• Journal of Applied Biological Chemistry
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• v.66
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• pp.244-249
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• 2023

Artemisia keiskeana and A. stolonifera are plants of the genus Artemisia, distributed in various regions, especially China and Korea. They are renowned as medicinal plants with biological and pharmacological activities. Fraxidin, isofraxidin, and daphnoretin are coumarins present in Artemisia spp.; however, research on them is limited. Therefore, this study was carried out to quantify the content of these compounds in the aerial parts of A. keiskeana and A. stolonifera in different regions in Korea. High-performance liquid chromatography was performed with a photodiode array detector and a reverse-phase INNO column. A. stolonifera only contained fraxidin with the highest amount found in Yongmun commune. A. keiskeana cultivation in Soyang commune gave the highest fraxidin and daphnoretin content. However, isofraxidin was not present in all samples. The findings suggest that the concentrations of the three compounds may differ depending on the growth site and provide a foundation for future studies.

• FLOWER RESEARCH JOURNAL
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• v.18 no.1
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• pp.29-32
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• 2010

This study was conducted to determine the effects of cutting position, media, and auxin on rooting of Artemisia keiskeana cuttings. In the cutting positions, the cutting of middle and basal positions had a better growth in the both upper and lower part than the cutting of the top position. Especially, the cutting of the middle position showed the highest rooting rate, at 68.3%. In the media, peatmoss showed the latest date on the rooting initiation. Also, the growth, survival rate and rooting rate of the upper part and lower part were low. However, the cutting in vermiculate showed the fastest date on the rooting initiation, and had a significant effect on the growth of the upper part and lower part, showing the highest rooting rate and survival rate in all tested media. When cuttings were treated by auxin, the rooting rate and growth of cuttings were higher than the control. The control showed the lowest rooting rate, at 41%, while, the auxin treatment showed the highest, rate over 80%. Whereas when treated by NAA, the lower part of cuttings had a better growth than these treated by IBA. Also, the higher the concentration of auxin was, the higher the rooting rate and growth rate of Artemisia keiskeana cuttings were.

• Korean Journal of Plant Resources
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• v.10 no.4
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• pp.422-427
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• 1997

Vegetation and environment in natural habitats of Aster scaber and Epimedium koreanum around Chunchon was studied to eatablish agroforestry technology for high quality production of wild vegetable. The number of species within the quadrat in natural habitat of Aster scaber was respectively 20, 26, and 25 in Chunchon A, Mt. Yeonyup A, and Mt. Yeonyup B. The number of species within the quadrat in natural habitat of Epimedium koreanum was respectively 15, 32, and 26 in Chunchon B, Bukbangmyon, and Mt. Taeryong. The dominant species in habitat of Aster scaber was respectively Artemisia keiskeana, Disporun smilacinum, and Calamagrostis arundinacea in Chunchon A, Mt. Yeonyup A, and My. Yeonyup B while that in habitat of Epimedium koreanum was respectively Pteridium aquilinum. Artemisia keiskeana, and Disporun smilacinum in Chunchon B, Bukbangmyon, and Mt. Taeryong. Soil pH in habitats of Aster scaber and Epimedium koreanum ranged from 4.5 to 5.3. The contents of organic matter in habitat soil ranged from 1.5% in Chunchon A to 5.5% in Mt. Yeonyup B. The contents of ammonia-nitrogen and nitrate-nitrogen in soil were respectively 0.0043 mg/kg in habitat of Aster scaber and 0.0025mg/kg in habitat of Epimedium koreanum.

• Korean Journal of Plant Taxonomy
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• v.39 no.4
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• pp.247-253
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• 2009

Somatic chromosome numbers for 20 taxa of Korean Artemisia L. were investigated for the purpose of classification. Somatic chromosome numbers of treated taxa were 2n = 16, 18, 34, 36, 50, 52, 54, and therefore their basic chromosome numbers were x = 8, 9, 10, 13, 17. The chromosome number of A. japonica var. angustissima is being reported for the first time in this study. The chromosome numbers of 13 taxa were the same as in previous reports; A. capillaris (2n = 18), A. japonica var. hallaisanensis (2n = 36), A. japonica subsp. littoricola (2n = 36), A. annua (2n = 18), A. carvifolia (2n = 18), A. fukudo (2n = 16), A. keiskeana (2n = 18), A. stolonifera (2n = 36), A. sylvatica(2n = 16), A. selengensis (2n = 36), A. montana (2n = 52), A. lancea (2n = 16), A. sieversiana (2n = 18); however, the chromosome numbers of 6 taxa were different; A. japonica var. japonica (2n = 18, 36 vs 2n = 36), A. sacrorum (2n = 18, 54 vs 2n = 54), A. rubripes (2n = 16, 34 vs 2n = 16), A. indica (2n = 34, 36 vs 2n = 34), A. codonocephala (2n = 18, 50, 54 vs 2n = 50), A. argyi (2n = 34, 36, 50 vs 2n =34). The somatic chromosome numbers of Korean Artemisia are thought to be good characteristics for classifying some taxa such as A. japonica var. japonica, A. sacrorum, A. codonocephala, A. argyi, A. montana, A. sylvatica.

• The Korean Journal of Ecology
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• v.24 no.4
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• pp.205-211
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• 2001

The floristic composition and structure of the Oriental Arbor-viate forest, natural monument no. 1, were investigated in the Daegu city, southeastern Korea. Vegetation stratification have four layers including tree (＞5m), subtree (2m-5m), shrub (1m-2m) and herbs (＜1m), which occupied 63.8%, 10.7%, 12.1% and 49.4%, respectively. The vegetation of the study area were divided into Artemisia keiskeana - Quercus variabilis and Pyrrosia linearifolia-Thuja orientalis (OAV) communities. Frequency distribution for diameter classes of the Oriental Arbor-vitae population showed a reverse-J shape. The result suggests that the OAV forest of this site might maintains continuously the present state. The annual radial growth of OAV, Pinus densiflora and Quercus variabilis, the dominant species of the present site showed 0.29, 1.01 and 1.28 mm/year, respectively. Competition of OAV with theh other species including P. densiflora and Q. variabilis could influence negatively on the growthand survival of OAV forest in this site.

• Journal of Plant Biology
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• v.15 no.1
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• pp.1-12
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• 1972

As part of the study of the structure and the successional trends of our temperate forests, a quantitative survey was made of the relatively well conserved forest stands on Mt. Kwanak, Seoul. Three community types were observed; the Pinus densiflora-Quercus mongolica community, the Pinus densiflora community, and the Quercus mongolica community. Evidence suggests that the dominance of Pinus densiflora and quercus mongolica will continue in the Pinus densiflora-Quercus mongolica community, however, in the Pinus densiflora community the dominant species, Pinus densiflora, is now being replaced by Quercus mongolica. The trend toward an increased in Acer pseudo-sieboldianum var. koreanum, Fraxinus rhynchophylla, Carpinus cordita, and Acer mono is observed in the Quercus mongolica community. An analysis of the overall composition of the forest reveals that the upper story is mostly occupied by Quercus mongolica and Pinus densiflora, the shrub layer by Rhododendron schlippenbachii and Lespedeza maximowiczii, and the herb layer by Spodiopogon cotulifer and Artemisia keiskeana. Quercus mongolica may cover most part of the forest, gradually excluding Pinus densiflora. Acer pseudo-sieboldianum var. koreanum, Fraxinus rhynchophylla, Carpinus coradata, and Acer mono are increasing in importance with the potentiality of developing into major canopy components. With the diversification of the physical habitat Cephalotaxus koreana and Magnolia sieboldii, the species which had almost completely been eliminated form the forest through the severe interferences, have reappeared.

• The Korean Journal of Ecology
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• v.11 no.4
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• pp.213-225
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• 1988

The plant communities of the eastern ridge in Mt. Cheonma, Central Korea, Quercus mongolica, Q mongolica-Acer pseudo-sieboldianum, and Q. mongolica-Rhododendron schlippenbachii communities in the northern slope and Q. variabilis and Q. cutissima communities in the southern slope were detected by Zurich-Montpellier method. The undergrowth of Q mongolica community in the northern slope was dominated by Ainsliaea acerifolia, Carex siderosticta, Astilbe chinensis var. davidii, Heloniopsis orientalis, Pseudostellaria palibiniana, ranging 3.3 to 2.2 in dominance-sociability, while the those of Q variabilis and Q acutissima in the southern slope were dominated by Spodiopogon cotulifer, Artemisia keiskeana, Carex humilis, Atractylodes japonica, Lysimachia clethroides and Disporum smilacinum with similar degree in dominance-sociability to that of Q mongolica community. The two communities were considered as regenerating forest after severe disturbance judging by their age structure. The relationship between Shannon's diversity index (H') and Simpson's dominance index (i) was in reciprocal proportion in both two communities. The Q. mongolica and Q. variabilis communities were showed 13 and 12 m respectively. Seasonal changes of undergrowth coverage in the two communities were seemed to be affected especially with soil moisture among environmental factors such as temperature, light intensity, relative humidity and organic matter.

• The Korean Journal of Ecology
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• v.10 no.2
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• pp.63-80
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• 1987

Detrended correspondence analysis(DCA) and two way indicator species analysis(TWINSPAN) were applied to Pinus densiflora stands nearby Seoul area in order to classify them into several groups based on herbaceous species and environmental relationships. The eighteen Pinus densiflora stands were perferenially selected, In each stand, 30 to 60 quadrats, (20X20)cm, were randomly estabilished. In each quadrat, all stems for herbaceous plants were recorded by species and their covers were measured. In addition environmental factors such as soil depth, thickness of litter layer, soil compressibility, tree density, basal area, elevation, slope and exposure were detemined for each stand. Four to twelve soil cores were collected and physical and KDICical properties were determined. This survey was conducted form 15, July, 1985 to 22, September, 1985. Analysis of the vegetation and environmental data were performed with DECORANA and TWINSPAN. Stand ordination (DECORANA) and classification (TWINSPAN) based on herbaceous species suggest that the stands can be classified into five groups in terms of herbaceous species composition and environmental relationships. Type I, Type II, Type III, Type IV and Type V were classified to SUBMESIC-CLO-SED-CANOPY (Artemisia keiskeana, Carex nanella), MESIC-CLOSED CANOPY (Spodiopogon sibiricus, Miscanthus sinensis, Carex nanella, Pteridum aquilinum, Melampyrum rosium), SUBMESIC-OPEN CANOPY (Carex nanella, Spodiopogon sibiricus, Miscanthus sinensis), XERIC-CLOSED CANOPY (Carex nanella, Spodiopogon sibiricus, Carex nanella), and XERIC-OPEN CANOPY (Arundinella hirts, Spodiopogon sibiricus, Carex nanella), respectively. These results were consitent with environmental trends on stan ordination. Application of the ordination and classifiaction to Pinus densiflora stands were effective as a approach on obtaining perliminary ecological information.

• Preventive Nutrition and Food Science
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• v.7 no.4
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• pp.358-366
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• 2002

To search for novel cancer preventive agents, we assessed the quinone reductase (QR)-inducing activities of medicinal herb extracts in cultured murine hepatoma cells (hepalclc7 cells). Among 216 herb extracts tested in this study, 8 kinds of herbal extracts were found to induce QR activity in hepalclc7 cells by more than 2-fold when used at the concentration of 25 $\mu\textrm{g}$/$m\ell$. The methanol extracts of Aster koraiensis NK and Pulsatilla koreana Nakai induced QR by 252 and 223 % , respectively, at the concentration of 25 $\mu\textrm{g}$/$m\ell$. Most of the herbal extracts with QR inducing-activity increased the enzyme activity in a typical dose-dependent manner. The QR activity in BP$^{r}$ cl cells was induced move than 50 % by the extracts of Pulsatilla koreana Nakai, Inula helenium, Physalis alkekengi var, francheti (Masters) Makino, Chrysanthemum zawadskii var. latilobum Kitamuva, Auemisia keiskeana Miquel, Chfsanthemum boreale Makino. In conclusion, hlsatilla koreana Nakai, Aster koraiensis N.K, and Chfsanthemum zawadskii var. iatilobum Kitamura, which showed relatively high QR induction, merit further animal study to evaluate their potential as cancer preventive agents.

• Journal of Korean Society of Forest Science
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• v.107 no.1
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• pp.25-34
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• 2018

This study was performed to discover the comparison of the understory species composition and the growth velocity of the regeneration seedlings in Pinus densiflora forests being managed by the seed-tree method from 2008 year. The investigation site located in Bonghwa, Gyeongsangbuk-do province was established by eighteen quadrats such as six control ones, six slope ones and 6 concave ones, in which we carried out the vegetation survey and measured annual height growth of each seedlings. As a result of vegetation analysis, the understory vegetation of the seed-tree forest area was mainly composed of shrub plants such as Lespedeza bicolor, Zanthoxylum schinifolium, Securinega suffruticosa, Lespedeza maximowiczii and Stephanandra incisa, herb plants such as Miscanthus sinensis var. purpurascens, Carex humilis var. nana, Spodiopogon sibiricus and Artemisia keiskeana, and vine plants such as Pueraria lobata, Smilax sieboldii, Dioscorea batatas, Actinidia arguta, Vitis amurensis and Rubus crataegifolius. Especially, the vine plants were relatively more imported to the concave site than the control site and the slope site. As a result of measurement of growth velocity, the seedlings of the Pinus densiflora appeared to be 3,175 trees/ha for the non-suppressed trees and 7,842 trees/ha for the suppressed trees. In cases of the concave site, individuals of seedlings were much lower than those on the slope site, probably due to the competition effects of vine plants. Consideringly, silvicultural practices for clearing the vine plants should be inevitably accompanied. Also, the growth velocity of the suppressed and non-suppressed trees has been constantly increasing, those of the non-suppressed trees much higher than the suppressed trees. This represents that the seedlings grow rapidly after the suppressed period with competition to the surrounding plants. Therefore, specific practices such as blush control and vine clear should be carried out at the beginning stage of regeneration in the seed-tree stand.