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Development of Near-Isogenic Lines (NILs) Conferring Xa4, xa5 and Xa21 Genes Resistant to Bacterial Blight (Xanthomonas oryzae pv. oryzae) in japonica rice Genetic Background

  • Kim, Ki-Young;Shin, Mun-Sik;Kim, Woo-Jae;Park, Hyun-Su;Ko, Jong-Cheol;Nam, Jeong-Kwon;Shin, Woon-Chul;Mo, Young-Jun;Jeung, Ji-Ung;Kim, Bo-Kyeong;Ko, Jae-Kwon
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.383-390
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    • 2011
  • Near-isogenic lines (NILs) carrying bacterial blight resistance genes (Xa4, xa5 and Xa21) were developed in japonica rice using Suweon345 as genetic background. NILs were selected by gene specific DNA markers and inoculation of K1 or K3a race. NILs conferring Xa4 were resistant to K1, K2, K3, and moderately resistant to K3a. NILs conferring xa5 were resistant to K1, K2, K3, and K3a. NILs having Xa21 were susceptible to K1, while resistant to K2, K3 and K3a. Target genes of NILs with the genetic background of Suweon345 were also confirmed by using eleven Philippines races and International Rice Bacterial Blight (IRBB) NILs carrying Xa4, xa5 and Xa21. All NILs had no significant difference from their recurrent parents in the major agronomic traits except for panicle length and brown rice 1,000 grain weight. Heading date of NILs ranged from Aug. 10 to Aug. 11, which was similar to that of recurrent parent, Suweon345. Culm length, number of grains per panicle and ratio of ripened grain of NILs were similar to those of Suweon345. Milled rice of NILs was ranged from 4.82 to 4.93MT/ha. These NILs will be useful for improving resistance to K3a race of bacterial blight pathogens in Korean japonica cultivars.

A study to effectiveness the usefulness of skin patches containing chlorogenic acid and garcinia (클로로겐산이 함유된 피부 패치의 유용성 평가를 위한 연구)

  • Shin, Joo-Yeon;Ko, Kyel;Kwon, Byong-An
    • Journal of Digital Convergence
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    • v.19 no.3
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    • pp.371-378
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    • 2021
  • The purpose of study was to evaluate the usefulness a skin patch containing chlorogenic acid to the skin to identify changes in body fat and weight. 16 volunteers, consisting of 8 experimental groups and 8 control groups, participated in the study, and the study period was conducted for 4 weeks from Aug 03, 2020 to Aug 31, 2020. As a result of the inter-group effect test, there was no significant difference in body fat (F=4.38, p<.055), but it can be said that the experimental group had a positive effect because the difference in posterior score was larger than that of the control group. In the change of body weight, it was found that there was a significant difference in weight loss in the experimental group (F=9.43, p<.008). It will be meaningful as a preliminary study applying patches to studies related to obesity, and we hope that the results of the study will be used as basic data for research on patch materials in the future.

Breeding of 'Jinmani' Cultivar of Gomchwi with Disease Resistance, High Quality and Yield

  • Jong Taek Suh;Ki Deog Kim;Jong Nam Lee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.18-18
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    • 2021
  • Gomchwi using stuffed leaves is many cultivated for 'Gondalbi(Ligularia stenocephala)' among Gomchwi species. 'Gondalbi' species like to be cultivation on farm because of low the incense and the bitter taste, and high yield. But 'Gondalbi' caused to curtail yield that susceptibility of powdery mildew disease and shriveling and death of plant on summer season. To solve this problems, we crossed a Gomchwi and 'Handeari-gomchwi' to have resistance of powdery mildew disease and high yield. A new Gomchwi cultivar 'Jinmani' was bred by crossing between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The selection and investigation of growth and yield characteristics were conducted from 2007 to 2020 in field and greenhouse of Highland Agriculture Research Institute, NICS, Rural Development Administration. The color of petiole ear was purple. trichome of petiole and leaf back non-existed, and luster of leaf back existed. Density of leaf vein was 4 degree among 1-5 degree in a newly developed cultivar 'Jinmani'. Plant height, leaf length, leaf width and petiole length were 55.7, 21.8, 22.2, and 33.9 cm, respectively in the 2nd year of growth characteristics. Plant size was similar with that of 'Gommany'. Bolting and flowering time were Aug. 5 and Sept. 5, respectively, and Bolting and flowering time of 'Gommany' showed similar to Aug. 8 and Sept. 1, respectively. 'Jinmani' showed higher number of leaves (202 ea.) per plant compared to 'Gommany' (159 ea.). Furthermore, yield was 67.9% higher in 'Jinmani' (2,569 g/plant) than in 'Gommany' (1,530 g/plant). 'Jinmani' showed lower leaf thickness (0.66mm) than 'Gommany' (0.69 mm), and consequently showed more hardness in leaf characteristics (25.1 kg/2) compared to 'Gommany' (24.3kg/cm2). 'Jinmani' showed similar strong resistance compared to 'Gommany' in the susceptibility of powdery mildew disease.

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Changes of Green Manure and Nitrogen Yield of Hairy Vetch According to Seeding Date in Autumn (헤어리베치의 추파시기에 따른 녹비의 수량 및 질소량 변화)

  • 서종호;이호진;김시주
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.6
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    • pp.400-404
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    • 2000
  • Hairy vetch (vicia villosa Roth), leguminous green manure crop, can increase soil fertility and reduce chemical nitrogen application for cash crop such as spring corn. More yield of hairy vetch is needed at planting cash crop to obtain higher effect of green manure. Hairy vetch waa seeded on Sep.10, Oct.1, and Oct. 20 and at 10, 20, 30 and 40 kg/ha of seeding rate respectively, in 1996 and 1997, Dry matter and nitrogen yield of hairy vetch were measured on May 1 in 1997 and 1998. Proper seeding rate of hairy vetch was 30 kg/ha irrespective of years and seeding dates. Above-ground dry matters of hairy vetch on May 1 in 1997 and 1998 decreased according to delayed seeding, and those were 5.5-7, 4-4.5, 1.3-2.2 ton/ha on Sep. 10, Oct. 1 and Oct 20 of seeding date, respectively in seeding rate 30-40 kg/ha. Also nitrogen yield of hairy vetch on May 1 decreased according to delayed seeding, and those were 220-280, 160-180, 60-100kg/ha on Sep. 10, Oct. 1 and Oct 20 of seeding date, respectively in seeding rate 30-40kg/ha. Therefore, we suggest that hairy vetch has to be seeded earlier in autumn to obtain high green manure yield in spring. To determine the detailed optimum seeding time in autumn, hairy vetch was seeded on Aug. 20, Aug. 31, Sep. 10, Sep. 20, and Sep. 30 in 1999 and was harvested on April 22, April 27, and May 2 in 2000, respectively. Dry matter and nitrogen yield of hairy vetch by seeding in late August were higher than those by seeding in September indicating that dry matter of hairy vetch were 7-8, 6-7, 4-5, 2-3 ton/ha and nitrogen yield were 240-290, 200-260, 150-220, 70-120 kg/ha, respectively when seeded on Aug. 20, Aug. 31, Sep. 10, Sep. 20 and Sep. 30 and harvested on April 22-May 2. Increase of dry matter and N yield of hairy vetch by 10days delayed harvest was higher in late August seeding than in September seeding. So hairy vetch should be seeded in late August if possible to obtain much more green manure yield and be seeded until September because green manure yield decrease rapidly when seeded after October.

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Vine Growth and Fruit Characteristics of 'Jinok' and 'Campbell Early' Grape as Influenced by Cropping System (재배작형에 따른 포도 '진옥'과 '캠벨얼리'의 수체생육 및 과실 특성 분석)

  • Cheon, Mi Geon;Kim, Yeong Bong;Lee, Sun Yeong;Hong, Gwang Pyo;Jung, Sung Min;Kim, Jin Gook
    • Journal of Bio-Environment Control
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    • v.26 no.3
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    • pp.175-180
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    • 2017
  • This study was conducted for the domestic new cultivar to expand cultivation area and sustainable production. The new domestic cultivar, 'Jinok' and control cultivar, 'Campbell Early', had been investigated on vine growth and fruit quality by different cultivating conditions as open field, rain shelter and unheated plastic house. The growth period of 'Jinok' was faster than 'Campbell Early'. The harvesting date of 'Jinok' was on 13th of Aug. faster than 'Campbell Early' which was on 17th of Aug., and it was on 25th and 29th of Aug. for 'Jinok' and 'Campbell Early' on field cultivation, respectively. The growing length and width of new shoots were similar among the cropping systems. In the result of fruit characteristic evaluation, the soluble solids content of the 'Jinok' and 'Campbell Early' cultivating on the unheated plastic house were $15.4^{\circ}Brix$ and $15.9^{\circ}Brix$, respectively. In the open field condition, those were $15.9^{\circ}Brix$ and $15.8^{\circ}Brix$, respectively. The titratable acidity and chromaticity were similar among the treatments. In the appearance of past and disease, Phomopsis blight was occurred on mid of June and in the end of cultivation period on about early Aug., the damage by Phomopsis blight was the lowest about 14% in the unheated plastic house cultivation on the 'Jinok' cultivar and it was 39% in the open field condition. However, in the open filed cultivation for 'Campbell Early', Phomopsis blight was highly occurred about 49%. During this period, or the end of cultivation, it is necessary for the intensive control.

Effect of Different Cultivation Region, Transplanting and Harvesting Date on Yield and Quality of "Shinongheugchal", "Shinmyeongheugchal" (재배지역, 이앙 및 수확시기가 신농흑찰, 신명흑찰 수량 및 품질에 미치는 영향)

  • Song, Young-Eun;Lee, Deok-Ryeol;Cho, Seung-Hyun;Lee, Ki-Kwon;Lee, Jae-Heung;Song, Young-Ju;Jeung, Jong-Sung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.248-253
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    • 2012
  • Demand is increasing for new functional and health food such as natually colored or flavored rices. Shinongheugchal, Shinmyeongheugchal, colored glutinous rice, were breed as new variety in Jeollabuk-do ARES. Shinongheugchal and Shinmyeongheugchal are medium to late maturing cultivars and higher than Heugnambyeo in term of C3G content. It was conducted to improve the quality of Shinongheugchal, Shinmyeongheugchal in different cultivation regions, transplanting time. The cultivation regions was Jinan (mid-mountainous area) and Iksan (plain area). Transplanting time was May. 20, May. 25 May 30 and Jun. 5 in Jinan, that of Iksan was Jun. 5, Jun. 10, Jun. 15 and Jun. 20. The average heading date of Shinongheugchal and Shinmyeongheugchal was Aug. 13, Aug. 19 and Aug. 15, Aug. 22 in Jinan and Iksan. The average yield of Shinongheugchal(brown rice) in Jinan was 514 $kg\;10a^{-1}$ and that of in Iksan was 529 $kg\;10a^{-1}$. The average yield of Shinmyeongheugchal(brown rice) in Jinan was 508 $kg\;10a^{-1}$ and that of in Iksan was 511 $kg\;10a^{-1}$. The average C3G content of Shinongheugchal in Jinan was 273.9 mg/100g, that of in Iksan was 228.2 mg/100g. The average C3G content of Shinmyeongheugchal in Jinan was 126.3 mg/100g, that of in Iksan was 101 mg/100g. The optimal transplanting date was May 30 in Jinan and June 10 in Iksan considering the yield and C3G content in Shinongheugchal. The optimal harvesting date of Shinongheugchal in Iksan was 50 days after heading.

Influences of Sowing Time and Nursery Period on Growth and Yield of Perilla frutescens BRITTON var. acuta KUDO (파종기(播種期) 및 육묘기간(育苗期間)이 자소(紫蘇)의 생육(生育) 및 수량(收量)에 미치는 영향(影響))

  • Park, Hi-Jin;Chung, Dong-Hee;Kim, Sang-Gon;Kwon, Byung-Sun
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.1
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    • pp.1-4
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    • 1995
  • To determine the optimum sowing time and nursery period in Perilla frutescens in the southern areas of Korea, perilla frutescens cv. red Perilla local cultivar was grown under three different sowing dates and nursery periods. The blooming period of the area which was sown on the seedbed in Apr.10 and carried out the growing seedling in 30days is Aug. 12 and it is two days earlier than that of the area, Aug.14, sown on the seedbed in Apr. 20 and carried out the growing seedling in 30days of the same month and it is also six days earlier than that of the area, Aug.18, sown on the seedbed in April 30 and conducted the growing seedling in the same date. The stem lengths are 135cm,131cm and 125cm respectively and the number of branches are 26.4, 25.3 and 23.6 respectively. The fresh weight of stem and leaf at the area sown on the seedbed in Apr.10 and conducted the growing seedling in 30days with the width of leaf over 5cm in the middle of Aug. and at the beginning of Sep. is 2,476kg/10a and it shows more increase of 172kg than that of fresh weight of stem and leaf with 2,304kg/10a which was sown on the seedbed in Apr.20 and earned out the growing seedling in 30days, and it also shows more increase of 411kg than that of fresh weight of stem and leaf with 2,065kg/10a at the area sown on the seedbed in April 30 and con­ducted the growing seedling in the same date, The fresh weight of seeds are 609.5kg/10a,509.3kg/10a and 463.2kg/10a respectively and $100.2{\sim}146.3kg$ is more increased. Therefore, the seedling period of the proper seedbed for high yield bumper crop of perilla frutescens for exporting to Japan is April 10 and the number of days for seedling is 30 days.

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Changes in Physicochemical Properties and Antioxidant Activities according to Different Harvest Times in Black Rice (Oryza sativa L.) (흑미 수확시기에 따른 이화학적 특성 및 항산화 활성 변화)

  • Park, Ji-Young;Han, Sang-Ik;Hur, Yeon-jae;Lee, Yu-Young;Lee, Byung-Won;Sim, Eun-Yeong;Ham, Hyeonmi;Kim, Byeong-Ju;Lee, Chun-Woo;Lee, Sung-Joon;Oh, Sung-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.11
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    • pp.1653-1659
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    • 2015
  • This study compared physicochemical properties and antioxidant activities according to different harvest times in five varieties of black rice (BR). Five cultivars of BR, Nunkeunheukchal (NKHC), Sinnongheugchal (SNHC), Sintoheugmi (STHM), Josaengheugchal (JSHC), and Heugjinju (HJJ), were selected for the study. Cultivars were transplanted on June 20th and cultivated by the Department of Southern Area Crop Science, NICS in Miryang, Korea. Heading dates of NKHC, SNHC, STHM, JSHC, and HJJ were Aug. 17th, Aug. 30th, Sep. 1st, Aug. 20th, and Aug. 12th, respectively. The five different harvest times were as follows; 35, 40, 45, 50, and 55 days after heading date. The highest level of anthocyanin was 35 days after heading date in all varieties. Levels of anthocyanin in NKHC, SNHC, STHM, JSHC, and HJJ were 164, 203, 251, 67, and 210 mg/100 g, respectively. Content of anthocyanin significantly decreased 35 days after heading in four varieties, excluding JSHC. Highest DPPH $IC_{50}$ values were 57, 29, 10, and $27{\mu}g/mL$ in NKHC, SN HC, STHM, and JSHC extracts, respectively. Highest ABTS $IC_{50}$ values were 24, 13, 9, and $11{\mu}g/mL$ in NKHC, SNHC, STHM, and JSHC extracts, respectively. The antioxidant activities were highest 35 days after the heading date and significantly decreased in four varieties, excluding HJJ. The antioxidant activity of HJJ showed no significant difference, according to harvest time. This study suggests that the content of anthocyanin and antioxidant activity could be considered as factors affecting optimal harvest period to produce highly qualified black rice.

An Analysis of Response Spectrums of Earthquakes of Korean Peninsula in the First Half of 2000 (2000년도 상반기 한반도 발생지진들의 응답 스펙트럼 분석)

  • 이전희
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2000.10a
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    • pp.66-72
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    • 2000
  • We have scanned the several seismic traces of earthquakes and blasts observed from the digital new type seismograph instruments of KMA from Jan. 2000 to Aug. 2000. From these data, good quality data which have high signal/noise ratio were selected and they were transformed into ascii data from binary data(mini-seed format). The hypo71 program and P-S was applied in order to determine the location of epicenter, origin time and the magnitude. From these data, the 18 earthquakes and 3 blasts, 207 seismic records consist of 359 directional components were calculated. Using theses ground acceleration data, acceleration, velocity, and displacement response spectrums of the structures were calculated and they could be represented in a picture by the form of tripartite response spectrum. In the result, response spectrums of the 359 directional components of the above seismic data records were obtained respectively.

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The Structural Model about Impact of Nurse's Career Management, Career Plateau, Career Satisfaction on Career Commitment (간호사의 경력관리, 경력정체, 경력만족이 경력몰입에 미치는 영향에 관한 구조모형)

  • Moon, Inn-Oh
    • Journal of Korean Academy of Nursing Administration
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    • v.16 no.2
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    • pp.180-189
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    • 2010
  • Purpose: This study was designed to construct a model which explains the career management, career plateau and career satisfaction of the factors influencing the career commitment of nurses. Method: The data collection was conducted from Aug. 24, 2009 to Sep. 22, 2009 through self-reported questionaries. Participants were 441 nurses who had worked in 2 national university hospitals, 1 university hospitals, and 1 hospitals founded by business enterprises. The data was analyzed by SPSS 12.0 and AMOS 15.0 for structural model. Results: Career commitment was directly impacted by career management, career plateau, career satisfaction. The predictable variables of the hypothetical model explained 28.4% of career commitment. Career commitment was significantly influenced by career management, career plateau, career satisfaction. Conclusion: The results of study suggest that some strategies are needed which focus on career management, career plateau and career satisfaction in order to promote the nurse's career commitment.