• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.18 no.3
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• pp.37-43
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• 2005

This study was performed to determine the cytotoxic effect of methanol extract from Houttuynia Cordata and Glycine soja. The cell viability was determined by MTT method. Their cytotoxic activities against three cancer cell lines such as A549, MDA-MB-231 and SNU-C4 cell line were tested. Among them, The methanol extract of Houttuynia Cordata showed the strongest cytotoxic effect against SNU-C4 cells. These results suggest that the methanol extract of Houttuynia Cordata possessed a potential antitumorous agent. The free radical scavenging activity using DPPH method was the strongest of Houttuynia Cordata methanol extract and ethyl acetate fraction.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.5
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• pp.1288-1292
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• 2003

This study was carried out to evaluate cytotoxic effects of Houttuynia cordata T/sub HUNB/ extracts on A549 (lung cancer), MDA-MB231 (breast cancer), SNU-C4 (colon cancer) and B16 (mouse melanoma) cell lines. We have determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The 150 ㎍/㎖ concentration of methanol extract (63.81 %) of Houttuynia cordata T/sub HUNB/ was shown significantly antitoxic activity on A549 cell lines. The order of cytotoxicity fractions of methanol from Houttuynia cordata T/sub HUNB/ extracts against cancer cell lines in vitro is as follows : hexane fraction layer > chloroform fraction layer > ethyl acetate fraction layer > buthanol fraction layer > water fraction layer. These results suggest that the hexane fraction of methanol extract from Houttuynia cordata T/sub HUNB/ extract may be a valuable choice for the development of antitumor agents.

• Korean Journal of Pharmacognosy
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• v.32 no.1 s.124
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• pp.61-67
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• 2001

This study was conducted to investigate the antitoxic agent in methanol extract of Houttuynia cordata $T_{HUNB}$. Detoxication effects By H. cordata $T_{HUNB}$ extract increased in proportion to the extract concentrations. When 40 mg/kg dosage of H. cordata $T_{HUNB}$ extract was administered, it showed the highest antitoxic effects in metallothionein induction. After the extract treatment, body weights generally increased in proportion to the extract concentrations. From the above results, H. cordata $T_{HUNB}$ extract increased metallothionein concentrations and decreased the toxicity of cadmium in rats. In vitro the antitoxic activity of methanol extract of H. cordata $T_{HUNB}$ on NIH 3T3 fibroblasts was evaluated by the MTT {3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide} and SRB (sulforhodamine B protein) assays. The light microscopic study was carried out to observe morphological changes of the treated cells, $10^{-2}\;mg/ml$ Concentrations of H. cordata $T_{HUNB}$ extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were increased and tend to regenerate. These results suggest that H. cordata $T_{HUNB}$ extract retains a potential antitoxic activity.

• Korean Journal of Medicinal Crop Science
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• v.18 no.5
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• pp.329-337
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• 2010

'Angelicae Pubescentis Radix' (APR) is an important oriental medical preparation. In Korea, Aralia continentalis has been recognized as the source plant of APR. Aralia cordata, which is difficult to distinguish from A. continentalis, and Heracleum moellendorffii, which is frequently used in lieu of A. continentalis, are traded in Korean herbal markets. In contrast, in China, Angelica pubescens is recognized as the source plant of APR. In this study, we devised a method not only to discriminate A. contientalis from A. cordata, but also to discriminate both A. contientalis and A. cordata from H. moellendorffii and A. pubescens. Based on the discrepancy in the sequences of specific regions of ITS, we designed a Cont F/ Cont R primer set to amplify a 173 bp PCR band that appears only in A. continentalis. Additionally, we designed an Ara F/ Ara R primer set to amplify a 278 bp PCR band that appears in both A. continentalis and A. cordata. Using these primer sets and the ST R primer to confirm the PCR amplification results, we developed a simple multiplex PCR method for differentiating A. continentalis from A. cordata and to concurrently differentiate both A. continentalis and A. cordata from other APR herbs.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.10
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• pp.1391-1396
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• 2011

Houttuynia cordata Thunb powder (0.05 g) was placed in a stainless vessel containing 10 mL of water, and subcritical water (SCW) extraction was carried out at various temperatures (50, 100, 200, and $300^{\circ}C$) for 10, 30, and 60 min. SCW treatment significantly affected physiologically important compounds, antioxidant activity, and acetylcholinesterase inhibitory activity of H. cordata Thunb extracts. H. cordata Thunb extracted at $300^{\circ}C$ for 30 min had the highest quercetin content, DPPH radical scavenging activity, total phenolic content, and acetylcholinesterase inhibitory activity, whereas the extract at $100^{\circ}C$ for 10 min possessed the highest flavonoid content. The highest ABTS radical scavenging activity was found in H. cordata Thunb extracted at 200 and $300^{\circ}C$ for 60 min. These results indicate that SCW extraction might be a useful processing method for obtaining valuable materials from H. cordata Thunb.

• Journal of Forest and Environmental Science
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• v.32 no.1
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• pp.74-81
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• 2016

Classified into sub-tree of the growth-form, Acer pseudosieboldianum and Carpinus cordata hardly reach the uppermost canopy with limited height growth potentiality in the natural deciduous forest. However, the two species usually dominate lower stories of the forest, playing an important role in overall ecological characteristics in the forest. Based on the vegetation data of 106 $20m{\times}20m$ sample plots in Mt. Jumbong area, this study was carried out to evaluate ecological constitution by several quantitative analysis so as to understand the mechanism of the natural deciduous forest. The results indicated that individuals of A. pseudosieboldianum and C. cordata were absent or few in overstory, but emerged the most dominant species in midstory and understory, providing adequate proof of the ecological importance. The comparison of indices of succession between presented and predicted values in midstory did not make much difference, suggesting that the species composition would not change much and come close to steady state in midstory and understory. The pair combination of species association noted that A. pseudosieboldianum had significant positive association with C. cordata, Quercus mongolica, and Tilia amurensis had significant positive association with A. pseudosieboldianum, A. pictum subsp. mono and Fraxinus mandshurica but negative association with F. rhynchophylla. Being compared with other major canopy tree species in the study forest, the target species of A. pseudosieboldianum and C. cordata had strong regeneration strategies, partially characterized by large number of saplings and pole sized trees and high ratio of live crown, which indicated high shade tolerance to survive in the limited amount of light under the canopy. Even though A. pseudosieboldianum and C. cordata do not reach and occupy the canopy layer mainly due to the inherent growth form, they would have highest competitive potentiality to prosper and dominate in the midstory of the natural deciduous forest.

• Journal of Environmental Science International
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• v.24 no.10
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• pp.1309-1313
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• 2015

The effects of Houttuynia cordata powder on the growth performance of ducks were investigated. Ninety ducks were assigned into one of three dietary treatments as a completely randomized design for 6 weeks: feeds supplemented with 1% or 2% H. cordata and a control group. No significant difference was observed in feed conversion among the three groups (p > 0.05), but addition of H. cordata had a significantly positive effect (p < 0.05) on initial and final body weight, weight gain, and feed intake of the ducks. Furthermore, the effects of chemical treatment (comprising 50 g and 100 g aluminum chloride [$AlCl_3$] per kilogram litter) on the ammonia ($NH_3$) flux in duck litters were also investigated. Duck litter was treated with $AlCl_3$ at a depth of 8 cm by top-dressing; this resulted in a significant difference on $NH_3$ flux (p < 0.05) during the experimental period (but not at 2 weeks). $NH_3$ flux at 6 weeks were reduced by 25.4% and 37.5% by treatment with 1% and 2% H. cordata, respectively, compared with the control groups. In conclusion, enriching the diets of the ducks with 2% H. cordata and adding 100 g $AlCl_3$ to their litter has beneficial effects on increasing their growth performance and reducing $NH_3$ flux in their environment.

• Journal of Acupuncture Research
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• v.22 no.2
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• pp.191-201
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• 2005

Background & Objective: Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycan and collagen in the cartilage extracellular matrix. The purpose of this study was to investigate the effects of Aralia cordata Thunb. in inhibiting the release of glycosaminoglycan (GAG), the degradation of collagen, and MMP activity in rabbit articular cartilage explants. Methods : The cartilage-protective effects of Aralia cordata Thunb. were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMP activity, measurement of lactate dehydrogenase activity and histological analysis in rabbit cartilage explants culture. Results : Interleukin-la (IL-1a) rapidly induced GAG, but collagen was much less readily released from cartilage explants. Aralia cordata Thunb. significantly inhibited GAG and collagen release in a concentration-dependent manner. Aralia cordata Thunb. dose-dependently inhibited MMP-3 and MMP-13 expression and activities from IL-1a-treated cartilage explants cultures when tested at concentrations ranging from 0.02 to 0.2 mg/ml. Aralia cordata Thunb. had no harmful effect on chondrocytes viability or cartilage morphology in cartilage explants. Histological analysis indicated that Aralia cordata Thunb. reduced the degradation of the cartilage matrix compared with that of IL -1a-treated cartilage explants.

• Journal of the East Asian Society of Dietary Life
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• v.12 no.1
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• pp.1-6
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• 2002

Houttuynia cordata Thunb. grown in wet earth in China, Japan and Korea, has often been cited in medical literature for its medicinal effects. In this paper, the pharmaceutical effects of Houttuynia cordata Thunb., featured in the East Asian literature were studied. It was revealed that it has a wide variety of uses, including remedial treat-ments for pneumonia, antidote, inflammation, syphilis, abscess, paralysis and gynecological diseases. Its application methods also are numerous: drinking as tea or squeezed juice, application to wounds, wet dressing, chewing raw roots and mixing with other plants, liquor and food materials. The major pharmaceutical effects are as follows: antibiosis, immunity enhancement, urination, pain relieving, vasodilator and cough lozenge.

• Biomedical Science Letters
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• v.24 no.2
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• pp.87-93
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• 2018

The current study was carried out to determine the effects of the leaf and root of Houttuynia cordata Thunb on antioxidant and anti-inflammatory. Total polyphenol contents of leaf and root ethanol extracts were found to be 59.32 and 12.07 mg/g, respectively. Also, total flavonoid contents of leaf and root ethanol extracts were found to be 10.85 and 8.55 mg/g, respectively. The $RC_{50}$ values of DPPH radical scavenging of leaf and root ethanol extracts were 23.51 and $154.72{\mu}g/mL$, respectively. The $RC_{50}$ values of ABTS radical scavenging of leaf and root ethanol extracts were 35.42 and $233.89{\mu}g/mL$, respectively. The antioxidant activities in leaf ethanol extracts were higher in root. Also, to confirm anti-inflammatory activity of ethanol extract, we treat leaf and root of Houttuynia cordata Thunb extract on BV-2 cell with LPS. The NO inhibition effects in of Houttuynia cordata Thunb leaf ethanol extracts showed higher values compared with the root ethanol extracts. These results indicate that Houttuynia cordata Thunb ethanol extracts may play a positive role in antioxidant and anti-inflammatory.