윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2011.09.08
  • Accepted : 2011.10.08
  • Published : 2011.10.30
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Abstract

In this study, simple sequence repeat (SSR) analyses were utilized for evaluation of genetic diversity and discrimination of 17 accessions. Five cultivars, which were developed from Korea, and 12 foreign accessions, which were collected from China, Japan, Russia and USA, were evaluated by nine markers out of 22 SSR markers. A total of 39 alleles were detected, ranging from 2 to 8, with an average of 4.3 alleles per locus. The expected heterozygosity and PIC values were 0.627 and 0.553, with a range from 0.21 (GB-PG-078) to 0.76 (GB-PG-142) and from 0.19 (GB-PG-078) to 0.70 (GB-PG-142), respectively. Four makers out of nine SSR markers, GB-PG-026, GB-PG-043, GB-PG-142 and GB-PG-177, were selected as key factors for discrimination of Korean ginseng cultivars and foreign accessions. All of Korean ginseng cultivars and foreign accessions were individually by the combination of four SSR markers. Consequently, the SSR markers developed in this study may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and foreign accessions.

Keywords

References

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