• Journal of Korea Multimedia Society
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• v.15 no.1
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• pp.71-80
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• 2012

LBS (Location Based Service) services are very useful to our daily life and it should be available inside a building: a huge building, a shopping mall, a large scale underground shopping center, and so on where GPS (Global Positioning System) signal is not available. An LBS which is provided inside a building is called an ILBS (Indoor Location Based Service). One of the most important techniques for ILBS development is indoor positioning. Among the indoor positioning techniques, APs (access points) of WLAN based ones are most economical because WLAN is available almost everywhere. Meanwhile, the web service has been proved to be an excellent practice of software reuse. Therefore, if indoor positioning is provided in the form of web service to programmers then development of ILBS would be greatly accelerated. This paper introduces the AP based trilateration and K-NN indoor positioning and RFID based indoor positioning web services we have developed.

• Journal of Satellite, Information and Communications
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• v.12 no.3
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• pp.98-102
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• 2017

In this paper, we study RF-powered backscatter cognitive radio networks to improve the performance for the secondary user which is backscatter radio based wireless sensors. In our proposed model, we consider an avoiding the doubly round-trip attenuation to add a carrier emitter and utilization of spectrum sensing information. When the primary channel is busy, the secondary user is able to harvest RF energy from the channel through a hybrid-access point (H-AP) and a carrier emitter. When the channel becomes idle, the secondary user will be use the harvested energy to operate wireless sensors, to use the sensing and to backscatter through the carrier emitter. We model mathematically the deterministic and multisource elements of a number of tagged channels. In the proposed communication environment, we show the BER performance of the backscatter communication using WiFi signal.

• Journal of Advanced Navigation Technology
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• v.19 no.6
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• pp.558-563
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• 2015

There have recently been various services that use indoor location estimation technologies. Representative methods of location estimation include fingerprinting and triangulation, but they lack accuracy. Various kinds of research which apply existing location estimation methods like AOA, TOA, and TDOA are being done to solve this problem. In this paper, we study the location estimation algorithm based on AOA using a RSSI difference in indoor environments. We assume that there is a single AP with four antennas, and estimate the angle of arrival based on the RSSI value to apply the AOA algorithm. To compensate for RSSI, we use a recursive averaging filter, and use the corrected RSSI and the Pythagorean theorem to estimate the angle of arrival. The results of the experiment, show an error of 18% because of the radiation pattern of the four non-directional antennas arranged at narrow intervals.

• BMB Reports
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• v.30 no.1
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• pp.46-54
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• 1997

Alkaliphilic Bacillus sp. S-1 secretes a large amount (approximately 80% of total pullulanase activity) of an extracellular pullulanase (PUL-E). The pullulanase exists in two forms: a precursor form (PUL-I: $M_r$ 180,000), and a processed form (PUL-E: $M_r$ 140,000). Two forms were purified to homogeneity and their properties were compared. PUL-I was different in molecular weight, isoelectric point, $NH_2$-terminal amino acid sequence, and stabilities over pH and temperature ranges. The catalytic activities of PUL-I were also distinguishable in the $K_m$ and $V_{max}$ values for various substrates, and in the specific activity for pullulan hydrolysis. PUL-E showed 10-fold higher specific activities than PUL-I. However. PUL-I is immunologically identical to PUL-E, suggesting that PUL-I is initially synthesized and proteolytically processed to the mature form of PUL-E. Processing was inhibited by PMSF, but not by pepstatin, suggesting that some intracellular serine proteases could be responsible for processing of the PUL-I. PUL-I has a different conformational structure for antibody recognition from that of PUL-E. It is also postulated that the translocation of alkaline pullulanase(AP) in the bacterium possibly requires processing of the $NH_2$-terminal region of the AP protein. Processing of the precursor involves a conformational shift. resulting in a mature form. Therefore. precursor processing not only cleaves the signal peptide, but also induces conformational shift. allowing development of active form of the enzyme.

• International Journal of Oral Biology
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• v.35 no.2
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• pp.43-49
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• 2010

Enterococcus faecalis, a gram-positive bacterium, has been implicated in endodontic infections, particularly in chronic apical periodontitis. Proinflammatory cytokines, including tumor necrosis factor-$\alpha$ (TNF-$\alpha$), are involved in the pathogenesis of these apical lesions. E. faecalis has been reported to stimulate macrophages to produce TNF-$\alpha$. The present study investigated the mechanisms involved in TNF-$\alpha$ production by a murine macrophage cell line, RAW 264.7 in response to exposure to E. faecalis. Both live and heat-killed E. faecalis induced high levels of gene expression and protein release of TNF-$\alpha$. Treatment of RAW 264.7 cells with cytochalasin D, an inhibitor of endocytosis, prevented the mRNA up-regulation of TNF-$\alpha$ by E. faecalis. In addition, antioxidant treatment reduced TNF-$\alpha$ production to baseline levels. Inhibition of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein (MAP) kinase also significantly attenuated E. faecalis-induced TNF-$\alpha$ expression by RAW 264.7 cells. Furthermore, activation of NF-${\kappa}B$ and AP-1 in RAW 264.7 cells was also stimulated by E. faecalis. These results suggest that the phagocytic uptake of bacteria is necessary for the induction of TNF-$\alpha$ in E. faecalis-stimulated macrophages, and that the underlying intracellular signaling pathways involve reactive oxygen species, ERK, p38 MAP kinase, NF-${\kappa}B$, and AP-1.

• Journal of the Korean Electrochemical Society
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• v.10 no.2
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• pp.150-154
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• 2007

Redox complexes to transport electrodes from biomaterial to electrodes are very important part in commercial biosensor industry. A novel osmium redox complex was synthesized by the coordinating pyridine group with osmium metal. A novel osmium complex is described as $[Os(dme-bpy)_2(ap-im)Cl]^{+/2+}$. We have been studied the electrochemical characteristics of this osmium complex with electrochemical techniques such as cyclic voltammetry and chronoamperommetry. In order to immobilize osmium redox complexes on the electrode, we deposited gold nano-particles on screen printed carbon electrode(SPE). The electrical signal converts the osmium redox films into an electrocatalyst for glucose oxidation. The catalytic currents were monitored that the catalytic currents were linearly increased from 1 mM to 5 mM concentrations of glucose.

• Molecules and Cells
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• v.40 no.10
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• pp.706-713
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• 2017

Osteoclasts are bone-resorbing cells that are derived from hematopoietic precursor cells and require macrophage-colony stimulating factor and receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL) for their survival, proliferation, differentiation, and activation. The binding of RANKL to its receptor RANK triggers osteoclast precursors to differentiate into osteoclasts. This process depends on RANKL-RANK signaling, which is temporally regulated by various adaptor proteins and kinases. Here we summarize the current understanding of the mechanisms that regulate RANK signaling during osteoclastogenesis. In the early stage, RANK signaling is mediated by recruiting adaptor molecules such as tumor necrosis factor receptorassociated factor 6 (TRAF6), which leads to the activation of mitogen-activated protein kinases (MAPKs), and the transcription factors nuclear factor-${\kappa}B$ (NF-${\kappa}B$) and activator protein-1 (AP-1). Activated NF-${\kappa}B$ induces the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), which is the key osteoclastogenesis regulator. In the intermediate stage of signaling, the co-stimulatory signal induces $Ca^{2+}$ oscillation via activated phospholipase $C{\gamma}2$ ($PLC{\gamma}2$) together with c-Fos/AP-1, wherein $Ca^{2+}$ signaling facilitates the robust production of NFATc1. In the late stage of osteoclastogenesis, NFATc1 translocates into the nucleus where it induces numerous osteoclast-specific target genes that are responsible for cell fusion and function.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.2
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• pp.1250-1264
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• 2017

This paper presents the architecture design, implement, experimental validation of a bistatic backscatter wireless communication system in Wi-Fi network. The operating principle is to communicate a tag's data by detecting the power level of the power modulated Wi-Fi packets to be reflected or absorbed by backscatter tag, in interconnecting with Wi-Fi device and Wi-Fi AP. This system is able to provide the identification and sensor data of tag on the internet connectivity without requiring extra device for reading data, because this uses an existing Wi-Fi AP infrastructure. The backscatter tag consists of Wi-Fi energy harvesting part and a backscatter transmitter/a power-detecting receiver part. This tag can operate by harvesting and generating energy from Wi-Fi signal power. Wi-Fi device decodes information of the tag data by recognizing the power level of the backscattered Wi-Fi packets. Wi-Fi device receives the backscattered Wi-Fi packets and generates the tag's data pattern in the time-series of channel state information (CSI) values. We believe that this system can be achieved wireless connectivity for ultra- low-power IoT and wearable device.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.7 no.1
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• pp.50-55
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• 1996

Acoustic analysis study was performed on 20 normal subjects by speaking nonsense syllables composed of Korean bilabial stops$(/P, P^{\star}, P^{h}/)$ and their preceding and/or following vowel /a/ (that is, $[pa, p^{\star}a, p^{h}a, apa, ap^{\star}a, ap^{h}a]$) with an ultraminiature pressure, sensor. in their mouths. Speech materials were phonated twice, once with a moderate voice, another time with a loud voice. The acoustic signal and intraoral pressure were recorded simultaneously on computer. By these procedures, we were to measure the intraoral pressure, closure duration and VOT of Korean bilabial stops, and to compare the values one another according to the intensity of phonation and the position of the target consonants. Intraoral pressure was measured by the peak intraoral pressure value of Its wave closure duration by the time interval between the onset of intraoral pressure build-up and the burst meaning the release of closure ; Voice onset time(VOT) on by the time interval between the burst and the onset or glottal vibration. Heavily aspirated bilabial stop consonant /$p^h$/ showed the highest intraoral pressure value, unaspirated /$p^{\star}$/, the second, slightly aspirated /P/, the lowest. The syllable initial bilabial stops showed higher intraoral pressure than word initial stops, and the value of loudly phonated consonants were higher than moderate consonants. The longest closure duration period was that of /$p^{\star}$/ and the shortest, /P/, and the duration was longer in word initial position and in the moderate voice. In VOT, the order of the longest to shortest was $/{p^h}/, /p/, /{p^\star}/$, and the value was shorer when the consonant was in intervocalic position and when it was phonated with a loud voice.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.44 no.5
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• pp.16-24
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• 2007

Wireless mesh network extends the limited coverage of conventional wireless service from one-hop access point to wider area. It is recognized as an important issue to provide seamless handoff in the wide coverage of wireless mesh network. A long handoff delay may cause high packet loss and disconnection of service, degrading the network performance. The handoff delay is mainly introduced from the channel scanning process obtaining information to make handoff decision and the unnecessary handoffs due to inaccurate handoff decision. In this paper, we propose the handoff algorithm based on one-way trip time (OTT) to expedite the handoff procedure. As compared to the handoff algorithm based on the receiving signal power, the proposed algorithm takes advantage of OTT to obtain the necessary handoff information in a relatively shorter time and reduce the unnecessary handoffs. The performance of proposed algorithm is evaluated through the simulations. It is verified that the proposed handoff algorithm can effectively enhance the handoff accuracy, and therefore reduce the handoff delay and unnecessary handoffs.