• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.3
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• pp.161-167
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• 1998

Root and shoot development of two rice (Oryza sativa L.) cultivars with different genetic backgrounds was studied with reference to their relative growth. Tongil type (indica-japonica hybrid) cultivar 'Kuemkangbyeo' and japonica cultivar 'Koshihikari' were grown in $5000^{-1}$ a Wagnar pots under flooded condition. Three plants with roots of both cultivars were taken in every phyllochron through the heading stage to record morphological characteristics of shoot and root system. Compared to Koshihikari, Kuemkangbyeo produced more tillers and had greater shoot weight and leaf area per hill. Length and weight of the root system in both cultivars increased exponentially with time. At the same time, root system development was significantly faster in Kuemkangbyeo than in Koshihikari after the panicle initiation stage. As a result, Kuemkangbyeo has a vigorous root system which consists of larger number of nodal roots compared to Koshihikari. Also, the root length and weight per unit leaf area of Kuemkangbyeo were larger than those of Koshihikari in the later half of growing period, which suggests possible higher physiological activity of the root system of Kuemkangbyeo which is known as a high-yielding cultivar. The relationship between root traits (crown root number, total root length, and root dry weight) and shoot traits (leaf area and leaf+culm dry weight) in both cultivars closely showed allometry until the flag leaf stage.

• Korean Journal of Breeding Science
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• v.42 no.1
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• pp.23-27
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• 2010

The objective of this study was to map the gene for reduced embryo size in rice using DNA markers. The reduced embryo size mutant was induced from N-methyl-N-nitrosourea (MNU) treated Taichung 65. Genetic analysis revealed that the phenotype of the reduced embryo was controlled by a single recessive gene, designated as re-1. For mapping the gene controlling embryo size, an $F_2$ population was developed from a cross between the Korean Tongil-type, Milyang 23 (Oryza sativa ssp. indica) and the mutant. The ratio of $F_2$ seeds nearly fitted to 3:1 ratio, indicating that this phenotype was controlled by a single recessive gene. Bulked sergeant analysis was performed with SSR markers. The gene for the reduced embryo size was detected on chromosome 1. The gene was further mapped between two SSR markers, RM315 and RM265 on chromosome 1 (approximately 1.5 Mb interval). The linked markers will facilitate selection of this grain character in a breeding program and provide the foundation for positional cloning of this gene.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.2
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• pp.101-107
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• 2021

The objective of this study was to analyze social wasps' urban distribution tendency based on 10 species found in two metropolitan cities (Busan and Daegu) of South Korea. There 10 species included six species (Vespa mandarinia, V. ducalis, V. crabro flavofasciata, Vespula koreensis koreensis, Parapolybia indica, and Polistes snelleni) of forest dwellers that inhabited urban main forests and satellite forests, two species (V. simillima simillima and V. analis parallela) of facultative dwellers that nested at diverse sites of urban areas with greater preference for urban forest, and two species (V. velutina nigrithorax and P. rothneyi koreanus) of urban dwellers that nested at almost all sites, including urban and forest areas. These urban dwellers were found to adapt well to an urban environment based on their far higher rate of urban nesting compared to facultative dwellers. When distribution tendencies of facultative dwellers and urban dwellers in Busan and Daegu were compared, a regular distribution was mostly observed in Busan with a dense forest network. For Daegu that lacked forest connectivity, the greatest distribution of species was found in the nearby urban forest. For Daegu, a city further away from forests, urban dwellers occurred far beyond forest sites compared to Busan with a dense forest network.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.217-217
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• 2022

Pullulanase (PUL), a debranching enzyme, has been utilized in hydrolyzing the a-1,6 glucosidic linkages in starch, amylopectin, pullulan, as well as related oligosaccharides. It has also been indicated that PUL is a novel indicator of inherent RS (Resistant Starch) formation in rice. In this study, we performed haplotype analysis on 320 bred rice accessions, and additional 54 wild accessions were added to study genetic diversity along with other population-based analyses of the PUL gene. Through these investigations, we summarized a total of 10 functional (non-synonymous) SNPs from 7 different exons on chromosome 4. There were 10 haplotypes, of which only six haplotypes were functional, implicating different subpopulations. Diversity reduction was noticed in temperate japonica (0.0005) compared to the highest one (aus, 0.0154), illustrating their higher genetic differentiation by F_ST-value (0.926). The highest Tajima^ D value was observed in indica (3.6613), indicating PUL gene domestication signature under balancing selection, while the lowest Tajima's D value was found in temperate japonica (-2.2191) which might have undergone under positive selection and purified due to the excess of rare alleles. PCA, population structure, and phylogenetic analyses provide information on the genetic relatedness between and or among the cultivated subpopulations and the wild based on PUL genomic region.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.2
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• pp.201-206
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• 1985

This experiment was carried out to get the basic information about long term storage (Temp.: -10${\pm}$1$^{\circ}C$ and -l${\pm}$1$^{\circ}C$, RH; 30${\pm}$6) of rice germplasm by using three Japonica and one Indica x Japonica cultivars based on the storage periods (96, 86, 58 and 20 months). The germination ability, based on the storing periods, was tested under the conditions of 30-32$^{\circ}C$ and 15-17$^{\circ}C$ air temperature. The results obtained are summarized as follows: 1. There were no significant differences between the short and long term storage conditions in the percentage of germination, average germination period, germination coefficient of the four varieties tested under both 15-17$^{\circ}C$ of low and 30-32$^{\circ}C$ of optimum temperature conditions. 2. Eventhough there were no significant differences in germination depending on the storage periods under optimum temperature condition (30-32$^{\circ}C$). Longer storage duration resulted in lower germination percentage, longer average germination period and lower germination coefficient under low temperature condition (15-17$^{\circ}C$). Comparing the varieties, the germination percentage of a Indica/Japonica cultivar "Tongil" was lower than that of Japonica cultivars under the low temperature condition (15-17$^{\circ}C$). 3. The longer period of storage, the more abnormal plants had appeared. 4. The germination ability was lost earlier under the condition of high moisture content in the seed and non-ventilation container.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.3
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• pp.244-249
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• 1992

To investigate the varietal variations in absolute density of milled rice grain and its relations with other grain characters, the 235 rice varieties which consist of 30 Tongil type rices, 72 Japonica rices and 133 Indica rices were tested on grain size, volume, hardness, chalkiness and 1000 grains weight of brown rice, ADV, amylose content, starch composition on SEM and absolute density of milled rice grain using completely ripened grains. Average absolute density of milled rice grain was 1.496g / cm$^3$ in Tongil type rices, 1.506g / em in Japonica rices, and 1.500g / cm$^3$ in Indica rices. It was correlated positively with days to heading and grain hardness, and negatively with chalkiness, volume, grain weight and grain length of brown rice. Regression analysis indicated that grain volume and weight were the major characters affecting the density. However, since the absolute density of milled rice grain did not show great varietal variations it might not seem important as one of the characters contributing to the grain yield, while it could be a factor affecting the grain quality because there were definite varietal differences even though small. The microscopic feature of starch composition on SEM revealed differences between clear and chalky parts of the grain in shape and compactness of starch composition but did not discriminate between high and low-density grains.

• Korean journal of applied entomology
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• v.4
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• pp.51-54
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• 1965

The study was initiated to investigate the nature of varietal resistance to the rice stem borer (Chilo suppressalis WALKER), specially the rotation of the resistance to the silica and nitrogen content of the host Plants. 1. The body freight of the larvae, reared on the stem of the various varieties of rice plants, when weighed at the age of 25 days old, showed great variation, and ranged from 21.24 mgrs. to 45.77 mgrs. depending on the used varieties of tile rice plants. And generally the varieties of Indica types were showed greater body weight than that of Japonica types in this experiments. 2. The positive correlation coefficients of percent survivals to body weight, developement, oviposition preference and rate of damage to host plants were significant, and consequently the percent survival could be used as a criteria of resistance. 3. The content of silica in the host plants was significantly correlated with both the degree of boring on the leaf and attractovemess of larvae, and the total nitrogen content of plants showed significant positive correlation coefficient with larval attractivenes, but not significant with the degree of boring on the leaf. 4. Since the attractiveness and boring manner were significantyly correlated to resistance criteria of rice plant to the pest, it could be considered that the resistance is the results of chain reaction of the factors mentioned above.

• Korean Journal of Agricultural Science
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• v.9 no.2
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• pp.569-575
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• 1982

Mechanical properties of rice plants were tested to determine compressive force, bending force, tensile force and shear force for improvement of harvesting machines and for efficient utilization of rice culm during the proper harvesting period. Rice varieties used in this study were two Japonica varieties with Irri 348 and Jinju, and two $Indica{\times}Japonica$ hybrids with Seogwang and Taebaeg, which were grown in the standard fertilization field of Chungnam Rural Development Office. Also Jinju and Taebaeg were tested to elucidate the shearing characteristics which included shear force-strain relationship, shear force and shear energy according to the position from the ground level, the shearing angle to the rice culm, and the moisture content. 1. Compressive force, bending force, tensile force and shear force were higher In Japonica varieties than $Indica{\times}Japonica$ hybrids. 2. Shear force to overall culm length decreased progressively to upper positions in Jinju variety but a constant shear force was approximately showed between the ground level and the position of 21cm in Taebaeg variety. 3. Shear force and shear energy increased with increase of the cross sectional area, and the rates of increase were high in general up to the cross sectional area of $10mm^2$ and then they became dull very much. 4. Shear force and shear energy decreased with decrease of moisture content of rice culm after cutting up to the moisture content of 60% (w. b.) and then they did not change significantly.

• Korean Journal of Environmental Agriculture
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• v.33 no.2
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• pp.138-143
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• 2014

BACKGROUND: Azadirachta Indica extract(AIE) and Sophorae radix extract(SRE) are widely used as environment-friendly organic materials of plant origin in South Korea. METHODS AND RESULTS: In this study, the in vitro micronucleus(vitMN) tests of two samples of AIE and SRE were conducted to evaluate their genotoxicity using the Chinese hamster lung(CHL) cell. This study was composed of two parts; cytochalasin B(cyto B) test and non-cyto B test. Mitomycin C and colchicine were used as positive controls. As a result, the incidence of micronucleus(MN) in all AIE and SRE treated groups increased in dose-dependent manner, but were less than 2.2% in 1,000 binucleated cells. In addition, there were no significant increases of MN incidence in all AIE and SRE treated groups, compared with the negative control group. CONCLUSION: Therefore, we suggest that AIE samples and SRE samples used in this study may have no genotoxicity in the in vitro micronucleus test using the CHL cells. In our previous study, we reported that AIE and SRE did not cause genotoxicity in Ames test. According to the genotoxicity battery system, we concluded that AIE and SRE used in this study have no genotoxic effects to humans.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.12
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• pp.55-62
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• 1972

In order to study the biolgiocal effects of dry back following EMS (Ethyl methane sulfonate) treatment on seeds of 'Dunghan Shali' that belongs to indica-type rice with various presoaking(24, 36, and 40 hrs respectivily) and treatment time(l, 2 and 3 hrs respectivily), this experiment was done. Seedling heights of wet seeds presoaked for 36 hrs was only greatly decreased with EMS treatment compared with other periods. In germination rate of wet seeds, all of these damage was not changed. For the drying back seeds, otherwise, seedling height and germination rate were greatly reduced depend upon the time of treatment and presoaking as well as storage effects after drying back. Reduction patterns, both seedling height and germination rate, between 1 week and 8 weeks after dry back were quite similar. The other experiment was carried out in order to study for the influences on the seedling growth as biological damage with EMS and MMS(Methyl methane sulfonate) treatments among different varieties. The indica-type rice varieties; Taichung Native No.1, Dunghan Shali and IR-8, were used as the experimental materials. From this trial some results were obtained as follows; 1. Both seedling growth and germination rate on all varieties, used, MMS, treatment showed more toxic effects than those of EMS treatment. 2. Seeding growth injury of rice seeds dried back was increased gradually, and then was approached the maximum at 6-8 days after storing. In IR-s variety, otherwise, growth damage was appeared a little. Germination rate of these were quite smilar, even though chemical used is different and storage period was prolonged. 3. It was showed clearly that varietal differences of chemical mutagene treatments were recognized.