• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.35 no.3
• /
• pp.179-183
• /
• 1990

In order to find out the effects of nitrogen fertilizer, planting density and seeding date of Okra (Abelmoschus esculentus (L) MOENCH), three nitrogen fertilizer level (4, 8 and 10kg N/10a), three planting densities (45${\times}$30cm, 60${\times}$30cm and 90${\times}$30cm) and five seeding date (April 15, May 1, May 15, June 1 and June 15) were treated. Treatment of 8kg N/10a among nitrogen fertilizer level and 45${\times}$30cm among planting densities showed higher green and yield than the other treatments. The plot of 45${\times}$30cm with 8kg N/10a showed the highest yield. Among planting date, May 1 planting date obtained the highest yield.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.4 no.2
• /
• pp.98-107
• /
• 1983

This experiment was carried out to determine the optimum fertilizer level of N-P-K for maximum yield of grasses at hill-land pasture. The pasture species tested were orchardgrass, tall fescue, alfalfa and ladino clover. The results are summarized as follows: 1. The difference in yield of treatment 1, 2, and 4, 5 was significant at the 5% level. In other word, alfalfa yields from 6-30-30 kg/10a and 6-40-30 kg/10a (N-P-K) application were significantly increased over the yields from 4-20-20 kg/10a and 4-30-20 kg/10a (N-P-K). In observing the yield from the treatment 5 where 10 kilograms of phosphate was additionally applied over the treatment 4, it was increased in yield but failed to show statistical significance. With these results, the most optimum rates of N-P-K for alfalfa production are 6-30-30 kg/10a. 2. Ladino clover showed the highest yield at treatment 6 with statistic significance at the 1 percent level over the other treatments. Therefore, NPK rates of 6-30-40 kg/10a would be the most optimal rates for maximum production of ladino clover. 3. Orchard grass yield showed significance at the 1 percent level among other treatments except treatment 3 and 4. The highest yield was also shown at treatment 6 where NPK rates applied 40-30-40 kg/10a. 4. Tall fescue yield showed high significance at the 1 percent except treatments between 2 and 3, 5 and 6. The highest yield was observed at treatment 6 where NPK was applied 40-30-40 kilograms per 10a. 5. Nitrogen content of dry matter was 0.91-1.05% in 20 kg of N and 1.05-1.26% in 30-40 kg of N per 10a. The absorption rate of nitrogen applied was 23-31% regardless of the nitrogen level. Potash content of dry matter was increased in accordance with increasing K rates. 6. The gross income was the highest on the N-P-K application of 4-20-30 kg/10a in alfalfa and ladion clover, 40-30-40 kg/10a in orchardgrass and 30-20-20 kg/10a in tall fescus.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.10
• /
• pp.4939-4941
• /
• 2012

Background: The serum level of soluble CD30 (sCD30) is known to be increased with several lymphomas and to correlate with prognosis. Primary effusion lymphoma (PEL) is a highly aggressive malignant lymphoma with poor prognosis, but the existence and significance of sCD30 in PEL have not yet been investigated in detail. Objectives: Since the membrane type of CD30 is frequently expressed on the surface of PEL cells, we compared the expression of the membrane type of CD30 and the production of sCD30 among PEL cell lines as well as other lymphomas. Methods: The expression of surface CD30 in various lymphoma cell lines was analyzed with flow cytometry ans sCD30 was quantified by ELISA. Results: Both surface and sCD30 were detected on PEL cell lines as well as on Hodgkin's lymphoma and adult T-cell leukemia/lymphoma cell lines. Surface CD30 and sCD30 levels of each cell lines correlated with each other. Conclusion: The serum level of sCD30 appear to be a useful biological tumor marker for the diagnosis and management of CD30-positive PEL.

• Journal of the Korean Chemical Society
• /
• v.34 no.6
• /
• pp.509-516
• /
• 1990

1H-nmr chemical shifts and lineshapes of amino-protons of acetamide (AA) in n-alcohols were determined. The chemical shifts are discussed by the Reichardt's solvent polarity parameter, E$_{T}$(30). The following relationship between $\delta$obs and E$_{T}$(30) was obtained. ${\delta}_{obs}$ = ${\delta}_{o}$ ＋ aE$_{T}$ (30) ＋ b[E$_{T}$(30)]$^2$ where ${\delta}_{o}$ is the chemical shift of the solute in gaseous state or at $E_{T}$(30) = 0, a is a characteristic constant for the protons of AA in n-alcohol solutions and b is a constant for the solute (AA)-solvent (n-alcohols) interaction. The barrier of the hindered rotation about the N-C(O) bond in AA was obtained by analysis of the lineshapes of the amino-protons in AA. The behavior of the internal rotation as well as chemical shifts of the amino-protons in AA has been found to be closely related to the $E_{T}$(30) of n-alcohols.

• Parasites, Hosts and Diseases
• /
• v.35 no.1
• /
• pp.55-62
• /
• 1997

The molecular weight 30 kDa membrane protein of Toxoplusma Sondii (Toxoplasma 30 kDa) apparently conserved in most strains of T. gondii and sera of infected hosts. The present study aimed to elucidate Toxoplasmc 30 kDa as a useful diagnotic antigen for serodiagnisis of toxoplasmosis by ELISA and for induction of protective immunity. Murine spleen cells immunized with the membrane antigen of T. gondii were fused with mouse Sp2/0-Ag 14 myeloma cells. Out of 8 clones selected, five were IgG2b, the others belonged to IgG 1 and IgG2a. The 30 kDa antigen was distributed mainly on the surface membrane of tachyzoites by indirect fluorescence method. Murine peritoneal macrophages which were activated by 30 kDa antigen produced more amounts of NO2 compared with crude antigen-treated group, however there were no significant differences in toxoplamacidal activity between the two groups. Higher specificity of Toxoplosma 30 kDa antigen was recognized for serodiagnosis of toxoplasmosis than the crude antigen. From these results, ToxopLasmo 30 kDa antigen enhances the cytotoxic effect of macrophages as well as a more reliable means for the serodiagnosis of toxoplasmosis by ELISA. Key words: Toxoplosma gondii, 30 kDa antigen (p30), mouse, serodiagnosis, macrophage, cytotoxicity.

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.62-65
• /
• 2002

We have previously shown that the addition of silkworm hemolymph to a culture medium increases the longevity of insect and mammalian cells by inhibiting apoptosis. This indicates that the component which inhibits apoptosis is contained in the silkworm hemolymph, The apoptosis-inhibiting component was isolated from silkwonn hemolymph and characterized in our previous study. A database search using the N-terminal amino acid sequence of this component as a template resulted in a 95% homology with a low molecular weight lipoprotein, the so called ’30K protein' of unknown function. In this study, the 30K protein gene was expressed in mammalian and insect cells to confirm the apoptosis-inhibiting effect. The overexpression of 30K protein in mammalian cell inhibited the staurosporin-induced apoptosis by the prevention of the activation of caspase 3. Using an Autographa californicanuclear polyhedrosis virus (AcNPV) system, the 30K protein was overexpressed also in insect cells. The expression of the 30K protein increased the longevity of baculovirus-infected insect cells by inhibiting apoptosis. These results suggest that the 30K protein is a novel anti-apoptotic protein.

• Journal of the Korea Convergence Society
• /
• v.10 no.3
• /
• pp.383-393
• /
• 2019

The present study was conducted to determine convergent factors affecting depression in drinker by age. This was a cross-sectional secondary data analysis study that used the raw data from the Korea National Health and Nutrition Examination Survey in 2014 & 2016. The subjects of the present study were 4,446 middle-aged drinker. Variables related to general characteristics, physical and psychological factors were selected. After a sampling plan compound file was created using IBM SPSS 23.0 program, data were analyzed by giving weights. Depression was influenced by gender (30s, 50-60s), income (30-40s, 60s), education (30-40s), number of family members (30-60s), job (30-60s), eating level (30-40s, 60s), hypertension (40s), DM (30-40s), BMI (30-40s), smoking (30s, 50s), exercise (30s), weight control (60s), drinking binge (30-60s), subjective health (30-60s), subjective body awareness (30-50s), stress (30s-60s), quality of life (30-60s). It is required to design preventive interventions and management plans for depression, considering these factors comprehensively.

• IMMUNE NETWORK
• /
• v.3 no.1
• /
• pp.8-15
• /
• 2003

Background: CD30 is a member of TNF receptor family and expressed on lymphocytes and other hematopoietic cells following activation as well as Hodgkin and Reed-Sternberg cells in Hodgkin's lymphoma. In this study, CD30-mediated regulation of cell adhesion molecule expression on normal activated mouse T cells was investigated. Methods: Mouse T cells were activated with anti-CD3 antibody for induction of CD30, which was cross-linked by immobilized anti-CD30 antibody. Results: High level of CD30 expression on T cells was observed on day 5, but only little on day 3 even under culture condition resulting in an identical T cell proliferation, indicating that CD30 expression requires a prolonged stimulation up to 5 days. Cross-linking of CD30 alone altered neither proliferation nor apoptosis of normal activated T cells. Instead, CD30 appeared to promote cell adherence to culture substrate, and considerably upregulated ICAM-1 and, to a lesser extent, ICAM-2 expression on activated T cells, whereas CD2 and CD18 (LFA-1) expression was not affected. None of cytokines known as main regulators of ICAM-1 expression on tissue cells (IL 4, $IFN{\gamma}$ and $IFN{\alpha}$) enhanced ICAM-1 expression in the absence of CD30 signals. On the other hand, addition of $NF-{\kappa}B$ inhibitor, PDTC (0.1 mM) completely abrogated the CD30-mediated upregulation of ICAM-1 expression, but not CD2 and ICAM-2 expression. Conclusion: This results support that CD30 upregulates ICAM-1 expression of T cell and such regulation is not mediated by higher cytokine production but $NF-{\kappa}B$ activation. Therefore, CD30 may play important roles in T-T or T-B cell interaction through regulation of ICAM-1, and -2 expression.

• Journal of Korea Foundry Society
• /
• v.23 no.2
• /
• pp.77-85
• /
• 2003

Some alloying elements such as Cr, B, Ti and Si were added individually or as a mixture to Fe-30 at.%Al alloys. The alloys were melted using an arc furnace and then heat-treated for homogenization at 1000$^{\circ}C$ for 7 days and followed by rolling at 1000$^{\circ}C$. The alloying elements on rolling characteristics were investigated by the microstructures and fracture mode before and after rolling. The microstructures before rolling showed that all of the alloys had equiaxed grains. On the other hand, the microstructures of rolling plane as well as its perpendicular plane became elongated after rolling. The alloys such as Fe-30Al, Fe-30Al-3Ti, Fe-30Al-0.5B, Fe-30Al-5Cr and Fe-30Al-3Ti-0.5B revealed better rolling behaviour from the point that intergranular and cleavage fractures were not fundamentally occurred. But the addition of 5Ti or 3Si to Fe-Al alloys had detrimental effects. The Ti-added alloy system such as Fe-30Al-5Ti, Fe-30Al-5Ti-5Cr, Fe-30Al-3Ti-5Cr and Fe-30Al-5Ti-0.5B were cracked through grain and showed cleavage fracture. The Si-added alloy system such as Fe-30Al-5Si, Fe-27Al-3Si and Fe-27Al-5Cr-3Si were cracked along the grain boundary and showed intergranular fracture. $DO_3{\leftrightarrow}B_2$ transition temperature of Fe-30at.%Al alloy was 520$^{\circ}C$, whereas the addition of 3Ti and 3Ti+0.5B comparably increased the temperature to 797 and 773$^{\circ}C$, respectively.

• The Korean Journal of Food And Nutrition
• /
• v.33 no.3
• /
• pp.237-250
• /
• 2020

In this study, the Korea National Health and Nutrition Examination Survey in 2016 examined the nutrient and food intake of women in their 20s and 30s according to the number of meals a day, and the results were as follows: The study included 256 women in their 20s and 474 women in their 30s. Women in their 20s and 30s ate two meals a day, indicating that the notion of three meals a day was gradually changing to two to three meals a day. Those in their 20s and 30s who eat one, two or three meals a day were consuming less than the estimated energy requirement of the DRI for Koreans 2015. The vitamin A and C were eating less than the recommended intake in all meals. In the case of minerals, calcium was taken less than the recommended intake in all meals, but sodium was taken above the goal intake in all groups. Women in their 20s and 30s must reduce their intake of saturated fatty acids and sodium, and increase their intake of vitamins A, C, calcium, and potassium. Women in their 20s and 30s drank a lot of coffee, ate baechu-kimchi and rice frequently per week.