Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant Activities of Rehmannia glutinosa by Traditional Methods

생지황을 이용하여 전통방법으로 제조한 숙지황의 항산화 활성

  • Kim, Hyo-Jin (Department of Food & Nutrition, Chungnam National University) ;
  • Lee, Ji-Yeon (Department of Food & Nutrition, Chungnam National University) ;
  • You, Bo-Ram (Department of Food & Nutrition, Chungnam National University) ;
  • Doh, Eun-Soo (Department of Oriental Pharmaceutical Science, Joongbu University) ;
  • Kim, Mee-Ree (Department of Food & Nutrition, Chungnam National University)
  • 김효진 (충남대학교 식품영양학과) ;
  • 이지연 (충남대학교 식품영양학과) ;
  • 유보람 (충남대학교 식품영양학과) ;
  • 도은수 (중부대학교 한방제약과학과) ;
  • 김미리 (충남대학교 식품영양학과)
  • Received : 2011.09.14
  • Accepted : 2011.10.05
  • Published : 2011.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of this study was to evaluate antioxidant activities of Rehmannia glutinosa (Raw Jihwang) by traditional method. The total phenol content of Rehmannia radix Preparata (the final cycle of Jihwang) was increased to 205%, compared with Rehmannia glutinosa. Antioxidant activities, determined by ferric-reducing antioxidant potential (FRAP), 2,2'-azinobis(3 ethybenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, 1,1-diphenyl-2-picrydrazyl (DPPH) and hydroxyl radical scavenging activities, increased remarkably as the number of steaming-drying cycles increased. Especially, FRAP value increased 285%. Also, $IC_{50}$ values for DPPH and hydroxyl radical scavenging activities of the final 9th-cycling product, decreased 48.4% and 76%, respectively, compared with those of Rehmannia glutinosa. Our result was suggested that antioxidant activities of Rehmannia radix Preparata improve according to the increasing number of steaming-drying cycles.

Keywords

References

  1. Ahn DK, Kim CM, Shin MK and Lee KS. (1998). Traditional chinese medicine dictionary. Chungdambooks, Seoul. Korea. 2:168-176.
  2. Ahn SW, Kim YG, Kim MH, Lee HY and Seong NS. (1999). Comparison of biological activities on Rhemannia Radix and R. Radix Preparata produced in Korea. Korean Journal of Medicinal Crop Science. 7:257-262.
  3. Benzie IF and Strain JJ. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "Antioxidant power": The FRAP assay. Analytical Biochemistry. 230:70-79.
  4. Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 26:1199-1200.
  5. Cerutti PA. (1985). Peroxidant states and tumor promotion. Science. 227:375-381. https://doi.org/10.1126/science.2981433
  6. Chun JC, Kim JC, Hwang IT and Kim SE. (2002). Acteoside from Rehmanniae glutinosa nillities paraquat activity in Cucumis sativua. Pesticide Biochemistry and Physiology. 72:153-159. https://doi.org/10.1016/S0048-3575(02)00008-1
  7. Chun JC, Ma SY, Kim SE and Lee HJ. (1997). Physiological responses of Rehmanniae glutinosa to paraquat and its tolerance mechanisms. Pesticide Biochemistry and Physiology. 59:51-63. https://doi.org/10.1006/pest.1997.2307
  8. Chung SK. (1997). Hydroxy radical scavenging effects of species and scavengers from brown mustard. Bioscience Biotechnology and Biochemistry. 61:118-123. https://doi.org/10.1271/bbb.61.118
  9. Cohen G. (1988). Oxygen radicals and Parkinson's disease. In Halliwell B, (ed.). Oxygen Radicals and Tissue Injury. Ferderation of American Societies for Experimental Biology. Bethesda, MD. USA. p. 130-135.
  10. Doh ES, Chang JP, Lee KH and Seong NS. (2010). Ginsenoside change and antioxidation activity of fermented ginseng. Korean Journal of Medicinal Crop Science. 18:255-265.
  11. Halliwell B and Gutteridge JC. (1984). Oxygen toxicity, radicals, transition metal and disease. Biochemical Journal. 319:1-14.
  12. Hwang SY, Hwang BY, Choi WH, Jung HJ, Huh JD, Lee KS and Ro JS. (2001). Quantitative determination of 5- hydroxymethyl-2-furaldehyde in the Rehmanniae radix Preparata samples at various processing stages. Korean Journal of Pharmaconology. 32:116-120.
  13. Kang MH, Choi CS, Kim ZS, Chung HK, Min KS, Park CG and Park HW. (2002). Antioxidative activities of ethanol extract prepared from leaves, seed, branch, and aerial part of Crotalaria sessiflora L. Korean Journal of Food Science Technology. 34:1098-1102.
  14. Kim DW, Lee YJ, Min WJ, Kim YJ, Rho YD and Yang DC. (2009). Conversion of acidic polysaccharide and phenolics compound of changed ginseng by 9 repetitive steaming and drying process, and its effects of antioxidation. Korean Journal of Oriental Physiology & Pathology. 23:121-126.
  15. Kim HJ, Lee JY, You BR, Kim HR, Choi JE, Nam KY, Moon BD and Kim MR. (2011). Antioxidant activities of ethanol extracts from black ginseng prepared by steaming-drying cycles. Journal of Korean Society Food Science Nutrition. 40:156-162. https://doi.org/10.3746/jkfn.2011.40.2.156
  16. Kim TH, Kim JM, Beak JM, Kim TW, kim DJ, Park JH and Choe M. (2011). Antioxidant and whitening effects of Agrimonia pilosa Ledeb water extract. Korean Journal of Medicinal Crop Science. 19:177-184. https://doi.org/10.7783/KJMCS.2011.19.3.177
  17. Kim YC, Cho CW, Rhee YK, Yoo KM and Rho JH. (2007). Antioxidant activity of ginseng extracts prepared by enzyme and heat treatment. Journal of Korean Society Food Science Nutrition. 39:1482-1485.
  18. Lee CK and Seo JM. (2004). Changes of the constituents in the Rehmanniae radix Preaparata during processing. Journal of Korean Society Food Science Nutrition. 33:1748-1752. https://doi.org/10.3746/jkfn.2004.33.10.1748
  19. Lee JH, Koh JA, Hwang EY and Hong SP. (2002). Quantitative determination of 5-hydroxymethyl-2-furaldehyde from Rehmanniae radix Preparata according to various processing. Korean Journal of Herbology. 17:145-149.
  20. Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY and Chung IM. (2004). Comparison of SOD Activity and phenolics compound contents in various Korean medicinal plants. Korean Journal of Medicinal Crop Science. 12:191-202.
  21. Ma JY, Ha CS, Sung HJ and Zee OP. (2002). Hemopoietic effects of rhizoma Rehmanniae Preparata on cyclophosphamideinduced prenicious anemia rats. Korean Journal of Pharmacology. 31:325-334.
  22. Morota T, Sasaki H, Nishimura H, Sungama K, Chin M and Mitsahashi H. (1989). Two iridoid glycosides from Rehmannia glutinosa. Phytochemistry. 28:2149-2153. https://doi.org/10.1016/S0031-9422(00)97934-2
  23. Park BY, Chang SM and Choi J. (1989). Relationships between the inorganic constituents contents and the catalpol and sugar contents in the rhizoma of Rehmannia glutinosa. Journal of Korean Agricultural Chemistry Society. 32:249-254.
  24. Park NK, Kim SL, Hur HS and Park CH. (2002). Development of R. radix Preparata with new variety "Jiwhang1". Korean Journal of International Agriculture. 14:3-39.
  25. Pellegrini N, Re R, Yang M and Rice-Evans V. (1998). Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Methods of Enzymology. 299:379-389.
  26. Singleton VL and Rossi JA. (1965). Colorimetry of total phenolics with phosphornolybdic-phosphotungstic acid reagents. American Journal of Enology Viticulture. 16:144-158.
  27. Woo KS, Song DS, Lee JS, Lee HB and Jeong HS. (2007). Quality characteristics of Rehmannia radix Preparata with presoaking solvents. Korean Journal of Food Science Technology. 39:289-294.
  28. You BR, Kim HR, Kim HJ, Lee JY, Lee SY, Song MR, Park JY and Kim MR. (2011). Catalpol content an antioxidant activities in various cultivars of Rehmanniae glutinosa. Journal of Korean Society Food Science Nutrition. 40:481-485. https://doi.org/10.3746/jkfn.2011.40.4.481
  29. Yu MH, Im HG, Lee HJ, Ji YJ and Lee IS. (2006). Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus juiuba var. inermis rehder. Korean Journal of Food Science Technology. 38:128-134.