Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Genotoxicity of Environment-friendly Organic Materials of Plant Origin in the Micronucleus Test Using Chinese Hamster Lung Cells

Chinese Hamster Lung Cell의 소핵시험을 이용한 식물추출물 유기농업자재의 유전독성평가

  • Cho, Hyeon-Jo (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Park, Kyung-Hun (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Jeong, Mi Hye (Agro-Material Safety Evaluation Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Park, Soo Jin (Korea Bio-Safety Institute Co., ltd.) ;
  • Oh, Jin-Ah (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Won-Il (Microbial Safety Team, National Institute of Agricultural Science, Rural Development Administration) ;
  • Cho, Namjun (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Ryu, Jae-Gee (Microbial Safety Team, National Institute of Agricultural Science, Rural Development Administration) ;
  • Paik, Min-Kyoung (Chemical Safety Division, National Institute of Agricultural Science, Rural Development Administration)
  • 조현조 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 박경훈 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 정미혜 (농촌진흥청 국립농업과학원 농자재평가과) ;
  • 박수진 (한국생물안전성연구소) ;
  • 오진아 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 김원일 (농촌진흥청 국립농업과학원 유해생물팀) ;
  • 조남준 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 류재기 (농촌진흥청 국립농업과학원 유해생물팀) ;
  • 백민경 (농촌진흥청 국립농업과학원 화학물질안전과)
  • Received : 2014.05.08
  • Accepted : 2014.06.10
  • Published : 2014.06.30
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Abstract

BACKGROUND: Azadirachta Indica extract(AIE) and Sophorae radix extract(SRE) are widely used as environment-friendly organic materials of plant origin in South Korea. METHODS AND RESULTS: In this study, the in vitro micronucleus(vitMN) tests of two samples of AIE and SRE were conducted to evaluate their genotoxicity using the Chinese hamster lung(CHL) cell. This study was composed of two parts; cytochalasin B(cyto B) test and non-cyto B test. Mitomycin C and colchicine were used as positive controls. As a result, the incidence of micronucleus(MN) in all AIE and SRE treated groups increased in dose-dependent manner, but were less than 2.2% in 1,000 binucleated cells. In addition, there were no significant increases of MN incidence in all AIE and SRE treated groups, compared with the negative control group. CONCLUSION: Therefore, we suggest that AIE samples and SRE samples used in this study may have no genotoxicity in the in vitro micronucleus test using the CHL cells. In our previous study, we reported that AIE and SRE did not cause genotoxicity in Ames test. According to the genotoxicity battery system, we concluded that AIE and SRE used in this study have no genotoxic effects to humans.

님추출물과 고삼추출물은 식물추출물 유기농업자재로 우리나라에 등록되어 친환경농산물 재배에 널리 사용되고 있다. 본 연구에서는 님추출물 2종과 고삼추출물 2종에 대한 안전성을 확인하고자 Chinese hamster lung cells을 이용한 in vitro 소핵시험(vitMN)을 통해 유전독성을 평가하였다. 시험은 cytochalasin B 적용군과 비적용군으로 구분하여 시험하였으며, 양성대조물질로 mitomycin C와 colchicine을 사용하였다. 시험 결과, 님추출물 시료 2종과 고삼추출물 시료 2종은 처리 농도에 따라 소핵발생 비율이 증가하였으나 모든 시료 처리군에서 전체 발생률이 2.2% 미만으로, 음성대조군 대비 2배 미만의 소핵이 관찰되었다. 따라서 님추출물 및 고삼추출물 시료는 본 시험의 vitMN 결과 최종적으로 음성으로 확인되었다. 또한 본 연구와 동일한 시료를 이용한 선행연구에서 복귀돌연변이 시험에서 음성을 보인 결과를 근거로 battery system에 의거해 종합적으로 평가하였을 때 님추출물과 고삼추출물 시료는 유전독성을 일으키지 않는 것으로 최종 판정하였다.

Keywords

References

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